RNA extraction protocol help - (Feb/14/2012 )
Just a quick question. For the protocol for my RNA extraction I have to use 96-100% ethanol for a certain step. For my first set of extractions, someone just gave me a tube of ethanol to use but I recently ran out. We have 100% in the lab, however, it comes in metal cans. I don't know much about RNases, but wouldn't there be RNases in the ethanol? Or are RNases destroyed by high concentrations of ethanol? In that case how do you get "RNase" free ethanol?
The New-brought ethanol is regarded as the RNase free.