how to get higher RNA purity? - (Dec/30/2009 )
I extract RNA from plant leaves tissue using trizol protocol, my RNA purity OD260/280 around 1.5, some 1.48 but OD260/230 from 2.2-2.6. What happen with my samples ? How can i get higher OD260/280 ?. Thank so muck !!!
you have to get rid of traces of phenol and proteins!
Try another round of phenol:chloroform:isoamylalcohol extractions, this should improve your purity if it is that important for your downstream applications.
When doing Trizol extraction try not to get in contact with the interphase!
hey, sometimes column purification also helps....
Thanks so much.