Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
RNA Related Discussions
  1. RNA purification with TRI_ low yield - (reply: 1)
  2. bacterial RNA isolation from PBS buffer water - (reply: 1)
  3. 2 types of cell lines for RNA extraction - (reply: 1)
  4. RNA Gel 3 Sharp bands? - (reply: 1)
  5. Gel Electrophoresis and RNA - (reply: 1)
  6. BLOCK-iT Pol II miR RNAi system: Issues with generating stable knockdown - (reply: 1)
  7. Quantuitative staining of RNA and DNA? - (reply: 4)
  8. How to prepare a "home-made" lysis solution compatible with RNA extracti - (reply: 1)
  9. haeting RNA before electrophoresis - (reply: 1)
  10. Viral RNA in total RNA eluate for transfection - (reply: 1)
  11. Increasing RNA yield from very small tissues - (reply: 3)
  12. tripure rna isolation - (reply: 1)
  13. RNA agarose gel electrophoresis came up empty - (reply: 10)
  14. commercially available miRNA or small RNA? - (reply: 2)
  15. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
  16. Nanodrop question for RNA - (reply: 13)
  17. RNA Precipitation - (reply: 1)
  18. RNA lab - (reply: 1)
  19. What is the protocol to remove RNA contamination (Using RNase) from DNA? - (reply: 8)
  20. What is the RNA:Protein ratio for ONE gene over different RNA levels? - (reply: 1)
  21. Primer Design for RNA probes - (reply: 2)
  22. Real time PCR for degraded RNA - (reply: 1)
  23. RNA contamination in plasmid extracted - (reply: 1)
  24. Any idea of the composition of the RNA Wash Buffer from Zymo Research RNA Clean - (reply: 1)
  25. help with total RNA isolation - (reply: 1)
  26. Protein quantification after TRIzol Extraction - (reply: 2)
  27. Water RNA Contamination? - (reply: 4)
  28. Low RNA quantity after on column DNAse digestion - (reply: 1)
  29. Checking RNA integrity by Denaturing Gel - (reply: 2)
  30. Small yield of RNA after on column DNAs digestion , Why - (reply: 3)
  31. RNA Freeze/Thaw and NanoDrop Concentration - (reply: 1)
  32. software for making RNA secondary structure - (reply: 1)
  33. RNA integrity and RNA fragmentation? - (reply: 1)
  34. RNA isolation, dilution question - (reply: 1)
  35. small rna query - (reply: 1)
  36. rna thawing - (reply: 4)
  37. Cleaning of cuvettes (spectrophotometer) for RNA measurement - (reply: 7)
  38. RNA isolation from whole blood - (reply: 2)
  39. RNA concentration given by Nanodrop - (reply: 9)
  40. RNA yield and quality: Trizol vs. Column based - (reply: 2)
  41. Need help on RNA preparation for RNA sequencing - (reply: 7)
  42. what is the possible mechanism for a downregulation of RNA expression - (reply: 4)
  43. rna extraction protocol - (reply: 3)
  44. Preparation of RNase A From Powder - (reply: 1)
  45. miRNA-mediated competing endogenous RNAs (ceRNAs) networks - (reply: 4)
  46. RNA seq reads - assessing distance from start codon - (reply: 1)
  47. question about RNA concentration for real time PCR - (reply: 1)
  48. RNA extraction from flat worms - (reply: 2)
  49. Comprehensive List of long noncoding RNA(lncRNA) resources - (reply: 2)
  50. Is there any alternative to Formamide in RNA Gel Electrophoresis? - (reply: 1)
  51. RNAse - how to convert ug/ml to U/ml? - (reply: 3)
  52. rna isolation from formalin fixed peg tissue - (reply: 2)
  53. software for drawing small RNA molecules and nucleotides - (reply: 2)
  54. low RNA A260/280 - (reply: 2)
  55. RNA concentration quiries - (reply: 2)
  56. Removing ~200 different transcripts from total RNA pool - (reply: 1)
  57. RNase digestion followed by Radiolabelling iCLIP/CLIP - (reply: 1)
  58. Spleen homogonization for RNA extraction issue - (reply: 1)
  59. cDNA preparation from degraded RNA - (reply: 2)
  60. excess rna sample affect qpcr? - (reply: 2)
  61. How to ship plant tissue for RNA extraction? - (reply: 2)
  62. Concentration of cDNA from unknown quantity of RNA by mistake - (reply: 7)
  63. RNA extraction for RT-PCR - (reply: 3)
  64. Simultaneous RNA and DNA transfection? - (reply: 2)
  65. q value in rna seq - (reply: 1)
  66. Diffuse rRNA bands on RNA denaturing (formaldehyde) gel - (reply: 5)
  67. How to get rid of DNA contamination in RNA prep - (reply: 4)
  68. 100 ng RNA for cDNA synthesis - (reply: 4)
  69. RNA extraction from human jaw bone - (reply: 4)
  70. How to avoid RNA contamination in DNA samples:? - (reply: 1)
  71. RNA contamination in DNA samples - (reply: 2)
  72. Common causes for low RNA A260/230 ratios - (reply: 7)
  73. interpreting RNA agarose gel results? - (reply: 1)
  74. Basic question about qPCR and endogenous RNA control. - (reply: 4)
  75. Biotinylated RNA on Nylon Membrane - (reply: 2)
  76. Nonspecific RNA transcription assay - (reply: 3)
  77. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
  78. RNA concentration suddenly drops - (reply: 3)
  79. RNA concentration to start RT reaction - (reply: 1)
  80. Labeling and tracking RNA - (reply: 3)
  81. Absurdly high A260/280 ratio for RNA - (reply: 2)
  82. accidentally used denatured ethanol for DNA and RNA - what to do? - (reply: 4)
  83. Artificial RNA as normalizer (HK gene) - (reply: 1)
  84. Cannot isolate total RNA using column - (reply: 2)
  85. methods to extract RNA from blood sample? - (reply: 4)
  86. How to prepare serial dilutions of RNA or cDNA - (reply: 3)
  87. RNA extraction by trizol - (reply: 3)
  88. 16S ribosomal RNA sequences (Bacteria and Archaea) - (reply: 4)
  89. agarose gel electrophoresis of RNA - (reply: 1)
  90. Isolation of protein and RNA from RNeasy Minikits? - (reply: 5)
  91. RNA journals - (reply: 4)
  92. RNA storage time - (reply: 1)
  93. TRIZOL trouble - (reply: 2)
  94. RNA extraction - (reply: 3)
  95. micro RNA - (reply: 1)
  96. RNA alcohol/salt precipitation...low temperature - (reply: 1)
  97. long non-coding RNAs biogenesis pathways - (reply: 1)
  98. Extracting total RNA from woody plant roots - (reply: 2)
  99. Problem with precipitating RNA from ethanol solution - (reply: 1)
  100. Buffers RNase decontamination - (reply: 1)
  101. Homozygote DNA but Heterozygote RNA? - (reply: 2)
  102. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
  103. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
  104. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
  105. Long term storage for RNA extracted from pancreas - (reply: 3)
  106. Viral RNA extraction/isolation methods from plant tissue - (reply: 4)
  107. Why RNase H treatment after cDNA synthesis - (reply: 2)
  108. RNA isolation from Sesame seed - (reply: 3)
  109. Homogenize pancreas for RNA extraction - (reply: 3)
  110. Purification of RNA binding proteins by avidin agarose beads - (reply: 2)
  111. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
  112. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
  113. Amount of RNA (ng) for good ct values. - (reply: 3)
  114. polyacrylamide RNA gel - (reply: 4)
  115. Is my RNA degraded? - (reply: 7)
  116. Problems with extraction of RNA from primary culture - (reply: 4)
  117. Sanger Sequencing RNAse Interference? - (reply: 1)
  118. RNA isolation - (reply: 1)
  119. Low yield for small RNA isolation - (reply: 1)
  120. RNA extraction: low RCF by mistake - (reply: 1)
  121. Visualizing RNA on blot - (reply: 1)
  122. Question about RNA and expression - (reply: 5)
  123. RNase inactivation during IHC prior to LCM - (reply: 4)
  124. Protein and RNA interaction - (reply: 1)
  125. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
  126. Large scale Viral RNA purification Kit - (reply: 3)
  127. minimum amount of RNA for RT_PCR - (reply: 1)
  128. Reference RNA for qPCR - (reply: 3)
  129. RNA isolation of murine pancreatic islets - poor ratios - (reply: 3)
  130. Checking pH of RNase Free Solutions - (reply: 1)
  131. Atypical yeild of RNA - (reply: 1)
  132. Problems with RNA extraction from Sorghum anthers/pollen - (reply: 1)
  133. How does DEPC work to inactivate RNases - (reply: 3)
  134. Does Proteinase K digest RNAse in lysis buffer? - (reply: 2)
  135. RNA pellet gone in the wrong concentration of ethanol - (reply: 4)
  136. Problems in RNAi construction by using pUC19 - (reply: 3)
  137. [QUESTION] Is it possible to incorperate Tri Reagent (Trizol) with the Mirvana m - (reply: 3)
  138. Detection of T7 RNA polymerase by WB - (reply: 6)
  139. RNA isolation from small numbers of cells - (reply: 6)
  140. Problems with Electrophoresis of RNA - (reply: 3)
  141. Extraction of RNA from HEPG2 - (reply: 1)
  142. Synthesis of carrier RNA(poly-A) for DNA Extraction - (reply: 2)
  143. 5'RACE of RNA Pol3 transcripts? - (reply: 3)
  144. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  145. RNA integrity - (reply: 5)
  146. Question about PCR - DNA or RNA as template - (reply: 4)
  147. Dyes for RNA in Urea-polyacrylamide gel - (reply: 1)
  148. Incorporating RNAse A into DNA extraction - (reply: 2)
  149. Agarose gel electrophoresis of RNA - (reply: 5)
  150. Recovery of viral RNA - (reply: 3)
  151. Ethanol contamination in RNA - (reply: 11)
  152. blood RNA - (reply: 2)
  153. RNA extraction kits for small samples - (reply: 6)
  154. RNA caculation!! - (reply: 2)
  155. RNA in situ hybridization: pH of AP staining buffer? - (reply: 1)
  156. Issues with Cy5 labeled RNA - (reply: 2)
  157. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  158. How to precipitate RNA from water - (reply: 3)
  159. FFPE tissue and RNA isolation - (reply: 2)
  160. Calculation of RNA - (reply: 2)
  161. My RNA extraction failed (From a newbie ) - (reply: 2)
  162. RNase h electrophoresis - (reply: 1)
  163. Trizol and safety - (reply: 7)
  164. miRNA from plasma_purity problem after RNA isolation - (reply: 1)
  165. RNA degradation problem, only one band on the gel - (reply: 3)
  166. Application of RNase - (reply: 3)
  167. RNAi in combination with adenoviral-mediated over-expression - (reply: 1)
  168. RNA transcription from T3 promoter problem - (reply: 1)
  169. How to purify RNA from a handful number of cells? - (reply: 5)
  170. RNA purification from urine samples - (reply: 2)
  171. Ideas for determining poly(a) status of RNA - (reply: 2)
  172. Is some amount of RNA obligatory for qRT-PCR? - (reply: 5)
  173. Purifying RNA from Solution with RNEasy? - (reply: 2)
  174. Is it possible to extract RNA from Cells frozen in 10% DMSO ? - (reply: 2)
  175. how to design primers for 16sr RNA - (reply: 24)
  176. Isolation of both DNA and RNA from the same plant tissue - (reply: 8)
  177. RNase Band in old plasmid stored at -40 - (reply: 2)
  178. How to lysis cells for an RNA extraction - (reply: 1)
  179. protein solubilization and quantitation after extraction using Trizol - (reply: 2)
  180. Urgent - RNA extraction / RNase contamination ? - (reply: 4)
  181. RNA transfer to nitrocellulose membrane (Northern blot) - (reply: 2)
  182. precipitation of RNA after DNase treatment - (reply: 3)
  183. Can we use denatured DNA standards on RNA formamide gels - (reply: 2)
  184. RNA immunoprecipitation DNase treatment - (reply: 4)
  185. RNA extraction protocol help - (reply: 1)
  186. Higher mRNA level after RNAi? - (reply: 1)
  187. question: RNAi for bacteria? - (reply: 3)
  188. Control for RNA chip experiment - (reply: 1)
  189. High RNase concentration interfering with PCR? - (reply: 8)
  190. RNAi problem - Gene is knocked down, but not protein - (reply: 8)
  191. Mortar and Pestle for RNA Extraction - (reply: 1)
  192. Optimizing Trizol mRNA extraction - (reply: 3)
  193. sample preservation for RNA extraction - (reply: 1)
  194. RNA extraction from blood stain - (reply: 1)
  195. RNA purity - (reply: 3)
  196. RNA Buffer Extraction - (reply: 3)
  197. Viral RNA Extraction from Tissue Culture Supernatant - (reply: 5)
  198. DNA extraction from cerebrospinal fluid using trizol - (reply: 2)
  199. New RNAi video from Nature - (reply: 1)
  200. RNA electrophoresis - (reply: 4)
  201. DNA and RNA extraction - (reply: 6)
  202. RNA extraction of ethanol preserved matherial - (reply: 1)
  203. RNA extraction from paraffin embedded tissue - (reply: 2)
  204. Designing of RNA probe for in situ hybridization - (reply: 1)
  205. RNA extraction from a 1mm brain tissue punch - uncertain about homogenisation - (reply: 2)
  206. total RNA Extraction gel control - (reply: 1)
  207. Isopropanol, Water, and RNA Stability - (reply: 3)
  208. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  209. Concentration of RNA to same amount across different samples - (reply: 5)
  210. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
  211. Dilution of 18s. How much? - (reply: 1)
  212. Difference between <Antisense RNA> & <RNAi>? - (reply: 1)
  213. Extrct RNA from fungus in broth medium - (reply: 2)
  214. Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
  215. RNAse treatment of genomic DNA - concentration and duration? - (reply: 1)
  216. protein did not knock down by shRNA, but RNA did knock down - (reply: 4)
  217. RNA extraction and purity from Drosophila - (reply: 1)
  218. Phenol peak at RNA isolation with Trizol method - (reply: 1)
  219. RNA extraction from human sperm - (reply: 3)
  220. Trizol and Protein extraction - (reply: 2)
  221. RNA extraction and preparation - RTqPCR - (reply: 3)
  222. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
  223. Strange question on TRIzol and RNAlater - (reply: 3)
  224. PAXgene blood RNA tube for RNA extraction - (reply: 1)
  225. RNA labeling with Biotin - (reply: 1)
  226. RT-PCR doesn't work with all RNA used - (reply: 1)
  227. RNA isolation from frozen cells - (reply: 2)
  228. RNA protein complex interaction in 4-12% NU PAGE gradient gel - RNA PROTEIN bEHAVIOR IN GRADIENT GELS (reply: 1)
  229. How many RNA I need for detecting plasma miR expression? - (reply: 1)
  230. To isolate DNA, RNA and Protein from same culture flask for methylation study? - (reply: 2)
  231. No gene amplification, 18s amplification is fine - (reply: 1)
  232. Totai RNA dilution - (reply: 8)
  233. RNA from guinea pig lung=degraded - (reply: 5)
  234. Tissue disruption - for RNA isolation (reply: 2)
  235. cells stably expressing T7 RNA polymerase - (reply: 1)
  236. RNA degradation during electrophoresis?? - (reply: 2)
  237. Total RNA estimation - (reply: 2)
  238. Bioanalyzer and RNA gel - (reply: 2)
  239. Genomic DNA contamination in my RNA sample? - (reply: 8)
  240. could i use sterile deionized water instead of DEPEC water in RNA extraction - (reply: 6)
  241. RNase should be added in which step for gDNA isolation using phenol chloroform m - after lysozyme / preteinase k treatment or after phenol chloroform? (reply: 9)
  242. Checking RNA on gel after DIG labeling - (reply: 1)
  243. Whole blood RNA and DNA extraction help - Expression analysis and epigenetics (reply: 2)
  244. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
  245. is it degraded RNA? - I dont get a clear 3-band after electrophoresis (reply: 6)
  246. TRIZol Reagent additional reagents & protocol clarifications - (reply: 4)
  247. Loss of RNA conc. and quality in Qiagen RNeasy Protocol - (reply: 4)
  248. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
  249. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
  250. template transfer? - how does the rna polymerase switches template? (reply: 1)
  251. RNA precipitation - (reply: 1)
  252. RNA extraction from PBMC layer - how to increase RNA Concentration during RNA extraction from BLOOd (reply: 1)
  253. Nuclear or Cytoplasmic RNA Purification - (reply: 1)
  254. Is DNAse I step necessary after RNA synthesis by in vitro transcription? - (reply: 1)
  255. Remove gDNA from RNA sample - (reply: 3)
  256. Best Total RNA Isolation Kit - For plants, and tiny samples <10mg (reply: 3)
  257. Accidentally add isopropanol into Trizol - (reply: 1)
  258. 18s as endogenous control for Oligo dT RT - Can we use 18s ? (reply: 2)
  259. Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els (reply: 4)
  260. oocye extraction and RNA isolation - (reply: 1)
  261. siRNA screening and RNAse free - (reply: 1)
  262. the red dye in trizol? - (reply: 2)
  263. Sequencing of 18srRNA - (reply: 2)
  264. Can very low RNA concentration cause Ct bias..? - (reply: 3)
  265. RNA carrier when using not so good DNA for bisulfite conversion - (reply: 3)
  266. Unstable A260/A230 in RNA isolation - (reply: 2)
  267. Few basic questions about RNA - (reply: 3)
  268. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
  269. RNA Isolation - (reply: 3)
  270. Manipulating RNA Concentrations/Amounts - (reply: 6)
  271. Why does T7 RNA transcription start with a GGG? - (reply: 5)
  272. Internal Control RNA or DNA - (reply: 4)
  273. RNA extraction - (reply: 3)
  274. can RNA amplify in two-step qPCR? - (reply: 2)
  275. Total RNA only produce 1 band in gel - (reply: 2)
  276. RNAi showing upregulation via QPCR - Help needed (reply: 3)
  277. What causes RNA/DNA to unbind from silica membrane? - (reply: 1)
  278. RNA extraction troubleshooting: low 260/230 - (reply: 1)
  279. RNA in native gel - (reply: 3)
  280. RNA extraction from FTA classic - (reply: 1)
  281. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
  282. TRIzol RNA extraction- What is in the top layer? - (reply: 1)
  283. RNA extraction Question - (reply: 2)
  284. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
  285. Possible solvents for RNA - (reply: 2)
  286. Pooled RNA from healthy controls - delta-delta Ct method (reply: 1)
  287. Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
  288. RNAi is somatic and germline cells - (reply: 3)
  289. How to isolate serum from blood for RNA extraction - (reply: 2)
  290. RNA/cell accumulation of difficult-to-obtain tissue - (reply: 1)
  291. generating cDNA from human brain RNA - (reply: 1)
  292. rna isolation - (reply: 2)
  293. Amplification of region from sperm RNA - sperm RNA stability (reply: 4)
  294. Amplification of 3.4 kb product from RNA for cloning - (reply: 5)
  295. RNA isolation of spleen and thymus: RNA degradation - (reply: 2)
  296. Prepare p:C:I - for RNA extraction (reply: 5)
  297. DNA contamination of RNA samples for qPCR...weird results! - (reply: 3)
  298. Washing RNA pellet - 85% ethanol? - (reply: 2)
  299. Viral RNA is relatively stable than mRNA from cells? - (reply: 1)
  300. Isolating bacterial RNA and protein from the host tissue sample - (reply: 3)
  301. Smears above ribosomal RNA bands - RNA extraction from microalgae (reply: 2)
  302. Trizol Extraction of PBMCs, low 260/230 - (reply: 5)
  303. TRIzol phase extraction with Lock-Phase tubes - Does anyone use Lock-Phase? (reply: 1)
  304. Tissue homogenization buffer to keep viral RNA intact - (reply: 6)
  305. RNA degradation in collagen gels - (reply: 1)
  306. RNA pellet white and low ratio (low purity) - how to improve RNA purity (reply: 5)
  307. nuclear and cytoplasmic RNA - (reply: 2)
  308. rna isolation - (reply: 1)
  309. how does rna precipitate in LiCl solution ? - (reply: 1)
  310. RNA Pol II CHIP protocol help - suggest protocol (reply: 2)
  311. Making solutions for RNA work - (reply: 1)
  312. RNA quality - (reply: 3)
  313. RNA quality - (reply: 3)
  314. How to check RNA quality - (reply: 5)
  315. Cleaning RNase - How do i clean out the RNase from my genomic DNA (reply: 1)
  316. Looking for recommendation for total RNA extraction kit - (reply: 4)
  317. RNA: Is this degratation or something else? - (reply: 1)
  318. rna isolation - (reply: 1)
  319. mRNA preparation from total RNA - a role of poly A in mRNA preparation from total RNA (reply: 2)
  320. Trizol stability - (reply: 1)
  321. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)
  322. RNA fragmentation - (reply: 3)
  323. RNA extraction - how many cells do I need at least?? (reply: 4)
  324. RNA extraction protocol for small amounts of cells - (reply: 2)
  325. cDNA prepared with varying amounts of RNA - is the amount of cDNA produced proportional to the input RNA? (reply: 3)
  326. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
  327. COMPLETE RNA degradation - (reply: 18)
  328. Protein shows KD but RNA level is higher - (reply: 4)
  329. RNA extraction from stained cells - (reply: 2)
  330. low yeild fo RNA after invitro transcription - (reply: 4)
  331. RNA extraction: no pellet... - (reply: 2)
  332. Rnase A for plasmid miniprep - cheaper alternative to qiagen? (reply: 4)
  333. problem with Trizol RNA extraction - (reply: 7)
  334. rnase treatment - (reply: 2)
  335. Trizol isolation of RNA from OCT-embedded frozen tissue - Question (reply: 1)
  336. RNA extraction from trout embryos - (reply: 3)
  337. Which way to clean up TRIzol-extracted RNA? - Ethanol precipitate it first or not? (reply: 2)
  338. RNA extraction expertise needed! - (reply: 2)
  339. Frozen RNA precipitated with ethanol and NaAc! - RNA precipitation (reply: 2)
  340. rna isolation manually - (reply: 2)
  341. Is it sufficient to heat inactivate DNases and RNases? - (reply: 4)
  342. Primers for yeast (18S) - (reply: 1)
  343. problem with Fast Prep Isolation of RNA - problem with Fast Prep Isolation of RNA (reply: 2)
  344. Insect larvae storage for RNA Isolation - looking for a cheap.. easy way.. something innovative!!!!!&# (reply: 4)
  345. RNA solutions - Calculating concentrations (reply: 9)
  346. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
  347. Sample handling for RNA extraction - (reply: 1)
  348. Normalising RNA concentrations - Help (reply: 4)
  349. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
  350. total RNA yield - (reply: 3)
  351. DNase 1 treratment of RNA - (reply: 11)
  352. Concentrating RNA - (reply: 3)
  353. RNA:sharp bends and a smear - (reply: 1)
  354. Measuring DNA, RNA, protein and cell wall synthesis - (reply: 1)
  355. RNA extraction - (reply: 2)
  356. Trizol RNA extraction - (reply: 2)
  357. DNA contamination in RNA samples - (reply: 2)
  358. Extraction of RNA from blood samples - Problems (Trizol method) (reply: 1)
  359. Measurement of RNA concentration after Dnases treatment - (reply: 4)
  360. RNA Probe precipitation - (reply: 9)
  361. RNA immunoprecipitation...HELP! - (reply: 4)
  362. Normalizing RNA concentrations prior to cDNA production - qPCR and making cDNA (reply: 1)
  363. Can't get clean RNA prep with TRIzol - (reply: 2)
  364. RNA from Zebrafish - (reply: 3)
  365. DNA sonication - RNA contamination? - New probe, start all over (reply: 13)
  366. Rnase for flow cytometry - (reply: 1)
  367. Detecting small RNAs via DeepSeq? - (reply: 1)
  368. extraction of viral rna from plant tissue - (reply: 1)
  369. Drying RNA in Speedvac - (reply: 1)
  370. TRIzol v. TRI reagent - (reply: 2)
  371. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
  372. 10X MOPS buffer for RNA gels - It is yellow! (reply: 3)
  373. RNA isolation with peqGold TriFast - (reply: 2)
  374. storing RNA at -20C? - (reply: 4)
  375. RNA Extraction - Extraction of Yeast RNA from mice blood (reply: 1)
  376. How to remove urea from PAA gel? - RNA silver staining (reply: 1)
  377. Large Difference between Ct of 18s rRNA and Target Gene - (reply: 2)
  378. RNA gel to test DIG labeled RNA probe - (reply: 1)
  379. wacky RNA gel - (reply: 1)
  380. RNA marker for isotope labeled - (reply: 2)
  381. Human 18s rRNA - Human 18s rRNA and qRTPCR normalization (reply: 7)
  382. How to remove diphosphate from in vitro transcribed RNA? - (reply: 1)
  383. Reverse-transcription of UV-damaged RNA - (reply: 3)
  384. evolutionarily conserved RNA secondary structures in multiple sequence alignment - how to (reply: 2)
  385. cDNA concentration higher than original RNA - (reply: 4)
  386. 18s control in qPCR - (reply: 2)
  387. DENATURING AGAROSE GEL FOR RNA - (reply: 1)
  388. circular RNA - (reply: 3)
  389. total RNA (for miRNA) extraction from human serum - (reply: 11)
  390. How do i increase my rna yield - How do i increase my rna yield (reply: 3)
  391. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
  392. how to get higher RNA purity? - (reply: 3)
  393. Snap freezing worm samples for RNA prep - is it essential? (reply: 1)
  394. Does RT step smplify RNA - (reply: 2)
  395. gene expression, reverse transcription on total RNA or mRNA? - (reply: 3)
  396. Low RNA yield after extraction by RNA blood mini kit. - (reply: 2)
  397. RNA recovery from Ethanol - (reply: 2)
  398. Question about the control for an RNAi Experiment - (reply: 3)
  399. Plant RNA Isolation Aid - (reply: 3)
  400. freezing during RNA isolation step - (reply: 1)
  401. Low 260/230 ratio in my RNA - (reply: 2)
  402. Low 260/230 ratio in my RNA - (reply: 3)
  403. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
  404. No RNA after Trizol extraction - Lamba max: 230 !!! (reply: 8)
  405. Doubt: Trizol protocol - about certain steps (reply: 9)
  406. Bacteria Lysis Reagent For RNA extraction - Need Expert's Opinion (reply: 12)
  407. PLASMA RNA extraction - (reply: 1)
  408. DNA contamination after RNA extraction, which kit works best? - (reply: 6)
  409. Handling RNA - How to avoid RNase contamination - (reply: 17)
  410. Phenol chloroform ratio during RNA isolation - (reply: 1)
  411. Removal of RNA from DNA sample - (reply: 15)
  412. RNA gel electrohoresis - (reply: 1)
  413. Verified RNAi Sequences - (reply: 4)
  414. DNA and RNA contamination in RT PCR water controls - (reply: 2)
  415. rna from plasmid - (reply: 1)
  416. Best time to isolate RNA for qPCR? - (reply: 1)
  417. Trizol/triReagent Question - (reply: 2)
  418. Variation between spectros - tot RNA concentrations (reply: 1)
  419. Glycogen and speedvac drying - to make sure no RNA loss during speedvac drying (reply: 6)
  420. RNA from MMA embedded calcified bone - (reply: 1)
  421. low od260/230 for rna - (reply: 1)
  422. RNA - bad 230/280 and 260/280 ratio - what to do?? (reply: 3)
  423. RNA gel- nothing to see - (reply: 2)
  424. heating rna before rt reaction? - (reply: 1)
  425. RNA storage - (reply: 3)
  426. RNase treatment before analyzing sonicated chromatin in ChIP - (reply: 2)
  427. automating RNA isolation - Has anyone done automation of yeast RNA isolation at any stage??? (reply: 2)
  428. miRNA and TRIZOL - (reply: 7)
  429. RNA dot blot - (reply: 6)
  430. RNA not visible on gel :( - (reply: 3)
  431. 260/230 ratio - Very low amount of RNA - how to improve 260/230 ratios? (reply: 5)
  432. RNA impossible to dissolve - (reply: 2)
  433. rna storage at -20 - (reply: 2)
  434. Evaluate My Protocol: RNA Extraction w/ Fat. - (reply: 1)
  435. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
  436. si-Fi, Software for RNAi off-target prediction - (reply: 2)
  437. RNA Extraction Mice Adipose Tissue - (reply: 3)
  438. Use of 70% ethanol to remove RNAse from working area - (reply: 2)
  439. Cells to Ct RNA isolation kit (Ambion) - (reply: 1)
  440. RNA elution after stains all staining - (reply: 3)
  441. rna degradation - what goes wrong - (reply: 2)
  442. rna clean up - what is the rna recovery - (reply: 2)
  443. Fast protocol for RNA agarose gel electrophoresis - (reply: 1)
  444. mirVana kit - I can't have small RNA isolated!!! (reply: 5)
  445. RNA extraction 260/230 ratio - (reply: 6)
  446. Experimental Design Problem for RNA FISH - (reply: 1)
  447. concentrating RNA samples - (reply: 7)
  448. RNA, mRNA, sequencing... HELP - (reply: 2)
  449. how to prepare RNAse A from powdered form - (reply: 1)
  450. RNA Purification - After DNase (reply: 3)
  451. Homogenation of Mouse Spinal Cord Tissue for RNA Extraction - Most effective way to do it? (reply: 3)
  452. small RNA Northern - duration of transfer (reply: 1)
  453. rna isolation - (reply: 1)
  454. RNA Isolation - RNA isolation from human cell lines (reply: 2)
  455. Pooling doesn't work - make up rna amount of 10ug (reply: 3)
  456. RNase: RNA ratio - making dsRNA (reply: 1)
  457. How many cells... - ...gives you how much RNA? (reply: 2)
  458. qPCR and quantity of RNA - (reply: 2)
  459. extracting protein from trizol - (reply: 2)
  460. RNA isolation from monocytes - (reply: 1)
  461. Pooling RNA/precipitation/RNA cleanup - advise needed (reply: 1)
  462. RNAi - (reply: 1)
  463. RNA extration from plant - Lower yield... (reply: 1)
  464. How much RNA is needed to see rRNA on a gel? - (reply: 3)
  465. store whole blood with tri-reagent too long caused RNA degradation or poor RNA q - (reply: 1)
  466. T4 RNA ligase - Question about substrate (reply: 13)
  467. how to pool RNA? - (reply: 1)
  468. Is it Possible - Is it possible RNAi cross-reaction with normal cells molecules?? (reply: 1)
  469. pancreas RNA gel run consult - (reply: 1)
  470. Rnase for cell cycle - (reply: 5)
  471. Help: Advices on extracting RNA from pellets? - (reply: 3)
  472. Trizol issues! - Samples are not clean enough after isolation!!! (reply: 2)
  473. RNA quantification in microplate reader - (reply: 3)
  474. tri-reagent rna isolation - (reply: 1)
  475. Trizol RNA/DNA extraction - (reply: 3)
  476. Drosophila Northern Blot and RNA questions - (reply: 2)
  477. RNA isolation for real-time RT-PCR - forgot to inactivate the DNAse (reply: 4)
  478. Harvest intracellular bacterial RNA - (reply: 2)
  479. Plant RNA extraction problem - A cry for help. (reply: 2)
  480. Designing new RNAi - (reply: 2)
  481. RNA reverse transcription riddle - (reply: 6)
  482. Phenol - why different for DNA and RNA extraction? (reply: 9)
  483. PCR, RNA, Northern Blotting???? - (reply: 1)
  484. 2 step RNA isolation - Possible to Isolate RNA in 2 days? (reply: 2)
  485. Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
  486. RNA visualization - (reply: 1)
  487. Genomic DNA contamination of RNA - (reply: 24)
  488. 18S changes with cDNA dilution - (reply: 2)
  489. RNA precipitation problem - (reply: 2)
  490. Challenging question for RNA people - (reply: 1)
  491. RNA contamination in PCR - (reply: 1)
  492. High salt solution to precipitate RNA - (reply: 1)
  493. RNA extraction using TRIzol - (reply: 19)
  494. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
  495. Reverse transcribing organically contaminated RNA - (reply: 2)
  496. Do you lose RNA by quick freezing? - (reply: 5)
  497. No RNA from cell pellet using Qiagen MiniKit!!??!! - (reply: 5)
  498. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  499. RNA extraction for microarray - (reply: 25)
  500. about the total RNA isolation (trizol methods) and further Dnase I - (reply: 3)
  501. RNA cleanup methods (WITHOUT USING KITS!!) - protocol for removing phenol contamination (reply: 5)
  502. Ask for help about the RNA purification protocol by phenol/chlorform methods - Ask for help about the RNA purification protocol by phenol/chlorform m (reply: 2)
  503. RNA extraction - Which is which? (reply: 6)
  504. So confused with RNAi - (reply: 3)
  505. Pancreas RNA extraction - improve protocol needed (reply: 2)
  506. Genomic DNA contamination in RNA sample - (reply: 2)
  507. no 18S band on RNA agarose gel - (reply: 1)
  508. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
  509. multiple bands in my RNA sample? - (reply: 2)
  510. multiple bands in my RNA sample? - (reply: 2)
  511. Principles behind DNA RNA protein isolation and purification - (reply: 2)
  512. RNA isolation form plants - (reply: 4)
  513. 5' end RNA biotin labeling - need protocol (reply: 2)
  514. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
  515. Heating RNA samples for electrophoresis - (reply: 3)
  516. Removing phenol contamination from total RNA - (reply: 9)
  517. how to prepare rnase? - (reply: 6)
  518. Viscous RNA samples - (reply: 1)
  519. Any mRNA amplification kit for microarray? - I have very little amount of RNA but have to submit it for microarray. (reply: 4)
  520. RNAi in Zebrafish - vector for zebrafish knock-down (reply: 4)
  521. RNA isolation -what is happening?- - (reply: 5)
  522. RNA isolation from murine pancreas - (reply: 2)
  523. Qiagen's RNeasy Mini Kit and RNeasy Plus Micro Kit - Having low yields of RNA (reply: 8)
  524. How to prevent RNA degradation in whole blood - (reply: 3)
  525. RNA extraction - (reply: 1)
  526. Freeze thawing RNA - (reply: 4)
  527. RNA appearance on 1.5% gel after DNase I digestion - (reply: 2)
  528. Remove RNA after DNA mini-prep - (reply: 1)
  529. RNA from Sheep and Bovine blood - Isolation of RNA from bovine and ovine whole blood (reply: 3)
  530. Proteinase K to improve RNA extraction yield - (reply: 2)
  531. cloudy phase during RNA extraction with Trizol - (reply: 10)
  532. how to prepare RNase from powder form? - (reply: 9)
  533. RNA isolation following apoptotic cell labeling - (reply: 3)
  534. get rid of RNA from Maxi - (reply: 1)
  535. RNA labeling - (reply: 3)
  536. Best method to concentrate RNA/cDNA? - (reply: 5)
  537. RNA electrophoresis - (reply: 3)
  538. RNA Extraction & EMSA - (reply: 1)
  539. RNA isolation form HUVEC - UGLY RNA!! (reply: 4)
  540. RNA isolation from jellyfish tissue - Anyone has experience? (reply: 1)
  541. RNA Degradation - DNAse I Treatment, 260/230 ratios (reply: 4)
  542. Tissue sonication for RNA extraction - Help :) (reply: 1)
  543. RNA integrity - (reply: 7)
  544. DNA/RNA into nucleotides - (reply: 6)
  545. denature RNA and miRNA Q-PCR - (reply: 1)
  546. Old threads on "siRNA, microRNA and RNAi" are achived here - (reply: 3)
  547. RNA extraction in -80 - (reply: 1)
  548. RNAi in bacteria - Why no finding for gene silencing in bacteria? (reply: 1)
  549. RNase free syringe for Qiagen DNase treatment, could not find any company provid - (reply: 2)