Protocol Online logo
Top : New Forum Archives (2009-): : siRNA, microRNA and RNAi

Detecting small RNAs via DeepSeq? - (Mar/12/2010 )


I was wondering whether there is a straightforward way to prepare total RNA libraries for DeepSeq that contain both mature miRNAs and pri- and pre-miRNAs? I know there are protocols to isolate small RNAs from RNA libraries via cutting bands of limited size from denaturing gels, 3' and 5' ligating them, etc., but those protocols tend to isolate a very limited size set of RNAs (e.g. 19-22 bp), and we are interested in isolating pri-, pre-, and mature miRNAs in one go. Is it possible? Thanks!


It is up to you to decide what size rang of RNA on the gel to enrich. If you want to include larger small RNAs, just include that size when cutting gels.