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Cleaning of cuvettes (spectrophotometer) for RNA measurement - (Jan/23/2014 )

Hi People.

 

I have always just been measuring concentrations on a nanodrop so never really thought about doing it on the spec. However at my current location I have to measure this in a spec. So my question is for you who have experience in this, how much cleaning between samples are needed? There is only two cuvettes accesable for RNA/DNA measurements. And what solution do you use for cleaning? EtOH? NaOH,Tris-, EDTA combi?

-Kongen-

Are you attempting to recover samples after the reading or not?

-phage434-

are you using plastic or glass or quartz cuvettes?

 

do you have a cuvette cleaner (connected to vacuum, with a side-arm flask)?

 

how (and how often) do you clean the nanodrop head?

 

(btw- just because i'm a nitpicker- the nanodrop is a spectrophotometer, just doesn't use cuvettes).

-mdfenko-

No, im not trying to recover the samples. I use quartz, dont have a cuvette cleaner. And true that, and must admit I have never cleaned it, I wasnt responsible for maintaining it, so i have basically just been wiping it between uses with water.

-Kongen-

you may want to purchase a cuvette cleaner (here's one place).

 

we flush with water then dry by flushing with ethanol (95%) or acetone and allowing to dry on the cleaner.

-mdfenko-

I just rinse a few times with sterile water (fill, give a good flick of the wrist to empty).  I was taught never use EtOH on the quartz since it can precipitate DNA onto the glass.  

-rkay447-

after flushing with water there should be no dna to precipitate. the ethanol (or acetone) are to speed drying.

-mdfenko-

all above are obsession.

once rinsing with water is enough. 

-memari-