Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
RNA Related Discussions
  1. Preservation of RNA/cDNA samples - (reply: 1)
  2. Using RNA minikit for DNA extraction - (reply: 4)
  3. RNA RNA EMSA - (reply: 6)
  4. The true monomers making RNA and DNA ? - (reply: 1)
  5. Good diluents for RNA - (reply: 1)
  6. bacterial rna isolation - (reply: 1)
  7. RNA later and 2D SDS PAGE - (reply: 3)
  8. Are relaxed plasmids origin of rep sensitive to bp substitution in RNA I/II sequ - (reply: 5)
  9. HTP DNA and RNA extraction options??? - (reply: 2)
  10. Addition of DNase after RNA extraction - (reply: 4)
  11. RNA Conditioning Solution Agilent Pico Chip - (reply: 1)
  12. RNA agarose gel blurry fuzzy. RNase contaminaton? - (reply: 2)
  13. RNA extraction for RNAseq - (reply: 3)
  14. RNA extraction/trizol - (reply: 1)
  15. Starch Contamination during RNA isolation from wheat seeds - (reply: 2)
  16. RNA electrophoresis - (reply: 2)
  17. Inhibition of PCR amplification of bacterial genomic DNA by RNA - (reply: 1)
  18. Any DNA/RNA elements I can include into transgene to delay expression? - (reply: 2)
  19. DNA spin column to get RNA? - (reply: 1)
  20. 260/230 values of RNA and precipitation of RNA - (reply: 2)
  21. Amount of RNA for cDNA - (reply: 4)
  22. RNA extraction - Zymogen kit - (reply: 1)
  23. Repurifying RNA after extraction - No Kits - (reply: 5)
  24. Can restriction enzymes cleave RNA? - (reply: 1)
  25. Isolation of genomic DNA following RNA extraction with Qiazol? - (reply: 1)
  26. method to detect 5ŽOH end of RNA - (reply: 2)
  27. RNA extraction from cervical SWAB help - (reply: 2)
  28. RNA isolation - (reply: 3)
  29. Acceptable RNA A260/280 ratio - (reply: 1)
  30. False positive in empty wells in RNA dot blots - (reply: 2)
  31. RNA extraction from oocytes - (reply: 3)
  32. Low RNA yield in dual RNA/DNA extraction - (reply: 1)
  33. Agarose gel for determining RNA quality (image attached) - (reply: 3)
  34. Visualization of DNA:RNA hybrid on TBE-UREA GEL - (reply: 8)
  35. CanŽt get rid of DNA contamination in RNA - (reply: 2)
  36. RNA gel problem (melted bands) - (reply: 2)
  37. RNA extraction and DNase treatment - (reply: 1)
  38. RNA extraction and DNase treatment - (reply: 2)
  39. Can I snap freeze my tissue (liquid nitrogen) in Lysis buffer for later RNA extr - (reply: 6)
  40. RNA purification with TRI_ low yield - (reply: 1)
  41. bacterial RNA isolation from PBS buffer water - (reply: 1)
  42. polyA RNA staining for dot blot - (reply: 4)
  43. 2 types of cell lines for RNA extraction - (reply: 1)
  44. RNA Gel 3 Sharp bands? - (reply: 1)
  45. Gel Electrophoresis and RNA - (reply: 1)
  46. BLOCK-iT Pol II miR RNAi system: Issues with generating stable knockdown - (reply: 1)
  47. Quantuitative staining of RNA and DNA? - (reply: 4)
  48. How to prepare a "home-made" lysis solution compatible with RNA extracti - (reply: 1)
  49. haeting RNA before electrophoresis - (reply: 1)
  50. Viral RNA in total RNA eluate for transfection - (reply: 1)
  51. Increasing RNA yield from very small tissues - (reply: 3)
  52. tripure rna isolation - (reply: 1)
  53. RNA agarose gel electrophoresis came up empty - (reply: 10)
  54. commercially available miRNA or small RNA? - (reply: 2)
  55. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
  56. Nanodrop question for RNA - (reply: 13)
  57. RNA Precipitation - (reply: 1)
  58. RNA lab - (reply: 1)
  59. What is the protocol to remove RNA contamination (Using RNase) from DNA? - (reply: 8)
  60. What is the RNA:Protein ratio for ONE gene over different RNA levels? - (reply: 1)
  61. Primer Design for RNA probes - (reply: 2)
  62. Real time PCR for degraded RNA - (reply: 1)
  63. RNA contamination in plasmid extracted - (reply: 1)
  64. Any idea of the composition of the RNA Wash Buffer from Zymo Research RNA Clean - (reply: 3)
  65. help with total RNA isolation - (reply: 1)
  66. Protein quantification after TRIzol Extraction - (reply: 2)
  67. Water RNA Contamination? - (reply: 4)
  68. Low RNA quantity after on column DNAse digestion - (reply: 1)
  69. Checking RNA integrity by Denaturing Gel - (reply: 2)
  70. Small yield of RNA after on column DNAs digestion , Why - (reply: 3)
  71. RNA Freeze/Thaw and NanoDrop Concentration - (reply: 1)
  72. software for making RNA secondary structure - (reply: 1)
  73. RNA integrity and RNA fragmentation? - (reply: 1)
  74. RNA isolation, dilution question - (reply: 1)
  75. small rna query - (reply: 1)
  76. rna thawing - (reply: 4)
  77. Cleaning of cuvettes (spectrophotometer) for RNA measurement - (reply: 7)
  78. RNA isolation from whole blood - (reply: 2)
  79. RNA concentration given by Nanodrop - (reply: 9)
  80. RNA yield and quality: Trizol vs. Column based - (reply: 2)
  81. Need help on RNA preparation for RNA sequencing - (reply: 7)
  82. what is the possible mechanism for a downregulation of RNA expression - (reply: 4)
  83. rna extraction protocol - (reply: 3)
  84. Preparation of RNase A From Powder - (reply: 1)
  85. miRNA-mediated competing endogenous RNAs (ceRNAs) networks - (reply: 4)
  86. RNA seq reads - assessing distance from start codon - (reply: 1)
  87. question about RNA concentration for real time PCR - (reply: 1)
  88. RNA extraction from flat worms - (reply: 2)
  89. Comprehensive List of long noncoding RNA(lncRNA) resources - (reply: 2)
  90. Is there any alternative to Formamide in RNA Gel Electrophoresis? - (reply: 1)
  91. RNAse - how to convert ug/ml to U/ml? - (reply: 3)
  92. rna isolation from formalin fixed peg tissue - (reply: 2)
  93. software for drawing small RNA molecules and nucleotides - (reply: 2)
  94. low RNA A260/280 - (reply: 2)
  95. RNA concentration quiries - (reply: 2)
  96. Removing ~200 different transcripts from total RNA pool - (reply: 1)
  97. RNase digestion followed by Radiolabelling iCLIP/CLIP - (reply: 1)
  98. Spleen homogonization for RNA extraction issue - (reply: 1)
  99. cDNA preparation from degraded RNA - (reply: 2)
  100. excess rna sample affect qpcr? - (reply: 2)
  101. How to ship plant tissue for RNA extraction? - (reply: 2)
  102. Concentration of cDNA from unknown quantity of RNA by mistake - (reply: 7)
  103. RNA extraction for RT-PCR - (reply: 3)
  104. Simultaneous RNA and DNA transfection? - (reply: 2)
  105. q value in rna seq - (reply: 1)
  106. Diffuse rRNA bands on RNA denaturing (formaldehyde) gel - (reply: 5)
  107. How to get rid of DNA contamination in RNA prep - (reply: 4)
  108. 100 ng RNA for cDNA synthesis - (reply: 4)
  109. RNA extraction from human jaw bone - (reply: 4)
  110. How to avoid RNA contamination in DNA samples:? - (reply: 1)
  111. RNA contamination in DNA samples - (reply: 2)
  112. Common causes for low RNA A260/230 ratios - (reply: 7)
  113. interpreting RNA agarose gel results? - (reply: 1)
  114. Basic question about qPCR and endogenous RNA control. - (reply: 4)
  115. Biotinylated RNA on Nylon Membrane - (reply: 2)
  116. Nonspecific RNA transcription assay - (reply: 3)
  117. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
  118. RNA concentration suddenly drops - (reply: 3)
  119. RNA concentration to start RT reaction - (reply: 1)
  120. Labeling and tracking RNA - (reply: 3)
  121. Absurdly high A260/280 ratio for RNA - (reply: 2)
  122. accidentally used denatured ethanol for DNA and RNA - what to do? - (reply: 4)
  123. Artificial RNA as normalizer (HK gene) - (reply: 1)
  124. Cannot isolate total RNA using column - (reply: 2)
  125. methods to extract RNA from blood sample? - (reply: 4)
  126. How to prepare serial dilutions of RNA or cDNA - (reply: 3)
  127. RNA extraction by trizol - (reply: 3)
  128. 16S ribosomal RNA sequences (Bacteria and Archaea) - (reply: 4)
  129. agarose gel electrophoresis of RNA - (reply: 1)
  130. Isolation of protein and RNA from RNeasy Minikits? - (reply: 5)
  131. RNA journals - (reply: 4)
  132. RNA storage time - (reply: 1)
  133. TRIZOL trouble - (reply: 2)
  134. RNA extraction - (reply: 3)
  135. micro RNA - (reply: 1)
  136. RNA alcohol/salt precipitation...low temperature - (reply: 1)
  137. long non-coding RNAs biogenesis pathways - (reply: 1)
  138. Extracting total RNA from woody plant roots - (reply: 2)
  139. Problem with precipitating RNA from ethanol solution - (reply: 1)
  140. Buffers RNase decontamination - (reply: 1)
  141. Homozygote DNA but Heterozygote RNA? - (reply: 2)
  142. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
  143. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
  144. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
  145. Long term storage for RNA extracted from pancreas - (reply: 3)
  146. Viral RNA extraction/isolation methods from plant tissue - (reply: 4)
  147. Why RNase H treatment after cDNA synthesis - (reply: 2)
  148. RNA isolation from Sesame seed - (reply: 3)
  149. Homogenize pancreas for RNA extraction - (reply: 3)
  150. Purification of RNA binding proteins by avidin agarose beads - (reply: 2)
  151. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
  152. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
  153. Amount of RNA (ng) for good ct values. - (reply: 3)
  154. polyacrylamide RNA gel - (reply: 4)
  155. Is my RNA degraded? - (reply: 7)
  156. Problems with extraction of RNA from primary culture - (reply: 4)
  157. Sanger Sequencing RNAse Interference? - (reply: 1)
  158. RNA isolation - (reply: 1)
  159. Low yield for small RNA isolation - (reply: 1)
  160. RNA extraction: low RCF by mistake - (reply: 1)
  161. Visualizing RNA on blot - (reply: 1)
  162. Question about RNA and expression - (reply: 5)
  163. RNase inactivation during IHC prior to LCM - (reply: 4)
  164. Protein and RNA interaction - (reply: 1)
  165. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
  166. Large scale Viral RNA purification Kit - (reply: 3)
  167. minimum amount of RNA for RT_PCR - (reply: 1)
  168. Reference RNA for qPCR - (reply: 3)
  169. RNA isolation of murine pancreatic islets - poor ratios - (reply: 3)
  170. Checking pH of RNase Free Solutions - (reply: 1)
  171. Atypical yeild of RNA - (reply: 1)
  172. Problems with RNA extraction from Sorghum anthers/pollen - (reply: 1)
  173. How does DEPC work to inactivate RNases - (reply: 3)
  174. Does Proteinase K digest RNAse in lysis buffer? - (reply: 2)
  175. RNA pellet gone in the wrong concentration of ethanol - (reply: 4)
  176. Problems in RNAi construction by using pUC19 - (reply: 3)
  177. [QUESTION] Is it possible to incorperate Tri Reagent (Trizol) with the Mirvana m - (reply: 3)
  178. Detection of T7 RNA polymerase by WB - (reply: 6)
  179. RNA isolation from small numbers of cells - (reply: 6)
  180. Problems with Electrophoresis of RNA - (reply: 3)
  181. Extraction of RNA from HEPG2 - (reply: 1)
  182. Synthesis of carrier RNA(poly-A) for DNA Extraction - (reply: 2)
  183. 5'RACE of RNA Pol3 transcripts? - (reply: 3)
  184. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  185. RNA integrity - (reply: 5)
  186. Question about PCR - DNA or RNA as template - (reply: 4)
  187. Dyes for RNA in Urea-polyacrylamide gel - (reply: 1)
  188. Incorporating RNAse A into DNA extraction - (reply: 2)
  189. Agarose gel electrophoresis of RNA - (reply: 5)
  190. Recovery of viral RNA - (reply: 3)
  191. Ethanol contamination in RNA - (reply: 11)
  192. blood RNA - (reply: 2)
  193. RNA extraction kits for small samples - (reply: 6)
  194. RNA caculation!! - (reply: 2)
  195. RNA in situ hybridization: pH of AP staining buffer? - (reply: 1)
  196. Issues with Cy5 labeled RNA - (reply: 2)
  197. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  198. How to precipitate RNA from water - (reply: 3)
  199. FFPE tissue and RNA isolation - (reply: 2)
  200. Calculation of RNA - (reply: 2)
  201. My RNA extraction failed (From a newbie ) - (reply: 2)
  202. RNase h electrophoresis - (reply: 1)
  203. Trizol and safety - (reply: 7)
  204. miRNA from plasma_purity problem after RNA isolation - (reply: 1)
  205. RNA degradation problem, only one band on the gel - (reply: 3)
  206. Application of RNase - (reply: 3)
  207. RNAi in combination with adenoviral-mediated over-expression - (reply: 1)
  208. RNA transcription from T3 promoter problem - (reply: 1)
  209. How to purify RNA from a handful number of cells? - (reply: 5)
  210. RNA purification from urine samples - (reply: 2)
  211. Ideas for determining poly(a) status of RNA - (reply: 2)
  212. Is some amount of RNA obligatory for qRT-PCR? - (reply: 5)
  213. Purifying RNA from Solution with RNEasy? - (reply: 2)
  214. Is it possible to extract RNA from Cells frozen in 10% DMSO ? - (reply: 2)
  215. how to design primers for 16sr RNA - (reply: 24)
  216. Isolation of both DNA and RNA from the same plant tissue - (reply: 8)
  217. RNase Band in old plasmid stored at -40 - (reply: 2)
  218. How to lysis cells for an RNA extraction - (reply: 1)
  219. protein solubilization and quantitation after extraction using Trizol - (reply: 2)
  220. Urgent - RNA extraction / RNase contamination ? - (reply: 4)
  221. RNA transfer to nitrocellulose membrane (Northern blot) - (reply: 2)
  222. precipitation of RNA after DNase treatment - (reply: 3)
  223. Can we use denatured DNA standards on RNA formamide gels - (reply: 2)
  224. RNA immunoprecipitation DNase treatment - (reply: 4)
  225. RNA extraction protocol help - (reply: 1)
  226. Higher mRNA level after RNAi? - (reply: 1)
  227. question: RNAi for bacteria? - (reply: 3)
  228. Control for RNA chip experiment - (reply: 1)
  229. High RNase concentration interfering with PCR? - (reply: 8)
  230. RNAi problem - Gene is knocked down, but not protein - (reply: 9)
  231. Mortar and Pestle for RNA Extraction - (reply: 1)
  232. Optimizing Trizol mRNA extraction - (reply: 3)
  233. sample preservation for RNA extraction - (reply: 1)
  234. RNA extraction from blood stain - (reply: 1)
  235. RNA purity - (reply: 3)
  236. RNA Buffer Extraction - (reply: 3)
  237. Viral RNA Extraction from Tissue Culture Supernatant - (reply: 5)
  238. DNA extraction from cerebrospinal fluid using trizol - (reply: 2)
  239. New RNAi video from Nature - (reply: 1)
  240. RNA electrophoresis - (reply: 4)
  241. DNA and RNA extraction - (reply: 6)
  242. RNA extraction of ethanol preserved matherial - (reply: 1)
  243. RNA extraction from paraffin embedded tissue - (reply: 2)
  244. Designing of RNA probe for in situ hybridization - (reply: 1)
  245. RNA extraction from a 1mm brain tissue punch - uncertain about homogenisation - (reply: 2)
  246. total RNA Extraction gel control - (reply: 1)
  247. Isopropanol, Water, and RNA Stability - (reply: 3)
  248. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  249. Concentration of RNA to same amount across different samples - (reply: 5)
  250. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
  251. Dilution of 18s. How much? - (reply: 1)
  252. Difference between <Antisense RNA> & <RNAi>? - (reply: 1)
  253. Extrct RNA from fungus in broth medium - (reply: 2)
  254. Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
  255. RNAse treatment of genomic DNA - concentration and duration? - (reply: 1)
  256. protein did not knock down by shRNA, but RNA did knock down - (reply: 4)
  257. RNA extraction and purity from Drosophila - (reply: 1)
  258. Phenol peak at RNA isolation with Trizol method - (reply: 1)
  259. RNA extraction from human sperm - (reply: 3)
  260. Trizol and Protein extraction - (reply: 2)
  261. RNA extraction and preparation - RTqPCR - (reply: 3)
  262. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
  263. Strange question on TRIzol and RNAlater - (reply: 3)
  264. PAXgene blood RNA tube for RNA extraction - (reply: 1)
  265. RNA labeling with Biotin - (reply: 1)
  266. RT-PCR doesn't work with all RNA used - (reply: 1)
  267. RNA isolation from frozen cells - (reply: 2)
  268. RNA protein complex interaction in 4-12% NU PAGE gradient gel - RNA PROTEIN bEHAVIOR IN GRADIENT GELS (reply: 1)
  269. How many RNA I need for detecting plasma miR expression? - (reply: 1)
  270. To isolate DNA, RNA and Protein from same culture flask for methylation study? - (reply: 2)
  271. No gene amplification, 18s amplification is fine - (reply: 1)
  272. Totai RNA dilution - (reply: 8)
  273. RNA from guinea pig lung=degraded - (reply: 5)
  274. Tissue disruption - for RNA isolation (reply: 2)
  275. cells stably expressing T7 RNA polymerase - (reply: 1)
  276. RNA degradation during electrophoresis?? - (reply: 2)
  277. Total RNA estimation - (reply: 2)
  278. Bioanalyzer and RNA gel - (reply: 2)
  279. Genomic DNA contamination in my RNA sample? - (reply: 8)
  280. could i use sterile deionized water instead of DEPEC water in RNA extraction - (reply: 6)
  281. RNase should be added in which step for gDNA isolation using phenol chloroform m - after lysozyme / preteinase k treatment or after phenol chloroform? (reply: 9)
  282. Checking RNA on gel after DIG labeling - (reply: 1)
  283. Whole blood RNA and DNA extraction help - Expression analysis and epigenetics (reply: 2)
  284. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
  285. is it degraded RNA? - I dont get a clear 3-band after electrophoresis (reply: 6)
  286. TRIZol Reagent additional reagents & protocol clarifications - (reply: 4)
  287. Loss of RNA conc. and quality in Qiagen RNeasy Protocol - (reply: 4)
  288. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
  289. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
  290. template transfer? - how does the rna polymerase switches template? (reply: 1)
  291. RNA precipitation - (reply: 1)
  292. RNA extraction from PBMC layer - how to increase RNA Concentration during RNA extraction from BLOOd (reply: 1)
  293. Nuclear or Cytoplasmic RNA Purification - (reply: 1)
  294. Is DNAse I step necessary after RNA synthesis by in vitro transcription? - (reply: 1)
  295. Remove gDNA from RNA sample - (reply: 3)
  296. Best Total RNA Isolation Kit - For plants, and tiny samples <10mg (reply: 3)
  297. Accidentally add isopropanol into Trizol - (reply: 1)
  298. 18s as endogenous control for Oligo dT RT - Can we use 18s ? (reply: 2)
  299. Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els (reply: 4)
  300. oocye extraction and RNA isolation - (reply: 1)
  301. siRNA screening and RNAse free - (reply: 1)
  302. the red dye in trizol? - (reply: 2)
  303. Sequencing of 18srRNA - (reply: 2)
  304. Can very low RNA concentration cause Ct bias..? - (reply: 3)
  305. RNA carrier when using not so good DNA for bisulfite conversion - (reply: 3)
  306. Unstable A260/A230 in RNA isolation - (reply: 2)
  307. Few basic questions about RNA - (reply: 3)
  308. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
  309. RNA Isolation - (reply: 3)
  310. Manipulating RNA Concentrations/Amounts - (reply: 6)
  311. Why does T7 RNA transcription start with a GGG? - (reply: 5)
  312. Internal Control RNA or DNA - (reply: 4)
  313. RNA extraction - (reply: 3)
  314. can RNA amplify in two-step qPCR? - (reply: 2)
  315. Total RNA only produce 1 band in gel - (reply: 2)
  316. RNAi showing upregulation via QPCR - Help needed (reply: 3)
  317. What causes RNA/DNA to unbind from silica membrane? - (reply: 1)
  318. RNA extraction troubleshooting: low 260/230 - (reply: 1)
  319. RNA in native gel - (reply: 3)
  320. RNA extraction from FTA classic - (reply: 1)
  321. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
  322. TRIzol RNA extraction- What is in the top layer? - (reply: 1)
  323. RNA extraction Question - (reply: 2)
  324. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
  325. Possible solvents for RNA - (reply: 2)
  326. Pooled RNA from healthy controls - delta-delta Ct method (reply: 1)
  327. Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
  328. RNAi is somatic and germline cells - (reply: 3)
  329. How to isolate serum from blood for RNA extraction - (reply: 2)
  330. RNA/cell accumulation of difficult-to-obtain tissue - (reply: 1)
  331. generating cDNA from human brain RNA - (reply: 1)
  332. rna isolation - (reply: 2)
  333. Amplification of region from sperm RNA - sperm RNA stability (reply: 4)
  334. Amplification of 3.4 kb product from RNA for cloning - (reply: 5)
  335. RNA isolation of spleen and thymus: RNA degradation - (reply: 2)
  336. Prepare p:C:I - for RNA extraction (reply: 5)
  337. DNA contamination of RNA samples for qPCR...weird results! - (reply: 3)
  338. Washing RNA pellet - 85% ethanol? - (reply: 2)
  339. Viral RNA is relatively stable than mRNA from cells? - (reply: 1)
  340. Isolating bacterial RNA and protein from the host tissue sample - (reply: 3)
  341. Smears above ribosomal RNA bands - RNA extraction from microalgae (reply: 2)
  342. Trizol Extraction of PBMCs, low 260/230 - (reply: 5)
  343. TRIzol phase extraction with Lock-Phase tubes - Does anyone use Lock-Phase? (reply: 1)
  344. Tissue homogenization buffer to keep viral RNA intact - (reply: 6)
  345. RNA degradation in collagen gels - (reply: 1)
  346. RNA pellet white and low ratio (low purity) - how to improve RNA purity (reply: 5)
  347. nuclear and cytoplasmic RNA - (reply: 2)
  348. rna isolation - (reply: 1)
  349. how does rna precipitate in LiCl solution ? - (reply: 1)
  350. RNA Pol II CHIP protocol help - suggest protocol (reply: 2)
  351. Making solutions for RNA work - (reply: 1)
  352. RNA quality - (reply: 3)
  353. RNA quality - (reply: 3)
  354. How to check RNA quality - (reply: 5)
  355. Cleaning RNase - How do i clean out the RNase from my genomic DNA (reply: 1)
  356. Looking for recommendation for total RNA extraction kit - (reply: 4)
  357. RNA: Is this degratation or something else? - (reply: 1)
  358. rna isolation - (reply: 1)
  359. mRNA preparation from total RNA - a role of poly A in mRNA preparation from total RNA (reply: 2)
  360. Trizol stability - (reply: 1)
  361. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)
  362. RNA fragmentation - (reply: 3)
  363. RNA extraction - how many cells do I need at least?? (reply: 4)
  364. RNA extraction protocol for small amounts of cells - (reply: 2)
  365. cDNA prepared with varying amounts of RNA - is the amount of cDNA produced proportional to the input RNA? (reply: 3)
  366. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
  367. COMPLETE RNA degradation - (reply: 18)
  368. Protein shows KD but RNA level is higher - (reply: 4)
  369. RNA extraction from stained cells - (reply: 2)
  370. low yeild fo RNA after invitro transcription - (reply: 4)
  371. RNA extraction: no pellet... - (reply: 2)
  372. Rnase A for plasmid miniprep - cheaper alternative to qiagen? (reply: 4)
  373. problem with Trizol RNA extraction - (reply: 7)
  374. rnase treatment - (reply: 2)
  375. Trizol isolation of RNA from OCT-embedded frozen tissue - Question (reply: 1)
  376. RNA extraction from trout embryos - (reply: 3)
  377. Which way to clean up TRIzol-extracted RNA? - Ethanol precipitate it first or not? (reply: 2)
  378. RNA extraction expertise needed! - (reply: 2)
  379. Frozen RNA precipitated with ethanol and NaAc! - RNA precipitation (reply: 2)
  380. rna isolation manually - (reply: 2)
  381. Is it sufficient to heat inactivate DNases and RNases? - (reply: 4)
  382. Primers for yeast (18S) - (reply: 1)
  383. problem with Fast Prep Isolation of RNA - problem with Fast Prep Isolation of RNA (reply: 2)
  384. Insect larvae storage for RNA Isolation - looking for a cheap.. easy way.. something innovative!!!!!&# (reply: 4)
  385. RNA solutions - Calculating concentrations (reply: 9)
  386. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
  387. Sample handling for RNA extraction - (reply: 1)
  388. Normalising RNA concentrations - Help (reply: 4)
  389. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
  390. total RNA yield - (reply: 3)
  391. DNase 1 treratment of RNA - (reply: 11)
  392. Concentrating RNA - (reply: 3)
  393. RNA:sharp bends and a smear - (reply: 1)
  394. Measuring DNA, RNA, protein and cell wall synthesis - (reply: 1)
  395. RNA extraction - (reply: 2)
  396. Trizol RNA extraction - (reply: 2)
  397. DNA contamination in RNA samples - (reply: 2)
  398. Extraction of RNA from blood samples - Problems (Trizol method) (reply: 1)
  399. Measurement of RNA concentration after Dnases treatment - (reply: 4)
  400. RNA Probe precipitation - (reply: 9)
  401. RNA immunoprecipitation...HELP! - (reply: 4)
  402. Normalizing RNA concentrations prior to cDNA production - qPCR and making cDNA (reply: 1)
  403. Can't get clean RNA prep with TRIzol - (reply: 2)
  404. RNA from Zebrafish - (reply: 3)
  405. DNA sonication - RNA contamination? - New probe, start all over (reply: 13)
  406. Rnase for flow cytometry - (reply: 1)
  407. Detecting small RNAs via DeepSeq? - (reply: 1)
  408. extraction of viral rna from plant tissue - (reply: 1)
  409. Drying RNA in Speedvac - (reply: 1)
  410. TRIzol v. TRI reagent - (reply: 2)
  411. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
  412. 10X MOPS buffer for RNA gels - It is yellow! (reply: 3)
  413. RNA isolation with peqGold TriFast - (reply: 2)
  414. storing RNA at -20C? - (reply: 4)
  415. RNA Extraction - Extraction of Yeast RNA from mice blood (reply: 1)
  416. How to remove urea from PAA gel? - RNA silver staining (reply: 1)
  417. Large Difference between Ct of 18s rRNA and Target Gene - (reply: 2)
  418. RNA gel to test DIG labeled RNA probe - (reply: 1)
  419. wacky RNA gel - (reply: 1)
  420. DNA/RNA extraction from sputum - (reply: 3)
  421. RNA marker for isotope labeled - (reply: 2)
  422. Human 18s rRNA - Human 18s rRNA and qRTPCR normalization (reply: 7)
  423. How to remove diphosphate from in vitro transcribed RNA? - (reply: 1)
  424. Reverse-transcription of UV-damaged RNA - (reply: 3)
  425. evolutionarily conserved RNA secondary structures in multiple sequence alignment - how to (reply: 2)
  426. cDNA concentration higher than original RNA - (reply: 4)
  427. 18s control in qPCR - (reply: 2)
  428. DENATURING AGAROSE GEL FOR RNA - (reply: 1)
  429. circular RNA - (reply: 3)
  430. total RNA (for miRNA) extraction from human serum - (reply: 11)
  431. How do i increase my rna yield - How do i increase my rna yield (reply: 3)
  432. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
  433. how to get higher RNA purity? - (reply: 3)
  434. Snap freezing worm samples for RNA prep - is it essential? (reply: 1)
  435. Does RT step smplify RNA - (reply: 2)
  436. gene expression, reverse transcription on total RNA or mRNA? - (reply: 3)
  437. Low RNA yield after extraction by RNA blood mini kit. - (reply: 2)
  438. RNA recovery from Ethanol - (reply: 2)
  439. Question about the control for an RNAi Experiment - (reply: 3)
  440. Plant RNA Isolation Aid - (reply: 3)
  441. freezing during RNA isolation step - (reply: 1)
  442. Low 260/230 ratio in my RNA - (reply: 2)
  443. Low 260/230 ratio in my RNA - (reply: 3)
  444. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
  445. No RNA after Trizol extraction - Lamba max: 230 !!! (reply: 8)
  446. Doubt: Trizol protocol - about certain steps (reply: 9)
  447. Bacteria Lysis Reagent For RNA extraction - Need Expert's Opinion (reply: 12)
  448. PLASMA RNA extraction - (reply: 1)
  449. DNA contamination after RNA extraction, which kit works best? - (reply: 6)
  450. Handling RNA - How to avoid RNase contamination - (reply: 17)
  451. Phenol chloroform ratio during RNA isolation - (reply: 1)
  452. Removal of RNA from DNA sample - (reply: 15)
  453. RNA gel electrohoresis - (reply: 1)
  454. Verified RNAi Sequences - (reply: 4)
  455. DNA and RNA contamination in RT PCR water controls - (reply: 2)
  456. rna from plasmid - (reply: 1)
  457. Best time to isolate RNA for qPCR? - (reply: 1)
  458. Trizol/triReagent Question - (reply: 2)
  459. Variation between spectros - tot RNA concentrations (reply: 1)
  460. Glycogen and speedvac drying - to make sure no RNA loss during speedvac drying (reply: 6)
  461. RNA from MMA embedded calcified bone - (reply: 1)
  462. low od260/230 for rna - (reply: 1)
  463. RNA - bad 230/280 and 260/280 ratio - what to do?? (reply: 3)
  464. RNA gel- nothing to see - (reply: 2)
  465. heating rna before rt reaction? - (reply: 1)
  466. RNA storage - (reply: 3)
  467. RNase treatment before analyzing sonicated chromatin in ChIP - (reply: 2)
  468. automating RNA isolation - Has anyone done automation of yeast RNA isolation at any stage??? (reply: 2)
  469. miRNA and TRIZOL - (reply: 7)
  470. RNA dot blot - (reply: 6)
  471. RNA not visible on gel :( - (reply: 3)
  472. 260/230 ratio - Very low amount of RNA - how to improve 260/230 ratios? (reply: 5)
  473. RNA impossible to dissolve - (reply: 2)
  474. rna storage at -20 - (reply: 2)
  475. Evaluate My Protocol: RNA Extraction w/ Fat. - (reply: 1)
  476. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
  477. si-Fi, Software for RNAi off-target prediction - (reply: 2)
  478. RNA Extraction Mice Adipose Tissue - (reply: 3)
  479. Use of 70% ethanol to remove RNAse from working area - (reply: 2)
  480. Cells to Ct RNA isolation kit (Ambion) - (reply: 1)
  481. RNA elution after stains all staining - (reply: 3)
  482. rna degradation - what goes wrong - (reply: 2)
  483. rna clean up - what is the rna recovery - (reply: 2)
  484. Fast protocol for RNA agarose gel electrophoresis - (reply: 1)
  485. mirVana kit - I can't have small RNA isolated!!! (reply: 5)
  486. RNA extraction 260/230 ratio - (reply: 6)
  487. Experimental Design Problem for RNA FISH - (reply: 1)
  488. concentrating RNA samples - (reply: 7)
  489. RNA, mRNA, sequencing... HELP - (reply: 2)
  490. how to prepare RNAse A from powdered form - (reply: 1)
  491. RNA Purification - After DNase (reply: 3)
  492. Homogenation of Mouse Spinal Cord Tissue for RNA Extraction - Most effective way to do it? (reply: 3)
  493. small RNA Northern - duration of transfer (reply: 1)
  494. rna isolation - (reply: 1)
  495. RNA Isolation - RNA isolation from human cell lines (reply: 2)
  496. Pooling doesn't work - make up rna amount of 10ug (reply: 3)
  497. RNase: RNA ratio - making dsRNA (reply: 1)
  498. How many cells... - ...gives you how much RNA? (reply: 2)
  499. qPCR and quantity of RNA - (reply: 2)
  500. extracting protein from trizol - (reply: 2)
  501. RNA isolation from monocytes - (reply: 1)
  502. Pooling RNA/precipitation/RNA cleanup - advise needed (reply: 1)
  503. RNAi - (reply: 1)
  504. RNA extration from plant - Lower yield... (reply: 1)
  505. How much RNA is needed to see rRNA on a gel? - (reply: 3)
  506. store whole blood with tri-reagent too long caused RNA degradation or poor RNA q - (reply: 1)
  507. T4 RNA ligase - Question about substrate (reply: 13)
  508. how to pool RNA? - (reply: 1)
  509. Is it Possible - Is it possible RNAi cross-reaction with normal cells molecules?? (reply: 1)
  510. pancreas RNA gel run consult - (reply: 1)
  511. Rnase for cell cycle - (reply: 5)
  512. Help: Advices on extracting RNA from pellets? - (reply: 3)
  513. Trizol issues! - Samples are not clean enough after isolation!!! (reply: 2)
  514. RNA quantification in microplate reader - (reply: 3)
  515. tri-reagent rna isolation - (reply: 1)
  516. Trizol RNA/DNA extraction - (reply: 3)
  517. Drosophila Northern Blot and RNA questions - (reply: 2)
  518. RNA isolation for real-time RT-PCR - forgot to inactivate the DNAse (reply: 4)
  519. Harvest intracellular bacterial RNA - (reply: 2)
  520. Plant RNA extraction problem - A cry for help. (reply: 2)
  521. Designing new RNAi - (reply: 2)
  522. RNA reverse transcription riddle - (reply: 6)
  523. Phenol - why different for DNA and RNA extraction? (reply: 9)
  524. PCR, RNA, Northern Blotting???? - (reply: 1)
  525. 2 step RNA isolation - Possible to Isolate RNA in 2 days? (reply: 2)
  526. Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
  527. RNA visualization - (reply: 1)
  528. Genomic DNA contamination of RNA - (reply: 24)
  529. 18S changes with cDNA dilution - (reply: 2)
  530. RNA precipitation problem - (reply: 2)
  531. Challenging question for RNA people - (reply: 1)
  532. RNA contamination in PCR - (reply: 1)
  533. High salt solution to precipitate RNA - (reply: 1)
  534. RNA extraction using TRIzol - (reply: 19)
  535. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
  536. Reverse transcribing organically contaminated RNA - (reply: 2)
  537. Do you lose RNA by quick freezing? - (reply: 5)
  538. No RNA from cell pellet using Qiagen MiniKit!!??!! - (reply: 5)
  539. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  540. RNA extraction for microarray - (reply: 25)
  541. about the total RNA isolation (trizol methods) and further Dnase I - (reply: 3)
  542. RNA cleanup methods (WITHOUT USING KITS!!) - protocol for removing phenol contamination (reply: 5)
  543. Ask for help about the RNA purification protocol by phenol/chlorform methods - Ask for help about the RNA purification protocol by phenol/chlorform m (reply: 2)
  544. RNA extraction - Which is which? (reply: 6)
  545. So confused with RNAi - (reply: 3)
  546. Pancreas RNA extraction - improve protocol needed (reply: 2)
  547. Genomic DNA contamination in RNA sample - (reply: 2)
  548. no 18S band on RNA agarose gel - (reply: 1)
  549. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
  550. multiple bands in my RNA sample? - (reply: 2)
  551. multiple bands in my RNA sample? - (reply: 2)
  552. Principles behind DNA RNA protein isolation and purification - (reply: 2)
  553. RNA isolation form plants - (reply: 4)
  554. 5' end RNA biotin labeling - need protocol (reply: 2)
  555. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
  556. Heating RNA samples for electrophoresis - (reply: 3)
  557. Removing phenol contamination from total RNA - (reply: 9)
  558. how to prepare rnase? - (reply: 6)
  559. Viscous RNA samples - (reply: 1)
  560. Any mRNA amplification kit for microarray? - I have very little amount of RNA but have to submit it for microarray. (reply: 4)
  561. RNAi in Zebrafish - vector for zebrafish knock-down (reply: 4)
  562. RNA isolation -what is happening?- - (reply: 5)
  563. RNA isolation from murine pancreas - (reply: 2)
  564. Qiagen's RNeasy Mini Kit and RNeasy Plus Micro Kit - Having low yields of RNA (reply: 8)
  565. How to prevent RNA degradation in whole blood - (reply: 3)
  566. RNA extraction - (reply: 1)
  567. Freeze thawing RNA - (reply: 4)
  568. RNA appearance on 1.5% gel after DNase I digestion - (reply: 2)
  569. Remove RNA after DNA mini-prep - (reply: 1)
  570. RNA from Sheep and Bovine blood - Isolation of RNA from bovine and ovine whole blood (reply: 3)
  571. Proteinase K to improve RNA extraction yield - (reply: 2)
  572. cloudy phase during RNA extraction with Trizol - (reply: 10)
  573. how to prepare RNase from powder form? - (reply: 9)
  574. RNA isolation following apoptotic cell labeling - (reply: 3)
  575. get rid of RNA from Maxi - (reply: 1)
  576. RNA labeling - (reply: 3)
  577. Best method to concentrate RNA/cDNA? - (reply: 5)
  578. RNA electrophoresis - (reply: 3)
  579. RNA Extraction & EMSA - (reply: 1)
  580. RNA isolation form HUVEC - UGLY RNA!! (reply: 4)
  581. RNA isolation from jellyfish tissue - Anyone has experience? (reply: 1)
  582. RNA Degradation - DNAse I Treatment, 260/230 ratios (reply: 4)
  583. Tissue sonication for RNA extraction - Help :) (reply: 1)
  584. RNA integrity - (reply: 7)
  585. DNA/RNA into nucleotides - (reply: 6)
  586. denature RNA and miRNA Q-PCR - (reply: 1)
  587. Old threads on "siRNA, microRNA and RNAi" are achived here - (reply: 3)
  588. RNA extraction in -80 - (reply: 1)
  589. RNAi in bacteria - Why no finding for gene silencing in bacteria? (reply: 1)
  590. RNase free syringe for Qiagen DNase treatment, could not find any company provid - (reply: 2)