RNA extraction and purity from Drosophila - (Sep/23/2011 )
I am trying to extract RNA from the drosophila larvae and pupae for microarray. I want to run it on a gel to check its purity. But when i do that there is just a single band visible on the gel. Does any one have experience with Drosophila RNA work??
one of the responses in this seqanswers thread says:
"In case of drosophila, 2 close peaks at the 18 S and one smaller 28 S peak..I hope I'm right about larger the S unit size"
maybe your loading wasn't sufficient to see the 28S