Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Biology

rna isolation from formalin fixed peg tissue - (Nov/14/2013 )

a friend of mine posted this question at another forum and hasn't received any responses.

 

i would appreciate it if any of you can answer his question:

 

"Is anyone aware of any protocol for isolating RNA from formalin fixed polyethylene glycol embedded tissues?"

 

thanks

-mdfenko-

No specifics, but I think the PEG will probably preserve the RNA well.  I would attempt to just use a protocol for formalin fixed tissue, and do a bunch of washes beforehand to remove the PEG.

-bob1-

thanks, bob1. i'll make sure my friend sees your reply.

-mdfenko-