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isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (Dec/30/2009 )

Hi all..

I am about to start RNA isolation from insect cells. I could not find a satisfactory protocol for this purpose. Can someone suggest a protocol to start with? I will also be doing some DNA isolations. My insect is a Lepidopteran and I plan to do the nucleic acid isolation from the larval stages.

thanks in advance.

-sowmi-

sowmi on Dec 31 2009, 07:24 AM said:

Hi all..

I am about to start RNA isolation from insect cells. I could not find a satisfactory protocol for this purpose. Can someone suggest a protocol to start with? I will also be doing some DNA isolations. My insect is a Lepidopteran and I plan to do the nucleic acid isolation from the larval stages.

thanks in advance.

A large number of methods have been described including those using commercially available kits (Trizol and RNAeasy for instance). Google "lepidopteran rna extraction".

Hope this helps
Attached File

-klinmed-

Thanks klinmed
I found few papers.. and I am working on the protocol..

-sowmi-