Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Biology

RNA yield and quality: Trizol vs. Column based - (Dec/25/2013 )

Hi Friends,


I would like to isolate RNA with both good yield and good quality. Tried Trizol and Qiagen column, so far I felt that Trizol had high yield but with limit on RIN value quality (like RIN>9). I wonder whether taking the advantage of Trizol's high yield and do further purification by column method.


I appreciate any comments about this hybrid approach on RNA's yield and quality,






You could always do a lithium chloride precipitation to clean up the RNA after TRIZOL. I would worry about a substantial loss in yield if you combine these protocols.


Thank you for your reply, Hoyajm

I will look into this method,