RNase treatment before analyzing sonicated chromatin in ChIP - (Oct/21/2009 )
Dear All,
I just entered in the world of ChIP, and I am currently the only one in my lab to dare starting with this technique.
My question is:
After sonication, I wanted to see the DNA fragments range on agarose gel. So I run the RNase-untreated and RNase-treated DNA sample on 1% agarose gel.
I got one thick band in the lane where I did not add the RNase (no visible smear) while a small smear in the lane where I added RNase (thick band disappeared).
Is this thick band correspond to RNA presence?
I am unable to interpret this for the moment.
If any of you could help me, please
thanx
Juni
-Juni-
Juni on Oct 21 2009, 05:31 PM said:
Dear All,
I just entered in the world of ChIP, and I am currently the only one in my lab to dare starting with this technique.
My question is:
After sonication, I wanted to see the DNA fragments range on agarose gel. So I run the RNase-untreated and RNase-treated DNA sample on 1% agarose gel.
I got one thick band in the lane where I did not add the RNase (no visible smear) while a small smear in the lane where I added RNase (thick band disappeared).
Is this thick band correspond to RNA presence?
I am unable to interpret this for the moment.
If any of you could help me, please
thanx
Juni
I just entered in the world of ChIP, and I am currently the only one in my lab to dare starting with this technique.
My question is:
After sonication, I wanted to see the DNA fragments range on agarose gel. So I run the RNase-untreated and RNase-treated DNA sample on 1% agarose gel.
I got one thick band in the lane where I did not add the RNase (no visible smear) while a small smear in the lane where I added RNase (thick band disappeared).
Is this thick band correspond to RNA presence?
I am unable to interpret this for the moment.
If any of you could help me, please
thanx
Juni
Most likely, yes
Your DNA should look like a smear after you treat with RNase Clare
-Clare-
thanks Clare for your reply,
juni
-Juni-