Unstable A260/A230 in RNA isolation - (Mar/31/2011 )

I am using Qiagen RNeasy Mini kit isolate RNA form rats kidney, sometimes the RNA isolated has a low A260/A230(usually also low A260/A280) value because of the high 230 absorbtion. (wavelength scanning with Nanodrop(Thermo Fisher) shows a absorption peak near 230nm). I am sure it is not because of my disruption or homogenization problem because using the same homogenate did RNA extraction, sometimes got a low A260/A230 while sometimes absolutely normal. What's more, if the A230 high, then the A260 will be low. I want to know why and what can I do to solve this problem? Thanks!

Hongbo

hongbo on Thu Mar 31 21:21:46 2011 said:

Thanks a lot!~

Ahrenhase on Fri Apr 1 05:23:36 2011 said: