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RNA extraction of ethanol preserved matherial - (Dec/01/2011 )

Hi, I´m trying to extract RNA from matherial preserved in EtOH 100%. I´m using TRIZOL but it seems that the matherial is quite dificult to homogenize and disrupt since it is hard. Any suggestions?



You can always try to crush it deep-frozen in liquid nitrogen I guess, that should make it fragile. If it's not a bone.