What is the solution when tween80 or DMSO is not enough to dissolve plant extrac - (Jun/14/2010 )
Hi,
I'm stuck on this thought, can you please help me!?
I'm study the effect of some plant extracts (by organic solvent: hexan. chloroform, EA and methanol) on nematode larvae.... I have to use just 3% of DMSO or Tween80 to dissolve 5 grams of extract to prepare 100ml of 50mg/ml extract as stock by dissolving 5g of extract in 3ml of DMSO or Tween80 and add 97ml distilled water and then I'll use this concentrations 50, 25, 12.5, 6.3 and 3.2 but the problem is 3ml is not enough to dissolve 5g of extract and If I add more DMSO OR Tween it'll be toxic for the larvae?
So, how to solve this problem
Ahmed
Have you tried sonicating the extract in the 3mL of DMSO?
Ahmed Alrifai on Jun 14 2010, 12:37 PM said:
I'm stuck on this thought, can you please help me!?
I'm study the effect of some plant extracts (by organic solvent: hexan. chloroform, EA and methanol) on nematode larvae.... I have to use just 3% of DMSO or Tween80 to dissolve 5 grams of extract to prepare 100ml of 50mg/ml extract as stock by dissolving 5g of extract in 3ml of DMSO or Tween80 and add 97ml distilled water and then I'll use this concentrations 50, 25, 12.5, 6.3 and 3.2 but the problem is 3ml is not enough to dissolve 5g of extract and If I add more DMSO OR Tween it'll be toxic for the larvae?
So, how to solve this problem
Ahmed
Yes, I tried but to no avail
medchemgirl on Jun 14 2010, 10:22 AM said:
Ahmed Alrifai on Jun 14 2010, 12:37 PM said:
I'm stuck on this thought, can you please help me!?
I'm study the effect of some plant extracts (by organic solvent: hexan. chloroform, EA and methanol) on nematode larvae.... I have to use just 3% of DMSO or Tween80 to dissolve 5 grams of extract to prepare 100ml of 50mg/ml extract as stock by dissolving 5g of extract in 3ml of DMSO or Tween80 and add 97ml distilled water and then I'll use this concentrations 50, 25, 12.5, 6.3 and 3.2 but the problem is 3ml is not enough to dissolve 5g of extract and If I add more DMSO OR Tween it'll be toxic for the larvae?
So, how to solve this problem
Ahmed
Ahmed Alrifai on Jun 17 2010, 10:24 AM said:
medchemgirl on Jun 14 2010, 10:22 AM said:
Ahmed Alrifai on Jun 14 2010, 12:37 PM said:
I'm stuck on this thought, can you please help me!?
I'm study the effect of some plant extracts (by organic solvent: hexan. chloroform, EA and methanol) on nematode larvae.... I have to use just 3% of DMSO or Tween80 to dissolve 5 grams of extract to prepare 100ml of 50mg/ml extract as stock by dissolving 5g of extract in 3ml of DMSO or Tween80 and add 97ml distilled water and then I'll use this concentrations 50, 25, 12.5, 6.3 and 3.2 but the problem is 3ml is not enough to dissolve 5g of extract and If I add more DMSO OR Tween it'll be toxic for the larvae?
So, how to solve this problem
Ahmed
Sometimes it helps to use acetone as first solvent, because it is miscible in water and many lipophilic compounds are solved better in acetone...
