help with total RNA isolation - (Mar/12/2014 )
I am doing total RNA isolation from Corynebacterium Glutamicum, my problem is, after running TAE RNA agrose gel, I found 3 sharp bands on the gel.
I am attaching a pic of the gel... Sorry it was my first time so I loaded a bit too much sample in the gel...
I am using RiboPure™-Bacteria Kit and the samples r DNAse treated. please suggest..
So, what is the problem? These look (mostly) like typical RNA samples, with a 16S, 23S, and 5S/tRNA band.