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Vector Related Discussions
  1. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
  2. Plasmid Amplification Issue - (reply: 6)
  3. ratios insert/vector - (reply: 3)
  4. Swapping promoter of destination vector not working. Please help! - (reply: 2)
  5. IRES in expression vector - (reply: 1)
  6. Can't isolate plasmid from cloned E.coli - (reply: 3)
  7. Denatured plasmid for pcr - (reply: 1)
  8. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
  9. Problems with M13 phage - (reply: 5)
  10. Screen for F plasmid in e. coli - (reply: 1)
  11. Plasmid Linearization Problems - (reply: 9)
  12. Changing the vector - (reply: 55)
  13. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
  14. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
  15. plasmid construction - (reply: 2)
  16. vector propagation in E coli - (reply: 1)
  17. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  18. Relationship between plasmid size and amount of lipofectamine needed? - (reply: 1)
  19. Can the pcDNA5/FRT/TO vector be used for transient overexpression? - (reply: 2)
  20. direction of the expression units in multi-gene vectors - (reply: 1)
  21. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
  22. ethanol precipitation plasmid DNA - (reply: 3)
  23. Plasmid storage conditions - (reply: 3)
  24. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
  25. Temperature sensitivity of Plasmids - (reply: 1)
  26. New (free) plasmid mapping program - (reply: 4)
  27. filling the digested vector and insert for blunt end cloning - (reply: 2)
  28. removing part from plasmid - (reply: 18)
  29. Help with plasmid extraction and redissolving? - (reply: 5)
  30. Nanodrop machine error message when measuring plasmid DNA concentration - (reply: 3)
  31. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  32. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
  33. Promoter sequence search in a plasmid map - (reply: 3)
  34. Heat vs pH during plasmid extraction - (reply: 8)
  35. Sterility issues with plasmid reconstitution buffer? - (reply: 3)
  36. Heating elution buffer for plasmid prep - (reply: 1)
  37. Cloning into pVIVO2 vector - (reply: 8)
  38. Cloning worked, but the expression vector is weird - (reply: 6)
  39. Using pBABE plasmids for regular transfection - (reply: 1)
  40. Rxn not working - amplify/linearize plasmid - (reply: 3)
  41. Cloning two genes in the same plasmid with one promotor - (reply: 4)
  42. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
  43. RNA contamination in plasmid extracted - (reply: 1)
  44. Problem in plasmid digestion after transformation - (reply: 4)
  45. Call for help! Plasmid extraction problem - (reply: 4)
  46. E. coli growing in LB-ampicillin medium without plasmid? Or miniprep problem? - (reply: 2)
  47. How can addgene sell plasmids with patented technology - (reply: 2)
  48. Transient transfection with large plasmid - (reply: 3)
  49. How to obtain Vectors used in articles - (reply: 5)
  50. Is it generally acceptable to ask another lab for cell line/plasmid/etc - (reply: 4)
  51. Gateway vectors - (reply: 3)
  52. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
  53. Plasmids DNA on filter paper - (reply: 1)
  54. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
  55. Kozak sequence and mammalian expression vector - (reply: 3)
  56. GFP vector - (reply: 1)
  57. Problem with Large vector & very small insert - (reply: 1)
  58. Storage life of plasmid DNA in water - (reply: 5)
  59. Insert & vector too dilute to perform ligation - (reply: 3)
  60. Plasmid DNA digestion problem - (reply: 3)
  61. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
  62. free online DNA & plasmid editing tool - (reply: 1)
  63. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
  64. Appearance of a salty plasmid preparation - (reply: 1)
  65. Common RE site on complementary strands in a Vector - (reply: 7)
  66. Transformation of Restricted plasmid - (reply: 3)
  67. Why is my insert missing from my plasmid? - (reply: 4)
  68. Plasmid digestion and gel purification using HindIII - (reply: 3)
  69. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
  70. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
  71. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  72. Colonies grown without plasmid - (reply: 7)
  73. Reconstituting plasmid DNA from blotting paper - water vs. TE buffer - (reply: 6)
  74. Plasmid on blotting paper - (reply: 1)
  75. Is it possible that the undigested version of this plasmid will be above the lin - (reply: 1)
  76. vector dephosphorylation - (reply: 3)
  77. What plasmid sizes do you work with for SDM? - (reply: 1)
  78. Running Plasmid DNA on Agarose Gel (Banding Pattern) - (reply: 1)
  79. Adshuttle vectors and adenoviruses - (reply: 1)
  80. Plasmid Purification Using an Alkaline Lysis - (reply: 1)
  81. cloning help; high salt level in vector - (reply: 4)
  82. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
  83. How to get rid of bacterial phage from a caterpillar surface? - (reply: 7)
  84. Running undigested plasmids on gel - (reply: 2)
  85. Plasmid isolation from Enterococcus - (reply: 1)
  86. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
  87. Software for vector sequence manipulation - (reply: 2)
  88. overexpress mature microRNA by siRNA vector - (reply: 2)
  89. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
  90. Whole plasmid amplification by PCR - (reply: 2)
  91. For promoter assay, which vector I should use? - (reply: 5)
  92. Sequencing of the cloned plasmid - (reply: 5)
  93. 4 way cloning into TA Vector - (reply: 10)
  94. Plasmid DNA contamination - (reply: 5)
  95. Problems with plasmid DNA extraction - (reply: 3)
  96. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
  97. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
  98. Can I quantify restricted plasmid and oligos? - (reply: 3)
  99. conditionally active plasmids - (reply: 1)
  100. Transient transfection of linearized plasmids - (reply: 3)
  101. Transfecting three plasmids into hES cells - (reply: 2)
  102. HUVEC plasmid transfection challenges - (reply: 2)
  103. Plasmid DNA - (reply: 3)
  104. Electroporate lentiviral vector for stable cell line - (reply: 2)
  105. Genomic DNA and plasmid DNA isolation - (reply: 3)
  106. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
  107. faint band after plasmid extraction - (reply: 7)
  108. pTRIPZ lentiviral vector induction - (reply: 1)
  109. pGEX-KG Vector insert in BL21 Codon Plus RP, Growth Difficulty - (reply: 4)
  110. plasmid extraction troubleshooting,please help !! - (reply: 9)
  111. plasmid digestion - problem with enzymes? - (reply: 4)
  112. Plasmid stability in Ecoli during storage - (reply: 3)
  113. which to use: miR or miRexpression vector - (reply: 1)
  114. Ligation with small insert and large vector - (reply: 6)
  115. Lost of Tet-on Vector - (reply: 3)
  116. Lentiviral 3rd gen transfer vector used with a 2nd generation packaging system - (reply: 1)
  117. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
  118. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
  119. Determining in frame in modified vector - (reply: 1)
  120. Creating primers to add restriction sites to vector backbone - (reply: 7)
  121. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
  122. Low copy vector for library generation - (reply: 2)
  123. Plasmid isolation from gram positive bacteria - (reply: 2)
  124. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
  125. problems with plasmid miniprep - (reply: 8)
  126. shRNA interference using Lenti virus vector - (reply: 1)
  127. Determining the amount of plasmid transfected - (reply: 1)
  128. How to check for the presence of plasmid? - (reply: 3)
  129. PCR with Plasmid recovered from filter paper - (reply: 6)
  130. Check contamination - how to sterilize vector - (reply: 1)
  131. Help for pGEM-T easy vector ligation problem - (reply: 2)
  132. pkp 59 vector - (reply: 2)
  133. Vector insert ratio in cloning - (reply: 3)
  134. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
  135. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
  136. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  137. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
  138. restricted PCR plasmid runs slower - (reply: 2)
  139. High frequency of vector religation - (reply: 10)
  140. Plasmid compatibly groups - (reply: 5)
  141. Plasmid isolation from agrobacterium - (reply: 1)
  142. Cutting my plasmid made it larger - (reply: 7)
  143. What are the "mammalian vectors N1 and C1"? - (reply: 1)
  144. Packaging vectors - (reply: 1)
  145. Recreate original plasmid by cutting out insert - (reply: 2)
  146. posting sequences of plasmid? - (reply: 3)
  147. Incomplete digestion of plasmid with single enzyme - (reply: 1)
  148. problem with plasmid digestion - (reply: 5)
  149. Protein Expression vector with small tag - (reply: 1)
  150. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
  151. unexpected band in PCR with plasmid - (reply: 4)
  152. Sequencing Primers and Plasmids - (reply: 3)
  153. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
  154. Replication of the Plasmid DNA - (reply: 6)
  155. expression plasmids for tRNA - (reply: 5)
  156. cloning pcr insert into plasmid - (reply: 2)
  157. Re-culturing empty plasmid - (reply: 1)
  158. microRNA promoter based expression vector? - (reply: 1)
  159. Can anyone recommend a good stable expression vector for human colorectal cancer - (reply: 1)
  160. help with a vector - (reply: 3)
  161. Adding T7 promoter sequence to vector - (reply: 2)
  162. Plasmid Miniprep - (reply: 2)
  163. Lipofectamin 2000 and Large Plasmid Transfection Optimization - (reply: 3)
  164. Tricistronic vector - (reply: 1)
  165. phage lysate purification - (reply: 2)
  166. Plasmid DNA at RT for a few days - (reply: 4)
  167. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
  168. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
  169. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
  170. plasmid rearrangement or sabotage - (reply: 2)
  171. pLDR20 Vector - (reply: 1)
  172. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  173. Linearising plasmid before transfection? - (reply: 2)
  174. Smear in plasmid sample - (reply: 9)
  175. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
  176. vector dephosphorylation - (reply: 5)
  177. Where can I purchase T2 or T4 phage genomic DNA? - (reply: 2)
  178. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
  179. cloning a gene in to a plasmid - (reply: 5)
  180. Characterizing an unknown plasmid - (reply: 6)
  181. Plasmid Purification and Digestion Problem - (reply: 2)
  182. PDCD4 Plasmid, RAW 264.7 not working Help - (reply: 2)
  183. QuikChange with Different plasmids - (reply: 1)
  184. Co-transfection with 2 plasmids - (reply: 10)
  185. Vector Digestion Problem - (reply: 4)
  186. Single Plasmid Gel Band - (reply: 1)
  187. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
  188. Transformation with a big plasmid - (reply: 4)
  189. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  190. Best ratio of 5 Plasmids for Lentivirus production - (reply: 4)
  191. digested plasmid - (reply: 2)
  192. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
  193. isolation of plasmid (midi and maxi prep) - (reply: 18)
  194. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
  195. How to clone insert with one sticky and one blunt end into vector? - (reply: 1)
  196. pUB Vector with two promoters - (reply: 6)
  197. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
  198. pkD46 vector growth in E.coli problem - (reply: 8)
  199. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
  200. Clean genomic DNA and plasmid DNA extraction from E.coli - (reply: 3)
  201. plasmid tetR and tet promoter - (reply: 2)
  202. Plasmid clone in BSP - (reply: 1)
  203. Swich selection in plasmid - (reply: 3)
  204. A good vector to make gene library? - (reply: 5)
  205. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
  206. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
  207. Hypothetical Synthetic Plasmid - (reply: 9)
  208. How many fragments in plasmid DNA prep? - (reply: 1)
  209. Problem with plasmids transformation - (reply: 6)
  210. Calcium chloride plasmid transfection - (reply: 1)
  211. How Much Plasmid? - (reply: 6)
  212. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
  213. Plasmid isolation - (reply: 1)
  214. plasmid;GI; ACESSION NUMBER - (reply: 2)
  215. virus transduction vs plasmid transfection - (reply: 4)
  216. How dangerous are non-viral vectors? - (reply: 3)
  217. having trouble getting insert ligation into vector - (reply: 3)
  218. Bizarre changes in plasmid band size - (reply: 3)
  219. where to get vector overexpressing caveolin-1? - (reply: 2)
  220. plasmid repository - (reply: 2)
  221. LZRS vector details - (reply: 1)
  222. transient transfection of retroviral vector without retroviral particle porudcti - (reply: 1)
  223. 12.4kb vector 6.7kb insert - (reply: 2)
  224. pGEM-T Easy vector contamination - (reply: 2)
  225. How to transfer His-tag from N- to C-terminus in plasmid - (reply: 1)
  226. High molecular plasmid isolation form kit and alkaline lysis - (reply: 2)
  227. Help: transduction of primary blood cells (T): lentiviral vs. adenoviral vector - (reply: 1)
  228. I need bynary vector pART27 - (reply: 1)
  229. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
  230. pCCL vector map - (reply: 2)
  231. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
  232. Pls help me with this plasmid!!! - (reply: 1)
  233. Standard curve using plasmid - (reply: 1)
  234. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  235. Methods of plasmid transformation? only heat shock? - (reply: 1)
  236. Cloning into inducible vector but no expression - (reply: 1)
  237. e coli vector and host selection - (reply: 1)
  238. No plasmid after a maxi prep mystery - (reply: 4)
  239. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
  240. Co-expression of 2 plasmids in E.coli - (reply: 1)
  241. How to avoid plasmid instability - (reply: 4)
  242. Plasmid vs cDNA amplification by PCR - (reply: 2)
  243. Shear-induced damage of plasmid by vortexing - (reply: 1)
  244. RNase Band in old plasmid stored at -40 - (reply: 2)
  245. Plasmid vector for yeast and E. coli - (reply: 2)
  246. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
  247. pGEX vector - (reply: 2)
  248. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  249. Filamentous phage infection - (reply: 2)
  250. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  251. how to find suitable vectors to transform wild-type bacteria - (reply: 10)
  252. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
  253. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  254. Plasmid digestion after transformation - (reply: 4)
  255. Blunt end ligation insert:vector ratio - (reply: 1)
  256. Smear above plasmid dna - (reply: 3)
  257. result of plasmid extraction - (reply: 2)
  258. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
  259. plasmid extraction - (reply: 5)
  260. agarose gel electrophoresis of plasmid extraction - (reply: 12)
  261. plasmid extraction - (reply: 3)
  262. Buy BAC vectors - (reply: 1)
  263. Is that a Plasmid??? - (reply: 5)
  264. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
  265. ask for a strain for the suicide vector called E. coli S17-1 pir - (reply: 1)
  266. plasmid extraction - (reply: 13)
  267. ligation colonies contain empty vector.. - (reply: 9)
  268. Is it posssible using shRNA vector to generate microRNA? - (reply: 2)
  269. Cloning two genes in the same plasmid - (reply: 4)
  270. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
  271. Best plasmid for GFP + Mammalian Selection - (reply: 1)
  272. Plasmid, gene orientation, insert orientation. - (reply: 1)
  273. Plasmid recovery problem - (reply: 3)
  274. Cloning problem using pET32a vector - (reply: 3)
  275. Electroporated plasmid loses the insert - (reply: 8)
  276. emty vector cloning - (reply: 3)
  277. Plasmid sequencing without primers - (reply: 1)
  278. RE site on complimentary strand of plasmid? - (reply: 1)
  279. How much of plasmid should be cut to see the gene - (reply: 1)
  280. Plasmid sequencing - (reply: 1)
  281. Plasmid isolation Qiagen - (reply: 2)
  282. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
  283. Is the T7 tag sequence immunogenic (from pET plasmid) - (reply: 2)
  284. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
  285. Help in RE digestion of vector - (reply: 5)
  286. Amplify fragment in plasmid? - (reply: 4)
  287. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
  288. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  289. Gateway cloning vector compatibility - (reply: 2)
  290. gene expression in E coli with PET21a vector - (reply: 1)
  291. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
  292. Template DNA (plasmid) concentration for PCR - (reply: 4)
  293. co-expression of 2 plasmids in E.coli - (reply: 3)
  294. How to choose the right plasmids for certain types of cell? - (reply: 1)
  295. Plasmid digestion create a bigger plasmid - (reply: 1)
  296. Cloning two fragments into a vector - (reply: 3)
  297. confirmation of insert in to vector - (reply: 3)
  298. whole plasmid pcr? - (reply: 6)
  299. Looking for a GFP plasmid (non-amp) - (reply: 3)
  300. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
  301. ligation of pcr product into expression vector - (reply: 4)
  302. amp plates containing xgal/iptg with expression vector - (reply: 6)
  303. Strange Vector Problems - (reply: 3)
  304. Insert lose in Phage display library - (reply: 6)
  305. extract plasmids from transfected eukaryotic cells - (reply: 6)
  306. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
  307. Lenti vector ligation - (reply: 2)
  308. Cloning from a pGEMT Easy vector - (reply: 3)
  309. Plasmid compatible with pBAD24 - (reply: 1)
  310. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
  311. COL E1 mutations in vector after sequencing - (reply: 1)
  312. Vector with inducible/repressible system - (reply: 1)
  313. Improving expression of recomb proteins in baculovirus - (reply: 1)
  314. Cloning genes into plant vector - (reply: 1)
  315. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
  316. Difference between plasmid DNA extraction and genomic DNA extraction - (reply: 3)
  317. Recombinant construct looks smaller than empty vector - (reply: 3)
  318. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
  319. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
  320. protein expression vector.. - (reply: 4)
  321. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
  322. Lentiviral Vector - Lentiviral Vector (reply: 5)
  323. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
  324. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
  325. Best transcription vector - (reply: 1)
  326. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
  327. which is the best plasmid vector for cloning BSP products? - (reply: 2)
  328. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
  329. Cloning into lentiviral vector - (reply: 11)
  330. use of pGEM -tT easy vectors - (reply: 2)
  331. ~200kb Plasmid DNA purification is not working - (reply: 1)
  332. ligating a 6 His tag to plasmid - (reply: 2)
  333. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
  334. Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
  335. expreesing cloned vector - having problems with texpreesing cloned gene in vector. (reply: 6)
  336. Plasmid sequencing failed why? - (reply: 10)
  337. Lactic Acid Bacteria - Challenges in working with LAB culturing, cloning and plasmid (reply: 7)
  338. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  339. SLIM mutagenesis of large plasmids - (reply: 8)
  340. Introduction to vector development - Basics on vectors types and construction (reply: 1)
  341. Can you receommend a vector? - (reply: 1)
  342. GFP vector as a transfectino efficiency indicator? - (reply: 3)
  343. why 12 h culture for plasmid prep? - (reply: 2)
  344. Big plasmids and Transfection - (reply: 3)
  345. How to add a T7 terminator to a vector? - (reply: 4)
  346. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
  347. Protein expression vectors Please help - (reply: 2)
  348. vectors please help - vectors cleavage sites (reply: 1)
  349. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
  350. very small insert and a very ling vector - (reply: 1)
  351. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
  352. help: why plasmid yield dropped ever since the first culture - (reply: 2)
  353. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
  354. pcr amplify an insert in plasmid? - (reply: 2)
  355. Single-digested vector shifts downward on gel - (reply: 1)
  356. odd plasmids - (reply: 2)
  357. Question about transduction of lentivirus vector into fibroblast ? - transduction (reply: 1)
  358. Lactobacillus plasmid - (reply: 1)
  359. Will transfected plasmids induce cytokine production? - (reply: 1)
  360. Question about plasmid midiprep - (reply: 1)
  361. Plasmid Miniprep yield problems - Low rate of yield using a Miniprep Kit (reply: 10)
  362. Mirror Image Phage Display - "special" contamination (reply: 9)
  363. pGL3 vector - (reply: 3)
  364. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
  365. plasmid isolation - sth lights up in the well of agarose gel (reply: 2)
  366. Overexpression plasmid is not expressing protein - (reply: 13)
  367. Secretion cloning vectors - (reply: 1)
  368. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
  369. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
  370. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
  371. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
  372. PCR problem - basic PCR for plasmid amplification (reply: 2)
  373. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
  374. New to Gene cloning - Plasmid vector preparation (reply: 2)
  375. cloning a 34kb insert - please suggest good vectors (reply: 1)
  376. HELP is needed for low expression using pEXP5-CT vector in BL21 (DE3) plyss - low expression after time course study with 1 mM IPTG (reply: 1)
  377. How long can I store cleaved/digested vector? - (reply: 9)
  378. why cut plasmid DNA with Hind111 and EcoR1? - (reply: 1)
  379. Low yields of plasmids from miniprep - (reply: 1)
  380. A gene is not inserted into the Plasmid - (reply: 2)
  381. purification of PCR product for cloning in a vector - (reply: 3)
  382. Phage Contamination of Cultures? - Phage contamination or somethign else? (reply: 3)
  383. Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
  384. Plasmid Question - (reply: 4)
  385. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
  386. Weird migration of double digested plasmid - (reply: 4)
  387. pMX-PURO Vector not growing - (reply: 3)
  388. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  389. Help for co-transfection of proteins and plasmid - (reply: 1)
  390. subcloning into bacterial expression vector - (reply: 1)
  391. Calculating MOI of viral vector - (reply: 1)
  392. cloning into F1 ori of expression vector??? - (reply: 1)
  393. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
  394. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  395. How to store plasmid DNA on filter paper - I want to make a folder of all of them (reply: 3)
  396. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
  397. Separating cut/non-cut vector - (reply: 6)
  398. ligating small inert into a large vector - (reply: 4)
  399. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
  400. Really strange problem with sequencing and plasmid purification! - (reply: 8)
  401. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  402. plasmid DNA versus genomic DNA - concentrations (reply: 1)
  403. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  404. Undigestable/unrestrictable Plasmid? - (reply: 1)
  405. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  406. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  407. metagenomic library consturction - BAC vectors (reply: 1)
  408. Problem in stable expression of target protein using lentiviral vector - (reply: 3)
  409. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
  410. Suitable vector backbone for in vitro translation in Dros.embryos - (reply: 1)
  411. Viruses and plasmid gaining resistance toward drugs can lead to speciatio - (reply: 3)
  412. pGEX vectors and GST expression - (reply: 3)
  413. problems encountered when working on a large plasmid - (reply: 2)
  414. expression vector with built in promoter - (reply: 1)
  415. make a Mega plasmid mutation - (reply: 1)
  416. Anyone make their own PCR cloning vector? - (reply: 5)
  417. Bicistronic vector vs "normal" vector - (reply: 5)
  418. Can't seem to get insert into vector... please help - (reply: 4)
  419. plasmid cloning - i need help.. can anyone answer me (reply: 5)
  420. Plasmid standard problem - (reply: 2)
  421. Do plasmids work in bacteria? - (reply: 1)
  422. CEN sequences on plasmids (yeast) - (reply: 4)
  423. phage DNA - DNA wont migrate on agarose gel (reply: 3)
  424. Plasmid out of BL21 for mutagensis - (reply: 3)
  425. ORI names - name of a plasmid ori (reply: 11)
  426. uncut vector contamination in gel purified band - (reply: 2)
  427. lentivirus - possible to use 2 transfer vectors? (reply: 1)
  428. Change origin of a plasmid - (reply: 4)
  429. Better to sequence from plasmid? - (reply: 5)
  430. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  431. Does a single colony of E.coli contain more than one plasmid - (reply: 7)
  432. pGL3 promoter vector - (reply: 1)
  433. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  434. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
  435. DNA extraction from induced phage - (reply: 1)
  436. How to get rid of phage contamination - (reply: 1)
  437. lambda phage library - (reply: 4)
  438. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
  439. DNA isolation from plant nuclei by plasmid extraction kit - (reply: 1)
  440. problem with pLK01 plasmid ligation - (reply: 2)
  441. Cloning long fragment...got lots of colonies with vectors that have no inserts.. - (reply: 9)
  442. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  443. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
  444. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
  445. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  446. Dephosphorylation of vector - (reply: 8)
  447. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
  448. Can a single colony contains more than 2 types of insert in their plasmids? - (reply: 2)
  449. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
  450. PCR mutagenesis of plasmid - (reply: 5)
  451. Guanadine HCl for plasmid miniprep - (reply: 3)
  452. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
  453. Large vector cloning-recombination problem? - (reply: 1)
  454. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
  455. Not getting plasmid from miniprep - (reply: 7)
  456. Need advice on cloning very small insert into a vector - (reply: 3)
  457. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
  458. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
  459. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
  460. Digesting large amount of plasmid - (reply: 4)
  461. Vectors with special MCS - (reply: 5)
  462. Cloning <100 bp fragments into a vector - (reply: 7)
  463. Cloning <100 bp fragments into a plasmid - (reply: 7)
  464. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
  465. Help! my plasmid size changed after ligation - (reply: 5)
  466. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
  467. Restriction digestion of gDNA for phage library - (reply: 5)
  468. mammalian expression vector - (reply: 2)
  469. Electroporation doesn't like my plasmid??? - (reply: 4)
  470. Problem with ligation - Insert into the "wrong" vector (reply: 11)
  471. Cloning in pCR2.1 plasmid - (reply: 7)
  472. No expresion of transgene - Retroviral vector (reply: 1)
  473. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  474. lambda phage library - (reply: 1)
  475. More colonies fron vector only control? - (reply: 1)
  476. empty vectors more than the insert - (reply: 6)
  477. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
  478. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
  479. phage display - peptide differentiation (reply: 1)
  480. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  481. ligation of an adaptor into a vector - (reply: 4)
  482. Rnase A for plasmid miniprep - cheaper alternative to qiagen? (reply: 4)
  483. vectors and his-tags - (reply: 3)
  484. miRNA 3´UTR target expression vectors - (reply: 6)
  485. Help cloning large insert into large vector - (reply: 7)
  486. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
  487. problem about vector Dephosphorylation - (reply: 2)
  488. Cloning: Randomly recombined Plasmids? - (reply: 6)
  489. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
  490. plasmid + sepharose - how do this work? (reply: 1)
  491. Transformation and ligation problem - Trasnformation of ligation sample shows only vector size.. (reply: 3)
  492. Anyone uses plasmid to make standard curves? how to make adjustment b/w plates - real time (reply: 4)
  493. How to elute plasmid dried on paper? - I just received plasmid samples by mail (reply: 4)
  494. are vacuum manifolds (plasmid prep) interchange with brands? - (reply: 2)
  495. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  496. Plasmid size problems! - (reply: 3)
  497. Left DNA plasmid out for a week - (reply: 3)
  498. Vector maps - (reply: 3)
  499. Transfecting primary cells with plasmid - (reply: 1)
  500. question about plasmid extraction - (reply: 2)
  501. Ligating 2 Linear fragments into a vector at Once - (reply: 4)
  502. problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
  503. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
  504. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
  505. Disappearing plasmid - (reply: 5)
  506. Transferring Plasmid from Aerobic to Anaerobic Bacteria - a thought about the oil eating microbes (OEMs) (reply: 1)
  507. Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
  508. problem in plasmid transfection - (reply: 2)
  509. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
  510. Plasmid DNA mini-prep - no good isolation (reply: 5)
  511. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
  512. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
  513. plasmid extraction - why dilute (reply: 3)
  514. Suicide vector - (reply: 2)
  515. very high molecular weight plasmid - (reply: 6)
  516. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  517. vectors maps - (reply: 2)
  518. How to dissolve phage library dry powder - (reply: 2)
  519. Fake positive colony in lamda RED reconbination, why? - (reply: 2)
  520. Fake positive colony in lamda RED reconbination, why? - (reply: 3)
  521. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
  522. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  523. Concepts behind plasmid prep - (reply: 2)
  524. help with pCMV-myc vector - (reply: 2)
  525. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
  526. PCR off plasmid for screening - (reply: 2)
  527. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
  528. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  529. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  530. Vector frameshift problem - (reply: 3)
  531. Plasmid frameshift problem after clonning - (reply: 2)
  532. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
  533. plasmid extraction kit wash buffer solution - (reply: 3)
  534. plasmid extraction kit solution composition - wash buffer (reply: 2)
  535. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
  536. are plasmids used for lab work methylated - (reply: 1)
  537. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  538. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  539. Isolation of protein crosslinked to plasmid - (reply: 3)
  540. Inseparable insert in gel purification of the vector - (reply: 3)
  541. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  542. knockdown issue...please help... - shRNA pGIPZ vector (reply: 1)
  543. Which Plasmid DNA for standard curve? - (reply: 5)
  544. pRL-TK as primary Vector ? - (reply: 1)
  545. Ligated Vector Age - When is a ligated vector too old to use? (reply: 1)
  546. GFP insert - trying to insert GFP in a vector (reply: 1)
  547. Plasmid problem - From PCR product (reply: 7)
  548. IFU, MOI, lentiviral vectors - (reply: 1)
  549. viral vector - retroviral vectors (reply: 1)
  550. Gateway and Entry Vectors. - (reply: 1)
  551. Checking PCR insert (into pGEMT vector) - (reply: 2)
  552. Transfection - Plasmid Uptake Control - (reply: 2)
  553. info about the little p in pH - i kept my promise phage!!! (reply: 4)
  554. Culture Grow well but No Plasmid - (reply: 2)
  555. software for plasmid drawing - (reply: 12)
  556. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
  557. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
  558. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
  559. plasmid purification kit for transfection - (reply: 2)
  560. best plasmid purification kit for transfection - (reply: 1)
  561. Expression problem using invitrogen pRSET C vector - (reply: 5)
  562. Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
  563. Which pGL4 vector to choose - (reply: 2)
  564. Vector containing N-his tag and TEV - (reply: 1)
  565. LR reaction - Cloning into a expression vector (reply: 1)
  566. Vector map for pMH1 - Can't find it anywhere! (reply: 2)
  567. Checking Plasmid Expression - (reply: 1)
  568. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  569. about pre-miRNA cloned into lentivirus vectors - (reply: 4)
  570. qPCR determination of phage numbers - slope difference for different phage clones (reply: 2)
  571. PCR a plasmid protein - (reply: 2)
  572. plasmid DNA disappear in the minipre - (reply: 2)
  573. Interpreting a Plasmid Profile gel photo - (reply: 3)
  574. AdEasy Vector System: Why PacI digestion? - (reply: 1)
  575. marker swap plasmid - (reply: 4)
  576. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
  577. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
  578. plasmid dna isolation - (reply: 1)
  579. Vector needed - (reply: 1)
  580. having the wrong vector in my clone - (reply: 2)
  581. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  582. siRNA and plasmid cotransfection - (reply: 1)
  583. rna from plasmid - (reply: 1)
  584. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
  585. Problems facing in cloning with pBI121 vector - (reply: 1)
  586. design parameters for multiple siRNA expression in vector - (reply: 4)
  587. Plasmid curing method - (reply: 4)
  588. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  589. Co-transfection of miRNA and vector expressing target sequence - (reply: 2)
  590. religate the vector - need good protocol! - (reply: 4)
  591. plasmid DNA lab question - (reply: 11)
  592. ShRNA vectors for other than mammalian systems - shRNA for S. pombe (reply: 6)
  593. Problem amplifying plasmid - (reply: 1)
  594. Can linear vectors be transformed and can they express resistance genes? - (reply: 4)
  595. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
  596. Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)
  597. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
  598. HELP! Can't double digest pcDNA3.1 vector - (reply: 6)
  599. Help complementing my mutant in trans - help me find a plasmid (reply: 1)
  600. What about the Baculovirus expression system? - (reply: 2)
  601. Vector NTI from Invitrogen - (reply: 4)
  602. role of glucose in plasmid isolation? - (reply: 1)
  603. Plasmids for protein expression in mammalian cells - (reply: 2)
  604. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
  605. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
  606. Amplfy vector - How to amplify vector (reply: 2)
  607. after re-isolation of vector, get single band - (reply: 4)
  608. Cloning big fragment into smaller vector - (reply: 3)
  609. from where can i get the P1 phage - (reply: 1)
  610. Ligation/Transformation problems & 260/230 ratio of vector - (reply: 4)
  611. plasmid, BAC, or cosmid? - (reply: 3)
  612. Ligation of digested plasmid to hairpin oligo - (reply: 5)
  613. Nucleospin Extract II kit also for plasmids? - (reply: 5)
  614. Best plasmid miniprep? - (reply: 13)
  615. Plasmid instability in E.coli BL21? - (reply: 3)
  616. Difficulty cloning vector with multiple Lox sites - (reply: 2)
  617. Working gene-coding plasmid does not produce transcript or protein - (reply: 2)
  618. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
  619. Vector sequence included in (PCR) amplified insert - (reply: 2)
  620. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
  621. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  622. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
  623. Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL - (reply: 1)
  624. pCLBABE puro retriviral vector - (reply: 1)
  625. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
  626. Plasmid DNA sequence and map - PLASMID DNA (reply: 2)
  627. Plasmid linearization by PCR - (reply: 4)
  628. plasmid sequence identification problem - (reply: 2)
  629. Best lentiviral vector - (reply: 2)
  630. Suitable bacterial reporter vectors - (reply: 3)
  631. plasmid construct problem - (reply: 3)
  632. Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
  633. Transformation problem. Please help! - 1.7kb insert to 5.4kb vector; double digested w/ EcoRV & XhoI (reply: 17)
  634. Transient expression vector - (reply: 11)
  635. How to get plasmid maps from publications? - (reply: 5)
  636. pMSP12-GFP map? - How to get plasmid maps. (reply: 1)
  637. Salmonella vector- non-ColE1 - Suggestion for good Salmonella vector? (reply: 7)
  638. A gateway cloning expression vector question - (reply: 1)
  639. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
  640. File format for plasmids in Vector NTI, Clone Manager etc - I'd like a compatible format with no/minimal data loss (reply: 3)
  641. Ligation Failed, too little Vector??? - Ligation trouble shooting (reply: 5)
  642. Low level of expression - in pASK-IBA7 vector (reply: 6)
  643. How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
  644. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  645. Choice of expression vector - how to choose an expression vector (reply: 1)
  646. New Plasmid Draw/Plotter Program (Free) - (reply: 4)
  647. Plasmid DNA isolation - neutralisation of DNA (reply: 5)
  648. Why the fluoresence is dim or dispear in miRNA vector? - miRNA (reply: 3)
  649. Phage removal of E.coli - (reply: 8)
  650. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
  651. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
  652. Luciferase reporter induced by betagal vector?? - (reply: 1)
  653. PCR problem from transformed TOPO TA vector - (reply: 3)
  654. Struggling for months with one little ligation/transformation - please help! - XhoI digested 1,5 kb insert needs to be ligated into 4,8kb vector (reply: 11)
  655. Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
  656. Self-Ligation of digested vector - (reply: 2)
  657. Ligation issue: Does vector size matter? - (reply: 10)
  658. Plasmid sequence - (reply: 1)
  659. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
  660. Promoters and vectors for shRNA? The need for specific vectors and promoters? - (reply: 4)
  661. Mammalian expression vectors with HA, c-myc, FLAG or anyother tag - The lab is running out of funds and need some cheap source. (reply: 3)
  662. What is stringent vector? - Is it related to level of expression? (reply: 2)
  663. expression vector in E. coli - the best expression vector in E. coli (reply: 5)
  664. pBMN-GFP as episomal vector - (reply: 1)
  665. where can i ontain the binary vector pCB301? - (reply: 1)
  666. plasmid isolation - (reply: 4)
  667. Plasmid supercoiling affecting PCR? - (reply: 2)
  668. Miniprep / plasmid question - How long can you get plasmid? (reply: 5)
  669. TOP10F' - Can they be used for regular vector propagation? (reply: 1)
  670. Cloning done....sequence of the insert for the expression vector - (reply: 1)
  671. heterodimer - bicistronic vector? (reply: 2)
  672. looking for a vector with a Neo cassette, HindIII and BamHI restriction sites (i - (reply: 7)
  673. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  674. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
  675. problem with plasmid extraction - (reply: 3)
  676. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
  677. How to transfect two plasmids in a cell - (reply: 10)
  678. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
  679. How to separate large size plasmids ~12kb - (reply: 8)
  680. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  681. Vector NTI users!!!! please read this!!! - free alternative to vector nti (reply: 9)
  682. low plasmid yield from midi preps - (reply: 4)
  683. Plasmid isolation, help with dilution step? - (reply: 7)
  684. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
  685. full digestion of plasmid to get only dNTPs - (reply: 3)
  686. Lenti viral Vector with GFP - (reply: 4)
  687. inducible shRNA vector system - (reply: 7)
  688. size limit of plasmid origins - (reply: 2)
  689. Creating empty vector by self-ligation - (reply: 31)
  690. How to increase the concentration insert or vector or no cut plasmid - How to increase the concentration insert or vector or no cut plasmid (reply: 4)
  691. confusion with different forms of plasmid - (reply: 3)
  692. pQE vector protein expression - (reply: 3)
  693. zebrafish gene regulation can I use mammel expression vector? - (reply: 1)
  694. Plasmid as standard - Plasmid calculation (reply: 6)
  695. Toxic Plasmid?? Toxic Insert?? - (reply: 2)
  696. ligation problem with SalI degested vector and insert - (reply: 6)
  697. Effect of EtBr on plasmid supercoiling - (reply: 5)
  698. Quantifying plasmid DNA - Nanodrop and Qubit (Invitrogen) suck! (reply: 2)
  699. lentivirus vector problem--help help - (reply: 6)
  700. How to know the exact plasmid size? - - supercoiled, nicked, linear (reply: 6)
  701. Digestion of a plasmid - (reply: 1)
  702. no colonies - Cloning using pENTR vector, no colonies (reply: 2)
  703. Please help Cloning issues with smaI and pUNK1 vector - (reply: 2)
  704. Retroviral vector with luciferase - (reply: 1)
  705. Problems cloning Hairpin vector - (reply: 3)
  706. Sequences of generally used plasmids? - Database for sequences available? (reply: 7)
  707. plasmid copy number - method for determination of plasmid copy number (reply: 2)
  708. Purfiying Unmethylated Plasmid - unmethylated plasmid (reply: 3)
  709. Amplifying plasmid - non specific binding of primer - (reply: 3)
  710. Storing plasmid at 4 degrees C - (reply: 5)
  711. Program to draw plasmids - draw plasmids (reply: 7)
  712. Transfer of gene from one vector to another vector - (reply: 2)
  713. Transfection of vectors - (reply: 8)
  714. CIP required for double digestion of plasmid? - (reply: 3)
  715. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
  716. Plasmid replication - Plasmid replication (reply: 13)
  717. Help with cloning a plasmid that contains repeats! - (reply: 2)
  718. Plasmid extraction sans commercial kits - (reply: 3)
  719. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
  720. Myc Tag Vector - (reply: 1)
  721. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  722. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
  723. mutations in plasmids - (reply: 3)
  724. Gel purification of vector DNA - low yield - (reply: 26)
  725. Sequential restriction digestion of plasmid - (reply: 58)
  726. Problem cloning oligo into double digested vector - (reply: 9)
  727. Big insert to put back into the vector - (reply: 17)
  728. plasmid spin column usage - (reply: 5)
  729. deleting lacZ gene from control vector - (reply: 5)
  730. Vector for conditional knockout - (reply: 1)
  731. What is the biggest insert for a viral vector? - (reply: 15)
  732. Stored bacteria lost plasmids? - (reply: 12)
  733. difference between a linear and circular plasmid/DNA for stable transfections - will a circular plasmid stably integrate into the genome? (reply: 4)
  734. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
  735. cloning problems - empty vector (reply: 3)
  736. Selection during Extraction: Plasmid or Genomic DNA? - (reply: 1)
  737. Anyone have some pEGFP-N1 or N3 vectors? - (reply: 2)
  738. PLASMID ISOLATION - (reply: 10)
  739. Ligation very large vector and small insert - It will not work... (reply: 7)
  740. Frustrated with Plasmid Preps - need help! - (reply: 14)
  741. Can't find the sequences of vector pIJ 702 - Help (reply: 1)
  742. In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)