Labeling and tracking RNA - (Jun/04/2013 )
I am trying to find a way to fluorescent-label RNA molecules and track them in the cell by microscopy. My idea is to synthesize the RNA (in-vitro transcription) and transfect them into the cell. I know that you can incorporate fluorescent labeled dNTPs into the RNA (like the way one would synthesize RNA-FISH probes), but is the RNA structure/function would be unaltered? Is there any method for adding a fluorescent label to the end of the RNA, without messing with the main portion of it?
Thank you for your time
check this webpage, it may contain the information you want:
life technologies, molecular probes, rna labeling
This seems appropriate:
These kits are probably more economical: http://www.markergene.com/oliglo2.php