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small RNA Northern - duration of transfer (Aug/30/2009 )


I've just started with the transfer of small RNA to nylon membrane. I do transfer at 10V using a semidry method for 1 hr, once the time exceeds for 15 mins and I saw the dye crossed the membrane to filter paper beneath , I wonder if the RNA can cross the membrane as well


I wouldn't think that to be the case... How small is the RNA?

Or you could always just conclusively determine this yourself - rinse the filter paper and do RT-PCR.


-Carlton H-