Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Biology

Accidentally add isopropanol into Trizol - (May/07/2011 )

RNA extraction. Accidentally adding isopropanol into trizol(with tissues) instead of chloroform.

Any chance I can rescue my samples?

Millions of thanks


Hmmm, not sure on this... I think the RNA will still be there, though it should have precipitated out and will be heavily contaminated with phenol and proteins. You could try just adding chloroform to get rid of the trizol and go from there, like you would if you were cleaning up a RNA sample for phenol contamination.