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RNA Related Discussions
  1. Can I snap freeze my tissue (liquid nitrogen) in Lysis buffer for later RNA extr - (reply: 6)
  2. RNA purification with TRI_ low yield - (reply: 1)
  3. bacterial RNA isolation from PBS buffer water - (reply: 1)
  4. 2 types of cell lines for RNA extraction - (reply: 1)
  5. RNA Gel 3 Sharp bands? - (reply: 1)
  6. Gel Electrophoresis and RNA - (reply: 1)
  7. BLOCK-iT Pol II miR RNAi system: Issues with generating stable knockdown - (reply: 1)
  8. Quantuitative staining of RNA and DNA? - (reply: 4)
  9. How to prepare a "home-made" lysis solution compatible with RNA extracti - (reply: 1)
  10. haeting RNA before electrophoresis - (reply: 1)
  11. Viral RNA in total RNA eluate for transfection - (reply: 1)
  12. Increasing RNA yield from very small tissues - (reply: 3)
  13. tripure rna isolation - (reply: 1)
  14. RNA agarose gel electrophoresis came up empty - (reply: 10)
  15. commercially available miRNA or small RNA? - (reply: 2)
  16. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
  17. Nanodrop question for RNA - (reply: 13)
  18. RNA Precipitation - (reply: 1)
  19. RNA lab - (reply: 1)
  20. What is the protocol to remove RNA contamination (Using RNase) from DNA? - (reply: 8)
  21. What is the RNA:Protein ratio for ONE gene over different RNA levels? - (reply: 1)
  22. Primer Design for RNA probes - (reply: 2)
  23. Real time PCR for degraded RNA - (reply: 1)
  24. RNA contamination in plasmid extracted - (reply: 1)
  25. Any idea of the composition of the RNA Wash Buffer from Zymo Research RNA Clean - (reply: 3)
  26. help with total RNA isolation - (reply: 1)
  27. Protein quantification after TRIzol Extraction - (reply: 2)
  28. Water RNA Contamination? - (reply: 4)
  29. Low RNA quantity after on column DNAse digestion - (reply: 1)
  30. Checking RNA integrity by Denaturing Gel - (reply: 2)
  31. Small yield of RNA after on column DNAs digestion , Why - (reply: 3)
  32. RNA Freeze/Thaw and NanoDrop Concentration - (reply: 1)
  33. software for making RNA secondary structure - (reply: 1)
  34. RNA integrity and RNA fragmentation? - (reply: 1)
  35. RNA isolation, dilution question - (reply: 1)
  36. small rna query - (reply: 1)
  37. rna thawing - (reply: 4)
  38. Cleaning of cuvettes (spectrophotometer) for RNA measurement - (reply: 7)
  39. RNA isolation from whole blood - (reply: 2)
  40. RNA concentration given by Nanodrop - (reply: 9)
  41. RNA yield and quality: Trizol vs. Column based - (reply: 2)
  42. Need help on RNA preparation for RNA sequencing - (reply: 7)
  43. what is the possible mechanism for a downregulation of RNA expression - (reply: 4)
  44. rna extraction protocol - (reply: 3)
  45. Preparation of RNase A From Powder - (reply: 1)
  46. miRNA-mediated competing endogenous RNAs (ceRNAs) networks - (reply: 4)
  47. RNA seq reads - assessing distance from start codon - (reply: 1)
  48. question about RNA concentration for real time PCR - (reply: 1)
  49. RNA extraction from flat worms - (reply: 2)
  50. Comprehensive List of long noncoding RNA(lncRNA) resources - (reply: 2)
  51. Is there any alternative to Formamide in RNA Gel Electrophoresis? - (reply: 1)
  52. RNAse - how to convert ug/ml to U/ml? - (reply: 3)
  53. rna isolation from formalin fixed peg tissue - (reply: 2)
  54. software for drawing small RNA molecules and nucleotides - (reply: 2)
  55. low RNA A260/280 - (reply: 2)
  56. RNA concentration quiries - (reply: 2)
  57. Removing ~200 different transcripts from total RNA pool - (reply: 1)
  58. RNase digestion followed by Radiolabelling iCLIP/CLIP - (reply: 1)
  59. Spleen homogonization for RNA extraction issue - (reply: 1)
  60. cDNA preparation from degraded RNA - (reply: 2)
  61. excess rna sample affect qpcr? - (reply: 2)
  62. How to ship plant tissue for RNA extraction? - (reply: 2)
  63. Concentration of cDNA from unknown quantity of RNA by mistake - (reply: 7)
  64. RNA extraction for RT-PCR - (reply: 3)
  65. Simultaneous RNA and DNA transfection? - (reply: 2)
  66. q value in rna seq - (reply: 1)
  67. Diffuse rRNA bands on RNA denaturing (formaldehyde) gel - (reply: 5)
  68. How to get rid of DNA contamination in RNA prep - (reply: 4)
  69. 100 ng RNA for cDNA synthesis - (reply: 4)
  70. RNA extraction from human jaw bone - (reply: 4)
  71. How to avoid RNA contamination in DNA samples:? - (reply: 1)
  72. RNA contamination in DNA samples - (reply: 2)
  73. Common causes for low RNA A260/230 ratios - (reply: 7)
  74. interpreting RNA agarose gel results? - (reply: 1)
  75. Basic question about qPCR and endogenous RNA control. - (reply: 4)
  76. Biotinylated RNA on Nylon Membrane - (reply: 2)
  77. Nonspecific RNA transcription assay - (reply: 3)
  78. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
  79. RNA concentration suddenly drops - (reply: 3)
  80. RNA concentration to start RT reaction - (reply: 1)
  81. Labeling and tracking RNA - (reply: 3)
  82. Absurdly high A260/280 ratio for RNA - (reply: 2)
  83. accidentally used denatured ethanol for DNA and RNA - what to do? - (reply: 4)
  84. Artificial RNA as normalizer (HK gene) - (reply: 1)
  85. Cannot isolate total RNA using column - (reply: 2)
  86. methods to extract RNA from blood sample? - (reply: 4)
  87. How to prepare serial dilutions of RNA or cDNA - (reply: 3)
  88. RNA extraction by trizol - (reply: 3)
  89. 16S ribosomal RNA sequences (Bacteria and Archaea) - (reply: 4)
  90. agarose gel electrophoresis of RNA - (reply: 1)
  91. Isolation of protein and RNA from RNeasy Minikits? - (reply: 5)
  92. RNA journals - (reply: 4)
  93. RNA storage time - (reply: 1)
  94. TRIZOL trouble - (reply: 2)
  95. RNA extraction - (reply: 3)
  96. micro RNA - (reply: 1)
  97. RNA alcohol/salt precipitation...low temperature - (reply: 1)
  98. long non-coding RNAs biogenesis pathways - (reply: 1)
  99. Extracting total RNA from woody plant roots - (reply: 2)
  100. Problem with precipitating RNA from ethanol solution - (reply: 1)
  101. Buffers RNase decontamination - (reply: 1)
  102. Homozygote DNA but Heterozygote RNA? - (reply: 2)
  103. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
  104. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
  105. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
  106. Long term storage for RNA extracted from pancreas - (reply: 3)
  107. Viral RNA extraction/isolation methods from plant tissue - (reply: 4)
  108. Why RNase H treatment after cDNA synthesis - (reply: 2)
  109. RNA isolation from Sesame seed - (reply: 3)
  110. Homogenize pancreas for RNA extraction - (reply: 3)
  111. Purification of RNA binding proteins by avidin agarose beads - (reply: 2)
  112. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
  113. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
  114. Amount of RNA (ng) for good ct values. - (reply: 3)
  115. polyacrylamide RNA gel - (reply: 4)
  116. Is my RNA degraded? - (reply: 7)
  117. Problems with extraction of RNA from primary culture - (reply: 4)
  118. Sanger Sequencing RNAse Interference? - (reply: 1)
  119. RNA isolation - (reply: 1)
  120. Low yield for small RNA isolation - (reply: 1)
  121. RNA extraction: low RCF by mistake - (reply: 1)
  122. Visualizing RNA on blot - (reply: 1)
  123. Question about RNA and expression - (reply: 5)
  124. RNase inactivation during IHC prior to LCM - (reply: 4)
  125. Protein and RNA interaction - (reply: 1)
  126. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
  127. Large scale Viral RNA purification Kit - (reply: 3)
  128. minimum amount of RNA for RT_PCR - (reply: 1)
  129. Reference RNA for qPCR - (reply: 3)
  130. RNA isolation of murine pancreatic islets - poor ratios - (reply: 3)
  131. Checking pH of RNase Free Solutions - (reply: 1)
  132. Atypical yeild of RNA - (reply: 1)
  133. Problems with RNA extraction from Sorghum anthers/pollen - (reply: 1)
  134. How does DEPC work to inactivate RNases - (reply: 3)
  135. Does Proteinase K digest RNAse in lysis buffer? - (reply: 2)
  136. RNA pellet gone in the wrong concentration of ethanol - (reply: 4)
  137. Problems in RNAi construction by using pUC19 - (reply: 3)
  138. [QUESTION] Is it possible to incorperate Tri Reagent (Trizol) with the Mirvana m - (reply: 3)
  139. Detection of T7 RNA polymerase by WB - (reply: 6)
  140. RNA isolation from small numbers of cells - (reply: 6)
  141. Problems with Electrophoresis of RNA - (reply: 3)
  142. Extraction of RNA from HEPG2 - (reply: 1)
  143. Synthesis of carrier RNA(poly-A) for DNA Extraction - (reply: 2)
  144. 5'RACE of RNA Pol3 transcripts? - (reply: 3)
  145. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  146. RNA integrity - (reply: 5)
  147. Question about PCR - DNA or RNA as template - (reply: 4)
  148. Dyes for RNA in Urea-polyacrylamide gel - (reply: 1)
  149. Incorporating RNAse A into DNA extraction - (reply: 2)
  150. Agarose gel electrophoresis of RNA - (reply: 5)
  151. Recovery of viral RNA - (reply: 3)
  152. Ethanol contamination in RNA - (reply: 11)
  153. blood RNA - (reply: 2)
  154. RNA extraction kits for small samples - (reply: 6)
  155. RNA caculation!! - (reply: 2)
  156. RNA in situ hybridization: pH of AP staining buffer? - (reply: 1)
  157. Issues with Cy5 labeled RNA - (reply: 2)
  158. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  159. How to precipitate RNA from water - (reply: 3)
  160. FFPE tissue and RNA isolation - (reply: 2)
  161. Calculation of RNA - (reply: 2)
  162. My RNA extraction failed (From a newbie ) - (reply: 2)
  163. RNase h electrophoresis - (reply: 1)
  164. Trizol and safety - (reply: 7)
  165. miRNA from plasma_purity problem after RNA isolation - (reply: 1)
  166. RNA degradation problem, only one band on the gel - (reply: 3)
  167. Application of RNase - (reply: 3)
  168. RNAi in combination with adenoviral-mediated over-expression - (reply: 1)
  169. RNA transcription from T3 promoter problem - (reply: 1)
  170. How to purify RNA from a handful number of cells? - (reply: 5)
  171. RNA purification from urine samples - (reply: 2)
  172. Ideas for determining poly(a) status of RNA - (reply: 2)
  173. Is some amount of RNA obligatory for qRT-PCR? - (reply: 5)
  174. Purifying RNA from Solution with RNEasy? - (reply: 2)
  175. Is it possible to extract RNA from Cells frozen in 10% DMSO ? - (reply: 2)
  176. how to design primers for 16sr RNA - (reply: 24)
  177. Isolation of both DNA and RNA from the same plant tissue - (reply: 8)
  178. RNase Band in old plasmid stored at -40 - (reply: 2)
  179. How to lysis cells for an RNA extraction - (reply: 1)
  180. protein solubilization and quantitation after extraction using Trizol - (reply: 2)
  181. Urgent - RNA extraction / RNase contamination ? - (reply: 4)
  182. RNA transfer to nitrocellulose membrane (Northern blot) - (reply: 2)
  183. precipitation of RNA after DNase treatment - (reply: 3)
  184. Can we use denatured DNA standards on RNA formamide gels - (reply: 2)
  185. RNA immunoprecipitation DNase treatment - (reply: 4)
  186. RNA extraction protocol help - (reply: 1)
  187. Higher mRNA level after RNAi? - (reply: 1)
  188. question: RNAi for bacteria? - (reply: 3)
  189. Control for RNA chip experiment - (reply: 1)
  190. High RNase concentration interfering with PCR? - (reply: 8)
  191. RNAi problem - Gene is knocked down, but not protein - (reply: 8)
  192. Mortar and Pestle for RNA Extraction - (reply: 1)
  193. Optimizing Trizol mRNA extraction - (reply: 3)
  194. sample preservation for RNA extraction - (reply: 1)
  195. RNA extraction from blood stain - (reply: 1)
  196. RNA purity - (reply: 3)
  197. RNA Buffer Extraction - (reply: 3)
  198. Viral RNA Extraction from Tissue Culture Supernatant - (reply: 5)
  199. DNA extraction from cerebrospinal fluid using trizol - (reply: 2)
  200. New RNAi video from Nature - (reply: 1)
  201. RNA electrophoresis - (reply: 4)
  202. DNA and RNA extraction - (reply: 6)
  203. RNA extraction of ethanol preserved matherial - (reply: 1)
  204. RNA extraction from paraffin embedded tissue - (reply: 2)
  205. Designing of RNA probe for in situ hybridization - (reply: 1)
  206. RNA extraction from a 1mm brain tissue punch - uncertain about homogenisation - (reply: 2)
  207. total RNA Extraction gel control - (reply: 1)
  208. Isopropanol, Water, and RNA Stability - (reply: 3)
  209. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  210. Concentration of RNA to same amount across different samples - (reply: 5)
  211. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
  212. Dilution of 18s. How much? - (reply: 1)
  213. Difference between <Antisense RNA> & <RNAi>? - (reply: 1)
  214. Extrct RNA from fungus in broth medium - (reply: 2)
  215. Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
  216. RNAse treatment of genomic DNA - concentration and duration? - (reply: 1)
  217. protein did not knock down by shRNA, but RNA did knock down - (reply: 4)
  218. RNA extraction and purity from Drosophila - (reply: 1)
  219. Phenol peak at RNA isolation with Trizol method - (reply: 1)
  220. RNA extraction from human sperm - (reply: 3)
  221. Trizol and Protein extraction - (reply: 2)
  222. RNA extraction and preparation - RTqPCR - (reply: 3)
  223. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
  224. Strange question on TRIzol and RNAlater - (reply: 3)
  225. PAXgene blood RNA tube for RNA extraction - (reply: 1)
  226. RNA labeling with Biotin - (reply: 1)
  227. RT-PCR doesn't work with all RNA used - (reply: 1)
  228. RNA isolation from frozen cells - (reply: 2)
  229. RNA protein complex interaction in 4-12% NU PAGE gradient gel - RNA PROTEIN bEHAVIOR IN GRADIENT GELS (reply: 1)
  230. How many RNA I need for detecting plasma miR expression? - (reply: 1)
  231. To isolate DNA, RNA and Protein from same culture flask for methylation study? - (reply: 2)
  232. No gene amplification, 18s amplification is fine - (reply: 1)
  233. Totai RNA dilution - (reply: 8)
  234. RNA from guinea pig lung=degraded - (reply: 5)
  235. Tissue disruption - for RNA isolation (reply: 2)
  236. cells stably expressing T7 RNA polymerase - (reply: 1)
  237. RNA degradation during electrophoresis?? - (reply: 2)
  238. Total RNA estimation - (reply: 2)
  239. Bioanalyzer and RNA gel - (reply: 2)
  240. Genomic DNA contamination in my RNA sample? - (reply: 8)
  241. could i use sterile deionized water instead of DEPEC water in RNA extraction - (reply: 6)
  242. RNase should be added in which step for gDNA isolation using phenol chloroform m - after lysozyme / preteinase k treatment or after phenol chloroform? (reply: 9)
  243. Checking RNA on gel after DIG labeling - (reply: 1)
  244. Whole blood RNA and DNA extraction help - Expression analysis and epigenetics (reply: 2)
  245. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
  246. is it degraded RNA? - I dont get a clear 3-band after electrophoresis (reply: 6)
  247. TRIZol Reagent additional reagents & protocol clarifications - (reply: 4)
  248. Loss of RNA conc. and quality in Qiagen RNeasy Protocol - (reply: 4)
  249. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
  250. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
  251. template transfer? - how does the rna polymerase switches template? (reply: 1)
  252. RNA precipitation - (reply: 1)
  253. RNA extraction from PBMC layer - how to increase RNA Concentration during RNA extraction from BLOOd (reply: 1)
  254. Nuclear or Cytoplasmic RNA Purification - (reply: 1)
  255. Is DNAse I step necessary after RNA synthesis by in vitro transcription? - (reply: 1)
  256. Remove gDNA from RNA sample - (reply: 3)
  257. Best Total RNA Isolation Kit - For plants, and tiny samples <10mg (reply: 3)
  258. Accidentally add isopropanol into Trizol - (reply: 1)
  259. 18s as endogenous control for Oligo dT RT - Can we use 18s ? (reply: 2)
  260. Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els (reply: 4)
  261. oocye extraction and RNA isolation - (reply: 1)
  262. siRNA screening and RNAse free - (reply: 1)
  263. the red dye in trizol? - (reply: 2)
  264. Sequencing of 18srRNA - (reply: 2)
  265. Can very low RNA concentration cause Ct bias..? - (reply: 3)
  266. RNA carrier when using not so good DNA for bisulfite conversion - (reply: 3)
  267. Unstable A260/A230 in RNA isolation - (reply: 2)
  268. Few basic questions about RNA - (reply: 3)
  269. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
  270. RNA Isolation - (reply: 3)
  271. Manipulating RNA Concentrations/Amounts - (reply: 6)
  272. Why does T7 RNA transcription start with a GGG? - (reply: 5)
  273. Internal Control RNA or DNA - (reply: 4)
  274. RNA extraction - (reply: 3)
  275. can RNA amplify in two-step qPCR? - (reply: 2)
  276. Total RNA only produce 1 band in gel - (reply: 2)
  277. RNAi showing upregulation via QPCR - Help needed (reply: 3)
  278. What causes RNA/DNA to unbind from silica membrane? - (reply: 1)
  279. RNA extraction troubleshooting: low 260/230 - (reply: 1)
  280. RNA in native gel - (reply: 3)
  281. RNA extraction from FTA classic - (reply: 1)
  282. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
  283. TRIzol RNA extraction- What is in the top layer? - (reply: 1)
  284. RNA extraction Question - (reply: 2)
  285. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
  286. Possible solvents for RNA - (reply: 2)
  287. Pooled RNA from healthy controls - delta-delta Ct method (reply: 1)
  288. Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
  289. RNAi is somatic and germline cells - (reply: 3)
  290. How to isolate serum from blood for RNA extraction - (reply: 2)
  291. RNA/cell accumulation of difficult-to-obtain tissue - (reply: 1)
  292. generating cDNA from human brain RNA - (reply: 1)
  293. rna isolation - (reply: 2)
  294. Amplification of region from sperm RNA - sperm RNA stability (reply: 4)
  295. Amplification of 3.4 kb product from RNA for cloning - (reply: 5)
  296. RNA isolation of spleen and thymus: RNA degradation - (reply: 2)
  297. Prepare p:C:I - for RNA extraction (reply: 5)
  298. DNA contamination of RNA samples for qPCR...weird results! - (reply: 3)
  299. Washing RNA pellet - 85% ethanol? - (reply: 2)
  300. Viral RNA is relatively stable than mRNA from cells? - (reply: 1)
  301. Isolating bacterial RNA and protein from the host tissue sample - (reply: 3)
  302. Smears above ribosomal RNA bands - RNA extraction from microalgae (reply: 2)
  303. Trizol Extraction of PBMCs, low 260/230 - (reply: 5)
  304. TRIzol phase extraction with Lock-Phase tubes - Does anyone use Lock-Phase? (reply: 1)
  305. Tissue homogenization buffer to keep viral RNA intact - (reply: 6)
  306. RNA degradation in collagen gels - (reply: 1)
  307. RNA pellet white and low ratio (low purity) - how to improve RNA purity (reply: 5)
  308. nuclear and cytoplasmic RNA - (reply: 2)
  309. rna isolation - (reply: 1)
  310. how does rna precipitate in LiCl solution ? - (reply: 1)
  311. RNA Pol II CHIP protocol help - suggest protocol (reply: 2)
  312. Making solutions for RNA work - (reply: 1)
  313. RNA quality - (reply: 3)
  314. RNA quality - (reply: 3)
  315. How to check RNA quality - (reply: 5)
  316. Cleaning RNase - How do i clean out the RNase from my genomic DNA (reply: 1)
  317. Looking for recommendation for total RNA extraction kit - (reply: 4)
  318. RNA: Is this degratation or something else? - (reply: 1)
  319. rna isolation - (reply: 1)
  320. mRNA preparation from total RNA - a role of poly A in mRNA preparation from total RNA (reply: 2)
  321. Trizol stability - (reply: 1)
  322. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)
  323. RNA fragmentation - (reply: 3)
  324. RNA extraction - how many cells do I need at least?? (reply: 4)
  325. RNA extraction protocol for small amounts of cells - (reply: 2)
  326. cDNA prepared with varying amounts of RNA - is the amount of cDNA produced proportional to the input RNA? (reply: 3)
  327. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
  328. COMPLETE RNA degradation - (reply: 18)
  329. Protein shows KD but RNA level is higher - (reply: 4)
  330. RNA extraction from stained cells - (reply: 2)
  331. low yeild fo RNA after invitro transcription - (reply: 4)
  332. RNA extraction: no pellet... - (reply: 2)
  333. Rnase A for plasmid miniprep - cheaper alternative to qiagen? (reply: 4)
  334. problem with Trizol RNA extraction - (reply: 7)
  335. rnase treatment - (reply: 2)
  336. Trizol isolation of RNA from OCT-embedded frozen tissue - Question (reply: 1)
  337. RNA extraction from trout embryos - (reply: 3)
  338. Which way to clean up TRIzol-extracted RNA? - Ethanol precipitate it first or not? (reply: 2)
  339. RNA extraction expertise needed! - (reply: 2)
  340. Frozen RNA precipitated with ethanol and NaAc! - RNA precipitation (reply: 2)
  341. rna isolation manually - (reply: 2)
  342. Is it sufficient to heat inactivate DNases and RNases? - (reply: 4)
  343. Primers for yeast (18S) - (reply: 1)
  344. problem with Fast Prep Isolation of RNA - problem with Fast Prep Isolation of RNA (reply: 2)
  345. Insect larvae storage for RNA Isolation - looking for a cheap.. easy way.. something innovative!!!!!&# (reply: 4)
  346. RNA solutions - Calculating concentrations (reply: 9)
  347. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
  348. Sample handling for RNA extraction - (reply: 1)
  349. Normalising RNA concentrations - Help (reply: 4)
  350. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
  351. total RNA yield - (reply: 3)
  352. DNase 1 treratment of RNA - (reply: 11)
  353. Concentrating RNA - (reply: 3)
  354. RNA:sharp bends and a smear - (reply: 1)
  355. Measuring DNA, RNA, protein and cell wall synthesis - (reply: 1)
  356. RNA extraction - (reply: 2)
  357. Trizol RNA extraction - (reply: 2)
  358. DNA contamination in RNA samples - (reply: 2)
  359. Extraction of RNA from blood samples - Problems (Trizol method) (reply: 1)
  360. Measurement of RNA concentration after Dnases treatment - (reply: 4)
  361. RNA Probe precipitation - (reply: 9)
  362. RNA immunoprecipitation...HELP! - (reply: 4)
  363. Normalizing RNA concentrations prior to cDNA production - qPCR and making cDNA (reply: 1)
  364. Can't get clean RNA prep with TRIzol - (reply: 2)
  365. RNA from Zebrafish - (reply: 3)
  366. DNA sonication - RNA contamination? - New probe, start all over (reply: 13)
  367. Rnase for flow cytometry - (reply: 1)
  368. Detecting small RNAs via DeepSeq? - (reply: 1)
  369. extraction of viral rna from plant tissue - (reply: 1)
  370. Drying RNA in Speedvac - (reply: 1)
  371. TRIzol v. TRI reagent - (reply: 2)
  372. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
  373. 10X MOPS buffer for RNA gels - It is yellow! (reply: 3)
  374. RNA isolation with peqGold TriFast - (reply: 2)
  375. storing RNA at -20C? - (reply: 4)
  376. RNA Extraction - Extraction of Yeast RNA from mice blood (reply: 1)
  377. How to remove urea from PAA gel? - RNA silver staining (reply: 1)
  378. Large Difference between Ct of 18s rRNA and Target Gene - (reply: 2)
  379. RNA gel to test DIG labeled RNA probe - (reply: 1)
  380. wacky RNA gel - (reply: 1)
  381. RNA marker for isotope labeled - (reply: 2)
  382. Human 18s rRNA - Human 18s rRNA and qRTPCR normalization (reply: 7)
  383. How to remove diphosphate from in vitro transcribed RNA? - (reply: 1)
  384. Reverse-transcription of UV-damaged RNA - (reply: 3)
  385. evolutionarily conserved RNA secondary structures in multiple sequence alignment - how to (reply: 2)
  386. cDNA concentration higher than original RNA - (reply: 4)
  387. 18s control in qPCR - (reply: 2)
  388. DENATURING AGAROSE GEL FOR RNA - (reply: 1)
  389. circular RNA - (reply: 3)
  390. total RNA (for miRNA) extraction from human serum - (reply: 11)
  391. How do i increase my rna yield - How do i increase my rna yield (reply: 3)
  392. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
  393. how to get higher RNA purity? - (reply: 3)
  394. Snap freezing worm samples for RNA prep - is it essential? (reply: 1)
  395. Does RT step smplify RNA - (reply: 2)
  396. gene expression, reverse transcription on total RNA or mRNA? - (reply: 3)
  397. Low RNA yield after extraction by RNA blood mini kit. - (reply: 2)
  398. RNA recovery from Ethanol - (reply: 2)
  399. Question about the control for an RNAi Experiment - (reply: 3)
  400. Plant RNA Isolation Aid - (reply: 3)
  401. freezing during RNA isolation step - (reply: 1)
  402. Low 260/230 ratio in my RNA - (reply: 2)
  403. Low 260/230 ratio in my RNA - (reply: 3)
  404. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
  405. No RNA after Trizol extraction - Lamba max: 230 !!! (reply: 8)
  406. Doubt: Trizol protocol - about certain steps (reply: 9)
  407. Bacteria Lysis Reagent For RNA extraction - Need Expert's Opinion (reply: 12)
  408. PLASMA RNA extraction - (reply: 1)
  409. DNA contamination after RNA extraction, which kit works best? - (reply: 6)
  410. Handling RNA - How to avoid RNase contamination - (reply: 17)
  411. Phenol chloroform ratio during RNA isolation - (reply: 1)
  412. Removal of RNA from DNA sample - (reply: 15)
  413. RNA gel electrohoresis - (reply: 1)
  414. Verified RNAi Sequences - (reply: 4)
  415. DNA and RNA contamination in RT PCR water controls - (reply: 2)
  416. rna from plasmid - (reply: 1)
  417. Best time to isolate RNA for qPCR? - (reply: 1)
  418. Trizol/triReagent Question - (reply: 2)
  419. Variation between spectros - tot RNA concentrations (reply: 1)
  420. Glycogen and speedvac drying - to make sure no RNA loss during speedvac drying (reply: 6)
  421. RNA from MMA embedded calcified bone - (reply: 1)
  422. low od260/230 for rna - (reply: 1)
  423. RNA - bad 230/280 and 260/280 ratio - what to do?? (reply: 3)
  424. RNA gel- nothing to see - (reply: 2)
  425. heating rna before rt reaction? - (reply: 1)
  426. RNA storage - (reply: 3)
  427. RNase treatment before analyzing sonicated chromatin in ChIP - (reply: 2)
  428. automating RNA isolation - Has anyone done automation of yeast RNA isolation at any stage??? (reply: 2)
  429. miRNA and TRIZOL - (reply: 7)
  430. RNA dot blot - (reply: 6)
  431. RNA not visible on gel :( - (reply: 3)
  432. 260/230 ratio - Very low amount of RNA - how to improve 260/230 ratios? (reply: 5)
  433. RNA impossible to dissolve - (reply: 2)
  434. rna storage at -20 - (reply: 2)
  435. Evaluate My Protocol: RNA Extraction w/ Fat. - (reply: 1)
  436. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
  437. si-Fi, Software for RNAi off-target prediction - (reply: 2)
  438. RNA Extraction Mice Adipose Tissue - (reply: 3)
  439. Use of 70% ethanol to remove RNAse from working area - (reply: 2)
  440. Cells to Ct RNA isolation kit (Ambion) - (reply: 1)
  441. RNA elution after stains all staining - (reply: 3)
  442. rna degradation - what goes wrong - (reply: 2)
  443. rna clean up - what is the rna recovery - (reply: 2)
  444. Fast protocol for RNA agarose gel electrophoresis - (reply: 1)
  445. mirVana kit - I can't have small RNA isolated!!! (reply: 5)
  446. RNA extraction 260/230 ratio - (reply: 6)
  447. Experimental Design Problem for RNA FISH - (reply: 1)
  448. concentrating RNA samples - (reply: 7)
  449. RNA, mRNA, sequencing... HELP - (reply: 2)
  450. how to prepare RNAse A from powdered form - (reply: 1)
  451. RNA Purification - After DNase (reply: 3)
  452. Homogenation of Mouse Spinal Cord Tissue for RNA Extraction - Most effective way to do it? (reply: 3)
  453. small RNA Northern - duration of transfer (reply: 1)
  454. rna isolation - (reply: 1)
  455. RNA Isolation - RNA isolation from human cell lines (reply: 2)
  456. Pooling doesn't work - make up rna amount of 10ug (reply: 3)
  457. RNase: RNA ratio - making dsRNA (reply: 1)
  458. How many cells... - ...gives you how much RNA? (reply: 2)
  459. qPCR and quantity of RNA - (reply: 2)
  460. extracting protein from trizol - (reply: 2)
  461. RNA isolation from monocytes - (reply: 1)
  462. Pooling RNA/precipitation/RNA cleanup - advise needed (reply: 1)
  463. RNAi - (reply: 1)
  464. RNA extration from plant - Lower yield... (reply: 1)
  465. How much RNA is needed to see rRNA on a gel? - (reply: 3)
  466. store whole blood with tri-reagent too long caused RNA degradation or poor RNA q - (reply: 1)
  467. T4 RNA ligase - Question about substrate (reply: 13)
  468. how to pool RNA? - (reply: 1)
  469. Is it Possible - Is it possible RNAi cross-reaction with normal cells molecules?? (reply: 1)
  470. pancreas RNA gel run consult - (reply: 1)
  471. Rnase for cell cycle - (reply: 5)
  472. Help: Advices on extracting RNA from pellets? - (reply: 3)
  473. Trizol issues! - Samples are not clean enough after isolation!!! (reply: 2)
  474. RNA quantification in microplate reader - (reply: 3)
  475. tri-reagent rna isolation - (reply: 1)
  476. Trizol RNA/DNA extraction - (reply: 3)
  477. Drosophila Northern Blot and RNA questions - (reply: 2)
  478. RNA isolation for real-time RT-PCR - forgot to inactivate the DNAse (reply: 4)
  479. Harvest intracellular bacterial RNA - (reply: 2)
  480. Plant RNA extraction problem - A cry for help. (reply: 2)
  481. Designing new RNAi - (reply: 2)
  482. RNA reverse transcription riddle - (reply: 6)
  483. Phenol - why different for DNA and RNA extraction? (reply: 9)
  484. PCR, RNA, Northern Blotting???? - (reply: 1)
  485. 2 step RNA isolation - Possible to Isolate RNA in 2 days? (reply: 2)
  486. Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
  487. RNA visualization - (reply: 1)
  488. Genomic DNA contamination of RNA - (reply: 24)
  489. 18S changes with cDNA dilution - (reply: 2)
  490. RNA precipitation problem - (reply: 2)
  491. Challenging question for RNA people - (reply: 1)
  492. RNA contamination in PCR - (reply: 1)
  493. High salt solution to precipitate RNA - (reply: 1)
  494. RNA extraction using TRIzol - (reply: 19)
  495. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
  496. Reverse transcribing organically contaminated RNA - (reply: 2)
  497. Do you lose RNA by quick freezing? - (reply: 5)
  498. No RNA from cell pellet using Qiagen MiniKit!!??!! - (reply: 5)
  499. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  500. RNA extraction for microarray - (reply: 25)
  501. about the total RNA isolation (trizol methods) and further Dnase I - (reply: 3)
  502. RNA cleanup methods (WITHOUT USING KITS!!) - protocol for removing phenol contamination (reply: 5)
  503. Ask for help about the RNA purification protocol by phenol/chlorform methods - Ask for help about the RNA purification protocol by phenol/chlorform m (reply: 2)
  504. RNA extraction - Which is which? (reply: 6)
  505. So confused with RNAi - (reply: 3)
  506. Pancreas RNA extraction - improve protocol needed (reply: 2)
  507. Genomic DNA contamination in RNA sample - (reply: 2)
  508. no 18S band on RNA agarose gel - (reply: 1)
  509. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
  510. multiple bands in my RNA sample? - (reply: 2)
  511. multiple bands in my RNA sample? - (reply: 2)
  512. Principles behind DNA RNA protein isolation and purification - (reply: 2)
  513. RNA isolation form plants - (reply: 4)
  514. 5' end RNA biotin labeling - need protocol (reply: 2)
  515. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
  516. Heating RNA samples for electrophoresis - (reply: 3)
  517. Removing phenol contamination from total RNA - (reply: 9)
  518. how to prepare rnase? - (reply: 6)
  519. Viscous RNA samples - (reply: 1)
  520. Any mRNA amplification kit for microarray? - I have very little amount of RNA but have to submit it for microarray. (reply: 4)
  521. RNAi in Zebrafish - vector for zebrafish knock-down (reply: 4)
  522. RNA isolation -what is happening?- - (reply: 5)
  523. RNA isolation from murine pancreas - (reply: 2)
  524. Qiagen's RNeasy Mini Kit and RNeasy Plus Micro Kit - Having low yields of RNA (reply: 8)
  525. How to prevent RNA degradation in whole blood - (reply: 3)
  526. RNA extraction - (reply: 1)
  527. Freeze thawing RNA - (reply: 4)
  528. RNA appearance on 1.5% gel after DNase I digestion - (reply: 2)
  529. Remove RNA after DNA mini-prep - (reply: 1)
  530. RNA from Sheep and Bovine blood - Isolation of RNA from bovine and ovine whole blood (reply: 3)
  531. Proteinase K to improve RNA extraction yield - (reply: 2)
  532. cloudy phase during RNA extraction with Trizol - (reply: 10)
  533. how to prepare RNase from powder form? - (reply: 9)
  534. RNA isolation following apoptotic cell labeling - (reply: 3)
  535. get rid of RNA from Maxi - (reply: 1)
  536. RNA labeling - (reply: 3)
  537. Best method to concentrate RNA/cDNA? - (reply: 5)
  538. RNA electrophoresis - (reply: 3)
  539. RNA Extraction & EMSA - (reply: 1)
  540. RNA isolation form HUVEC - UGLY RNA!! (reply: 4)
  541. RNA isolation from jellyfish tissue - Anyone has experience? (reply: 1)
  542. RNA Degradation - DNAse I Treatment, 260/230 ratios (reply: 4)
  543. Tissue sonication for RNA extraction - Help :) (reply: 1)
  544. RNA integrity - (reply: 7)
  545. DNA/RNA into nucleotides - (reply: 6)
  546. denature RNA and miRNA Q-PCR - (reply: 1)
  547. Old threads on "siRNA, microRNA and RNAi" are achived here - (reply: 3)
  548. RNA extraction in -80 - (reply: 1)
  549. RNAi in bacteria - Why no finding for gene silencing in bacteria? (reply: 1)
  550. RNase free syringe for Qiagen DNase treatment, could not find any company provid - (reply: 2)