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Vector Related Discussions
  1. GFP tagged plasmid - (reply: 1)
  2. Designing primers with his-tag for pET28a+ vector - (reply: 2)
  3. a half less of plasmid copy number in the solution resulting from ddPCR than qPC - (reply: 1)
  4. Plasmid purification for Gibson Assembly - (reply: 2)
  5. Co-transfection of multiple plasmids - (reply: 1)
  6. pKD1 based vector for K. lactis expression - (reply: 2)
  7. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
  8. what‘s phage display principle - (reply: 1)
  9. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
  10. pET32b+ vector issue - (reply: 1)
  11. Are relaxed plasmids origin of rep sensitive to bp substitution in RNA I/II sequ - (reply: 5)
  12. How stable is double digested plasmid - (reply: 7)
  13. Mature miRNA in expression vector? - (reply: 1)
  14. Ligation: insert & vector are about the same size - (reply: 3)
  15. low plasmid concentration - (reply: 1)
  16. What make a plasmid candidate for stable transfection - (reply: 1)
  17. Lower Plasmid Yields - (reply: 5)
  18. Cloning - Miniprep plasmid contaminated by gDNA? - (reply: 1)
  19. Question on plasmids - (reply: 4)
  20. *NO* DNA eluted from plasmid prep - Why? - (reply: 12)
  21. pUC and pSC101 vectors in the same cell - (reply: 3)
  22. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
  23. Antisense vectors confused about construction - (reply: 1)
  24. Double Digest pCD Vector - (reply: 3)
  25. Double digestion problem with PUCsp vector - (reply: 1)
  26. How much plasmid for transformation? - (reply: 8)
  27. replicating a mammalian expression vector in bacteria - (reply: 3)
  28. preparing a vector for transfection - (reply: 2)
  29. HELP: From Plasmid Vector to Bacterial Chromosome - (reply: 2)
  30. Suitable ratio of vector and insert in cloning - (reply: 5)
  31. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
  32. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
  33. plasmid repositories - (reply: 2)
  34. How to insert PreScission protease into a vector - (reply: 1)
  35. Why the insert become shorter after inserting into plasmid? - (reply: 2)
  36. real time qPCR standard range plasmid - (reply: 3)
  37. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
  38. Cloning a big insert in a small vector - (reply: 2)
  39. How to know the plasmid extracted are indeed the plasmid of interest? - (reply: 4)
  40. Plasmids recombining - (reply: 3)
  41. Plasmid Methylation - (reply: 3)
  42. Why are there two origin of replications in plasmids - (reply: 3)
  43. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
  44. Plasmid Amplification Issue - (reply: 6)
  45. ratios insert/vector - (reply: 3)
  46. Swapping promoter of destination vector not working. Please help! - (reply: 2)
  47. IRES in expression vector - (reply: 1)
  48. Why do we create own customised plasmid rather than buying iy? - (reply: 1)
  49. Can't isolate plasmid from cloned E.coli - (reply: 4)
  50. Denatured plasmid for pcr - (reply: 1)
  51. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
  52. Problems with M13 phage - (reply: 5)
  53. Screen for F plasmid in e. coli - (reply: 1)
  54. Plasmid Linearization Problems - (reply: 9)
  55. Changing the vector - (reply: 55)
  56. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
  57. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
  58. plasmid construction - (reply: 2)
  59. vector propagation in E coli - (reply: 1)
  60. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  61. Relationship between plasmid size and amount of lipofectamine needed? - (reply: 1)
  62. Can the pcDNA5/FRT/TO vector be used for transient overexpression? - (reply: 2)
  63. direction of the expression units in multi-gene vectors - (reply: 1)
  64. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
  65. ethanol precipitation plasmid DNA - (reply: 3)
  66. Plasmid storage conditions - (reply: 5)
  67. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
  68. Temperature sensitivity of Plasmids - (reply: 1)
  69. New (free) plasmid mapping program - (reply: 4)
  70. filling the digested vector and insert for blunt end cloning - (reply: 2)
  71. removing part from plasmid - (reply: 18)
  72. Help with plasmid extraction and redissolving? - (reply: 5)
  73. Nanodrop machine error message when measuring plasmid DNA concentration - (reply: 3)
  74. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  75. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
  76. Promoter sequence search in a plasmid map - (reply: 3)
  77. Heat vs pH during plasmid extraction - (reply: 8)
  78. Sterility issues with plasmid reconstitution buffer? - (reply: 3)
  79. Heating elution buffer for plasmid prep - (reply: 1)
  80. Cloning into pVIVO2 vector - (reply: 8)
  81. Cloning worked, but the expression vector is weird - (reply: 6)
  82. Using pBABE plasmids for regular transfection - (reply: 1)
  83. Rxn not working - amplify/linearize plasmid - (reply: 3)
  84. Cloning two genes in the same plasmid with one promotor - (reply: 4)
  85. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
  86. RNA contamination in plasmid extracted - (reply: 1)
  87. Problem in plasmid digestion after transformation - (reply: 4)
  88. Call for help! Plasmid extraction problem - (reply: 4)
  89. E. coli growing in LB-ampicillin medium without plasmid? Or miniprep problem? - (reply: 2)
  90. How can addgene sell plasmids with patented technology - (reply: 2)
  91. Transient transfection with large plasmid - (reply: 3)
  92. How to obtain Vectors used in articles - (reply: 5)
  93. Is it generally acceptable to ask another lab for cell line/plasmid/etc - (reply: 4)
  94. Gateway vectors - (reply: 3)
  95. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
  96. Plasmids DNA on filter paper - (reply: 1)
  97. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
  98. Kozak sequence and mammalian expression vector - (reply: 3)
  99. GFP vector - (reply: 1)
  100. Problem with Large vector & very small insert - (reply: 1)
  101. Storage life of plasmid DNA in water - (reply: 5)
  102. Insert & vector too dilute to perform ligation - (reply: 3)
  103. Plasmid DNA digestion problem - (reply: 3)
  104. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
  105. free online DNA & plasmid editing tool - (reply: 1)
  106. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
  107. Appearance of a salty plasmid preparation - (reply: 1)
  108. Common RE site on complementary strands in a Vector - (reply: 7)
  109. Transformation of Restricted plasmid - (reply: 3)
  110. Why is my insert missing from my plasmid? - (reply: 4)
  111. Plasmid digestion and gel purification using HindIII - (reply: 3)
  112. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
  113. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
  114. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  115. Colonies grown without plasmid - (reply: 7)
  116. Reconstituting plasmid DNA from blotting paper - water vs. TE buffer - (reply: 6)
  117. Plasmid on blotting paper - (reply: 1)
  118. Is it possible that the undigested version of this plasmid will be above the lin - (reply: 1)
  119. vector dephosphorylation - (reply: 3)
  120. What plasmid sizes do you work with for SDM? - (reply: 1)
  121. Running Plasmid DNA on Agarose Gel (Banding Pattern) - (reply: 1)
  122. Adshuttle vectors and adenoviruses - (reply: 1)
  123. Plasmid Purification Using an Alkaline Lysis - (reply: 1)
  124. cloning help; high salt level in vector - (reply: 4)
  125. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
  126. How to get rid of bacterial phage from a caterpillar surface? - (reply: 7)
  127. Running undigested plasmids on gel - (reply: 2)
  128. Plasmid isolation from Enterococcus - (reply: 1)
  129. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
  130. Software for vector sequence manipulation - (reply: 2)
  131. overexpress mature microRNA by siRNA vector - (reply: 2)
  132. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
  133. Whole plasmid amplification by PCR - (reply: 2)
  134. For promoter assay, which vector I should use? - (reply: 5)
  135. Sequencing of the cloned plasmid - (reply: 5)
  136. 4 way cloning into TA Vector - (reply: 10)
  137. Plasmid DNA contamination - (reply: 5)
  138. Problems with plasmid DNA extraction - (reply: 3)
  139. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
  140. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
  141. Can I quantify restricted plasmid and oligos? - (reply: 3)
  142. conditionally active plasmids - (reply: 1)
  143. Transient transfection of linearized plasmids - (reply: 3)
  144. Transfecting three plasmids into hES cells - (reply: 2)
  145. HUVEC plasmid transfection challenges - (reply: 2)
  146. Plasmid DNA - (reply: 3)
  147. Electroporate lentiviral vector for stable cell line - (reply: 2)
  148. Genomic DNA and plasmid DNA isolation - (reply: 3)
  149. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
  150. faint band after plasmid extraction - (reply: 7)
  151. pTRIPZ lentiviral vector induction - (reply: 1)
  152. pGEX-KG Vector insert in BL21 Codon Plus RP, Growth Difficulty - (reply: 4)
  153. plasmid extraction troubleshooting,please help !! - (reply: 9)
  154. plasmid digestion - problem with enzymes? - (reply: 4)
  155. Plasmid stability in Ecoli during storage - (reply: 3)
  156. which to use: miR or miRexpression vector - (reply: 1)
  157. Ligation with small insert and large vector - (reply: 6)
  158. Lost of Tet-on Vector - (reply: 3)
  159. Lentiviral 3rd gen transfer vector used with a 2nd generation packaging system - (reply: 1)
  160. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
  161. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
  162. Determining in frame in modified vector - (reply: 1)
  163. Creating primers to add restriction sites to vector backbone - (reply: 7)
  164. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
  165. Low copy vector for library generation - (reply: 2)
  166. Plasmid isolation from gram positive bacteria - (reply: 2)
  167. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
  168. problems with plasmid miniprep - (reply: 8)
  169. shRNA interference using Lenti virus vector - (reply: 1)
  170. Determining the amount of plasmid transfected - (reply: 1)
  171. How to check for the presence of plasmid? - (reply: 3)
  172. PCR with Plasmid recovered from filter paper - (reply: 6)
  173. Check contamination - how to sterilize vector - (reply: 1)
  174. Help for pGEM-T easy vector ligation problem - (reply: 2)
  175. pkp 59 vector - (reply: 2)
  176. Vector insert ratio in cloning - (reply: 3)
  177. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
  178. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
  179. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  180. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
  181. restricted PCR plasmid runs slower - (reply: 2)
  182. High frequency of vector religation - (reply: 10)
  183. Plasmid compatibly groups - (reply: 5)
  184. Plasmid isolation from agrobacterium - (reply: 1)
  185. Cutting my plasmid made it larger - (reply: 7)
  186. What are the "mammalian vectors N1 and C1"? - (reply: 1)
  187. Packaging vectors - (reply: 1)
  188. Recreate original plasmid by cutting out insert - (reply: 2)
  189. posting sequences of plasmid? - (reply: 3)
  190. Incomplete digestion of plasmid with single enzyme - (reply: 1)
  191. problem with plasmid digestion - (reply: 5)
  192. Protein Expression vector with small tag - (reply: 1)
  193. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
  194. unexpected band in PCR with plasmid - (reply: 4)
  195. Sequencing Primers and Plasmids - (reply: 3)
  196. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
  197. Replication of the Plasmid DNA - (reply: 6)
  198. expression plasmids for tRNA - (reply: 6)
  199. cloning pcr insert into plasmid - (reply: 2)
  200. Re-culturing empty plasmid - (reply: 1)
  201. microRNA promoter based expression vector? - (reply: 1)
  202. Can anyone recommend a good stable expression vector for human colorectal cancer - (reply: 1)
  203. help with a vector - (reply: 3)
  204. Adding T7 promoter sequence to vector - (reply: 2)
  205. Plasmid Miniprep - (reply: 2)
  206. Lipofectamin 2000 and Large Plasmid Transfection Optimization - (reply: 3)
  207. Tricistronic vector - (reply: 1)
  208. phage lysate purification - (reply: 2)
  209. Plasmid DNA at RT for a few days - (reply: 4)
  210. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
  211. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
  212. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
  213. Lentviral vectors - (reply: 1)
  214. plasmid rearrangement or sabotage - (reply: 2)
  215. pLDR20 Vector - (reply: 1)
  216. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  217. Linearising plasmid before transfection? - (reply: 2)
  218. Smear in plasmid sample - (reply: 9)
  219. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
  220. vector dephosphorylation - (reply: 5)
  221. Where can I purchase T2 or T4 phage genomic DNA? - (reply: 2)
  222. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
  223. cloning a gene in to a plasmid - (reply: 5)
  224. Characterizing an unknown plasmid - (reply: 6)
  225. Plasmid Purification and Digestion Problem - (reply: 2)
  226. PDCD4 Plasmid, RAW 264.7 not working Help - (reply: 2)
  227. QuikChange with Different plasmids - (reply: 1)
  228. Co-transfection with 2 plasmids - (reply: 10)
  229. Vector Digestion Problem - (reply: 4)
  230. Single Plasmid Gel Band - (reply: 1)
  231. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
  232. Transformation with a big plasmid - (reply: 4)
  233. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  234. Best ratio of 5 Plasmids for Lentivirus production - (reply: 4)
  235. digested plasmid - (reply: 2)
  236. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
  237. isolation of plasmid (midi and maxi prep) - (reply: 18)
  238. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
  239. How to clone insert with one sticky and one blunt end into vector? - (reply: 1)
  240. pUB Vector with two promoters - (reply: 6)
  241. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
  242. pkD46 vector growth in E.coli problem - (reply: 8)
  243. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
  244. Clean genomic DNA and plasmid DNA extraction from E.coli - (reply: 3)
  245. plasmid tetR and tet promoter - (reply: 2)
  246. Trouble in phage display - (reply: 1)
  247. Plasmid clone in BSP - (reply: 1)
  248. Swich selection in plasmid - (reply: 3)
  249. A good vector to make gene library? - (reply: 5)
  250. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
  251. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
  252. Hypothetical Synthetic Plasmid - (reply: 9)
  253. How many fragments in plasmid DNA prep? - (reply: 1)
  254. Problem with plasmids transformation - (reply: 6)
  255. Calcium chloride plasmid transfection - (reply: 1)
  256. How Much Plasmid? - (reply: 6)
  257. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
  258. Plasmid isolation - (reply: 1)
  259. plasmid;GI; ACESSION NUMBER - (reply: 2)
  260. virus transduction vs plasmid transfection - (reply: 4)
  261. How dangerous are non-viral vectors? - (reply: 3)
  262. having trouble getting insert ligation into vector - (reply: 3)
  263. Bizarre changes in plasmid band size - (reply: 3)
  264. where to get vector overexpressing caveolin-1? - (reply: 2)
  265. plasmid repository - (reply: 2)
  266. LZRS vector details - (reply: 1)
  267. transient transfection of retroviral vector without retroviral particle porudcti - (reply: 1)
  268. 12.4kb vector 6.7kb insert - (reply: 2)
  269. pGEM-T Easy vector contamination - (reply: 2)
  270. How to transfer His-tag from N- to C-terminus in plasmid - (reply: 1)
  271. High molecular plasmid isolation form kit and alkaline lysis - (reply: 2)
  272. Help: transduction of primary blood cells (T): lentiviral vs. adenoviral vector - (reply: 1)
  273. I need bynary vector pART27 - (reply: 1)
  274. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
  275. pCCL vector map - (reply: 2)
  276. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
  277. Pls help me with this plasmid!!! - (reply: 1)
  278. Standard curve using plasmid - (reply: 1)
  279. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  280. Methods of plasmid transformation? only heat shock? - (reply: 1)
  281. Cloning into inducible vector but no expression - (reply: 1)
  282. e coli vector and host selection - (reply: 1)
  283. No plasmid after a maxi prep mystery - (reply: 4)
  284. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
  285. Co-expression of 2 plasmids in E.coli - (reply: 1)
  286. How to avoid plasmid instability - (reply: 4)
  287. Plasmid vs cDNA amplification by PCR - (reply: 2)
  288. Shear-induced damage of plasmid by vortexing - (reply: 1)
  289. RNase Band in old plasmid stored at -40 - (reply: 2)
  290. Plasmid vector for yeast and E. coli - (reply: 2)
  291. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
  292. pGEX vector - (reply: 2)
  293. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  294. Filamentous phage infection - (reply: 2)
  295. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  296. how to find suitable vectors to transform wild-type bacteria - (reply: 10)
  297. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
  298. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  299. Plasmid digestion after transformation - (reply: 4)
  300. Blunt end ligation insert:vector ratio - (reply: 1)
  301. Smear above plasmid dna - (reply: 3)
  302. result of plasmid extraction - (reply: 2)
  303. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
  304. plasmid extraction - (reply: 5)
  305. agarose gel electrophoresis of plasmid extraction - (reply: 12)
  306. plasmid extraction - (reply: 3)
  307. Buy BAC vectors - (reply: 1)
  308. Is that a Plasmid??? - (reply: 5)
  309. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
  310. ask for a strain for the suicide vector called E. coli S17-1 pir - (reply: 1)
  311. plasmid extraction - (reply: 13)
  312. ligation colonies contain empty vector.. - (reply: 9)
  313. Is it posssible using shRNA vector to generate microRNA? - (reply: 2)
  314. Cloning two genes in the same plasmid - (reply: 4)
  315. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
  316. Best plasmid for GFP + Mammalian Selection - (reply: 1)
  317. Plasmid, gene orientation, insert orientation. - (reply: 1)
  318. Plasmid recovery problem - (reply: 3)
  319. Cloning problem using pET32a vector - (reply: 3)
  320. Electroporated plasmid loses the insert - (reply: 8)
  321. emty vector cloning - (reply: 3)
  322. Plasmid sequencing without primers - (reply: 1)
  323. RE site on complimentary strand of plasmid? - (reply: 1)
  324. How much of plasmid should be cut to see the gene - (reply: 1)
  325. Plasmid sequencing - (reply: 1)
  326. Plasmid isolation Qiagen - (reply: 2)
  327. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
  328. Is the T7 tag sequence immunogenic (from pET plasmid) - (reply: 2)
  329. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
  330. Help in RE digestion of vector - (reply: 5)
  331. Amplify fragment in plasmid? - (reply: 4)
  332. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
  333. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  334. Gateway cloning vector compatibility - (reply: 2)
  335. gene expression in E coli with PET21a vector - (reply: 1)
  336. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
  337. Template DNA (plasmid) concentration for PCR - (reply: 4)
  338. co-expression of 2 plasmids in E.coli - (reply: 3)
  339. How to choose the right plasmids for certain types of cell? - (reply: 1)
  340. Plasmid digestion create a bigger plasmid - (reply: 1)
  341. Cloning two fragments into a vector - (reply: 3)
  342. confirmation of insert in to vector - (reply: 3)
  343. whole plasmid pcr? - (reply: 6)
  344. Looking for a GFP plasmid (non-amp) - (reply: 3)
  345. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
  346. ligation of pcr product into expression vector - (reply: 4)
  347. amp plates containing xgal/iptg with expression vector - (reply: 6)
  348. Strange Vector Problems - (reply: 3)
  349. Insert lose in Phage display library - (reply: 6)
  350. extract plasmids from transfected eukaryotic cells - (reply: 6)
  351. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
  352. Lenti vector ligation - (reply: 2)
  353. Cloning from a pGEMT Easy vector - (reply: 3)
  354. Plasmid compatible with pBAD24 - (reply: 1)
  355. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
  356. COL E1 mutations in vector after sequencing - (reply: 1)
  357. Vector with inducible/repressible system - (reply: 1)
  358. Improving expression of recomb proteins in baculovirus - (reply: 1)
  359. Cloning genes into plant vector - (reply: 1)
  360. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
  361. Difference between plasmid DNA extraction and genomic DNA extraction - (reply: 3)
  362. Recombinant construct looks smaller than empty vector - (reply: 3)
  363. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
  364. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
  365. protein expression vector.. - (reply: 4)
  366. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
  367. Lentiviral Vector - Lentiviral Vector (reply: 5)
  368. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
  369. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
  370. Best transcription vector - (reply: 1)
  371. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
  372. which is the best plasmid vector for cloning BSP products? - (reply: 2)
  373. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
  374. Cloning into lentiviral vector - (reply: 11)
  375. use of pGEM -tT easy vectors - (reply: 2)
  376. ~200kb Plasmid DNA purification is not working - (reply: 1)
  377. ligating a 6 His tag to plasmid - (reply: 2)
  378. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
  379. Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
  380. expreesing cloned vector - having problems with texpreesing cloned gene in vector. (reply: 6)
  381. Plasmid sequencing failed why? - (reply: 10)
  382. Lactic Acid Bacteria - Challenges in working with LAB culturing, cloning and plasmid (reply: 7)
  383. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  384. SLIM mutagenesis of large plasmids - (reply: 8)
  385. Introduction to vector development - Basics on vectors types and construction (reply: 1)
  386. Can you receommend a vector? - (reply: 1)
  387. GFP vector as a transfectino efficiency indicator? - (reply: 3)
  388. why 12 h culture for plasmid prep? - (reply: 2)
  389. Big plasmids and Transfection - (reply: 3)
  390. How to add a T7 terminator to a vector? - (reply: 4)
  391. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
  392. Protein expression vectors Please help - (reply: 2)
  393. vectors please help - vectors cleavage sites (reply: 1)
  394. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
  395. very small insert and a very ling vector - (reply: 1)
  396. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
  397. help: why plasmid yield dropped ever since the first culture - (reply: 2)
  398. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
  399. pcr amplify an insert in plasmid? - (reply: 2)
  400. Single-digested vector shifts downward on gel - (reply: 1)
  401. odd plasmids - (reply: 2)
  402. Question about transduction of lentivirus vector into fibroblast ? - transduction (reply: 1)
  403. Lactobacillus plasmid - (reply: 1)
  404. Will transfected plasmids induce cytokine production? - (reply: 1)
  405. Question about plasmid midiprep - (reply: 1)
  406. Plasmid Miniprep yield problems - Low rate of yield using a Miniprep Kit (reply: 10)
  407. Mirror Image Phage Display - "special" contamination (reply: 9)
  408. pGL3 vector - (reply: 3)
  409. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
  410. plasmid isolation - sth lights up in the well of agarose gel (reply: 2)
  411. Overexpression plasmid is not expressing protein - (reply: 13)
  412. Secretion cloning vectors - (reply: 1)
  413. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
  414. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
  415. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
  416. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
  417. PCR problem - basic PCR for plasmid amplification (reply: 2)
  418. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
  419. New to Gene cloning - Plasmid vector preparation (reply: 2)
  420. cloning a 34kb insert - please suggest good vectors (reply: 1)
  421. HELP is needed for low expression using pEXP5-CT vector in BL21 (DE3) plyss - low expression after time course study with 1 mM IPTG (reply: 1)
  422. How long can I store cleaved/digested vector? - (reply: 9)
  423. why cut plasmid DNA with Hind111 and EcoR1? - (reply: 1)
  424. Low yields of plasmids from miniprep - (reply: 1)
  425. A gene is not inserted into the Plasmid - (reply: 2)
  426. purification of PCR product for cloning in a vector - (reply: 3)
  427. Phage Contamination of Cultures? - Phage contamination or somethign else? (reply: 3)
  428. Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
  429. Plasmid Question - (reply: 4)
  430. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
  431. Weird migration of double digested plasmid - (reply: 4)
  432. pMX-PURO Vector not growing - (reply: 3)
  433. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  434. Help for co-transfection of proteins and plasmid - (reply: 1)
  435. subcloning into bacterial expression vector - (reply: 1)
  436. Calculating MOI of viral vector - (reply: 1)
  437. cloning into F1 ori of expression vector??? - (reply: 1)
  438. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
  439. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  440. How to store plasmid DNA on filter paper - I want to make a folder of all of them (reply: 3)
  441. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
  442. Separating cut/non-cut vector - (reply: 6)
  443. ligating small inert into a large vector - (reply: 4)
  444. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
  445. Really strange problem with sequencing and plasmid purification! - (reply: 8)
  446. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  447. plasmid DNA versus genomic DNA - concentrations (reply: 1)
  448. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  449. Undigestable/unrestrictable Plasmid? - (reply: 1)
  450. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  451. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  452. metagenomic library consturction - BAC vectors (reply: 1)
  453. Problem in stable expression of target protein using lentiviral vector - (reply: 3)
  454. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
  455. Suitable vector backbone for in vitro translation in Dros.embryos - (reply: 1)
  456. Viruses and plasmid gaining resistance toward drugs can lead to speciatio - (reply: 3)
  457. pGEX vectors and GST expression - (reply: 3)
  458. problems encountered when working on a large plasmid - (reply: 2)
  459. expression vector with built in promoter - (reply: 1)
  460. make a Mega plasmid mutation - (reply: 1)
  461. Anyone make their own PCR cloning vector? - (reply: 5)
  462. Bicistronic vector vs "normal" vector - (reply: 5)
  463. Can't seem to get insert into vector... please help - (reply: 4)
  464. plasmid cloning - i need help.. can anyone answer me (reply: 5)
  465. Plasmid standard problem - (reply: 2)
  466. Do plasmids work in bacteria? - (reply: 1)
  467. CEN sequences on plasmids (yeast) - (reply: 4)
  468. phage DNA - DNA wont migrate on agarose gel (reply: 3)
  469. Plasmid out of BL21 for mutagensis - (reply: 3)
  470. ORI names - name of a plasmid ori (reply: 11)
  471. uncut vector contamination in gel purified band - (reply: 2)
  472. lentivirus - possible to use 2 transfer vectors? (reply: 1)
  473. Change origin of a plasmid - (reply: 4)
  474. Better to sequence from plasmid? - (reply: 5)
  475. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  476. Does a single colony of E.coli contain more than one plasmid - (reply: 7)
  477. pGL3 promoter vector - (reply: 1)
  478. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  479. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
  480. DNA extraction from induced phage - (reply: 1)
  481. How to get rid of phage contamination - (reply: 1)
  482. lambda phage library - (reply: 4)
  483. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
  484. DNA isolation from plant nuclei by plasmid extraction kit - (reply: 1)
  485. problem with pLK01 plasmid ligation - (reply: 2)
  486. Cloning long fragment...got lots of colonies with vectors that have no inserts.. - (reply: 9)
  487. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  488. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
  489. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
  490. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  491. Dephosphorylation of vector - (reply: 8)
  492. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
  493. Can a single colony contains more than 2 types of insert in their plasmids? - (reply: 2)
  494. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
  495. PCR mutagenesis of plasmid - (reply: 5)
  496. Guanadine HCl for plasmid miniprep - (reply: 3)
  497. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
  498. Large vector cloning-recombination problem? - (reply: 1)
  499. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
  500. Not getting plasmid from miniprep - (reply: 7)
  501. Need advice on cloning very small insert into a vector - (reply: 3)
  502. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
  503. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
  504. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
  505. Digesting large amount of plasmid - (reply: 4)
  506. Vectors with special MCS - (reply: 5)
  507. Cloning <100 bp fragments into a vector - (reply: 7)
  508. Cloning <100 bp fragments into a plasmid - (reply: 7)
  509. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
  510. Help! my plasmid size changed after ligation - (reply: 5)
  511. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
  512. Restriction digestion of gDNA for phage library - (reply: 5)
  513. mammalian expression vector - (reply: 2)
  514. Electroporation doesn't like my plasmid??? - (reply: 4)
  515. Problem with ligation - Insert into the "wrong" vector (reply: 11)
  516. Cloning in pCR2.1 plasmid - (reply: 7)
  517. No expresion of transgene - Retroviral vector (reply: 1)
  518. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  519. lambda phage library - (reply: 1)
  520. More colonies fron vector only control? - (reply: 1)
  521. empty vectors more than the insert - (reply: 6)
  522. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
  523. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
  524. phage display - peptide differentiation (reply: 1)
  525. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  526. ligation of an adaptor into a vector - (reply: 4)
  527. Rnase A for plasmid miniprep - cheaper alternative to qiagen? (reply: 4)
  528. vectors and his-tags - (reply: 3)
  529. miRNA 3´UTR target expression vectors - (reply: 6)
  530. Help cloning large insert into large vector - (reply: 7)
  531. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
  532. problem about vector Dephosphorylation - (reply: 2)
  533. Cloning: Randomly recombined Plasmids? - (reply: 6)
  534. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
  535. plasmid + sepharose - how do this work? (reply: 1)
  536. Transformation and ligation problem - Trasnformation of ligation sample shows only vector size.. (reply: 3)
  537. Anyone uses plasmid to make standard curves? how to make adjustment b/w plates - real time (reply: 4)
  538. How to elute plasmid dried on paper? - I just received plasmid samples by mail (reply: 4)
  539. are vacuum manifolds (plasmid prep) interchange with brands? - (reply: 2)
  540. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  541. Plasmid size problems! - (reply: 3)
  542. Left DNA plasmid out for a week - (reply: 3)
  543. Vector maps - (reply: 3)
  544. Transfecting primary cells with plasmid - (reply: 1)
  545. question about plasmid extraction - (reply: 2)
  546. Ligating 2 Linear fragments into a vector at Once - (reply: 4)
  547. problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
  548. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
  549. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
  550. Disappearing plasmid - (reply: 5)
  551. Transferring Plasmid from Aerobic to Anaerobic Bacteria - a thought about the oil eating microbes (OEMs) (reply: 1)
  552. Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
  553. problem in plasmid transfection - (reply: 2)
  554. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
  555. Plasmid DNA mini-prep - no good isolation (reply: 5)
  556. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
  557. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
  558. plasmid extraction - why dilute (reply: 3)
  559. Suicide vector - (reply: 2)
  560. very high molecular weight plasmid - (reply: 6)
  561. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  562. vectors maps - (reply: 2)
  563. How to dissolve phage library dry powder - (reply: 2)
  564. Fake positive colony in lamda RED reconbination, why? - (reply: 2)
  565. Fake positive colony in lamda RED reconbination, why? - (reply: 3)
  566. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
  567. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  568. Concepts behind plasmid prep - (reply: 2)
  569. help with pCMV-myc vector - (reply: 2)
  570. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
  571. PCR off plasmid for screening - (reply: 2)
  572. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
  573. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  574. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  575. Vector frameshift problem - (reply: 3)
  576. Plasmid frameshift problem after clonning - (reply: 2)
  577. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
  578. plasmid extraction kit wash buffer solution - (reply: 3)
  579. plasmid extraction kit solution composition - wash buffer (reply: 2)
  580. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
  581. are plasmids used for lab work methylated - (reply: 1)
  582. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  583. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  584. Isolation of protein crosslinked to plasmid - (reply: 3)
  585. Inseparable insert in gel purification of the vector - (reply: 3)
  586. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  587. knockdown issue...please help... - shRNA pGIPZ vector (reply: 1)
  588. Which Plasmid DNA for standard curve? - (reply: 5)
  589. pRL-TK as primary Vector ? - (reply: 1)
  590. Ligated Vector Age - When is a ligated vector too old to use? (reply: 1)
  591. GFP insert - trying to insert GFP in a vector (reply: 1)
  592. Plasmid problem - From PCR product (reply: 7)
  593. IFU, MOI, lentiviral vectors - (reply: 1)
  594. viral vector - retroviral vectors (reply: 1)
  595. Gateway and Entry Vectors. - (reply: 1)
  596. Checking PCR insert (into pGEMT vector) - (reply: 2)
  597. Transfection - Plasmid Uptake Control - (reply: 2)
  598. info about the little p in pH - i kept my promise phage!!! (reply: 4)
  599. Culture Grow well but No Plasmid - (reply: 2)
  600. software for plasmid drawing - (reply: 13)
  601. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
  602. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
  603. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
  604. plasmid purification kit for transfection - (reply: 2)
  605. best plasmid purification kit for transfection - (reply: 1)
  606. Expression problem using invitrogen pRSET C vector - (reply: 5)
  607. Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
  608. Which pGL4 vector to choose - (reply: 2)
  609. Vector containing N-his tag and TEV - (reply: 1)
  610. LR reaction - Cloning into a expression vector (reply: 1)
  611. Vector map for pMH1 - Can't find it anywhere! (reply: 2)
  612. Checking Plasmid Expression - (reply: 1)
  613. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  614. about pre-miRNA cloned into lentivirus vectors - (reply: 4)
  615. qPCR determination of phage numbers - slope difference for different phage clones (reply: 2)
  616. PCR a plasmid protein - (reply: 2)
  617. plasmid DNA disappear in the minipre - (reply: 2)
  618. Interpreting a Plasmid Profile gel photo - (reply: 3)
  619. AdEasy Vector System: Why PacI digestion? - (reply: 1)
  620. marker swap plasmid - (reply: 4)
  621. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
  622. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
  623. plasmid dna isolation - (reply: 1)
  624. Vector needed - (reply: 1)
  625. having the wrong vector in my clone - (reply: 3)
  626. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  627. siRNA and plasmid cotransfection - (reply: 1)
  628. rna from plasmid - (reply: 1)
  629. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
  630. Problems facing in cloning with pBI121 vector - (reply: 1)
  631. design parameters for multiple siRNA expression in vector - (reply: 4)
  632. Plasmid curing method - (reply: 4)
  633. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  634. Co-transfection of miRNA and vector expressing target sequence - (reply: 2)
  635. religate the vector - need good protocol! - (reply: 4)
  636. plasmid DNA lab question - (reply: 11)
  637. ShRNA vectors for other than mammalian systems - shRNA for S. pombe (reply: 6)
  638. Problem amplifying plasmid - (reply: 1)
  639. Can linear vectors be transformed and can they express resistance genes? - (reply: 4)
  640. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
  641. Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)
  642. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
  643. HELP! Can't double digest pcDNA3.1 vector - (reply: 6)
  644. Help complementing my mutant in trans - help me find a plasmid (reply: 1)
  645. What about the Baculovirus expression system? - (reply: 2)
  646. Vector NTI from Invitrogen - (reply: 4)
  647. role of glucose in plasmid isolation? - (reply: 1)
  648. Plasmids for protein expression in mammalian cells - (reply: 2)
  649. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
  650. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
  651. Amplfy vector - How to amplify vector (reply: 2)
  652. after re-isolation of vector, get single band - (reply: 4)
  653. Cloning big fragment into smaller vector - (reply: 3)
  654. from where can i get the P1 phage - (reply: 1)
  655. Ligation/Transformation problems & 260/230 ratio of vector - (reply: 4)
  656. plasmid, BAC, or cosmid? - (reply: 3)
  657. Ligation of digested plasmid to hairpin oligo - (reply: 5)
  658. Nucleospin Extract II kit also for plasmids? - (reply: 5)
  659. Best plasmid miniprep? - (reply: 13)
  660. Plasmid instability in E.coli BL21? - (reply: 3)
  661. Difficulty cloning vector with multiple Lox sites - (reply: 2)
  662. Working gene-coding plasmid does not produce transcript or protein - (reply: 2)
  663. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
  664. Vector sequence included in (PCR) amplified insert - (reply: 2)
  665. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
  666. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  667. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
  668. Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL - (reply: 1)
  669. pCLBABE puro retriviral vector - (reply: 1)
  670. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
  671. Plasmid DNA sequence and map - PLASMID DNA (reply: 2)
  672. Plasmid linearization by PCR - (reply: 4)
  673. plasmid sequence identification problem - (reply: 2)
  674. Best lentiviral vector - (reply: 2)
  675. Suitable bacterial reporter vectors - (reply: 3)
  676. plasmid construct problem - (reply: 3)
  677. Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
  678. Transformation problem. Please help! - 1.7kb insert to 5.4kb vector; double digested w/ EcoRV & XhoI (reply: 17)
  679. Transient expression vector - (reply: 11)
  680. How to get plasmid maps from publications? - (reply: 5)
  681. pMSP12-GFP map? - How to get plasmid maps. (reply: 1)
  682. Salmonella vector- non-ColE1 - Suggestion for good Salmonella vector? (reply: 7)
  683. A gateway cloning expression vector question - (reply: 1)
  684. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
  685. File format for plasmids in Vector NTI, Clone Manager etc - I'd like a compatible format with no/minimal data loss (reply: 3)
  686. Ligation Failed, too little Vector??? - Ligation trouble shooting (reply: 5)
  687. Low level of expression - in pASK-IBA7 vector (reply: 6)
  688. How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
  689. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  690. Choice of expression vector - how to choose an expression vector (reply: 1)
  691. New Plasmid Draw/Plotter Program (Free) - (reply: 4)
  692. Plasmid DNA isolation - neutralisation of DNA (reply: 5)
  693. Why the fluoresence is dim or dispear in miRNA vector? - miRNA (reply: 3)
  694. Phage removal of E.coli - (reply: 8)
  695. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
  696. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
  697. Luciferase reporter induced by betagal vector?? - (reply: 1)
  698. PCR problem from transformed TOPO TA vector - (reply: 3)
  699. Struggling for months with one little ligation/transformation - please help! - XhoI digested 1,5 kb insert needs to be ligated into 4,8kb vector (reply: 11)
  700. Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
  701. Self-Ligation of digested vector - (reply: 2)
  702. Ligation issue: Does vector size matter? - (reply: 10)
  703. Plasmid sequence - (reply: 1)
  704. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
  705. Promoters and vectors for shRNA? The need for specific vectors and promoters? - (reply: 4)
  706. Mammalian expression vectors with HA, c-myc, FLAG or anyother tag - The lab is running out of funds and need some cheap source. (reply: 3)
  707. What is stringent vector? - Is it related to level of expression? (reply: 2)
  708. expression vector in E. coli - the best expression vector in E. coli (reply: 5)
  709. pBMN-GFP as episomal vector - (reply: 1)
  710. where can i ontain the binary vector pCB301? - (reply: 1)
  711. plasmid isolation - (reply: 4)
  712. Plasmid supercoiling affecting PCR? - (reply: 2)
  713. Miniprep / plasmid question - How long can you get plasmid? (reply: 5)
  714. TOP10F' - Can they be used for regular vector propagation? (reply: 1)
  715. Cloning done....sequence of the insert for the expression vector - (reply: 1)
  716. heterodimer - bicistronic vector? (reply: 2)
  717. looking for a vector with a Neo cassette, HindIII and BamHI restriction sites (i - (reply: 7)
  718. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  719. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
  720. problem with plasmid extraction - (reply: 3)
  721. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
  722. How to transfect two plasmids in a cell - (reply: 10)
  723. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
  724. How to separate large size plasmids ~12kb - (reply: 8)
  725. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  726. Vector NTI users!!!! please read this!!! - free alternative to vector nti (reply: 10)
  727. low plasmid yield from midi preps - (reply: 4)
  728. Plasmid isolation, help with dilution step? - (reply: 7)
  729. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
  730. full digestion of plasmid to get only dNTPs - (reply: 3)
  731. Lenti viral Vector with GFP - (reply: 4)
  732. inducible shRNA vector system - (reply: 7)
  733. size limit of plasmid origins - (reply: 2)
  734. Creating empty vector by self-ligation - (reply: 31)
  735. How to increase the concentration insert or vector or no cut plasmid - How to increase the concentration insert or vector or no cut plasmid (reply: 4)
  736. confusion with different forms of plasmid - (reply: 3)
  737. pQE vector protein expression - (reply: 3)
  738. zebrafish gene regulation can I use mammel expression vector? - (reply: 1)
  739. Plasmid as standard - Plasmid calculation (reply: 6)
  740. Toxic Plasmid?? Toxic Insert?? - (reply: 2)
  741. ligation problem with SalI degested vector and insert - (reply: 6)
  742. Effect of EtBr on plasmid supercoiling - (reply: 5)
  743. Quantifying plasmid DNA - Nanodrop and Qubit (Invitrogen) suck! (reply: 2)
  744. lentivirus vector problem--help help - (reply: 6)
  745. How to know the exact plasmid size? - - supercoiled, nicked, linear (reply: 6)
  746. Digestion of a plasmid - (reply: 1)
  747. no colonies - Cloning using pENTR vector, no colonies (reply: 2)
  748. Please help Cloning issues with smaI and pUNK1 vector - (reply: 2)
  749. Retroviral vector with luciferase - (reply: 1)
  750. Problems cloning Hairpin vector - (reply: 3)
  751. Sequences of generally used plasmids? - Database for sequences available? (reply: 7)
  752. plasmid copy number - method for determination of plasmid copy number (reply: 2)
  753. Purfiying Unmethylated Plasmid - unmethylated plasmid (reply: 3)
  754. Amplifying plasmid - non specific binding of primer - (reply: 3)
  755. Storing plasmid at 4 degrees C - (reply: 5)
  756. Program to draw plasmids - draw plasmids (reply: 9)
  757. Transfer of gene from one vector to another vector - (reply: 2)
  758. Transfection of vectors - (reply: 8)
  759. CIP required for double digestion of plasmid? - (reply: 3)
  760. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
  761. Plasmid replication - Plasmid replication (reply: 13)
  762. Help with cloning a plasmid that contains repeats! - (reply: 2)
  763. Plasmid extraction sans commercial kits - (reply: 3)
  764. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
  765. Myc Tag Vector - (reply: 1)
  766. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  767. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
  768. mutations in plasmids - (reply: 3)
  769. Gel purification of vector DNA - low yield - (reply: 26)
  770. Sequential restriction digestion of plasmid - (reply: 58)
  771. Problem cloning oligo into double digested vector - (reply: 9)
  772. Big insert to put back into the vector - (reply: 17)
  773. plasmid spin column usage - (reply: 5)
  774. deleting lacZ gene from control vector - (reply: 5)
  775. Vector for conditional knockout - (reply: 1)
  776. What is the biggest insert for a viral vector? - (reply: 15)
  777. Stored bacteria lost plasmids? - (reply: 12)
  778. difference between a linear and circular plasmid/DNA for stable transfections - will a circular plasmid stably integrate into the genome? (reply: 4)
  779. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
  780. cloning problems - empty vector (reply: 3)
  781. Selection during Extraction: Plasmid or Genomic DNA? - (reply: 1)
  782. Anyone have some pEGFP-N1 or N3 vectors? - (reply: 2)
  783. PLASMID ISOLATION - (reply: 10)
  784. Ligation very large vector and small insert - It will not work... (reply: 7)
  785. Frustrated with Plasmid Preps - need help! - (reply: 14)
  786. Can't find the sequences of vector pIJ 702 - Help (reply: 1)
  787. In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)