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Glycine in unfolding buffer - (Feb/11/2009 )

Hi all,

I'm unfolding my protein in buffer containing 50mM phosphate, 50mM glycine, 8M urea, and 1mM DTT. Could anyone explain the role of glycine in this buffer?



see my reply in your other posting (in the biochemistry forum). please don't post the same question in more than one forum.