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Vector Related Discussions
  1. Plasmid dna size - (reply: 2)
  2. When overexpressing several genes using different vectors, what negative control - (reply: 2)
  3. pYr-CMV-Kan2 vector - (reply: 1)
  4. What is your favorite method for getting inserts into vectors? - (reply: 3)
  5. Using modified CFP and YFP plasmids for FRET assay - (reply: 1)
  6. GFP tagged plasmid - (reply: 1)
  7. Designing primers with his-tag for pET28a+ vector - (reply: 3)
  8. a half less of plasmid copy number in the solution resulting from ddPCR than qPC - (reply: 1)
  9. Plasmid purification for Gibson Assembly - (reply: 2)
  10. Co-transfection of multiple plasmids - (reply: 1)
  11. pKD1 based vector for K. lactis expression - (reply: 2)
  12. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
  13. what‘s phage display principle - (reply: 1)
  14. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
  15. pET32b+ vector issue - (reply: 1)
  16. Are relaxed plasmids origin of rep sensitive to bp substitution in RNA I/II sequ - (reply: 5)
  17. How stable is double digested plasmid - (reply: 7)
  18. Mature miRNA in expression vector? - (reply: 1)
  19. Ligation: insert & vector are about the same size - (reply: 3)
  20. low plasmid concentration - (reply: 1)
  21. What make a plasmid candidate for stable transfection - (reply: 1)
  22. Lower Plasmid Yields - (reply: 5)
  23. Cloning - Miniprep plasmid contaminated by gDNA? - (reply: 1)
  24. Question on plasmids - (reply: 4)
  25. *NO* DNA eluted from plasmid prep - Why? - (reply: 12)
  26. pUC and pSC101 vectors in the same cell - (reply: 3)
  27. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
  28. Antisense vectors confused about construction - (reply: 1)
  29. Double Digest pCD Vector - (reply: 3)
  30. Double digestion problem with PUCsp vector - (reply: 1)
  31. How much plasmid for transformation? - (reply: 8)
  32. replicating a mammalian expression vector in bacteria - (reply: 3)
  33. preparing a vector for transfection - (reply: 2)
  34. HELP: From Plasmid Vector to Bacterial Chromosome - (reply: 2)
  35. Suitable ratio of vector and insert in cloning - (reply: 5)
  36. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
  37. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
  38. plasmid repositories - (reply: 2)
  39. How to insert PreScission protease into a vector - (reply: 1)
  40. Why the insert become shorter after inserting into plasmid? - (reply: 2)
  41. real time qPCR standard range plasmid - (reply: 3)
  42. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
  43. Cloning a big insert in a small vector - (reply: 2)
  44. How to know the plasmid extracted are indeed the plasmid of interest? - (reply: 4)
  45. Plasmids recombining - (reply: 3)
  46. Plasmid Methylation - (reply: 3)
  47. Why are there two origin of replications in plasmids - (reply: 3)
  48. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
  49. Plasmid Amplification Issue - (reply: 6)
  50. ratios insert/vector - (reply: 3)
  51. Swapping promoter of destination vector not working. Please help! - (reply: 2)
  52. IRES in expression vector - (reply: 1)
  53. Why do we create own customised plasmid rather than buying iy? - (reply: 1)
  54. Can't isolate plasmid from cloned E.coli - (reply: 4)
  55. Denatured plasmid for pcr - (reply: 1)
  56. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
  57. Problems with M13 phage - (reply: 5)
  58. Screen for F plasmid in e. coli - (reply: 1)
  59. Plasmid Linearization Problems - (reply: 9)
  60. Changing the vector - (reply: 55)
  61. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
  62. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
  63. plasmid construction - (reply: 2)
  64. vector propagation in E coli - (reply: 1)
  65. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  66. Relationship between plasmid size and amount of lipofectamine needed? - (reply: 1)
  67. Can the pcDNA5/FRT/TO vector be used for transient overexpression? - (reply: 2)
  68. direction of the expression units in multi-gene vectors - (reply: 1)
  69. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
  70. ethanol precipitation plasmid DNA - (reply: 3)
  71. Plasmid storage conditions - (reply: 5)
  72. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
  73. Temperature sensitivity of Plasmids - (reply: 1)
  74. New (free) plasmid mapping program - (reply: 4)
  75. filling the digested vector and insert for blunt end cloning - (reply: 2)
  76. removing part from plasmid - (reply: 18)
  77. Help with plasmid extraction and redissolving? - (reply: 5)
  78. Nanodrop machine error message when measuring plasmid DNA concentration - (reply: 3)
  79. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  80. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
  81. Promoter sequence search in a plasmid map - (reply: 3)
  82. Heat vs pH during plasmid extraction - (reply: 8)
  83. Sterility issues with plasmid reconstitution buffer? - (reply: 3)
  84. Heating elution buffer for plasmid prep - (reply: 1)
  85. Cloning into pVIVO2 vector - (reply: 8)
  86. Cloning worked, but the expression vector is weird - (reply: 6)
  87. Using pBABE plasmids for regular transfection - (reply: 1)
  88. Rxn not working - amplify/linearize plasmid - (reply: 3)
  89. Cloning two genes in the same plasmid with one promotor - (reply: 4)
  90. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
  91. RNA contamination in plasmid extracted - (reply: 1)
  92. Problem in plasmid digestion after transformation - (reply: 4)
  93. Call for help! Plasmid extraction problem - (reply: 4)
  94. E. coli growing in LB-ampicillin medium without plasmid? Or miniprep problem? - (reply: 2)
  95. How can addgene sell plasmids with patented technology - (reply: 2)
  96. Transient transfection with large plasmid - (reply: 3)
  97. How to obtain Vectors used in articles - (reply: 5)
  98. Is it generally acceptable to ask another lab for cell line/plasmid/etc - (reply: 4)
  99. Gateway vectors - (reply: 3)
  100. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
  101. Plasmids DNA on filter paper - (reply: 1)
  102. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
  103. Kozak sequence and mammalian expression vector - (reply: 3)
  104. GFP vector - (reply: 1)
  105. Problem with Large vector & very small insert - (reply: 1)
  106. Storage life of plasmid DNA in water - (reply: 5)
  107. Insert & vector too dilute to perform ligation - (reply: 3)
  108. Plasmid DNA digestion problem - (reply: 3)
  109. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
  110. free online DNA & plasmid editing tool - (reply: 1)
  111. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
  112. Appearance of a salty plasmid preparation - (reply: 1)
  113. Common RE site on complementary strands in a Vector - (reply: 7)
  114. Transformation of Restricted plasmid - (reply: 3)
  115. Why is my insert missing from my plasmid? - (reply: 4)
  116. Plasmid digestion and gel purification using HindIII - (reply: 3)
  117. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
  118. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
  119. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  120. Colonies grown without plasmid - (reply: 7)
  121. Reconstituting plasmid DNA from blotting paper - water vs. TE buffer - (reply: 6)
  122. Plasmid on blotting paper - (reply: 1)
  123. Is it possible that the undigested version of this plasmid will be above the lin - (reply: 1)
  124. vector dephosphorylation - (reply: 3)
  125. What plasmid sizes do you work with for SDM? - (reply: 1)
  126. Running Plasmid DNA on Agarose Gel (Banding Pattern) - (reply: 1)
  127. Adshuttle vectors and adenoviruses - (reply: 1)
  128. Plasmid Purification Using an Alkaline Lysis - (reply: 1)
  129. cloning help; high salt level in vector - (reply: 4)
  130. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
  131. How to get rid of bacterial phage from a caterpillar surface? - (reply: 7)
  132. Running undigested plasmids on gel - (reply: 2)
  133. Plasmid isolation from Enterococcus - (reply: 1)
  134. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
  135. Software for vector sequence manipulation - (reply: 2)
  136. overexpress mature microRNA by siRNA vector - (reply: 2)
  137. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
  138. Whole plasmid amplification by PCR - (reply: 2)
  139. For promoter assay, which vector I should use? - (reply: 5)
  140. Sequencing of the cloned plasmid - (reply: 5)
  141. 4 way cloning into TA Vector - (reply: 10)
  142. Plasmid DNA contamination - (reply: 5)
  143. Problems with plasmid DNA extraction - (reply: 3)
  144. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
  145. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
  146. Can I quantify restricted plasmid and oligos? - (reply: 3)
  147. conditionally active plasmids - (reply: 1)
  148. Transient transfection of linearized plasmids - (reply: 3)
  149. Transfecting three plasmids into hES cells - (reply: 2)
  150. HUVEC plasmid transfection challenges - (reply: 2)
  151. Plasmid DNA - (reply: 3)
  152. Electroporate lentiviral vector for stable cell line - (reply: 2)
  153. Genomic DNA and plasmid DNA isolation - (reply: 3)
  154. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
  155. faint band after plasmid extraction - (reply: 7)
  156. pTRIPZ lentiviral vector induction - (reply: 1)
  157. pGEX-KG Vector insert in BL21 Codon Plus RP, Growth Difficulty - (reply: 4)
  158. plasmid extraction troubleshooting,please help !! - (reply: 9)
  159. plasmid digestion - problem with enzymes? - (reply: 4)
  160. Plasmid stability in Ecoli during storage - (reply: 3)
  161. which to use: miR or miRexpression vector - (reply: 1)
  162. Ligation with small insert and large vector - (reply: 6)
  163. Lost of Tet-on Vector - (reply: 3)
  164. Lentiviral 3rd gen transfer vector used with a 2nd generation packaging system - (reply: 1)
  165. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
  166. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
  167. Determining in frame in modified vector - (reply: 1)
  168. Creating primers to add restriction sites to vector backbone - (reply: 7)
  169. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
  170. Low copy vector for library generation - (reply: 2)
  171. Plasmid isolation from gram positive bacteria - (reply: 2)
  172. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
  173. problems with plasmid miniprep - (reply: 8)
  174. shRNA interference using Lenti virus vector - (reply: 1)
  175. Determining the amount of plasmid transfected - (reply: 1)
  176. How to check for the presence of plasmid? - (reply: 3)
  177. PCR with Plasmid recovered from filter paper - (reply: 6)
  178. Check contamination - how to sterilize vector - (reply: 1)
  179. Help for pGEM-T easy vector ligation problem - (reply: 2)
  180. pkp 59 vector - (reply: 2)
  181. Vector insert ratio in cloning - (reply: 3)
  182. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
  183. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
  184. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  185. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
  186. restricted PCR plasmid runs slower - (reply: 2)
  187. High frequency of vector religation - (reply: 10)
  188. Plasmid compatibly groups - (reply: 5)
  189. Plasmid isolation from agrobacterium - (reply: 1)
  190. Cutting my plasmid made it larger - (reply: 7)
  191. What are the "mammalian vectors N1 and C1"? - (reply: 1)
  192. Packaging vectors - (reply: 1)
  193. Recreate original plasmid by cutting out insert - (reply: 2)
  194. posting sequences of plasmid? - (reply: 3)
  195. Incomplete digestion of plasmid with single enzyme - (reply: 1)
  196. problem with plasmid digestion - (reply: 5)
  197. Protein Expression vector with small tag - (reply: 1)
  198. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
  199. unexpected band in PCR with plasmid - (reply: 4)
  200. Sequencing Primers and Plasmids - (reply: 3)
  201. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
  202. Replication of the Plasmid DNA - (reply: 6)
  203. expression plasmids for tRNA - (reply: 6)
  204. cloning pcr insert into plasmid - (reply: 2)
  205. Re-culturing empty plasmid - (reply: 1)
  206. microRNA promoter based expression vector? - (reply: 1)
  207. Can anyone recommend a good stable expression vector for human colorectal cancer - (reply: 1)
  208. help with a vector - (reply: 3)
  209. Adding T7 promoter sequence to vector - (reply: 2)
  210. Plasmid Miniprep - (reply: 2)
  211. Lipofectamin 2000 and Large Plasmid Transfection Optimization - (reply: 3)
  212. Tricistronic vector - (reply: 1)
  213. phage lysate purification - (reply: 2)
  214. Plasmid DNA at RT for a few days - (reply: 4)
  215. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
  216. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
  217. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
  218. Lentviral vectors - (reply: 1)
  219. plasmid rearrangement or sabotage - (reply: 2)
  220. pLDR20 Vector - (reply: 1)
  221. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  222. Linearising plasmid before transfection? - (reply: 2)
  223. Smear in plasmid sample - (reply: 9)
  224. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
  225. vector dephosphorylation - (reply: 5)
  226. Where can I purchase T2 or T4 phage genomic DNA? - (reply: 2)
  227. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
  228. cloning a gene in to a plasmid - (reply: 5)
  229. Characterizing an unknown plasmid - (reply: 6)
  230. Plasmid Purification and Digestion Problem - (reply: 2)
  231. PDCD4 Plasmid, RAW 264.7 not working Help - (reply: 2)
  232. QuikChange with Different plasmids - (reply: 1)
  233. Co-transfection with 2 plasmids - (reply: 10)
  234. Vector Digestion Problem - (reply: 4)
  235. Single Plasmid Gel Band - (reply: 1)
  236. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
  237. Transformation with a big plasmid - (reply: 4)
  238. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  239. Best ratio of 5 Plasmids for Lentivirus production - (reply: 4)
  240. digested plasmid - (reply: 2)
  241. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
  242. isolation of plasmid (midi and maxi prep) - (reply: 18)
  243. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
  244. How to clone insert with one sticky and one blunt end into vector? - (reply: 1)
  245. pUB Vector with two promoters - (reply: 6)
  246. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
  247. pkD46 vector growth in E.coli problem - (reply: 8)
  248. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
  249. Clean genomic DNA and plasmid DNA extraction from E.coli - (reply: 3)
  250. plasmid tetR and tet promoter - (reply: 2)
  251. Trouble in phage display - (reply: 1)
  252. Plasmid clone in BSP - (reply: 1)
  253. Swich selection in plasmid - (reply: 3)
  254. A good vector to make gene library? - (reply: 5)
  255. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
  256. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
  257. Hypothetical Synthetic Plasmid - (reply: 9)
  258. How many fragments in plasmid DNA prep? - (reply: 1)
  259. Problem with plasmids transformation - (reply: 6)
  260. Calcium chloride plasmid transfection - (reply: 1)
  261. How Much Plasmid? - (reply: 6)
  262. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
  263. Plasmid isolation - (reply: 1)
  264. plasmid;GI; ACESSION NUMBER - (reply: 2)
  265. virus transduction vs plasmid transfection - (reply: 4)
  266. How dangerous are non-viral vectors? - (reply: 3)
  267. having trouble getting insert ligation into vector - (reply: 3)
  268. Bizarre changes in plasmid band size - (reply: 3)
  269. where to get vector overexpressing caveolin-1? - (reply: 2)
  270. plasmid repository - (reply: 2)
  271. LZRS vector details - (reply: 1)
  272. transient transfection of retroviral vector without retroviral particle porudcti - (reply: 1)
  273. 12.4kb vector 6.7kb insert - (reply: 2)
  274. pGEM-T Easy vector contamination - (reply: 2)
  275. How to transfer His-tag from N- to C-terminus in plasmid - (reply: 1)
  276. High molecular plasmid isolation form kit and alkaline lysis - (reply: 2)
  277. Help: transduction of primary blood cells (T): lentiviral vs. adenoviral vector - (reply: 1)
  278. I need bynary vector pART27 - (reply: 1)
  279. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
  280. pCCL vector map - (reply: 2)
  281. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
  282. Pls help me with this plasmid!!! - (reply: 1)
  283. Standard curve using plasmid - (reply: 1)
  284. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  285. Methods of plasmid transformation? only heat shock? - (reply: 1)
  286. Cloning into inducible vector but no expression - (reply: 1)
  287. e coli vector and host selection - (reply: 1)
  288. No plasmid after a maxi prep mystery - (reply: 4)
  289. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
  290. Co-expression of 2 plasmids in E.coli - (reply: 1)
  291. How to avoid plasmid instability - (reply: 4)
  292. Plasmid vs cDNA amplification by PCR - (reply: 2)
  293. Shear-induced damage of plasmid by vortexing - (reply: 1)
  294. RNase Band in old plasmid stored at -40 - (reply: 2)
  295. Plasmid vector for yeast and E. coli - (reply: 2)
  296. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
  297. pGEX vector - (reply: 2)
  298. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  299. Filamentous phage infection - (reply: 2)
  300. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  301. how to find suitable vectors to transform wild-type bacteria - (reply: 10)
  302. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
  303. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  304. Plasmid digestion after transformation - (reply: 4)
  305. Blunt end ligation insert:vector ratio - (reply: 1)
  306. Smear above plasmid dna - (reply: 3)
  307. result of plasmid extraction - (reply: 2)
  308. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
  309. plasmid extraction - (reply: 5)
  310. agarose gel electrophoresis of plasmid extraction - (reply: 12)
  311. plasmid extraction - (reply: 3)
  312. Buy BAC vectors - (reply: 1)
  313. Is that a Plasmid??? - (reply: 5)
  314. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
  315. ask for a strain for the suicide vector called E. coli S17-1 pir - (reply: 1)
  316. plasmid extraction - (reply: 13)
  317. ligation colonies contain empty vector.. - (reply: 9)
  318. Is it posssible using shRNA vector to generate microRNA? - (reply: 2)
  319. Cloning two genes in the same plasmid - (reply: 4)
  320. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
  321. Best plasmid for GFP + Mammalian Selection - (reply: 1)
  322. Plasmid, gene orientation, insert orientation. - (reply: 1)
  323. Plasmid recovery problem - (reply: 3)
  324. Cloning problem using pET32a vector - (reply: 3)
  325. Electroporated plasmid loses the insert - (reply: 8)
  326. emty vector cloning - (reply: 3)
  327. Plasmid sequencing without primers - (reply: 1)
  328. RE site on complimentary strand of plasmid? - (reply: 1)
  329. How much of plasmid should be cut to see the gene - (reply: 1)
  330. Plasmid sequencing - (reply: 1)
  331. Plasmid isolation Qiagen - (reply: 2)
  332. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
  333. Is the T7 tag sequence immunogenic (from pET plasmid) - (reply: 2)
  334. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
  335. Help in RE digestion of vector - (reply: 5)
  336. Amplify fragment in plasmid? - (reply: 4)
  337. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
  338. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  339. Gateway cloning vector compatibility - (reply: 2)
  340. gene expression in E coli with PET21a vector - (reply: 1)
  341. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
  342. Template DNA (plasmid) concentration for PCR - (reply: 4)
  343. co-expression of 2 plasmids in E.coli - (reply: 3)
  344. How to choose the right plasmids for certain types of cell? - (reply: 1)
  345. Plasmid digestion create a bigger plasmid - (reply: 1)
  346. Cloning two fragments into a vector - (reply: 3)
  347. confirmation of insert in to vector - (reply: 3)
  348. whole plasmid pcr? - (reply: 6)
  349. Looking for a GFP plasmid (non-amp) - (reply: 3)
  350. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
  351. ligation of pcr product into expression vector - (reply: 4)
  352. amp plates containing xgal/iptg with expression vector - (reply: 6)
  353. Strange Vector Problems - (reply: 3)
  354. Insert lose in Phage display library - (reply: 6)
  355. extract plasmids from transfected eukaryotic cells - (reply: 6)
  356. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
  357. Lenti vector ligation - (reply: 2)
  358. Cloning from a pGEMT Easy vector - (reply: 3)
  359. Plasmid compatible with pBAD24 - (reply: 1)
  360. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
  361. COL E1 mutations in vector after sequencing - (reply: 1)
  362. Vector with inducible/repressible system - (reply: 1)
  363. Improving expression of recomb proteins in baculovirus - (reply: 1)
  364. Cloning genes into plant vector - (reply: 1)
  365. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
  366. Difference between plasmid DNA extraction and genomic DNA extraction - (reply: 3)
  367. Recombinant construct looks smaller than empty vector - (reply: 3)
  368. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
  369. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
  370. protein expression vector.. - (reply: 4)
  371. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
  372. Lentiviral Vector - Lentiviral Vector (reply: 5)
  373. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
  374. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
  375. Best transcription vector - (reply: 1)
  376. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
  377. which is the best plasmid vector for cloning BSP products? - (reply: 2)
  378. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
  379. Cloning into lentiviral vector - (reply: 11)
  380. use of pGEM -tT easy vectors - (reply: 2)
  381. ~200kb Plasmid DNA purification is not working - (reply: 1)
  382. ligating a 6 His tag to plasmid - (reply: 2)
  383. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
  384. Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
  385. expreesing cloned vector - having problems with texpreesing cloned gene in vector. (reply: 6)
  386. Plasmid sequencing failed why? - (reply: 10)
  387. Lactic Acid Bacteria - Challenges in working with LAB culturing, cloning and plasmid (reply: 7)
  388. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  389. SLIM mutagenesis of large plasmids - (reply: 8)
  390. Introduction to vector development - Basics on vectors types and construction (reply: 1)
  391. Can you receommend a vector? - (reply: 1)
  392. GFP vector as a transfectino efficiency indicator? - (reply: 3)
  393. why 12 h culture for plasmid prep? - (reply: 2)
  394. Big plasmids and Transfection - (reply: 3)
  395. How to add a T7 terminator to a vector? - (reply: 4)
  396. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
  397. Protein expression vectors Please help - (reply: 2)
  398. vectors please help - vectors cleavage sites (reply: 1)
  399. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
  400. very small insert and a very ling vector - (reply: 1)
  401. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
  402. help: why plasmid yield dropped ever since the first culture - (reply: 2)
  403. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
  404. pcr amplify an insert in plasmid? - (reply: 2)
  405. Single-digested vector shifts downward on gel - (reply: 1)
  406. odd plasmids - (reply: 2)
  407. Question about transduction of lentivirus vector into fibroblast ? - transduction (reply: 1)
  408. Lactobacillus plasmid - (reply: 1)
  409. Will transfected plasmids induce cytokine production? - (reply: 1)
  410. Question about plasmid midiprep - (reply: 1)
  411. Plasmid Miniprep yield problems - Low rate of yield using a Miniprep Kit (reply: 10)
  412. Mirror Image Phage Display - "special" contamination (reply: 9)
  413. pGL3 vector - (reply: 3)
  414. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
  415. plasmid isolation - sth lights up in the well of agarose gel (reply: 2)
  416. Overexpression plasmid is not expressing protein - (reply: 13)
  417. Secretion cloning vectors - (reply: 1)
  418. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
  419. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
  420. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
  421. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
  422. PCR problem - basic PCR for plasmid amplification (reply: 2)
  423. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
  424. New to Gene cloning - Plasmid vector preparation (reply: 2)
  425. cloning a 34kb insert - please suggest good vectors (reply: 1)
  426. HELP is needed for low expression using pEXP5-CT vector in BL21 (DE3) plyss - low expression after time course study with 1 mM IPTG (reply: 1)
  427. How long can I store cleaved/digested vector? - (reply: 9)
  428. why cut plasmid DNA with Hind111 and EcoR1? - (reply: 1)
  429. Low yields of plasmids from miniprep - (reply: 1)
  430. A gene is not inserted into the Plasmid - (reply: 2)
  431. purification of PCR product for cloning in a vector - (reply: 3)
  432. Phage Contamination of Cultures? - Phage contamination or somethign else? (reply: 3)
  433. Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
  434. Plasmid Question - (reply: 4)
  435. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
  436. Weird migration of double digested plasmid - (reply: 4)
  437. pMX-PURO Vector not growing - (reply: 3)
  438. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  439. Help for co-transfection of proteins and plasmid - (reply: 1)
  440. subcloning into bacterial expression vector - (reply: 1)
  441. Calculating MOI of viral vector - (reply: 1)
  442. cloning into F1 ori of expression vector??? - (reply: 1)
  443. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
  444. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  445. How to store plasmid DNA on filter paper - I want to make a folder of all of them (reply: 3)
  446. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
  447. Separating cut/non-cut vector - (reply: 6)
  448. ligating small inert into a large vector - (reply: 4)
  449. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
  450. Really strange problem with sequencing and plasmid purification! - (reply: 8)
  451. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  452. plasmid DNA versus genomic DNA - concentrations (reply: 1)
  453. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  454. Undigestable/unrestrictable Plasmid? - (reply: 1)
  455. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  456. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  457. metagenomic library consturction - BAC vectors (reply: 1)
  458. Problem in stable expression of target protein using lentiviral vector - (reply: 3)
  459. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
  460. Suitable vector backbone for in vitro translation in Dros.embryos - (reply: 1)
  461. Viruses and plasmid gaining resistance toward drugs can lead to speciatio - (reply: 3)
  462. pGEX vectors and GST expression - (reply: 3)
  463. problems encountered when working on a large plasmid - (reply: 2)
  464. expression vector with built in promoter - (reply: 1)
  465. make a Mega plasmid mutation - (reply: 1)
  466. Anyone make their own PCR cloning vector? - (reply: 5)
  467. Bicistronic vector vs "normal" vector - (reply: 5)
  468. Can't seem to get insert into vector... please help - (reply: 4)
  469. plasmid cloning - i need help.. can anyone answer me (reply: 5)
  470. Plasmid standard problem - (reply: 2)
  471. Do plasmids work in bacteria? - (reply: 1)
  472. CEN sequences on plasmids (yeast) - (reply: 4)
  473. phage DNA - DNA wont migrate on agarose gel (reply: 3)
  474. Plasmid out of BL21 for mutagensis - (reply: 3)
  475. ORI names - name of a plasmid ori (reply: 11)
  476. uncut vector contamination in gel purified band - (reply: 2)
  477. lentivirus - possible to use 2 transfer vectors? (reply: 1)
  478. Change origin of a plasmid - (reply: 4)
  479. Better to sequence from plasmid? - (reply: 5)
  480. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  481. Does a single colony of E.coli contain more than one plasmid - (reply: 7)
  482. pGL3 promoter vector - (reply: 1)
  483. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  484. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
  485. DNA extraction from induced phage - (reply: 1)
  486. How to get rid of phage contamination - (reply: 1)
  487. lambda phage library - (reply: 4)
  488. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
  489. DNA isolation from plant nuclei by plasmid extraction kit - (reply: 1)
  490. problem with pLK01 plasmid ligation - (reply: 2)
  491. Cloning long fragment...got lots of colonies with vectors that have no inserts.. - (reply: 9)
  492. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  493. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
  494. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
  495. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  496. Dephosphorylation of vector - (reply: 8)
  497. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
  498. Can a single colony contains more than 2 types of insert in their plasmids? - (reply: 2)
  499. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
  500. PCR mutagenesis of plasmid - (reply: 5)
  501. Guanadine HCl for plasmid miniprep - (reply: 3)
  502. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
  503. Large vector cloning-recombination problem? - (reply: 1)
  504. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
  505. Not getting plasmid from miniprep - (reply: 7)
  506. Need advice on cloning very small insert into a vector - (reply: 3)
  507. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
  508. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
  509. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
  510. Digesting large amount of plasmid - (reply: 4)
  511. Vectors with special MCS - (reply: 5)
  512. Cloning <100 bp fragments into a vector - (reply: 7)
  513. Cloning <100 bp fragments into a plasmid - (reply: 7)
  514. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
  515. Help! my plasmid size changed after ligation - (reply: 5)
  516. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
  517. Restriction digestion of gDNA for phage library - (reply: 5)
  518. mammalian expression vector - (reply: 2)
  519. Electroporation doesn't like my plasmid??? - (reply: 4)
  520. Problem with ligation - Insert into the "wrong" vector (reply: 11)
  521. Cloning in pCR2.1 plasmid - (reply: 7)
  522. No expresion of transgene - Retroviral vector (reply: 1)
  523. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  524. lambda phage library - (reply: 1)
  525. More colonies fron vector only control? - (reply: 1)
  526. empty vectors more than the insert - (reply: 6)
  527. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
  528. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
  529. phage display - peptide differentiation (reply: 1)
  530. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  531. ligation of an adaptor into a vector - (reply: 4)
  532. Rnase A for plasmid miniprep - cheaper alternative to qiagen? (reply: 4)
  533. vectors and his-tags - (reply: 3)
  534. miRNA 3´UTR target expression vectors - (reply: 6)
  535. Help cloning large insert into large vector - (reply: 7)
  536. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
  537. problem about vector Dephosphorylation - (reply: 2)
  538. Cloning: Randomly recombined Plasmids? - (reply: 6)
  539. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
  540. plasmid + sepharose - how do this work? (reply: 1)
  541. Transformation and ligation problem - Trasnformation of ligation sample shows only vector size.. (reply: 3)
  542. Anyone uses plasmid to make standard curves? how to make adjustment b/w plates - real time (reply: 4)
  543. How to elute plasmid dried on paper? - I just received plasmid samples by mail (reply: 4)
  544. are vacuum manifolds (plasmid prep) interchange with brands? - (reply: 2)
  545. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  546. Plasmid size problems! - (reply: 3)
  547. Left DNA plasmid out for a week - (reply: 3)
  548. Vector maps - (reply: 3)
  549. Transfecting primary cells with plasmid - (reply: 1)
  550. question about plasmid extraction - (reply: 2)
  551. Ligating 2 Linear fragments into a vector at Once - (reply: 4)
  552. problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
  553. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
  554. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
  555. Disappearing plasmid - (reply: 5)
  556. Transferring Plasmid from Aerobic to Anaerobic Bacteria - a thought about the oil eating microbes (OEMs) (reply: 1)
  557. Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
  558. problem in plasmid transfection - (reply: 2)
  559. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
  560. Plasmid DNA mini-prep - no good isolation (reply: 5)
  561. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
  562. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
  563. plasmid extraction - why dilute (reply: 3)
  564. Suicide vector - (reply: 2)
  565. very high molecular weight plasmid - (reply: 6)
  566. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  567. vectors maps - (reply: 2)
  568. How to dissolve phage library dry powder - (reply: 2)
  569. Fake positive colony in lamda RED reconbination, why? - (reply: 2)
  570. Fake positive colony in lamda RED reconbination, why? - (reply: 3)
  571. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
  572. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  573. Concepts behind plasmid prep - (reply: 2)
  574. help with pCMV-myc vector - (reply: 2)
  575. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
  576. PCR off plasmid for screening - (reply: 2)
  577. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
  578. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  579. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  580. Vector frameshift problem - (reply: 3)
  581. Plasmid frameshift problem after clonning - (reply: 2)
  582. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
  583. plasmid extraction kit wash buffer solution - (reply: 3)
  584. plasmid extraction kit solution composition - wash buffer (reply: 2)
  585. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
  586. are plasmids used for lab work methylated - (reply: 1)
  587. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  588. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  589. Isolation of protein crosslinked to plasmid - (reply: 3)
  590. Inseparable insert in gel purification of the vector - (reply: 3)
  591. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  592. knockdown issue...please help... - shRNA pGIPZ vector (reply: 1)
  593. Which Plasmid DNA for standard curve? - (reply: 5)
  594. pRL-TK as primary Vector ? - (reply: 1)
  595. Ligated Vector Age - When is a ligated vector too old to use? (reply: 1)
  596. GFP insert - trying to insert GFP in a vector (reply: 1)
  597. Plasmid problem - From PCR product (reply: 7)
  598. IFU, MOI, lentiviral vectors - (reply: 1)
  599. viral vector - retroviral vectors (reply: 1)
  600. Gateway and Entry Vectors. - (reply: 1)
  601. Checking PCR insert (into pGEMT vector) - (reply: 2)
  602. Transfection - Plasmid Uptake Control - (reply: 2)
  603. info about the little p in pH - i kept my promise phage!!! (reply: 4)
  604. Culture Grow well but No Plasmid - (reply: 2)
  605. software for plasmid drawing - (reply: 13)
  606. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
  607. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
  608. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
  609. plasmid purification kit for transfection - (reply: 2)
  610. best plasmid purification kit for transfection - (reply: 1)
  611. Expression problem using invitrogen pRSET C vector - (reply: 5)
  612. Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
  613. Which pGL4 vector to choose - (reply: 2)
  614. Vector containing N-his tag and TEV - (reply: 1)
  615. LR reaction - Cloning into a expression vector (reply: 1)
  616. Vector map for pMH1 - Can't find it anywhere! (reply: 2)
  617. Checking Plasmid Expression - (reply: 1)
  618. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  619. about pre-miRNA cloned into lentivirus vectors - (reply: 4)
  620. qPCR determination of phage numbers - slope difference for different phage clones (reply: 2)
  621. PCR a plasmid protein - (reply: 2)
  622. plasmid DNA disappear in the minipre - (reply: 2)
  623. Interpreting a Plasmid Profile gel photo - (reply: 3)
  624. AdEasy Vector System: Why PacI digestion? - (reply: 1)
  625. marker swap plasmid - (reply: 4)
  626. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
  627. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
  628. plasmid dna isolation - (reply: 1)
  629. Vector needed - (reply: 1)
  630. having the wrong vector in my clone - (reply: 3)
  631. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  632. siRNA and plasmid cotransfection - (reply: 1)
  633. rna from plasmid - (reply: 1)
  634. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
  635. Problems facing in cloning with pBI121 vector - (reply: 1)
  636. design parameters for multiple siRNA expression in vector - (reply: 4)
  637. Plasmid curing method - (reply: 4)
  638. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  639. Co-transfection of miRNA and vector expressing target sequence - (reply: 2)
  640. religate the vector - need good protocol! - (reply: 4)
  641. plasmid DNA lab question - (reply: 11)
  642. ShRNA vectors for other than mammalian systems - shRNA for S. pombe (reply: 6)
  643. Problem amplifying plasmid - (reply: 1)
  644. Can linear vectors be transformed and can they express resistance genes? - (reply: 4)
  645. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
  646. Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)
  647. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
  648. HELP! Can't double digest pcDNA3.1 vector - (reply: 6)
  649. Help complementing my mutant in trans - help me find a plasmid (reply: 1)
  650. What about the Baculovirus expression system? - (reply: 2)
  651. Vector NTI from Invitrogen - (reply: 4)
  652. role of glucose in plasmid isolation? - (reply: 1)
  653. Plasmids for protein expression in mammalian cells - (reply: 2)
  654. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
  655. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
  656. Amplfy vector - How to amplify vector (reply: 2)
  657. after re-isolation of vector, get single band - (reply: 4)
  658. Cloning big fragment into smaller vector - (reply: 3)
  659. from where can i get the P1 phage - (reply: 1)
  660. Ligation/Transformation problems & 260/230 ratio of vector - (reply: 4)
  661. plasmid, BAC, or cosmid? - (reply: 3)
  662. Ligation of digested plasmid to hairpin oligo - (reply: 5)
  663. Nucleospin Extract II kit also for plasmids? - (reply: 5)
  664. Best plasmid miniprep? - (reply: 13)
  665. Plasmid instability in E.coli BL21? - (reply: 3)
  666. Difficulty cloning vector with multiple Lox sites - (reply: 2)
  667. Working gene-coding plasmid does not produce transcript or protein - (reply: 2)
  668. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
  669. Vector sequence included in (PCR) amplified insert - (reply: 2)
  670. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
  671. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  672. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
  673. Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL - (reply: 1)
  674. pCLBABE puro retriviral vector - (reply: 1)
  675. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
  676. Plasmid DNA sequence and map - PLASMID DNA (reply: 2)
  677. Plasmid linearization by PCR - (reply: 4)
  678. plasmid sequence identification problem - (reply: 2)
  679. Best lentiviral vector - (reply: 2)
  680. Suitable bacterial reporter vectors - (reply: 3)
  681. plasmid construct problem - (reply: 3)
  682. Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
  683. Transformation problem. Please help! - 1.7kb insert to 5.4kb vector; double digested w/ EcoRV & XhoI (reply: 17)
  684. Transient expression vector - (reply: 11)
  685. How to get plasmid maps from publications? - (reply: 5)
  686. pMSP12-GFP map? - How to get plasmid maps. (reply: 1)
  687. Salmonella vector- non-ColE1 - Suggestion for good Salmonella vector? (reply: 7)
  688. A gateway cloning expression vector question - (reply: 1)
  689. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
  690. File format for plasmids in Vector NTI, Clone Manager etc - I'd like a compatible format with no/minimal data loss (reply: 3)
  691. Ligation Failed, too little Vector??? - Ligation trouble shooting (reply: 5)
  692. Low level of expression - in pASK-IBA7 vector (reply: 6)
  693. How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
  694. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  695. Choice of expression vector - how to choose an expression vector (reply: 1)
  696. New Plasmid Draw/Plotter Program (Free) - (reply: 4)
  697. Plasmid DNA isolation - neutralisation of DNA (reply: 5)
  698. Why the fluoresence is dim or dispear in miRNA vector? - miRNA (reply: 3)
  699. Phage removal of E.coli - (reply: 8)
  700. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
  701. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
  702. Luciferase reporter induced by betagal vector?? - (reply: 1)
  703. PCR problem from transformed TOPO TA vector - (reply: 3)
  704. Struggling for months with one little ligation/transformation - please help! - XhoI digested 1,5 kb insert needs to be ligated into 4,8kb vector (reply: 11)
  705. Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
  706. Self-Ligation of digested vector - (reply: 2)
  707. Ligation issue: Does vector size matter? - (reply: 10)
  708. Plasmid sequence - (reply: 1)
  709. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
  710. Promoters and vectors for shRNA? The need for specific vectors and promoters? - (reply: 4)
  711. Mammalian expression vectors with HA, c-myc, FLAG or anyother tag - The lab is running out of funds and need some cheap source. (reply: 3)
  712. What is stringent vector? - Is it related to level of expression? (reply: 2)
  713. expression vector in E. coli - the best expression vector in E. coli (reply: 5)
  714. pBMN-GFP as episomal vector - (reply: 1)
  715. where can i ontain the binary vector pCB301? - (reply: 1)
  716. plasmid isolation - (reply: 4)
  717. Plasmid supercoiling affecting PCR? - (reply: 2)
  718. Miniprep / plasmid question - How long can you get plasmid? (reply: 5)
  719. TOP10F' - Can they be used for regular vector propagation? (reply: 1)
  720. Cloning done....sequence of the insert for the expression vector - (reply: 1)
  721. heterodimer - bicistronic vector? (reply: 2)
  722. looking for a vector with a Neo cassette, HindIII and BamHI restriction sites (i - (reply: 7)
  723. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  724. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
  725. problem with plasmid extraction - (reply: 3)
  726. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
  727. How to transfect two plasmids in a cell - (reply: 10)
  728. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
  729. How to separate large size plasmids ~12kb - (reply: 8)
  730. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  731. Vector NTI users!!!! please read this!!! - free alternative to vector nti (reply: 10)
  732. low plasmid yield from midi preps - (reply: 4)
  733. Plasmid isolation, help with dilution step? - (reply: 7)
  734. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
  735. full digestion of plasmid to get only dNTPs - (reply: 3)
  736. Lenti viral Vector with GFP - (reply: 4)
  737. inducible shRNA vector system - (reply: 7)
  738. size limit of plasmid origins - (reply: 2)
  739. Creating empty vector by self-ligation - (reply: 31)
  740. How to increase the concentration insert or vector or no cut plasmid - How to increase the concentration insert or vector or no cut plasmid (reply: 4)
  741. confusion with different forms of plasmid - (reply: 3)
  742. pQE vector protein expression - (reply: 3)
  743. zebrafish gene regulation can I use mammel expression vector? - (reply: 1)
  744. Plasmid as standard - Plasmid calculation (reply: 6)
  745. Toxic Plasmid?? Toxic Insert?? - (reply: 2)
  746. ligation problem with SalI degested vector and insert - (reply: 6)
  747. Effect of EtBr on plasmid supercoiling - (reply: 5)
  748. Quantifying plasmid DNA - Nanodrop and Qubit (Invitrogen) suck! (reply: 2)
  749. lentivirus vector problem--help help - (reply: 6)
  750. How to know the exact plasmid size? - - supercoiled, nicked, linear (reply: 6)
  751. Digestion of a plasmid - (reply: 1)
  752. no colonies - Cloning using pENTR vector, no colonies (reply: 2)
  753. Please help Cloning issues with smaI and pUNK1 vector - (reply: 2)
  754. Retroviral vector with luciferase - (reply: 1)
  755. Problems cloning Hairpin vector - (reply: 3)
  756. Sequences of generally used plasmids? - Database for sequences available? (reply: 7)
  757. plasmid copy number - method for determination of plasmid copy number (reply: 2)
  758. Purfiying Unmethylated Plasmid - unmethylated plasmid (reply: 3)
  759. Amplifying plasmid - non specific binding of primer - (reply: 3)
  760. Storing plasmid at 4 degrees C - (reply: 5)
  761. Program to draw plasmids - draw plasmids (reply: 9)
  762. Transfer of gene from one vector to another vector - (reply: 2)
  763. Transfection of vectors - (reply: 8)
  764. CIP required for double digestion of plasmid? - (reply: 3)
  765. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
  766. Plasmid replication - Plasmid replication (reply: 13)
  767. Help with cloning a plasmid that contains repeats! - (reply: 2)
  768. Plasmid extraction sans commercial kits - (reply: 3)
  769. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
  770. Myc Tag Vector - (reply: 1)
  771. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  772. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
  773. mutations in plasmids - (reply: 3)
  774. Gel purification of vector DNA - low yield - (reply: 26)
  775. Sequential restriction digestion of plasmid - (reply: 58)
  776. Problem cloning oligo into double digested vector - (reply: 9)
  777. Big insert to put back into the vector - (reply: 17)
  778. plasmid spin column usage - (reply: 5)
  779. deleting lacZ gene from control vector - (reply: 5)
  780. Vector for conditional knockout - (reply: 1)
  781. What is the biggest insert for a viral vector? - (reply: 15)
  782. Stored bacteria lost plasmids? - (reply: 12)
  783. difference between a linear and circular plasmid/DNA for stable transfections - will a circular plasmid stably integrate into the genome? (reply: 4)
  784. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
  785. cloning problems - empty vector (reply: 3)
  786. Selection during Extraction: Plasmid or Genomic DNA? - (reply: 1)
  787. Anyone have some pEGFP-N1 or N3 vectors? - (reply: 2)
  788. PLASMID ISOLATION - (reply: 10)
  789. Ligation very large vector and small insert - It will not work... (reply: 7)
  790. Frustrated with Plasmid Preps - need help! - (reply: 14)
  791. Can't find the sequences of vector pIJ 702 - Help (reply: 1)
  792. In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)