vector Methods, Protocols and Troubleshootings

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Vector Related Discussions

Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
faint band after plasmid extraction - (reply: 7)
pGEX-KG Vector insert in BL21 Codon Plus RP, Growth Difficulty - (reply: 4)
plasmid extraction troubleshooting,please help !! - (reply: 9)
plasmid digestion - problem with enzymes? - (reply: 4)
Plasmid stability in Ecoli during storage - (reply: 3)
which to use: miR or miRexpression vector - (reply: 1)
Ligation with small insert and large vector - (reply: 6)
Lost of Tet-on Vector - (reply: 3)
Lentiviral 3rd gen transfer vector used with a 2nd generation packaging system - (reply: 1)
How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
Determining in frame in modified vector - (reply: 1)
Creating primers to add restriction sites to vector backbone - (reply: 7)
Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 1)
Low copy vector for library generation - (reply: 2)
Plasmid isolation from gram positive bacteria - (reply: 2)
Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
problems with plasmid miniprep - (reply: 8)
shRNA interference using Lenti virus vector - (reply: 1)
Determining the amount of plasmid transfected - (reply: 1)
How to check for the presence of plasmid? - (reply: 3)
PCR with Plasmid recovered from filter paper - (reply: 6)
Check contamination - how to sterilize vector - (reply: 1)
Help for pGEM-T easy vector ligation problem - (reply: 2)
pkp 59 vector - (reply: 2)
Vector insert ratio in cloning - (reply: 3)
Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
qPCR from HEK cells transfected with Plasmid - (reply: 4)
10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
restricted PCR plasmid runs slower - (reply: 2)
High frequency of vector religation - (reply: 10)
Plasmid compatibly groups - (reply: 5)
Plasmid isolation from agrobacterium - (reply: 1)
Cutting my plasmid made it larger - (reply: 7)
What are the "mammalian vectors N1 and C1"? - (reply: 1)
Packaging vectors - (reply: 1)
Recreate original plasmid by cutting out insert - (reply: 2)
posting sequences of plasmid? - (reply: 3)
Incomplete digestion of plasmid with single enzyme - (reply: 1)
problem with plasmid digestion - (reply: 5)
Protein Expression vector with small tag - (reply: 1)
Do you have to purify target vector after double digest from MCS piece - (reply: 1)
unexpected band in PCR with plasmid - (reply: 4)
Sequencing Primers and Plasmids - (reply: 3)
Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
Replication of the Plasmid DNA - (reply: 6)
expression plasmids for tRNA - (reply: 5)
cloning pcr insert into plasmid - (reply: 2)
Re-culturing empty plasmid - (reply: 1)
microRNA promoter based expression vector? - (reply: 1)
Can anyone recommend a good stable expression vector for human colorectal cancer - (reply: 1)
help with a vector - (reply: 3)
Adding T7 promoter sequence to vector - (reply: 2)
Plasmid Miniprep - (reply: 2)
Lipofectamin 2000 and Large Plasmid Transfection Optimization - (reply: 3)
Tricistronic vector - (reply: 1)
phage lysate purification - (reply: 2)
Plasmid DNA at RT for a few days - (reply: 4)
different orientation or direction of genes in expression Plasmid vector - (reply: 4)
pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
plasmid rearrangement or sabotage - (reply: 2)
pLDR20 Vector - (reply: 1)
Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
Linearising plasmid before transfection? - (reply: 2)
Smear in plasmid sample - (reply: 9)
Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
vector dephosphorylation - (reply: 5)
Where can I purchase T2 or T4 phage genomic DNA? - (reply: 2)
Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
cloning a gene in to a plasmid - (reply: 5)
Characterizing an unknown plasmid - (reply: 6)
Plasmid Purification and Digestion Problem - (reply: 2)
PDCD4 Plasmid, RAW 264.7 not working Help - (reply: 2)
QuikChange with Different plasmids - (reply: 1)
Co-transfection with 2 plasmids - (reply: 10)
Vector Digestion Problem - (reply: 4)
Single Plasmid Gel Band - (reply: 1)
Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
Transformation with a big plasmid - (reply: 4)
Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
Best ratio of 5 Plasmids for Lentivirus production - (reply: 4)
digested plasmid - (reply: 2)
Plasmid digested with RE used to transform...what will happen? - (reply: 14)
isolation of plasmid (midi and maxi prep) - (reply: 18)
Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
How to clone insert with one sticky and one blunt end into vector? - (reply: 1)
pUB Vector with two promoters - (reply: 6)
Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
pkD46 vector growth in E.coli problem - (reply: 8)
mamallian expression vector that has FLAG tag and GFP - (reply: 3)
Clean genomic DNA and plasmid DNA extraction from E.coli - (reply: 3)
plasmid tetR and tet promoter - (reply: 2)
Plasmid clone in BSP - (reply: 1)
Swich selection in plasmid - (reply: 3)
A good vector to make gene library? - (reply: 5)
Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
Hypothetical Synthetic Plasmid - (reply: 9)
How many fragments in plasmid DNA prep? - (reply: 1)
Problem with plasmids transformation - (reply: 6)
Calcium chloride plasmid transfection - (reply: 1)
How Much Plasmid? - (reply: 3)
can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
Plasmid isolation - (reply: 1)
plasmid；GI; ACESSION NUMBER - (reply: 2)
virus transduction vs plasmid transfection - (reply: 4)
How dangerous are non-viral vectors? - (reply: 3)
having trouble getting insert ligation into vector - (reply: 3)
Bizarre changes in plasmid band size - (reply: 3)
where to get vector overexpressing caveolin-1? - (reply: 2)
plasmid repository - (reply: 2)
LZRS vector details - (reply: 1)
transient transfection of retroviral vector without retroviral particle porudcti - (reply: 1)
12.4kb vector 6.7kb insert - (reply: 2)
pGEM-T Easy vector contamination - (reply: 2)
How to transfer His-tag from N- to C-terminus in plasmid - (reply: 1)
High molecular plasmid isolation form kit and alkaline lysis - (reply: 2)
Help: transduction of primary blood cells (T): lentiviral vs. adenoviral vector - (reply: 1)
I need bynary vector pART27 - (reply: 1)
Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
pCCL vector map - (reply: 2)
Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
Pls help me with this plasmid!!! - (reply: 1)
Standard curve using plasmid - (reply: 1)
PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
Methods of plasmid transformation? only heat shock? - (reply: 1)
Cloning into inducible vector but no expression - (reply: 1)
e coli vector and host selection - (reply: 1)
No plasmid after a maxi prep mystery - (reply: 4)
only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
Co-expression of 2 plasmids in E.coli - (reply: 1)
How to avoid plasmid instability - (reply: 4)
Plasmid vs cDNA amplification by PCR - (reply: 2)
Shear-induced damage of plasmid by vortexing - (reply: 1)
RNase Band in old plasmid stored at -40 - (reply: 2)
Plasmid vector for yeast and E. coli - (reply: 2)
Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
pGEX vector - (reply: 2)
Recommended competent cell for transformation of large plasmids? - (reply: 4)
Filamentous phage infection - (reply: 2)
Primers for Introduction of new restriction sites to a vector - (reply: 3)
how to find suitable vectors to transform wild-type bacteria - (reply: 10)
Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
Plasmid digestion after transformation - (reply: 4)
Blunt end ligation insert:vector ratio - (reply: 1)
Smear above plasmid dna - (reply: 3)
result of plasmid extraction - (reply: 2)
purification of digested vector by agarose eletrophoresis problem - (reply: 4)
plasmid extraction - (reply: 5)
agarose gel electrophoresis of plasmid extraction - (reply: 12)
plasmid extraction - (reply: 3)
Buy BAC vectors - (reply: 1)
Is that a Plasmid??? - (reply: 5)
plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
ask for a strain for the suicide vector called E. coli S17-1 pir - (reply: 1)
plasmid extraction - (reply: 13)
ligation colonies contain empty vector.. - (reply: 9)
Is it posssible using shRNA vector to generate microRNA? - (reply: 2)
Cloning two genes in the same plasmid - (reply: 4)
why my solution II turns cloudy during plasmid extraction? - (reply: 6)
Best plasmid for GFP + Mammalian Selection - (reply: 1)
Plasmid, gene orientation, insert orientation. - (reply: 1)
Plasmid recovery problem - (reply: 3)
Cloning problem using pET32a vector - (reply: 3)
Electroporated plasmid loses the insert - (reply: 8)
emty vector cloning - (reply: 3)
Plasmid sequencing without primers - (reply: 1)
RE site on complimentary strand of plasmid? - (reply: 1)
How much of plasmid should be cut to see the gene - (reply: 1)
Plasmid sequencing - (reply: 1)
Plasmid isolation Qiagen - (reply: 2)
How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
Is the T7 tag sequence immunogenic (from pET plasmid) - (reply: 2)
expression vector for yeast sacchromyces cerevisiae - (reply: 2)
Help in RE digestion of vector - (reply: 5)
Amplify fragment in plasmid? - (reply: 4)
How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
Homemade MiniPreps for Plasmid Purification - (reply: 1)
Gateway cloning vector compatibility - (reply: 2)
gene expression in E coli with PET21a vector - (reply: 1)
plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
Template DNA (plasmid) concentration for PCR - (reply: 4)
co-expression of 2 plasmids in E.coli - (reply: 3)
How to choose the right plasmids for certain types of cell? - (reply: 1)
Plasmid digestion create a bigger plasmid - (reply: 1)
Cloning two fragments into a vector - (reply: 3)
confirmation of insert in to vector - (reply: 3)
whole plasmid pcr? - (reply: 6)
Looking for a GFP plasmid (non-amp) - (reply: 3)
useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
ligation of pcr product into expression vector - (reply: 4)
amp plates containing xgal/iptg with expression vector - (reply: 6)
Strange Vector Problems - (reply: 3)
Insert lose in Phage display library - (reply: 6)
extract plasmids from transfected eukaryotic cells - (reply: 6)
Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
Lenti vector ligation - (reply: 2)
Cloning from a pGEMT Easy vector - (reply: 3)
Plasmid compatible with pBAD24 - (reply: 1)
cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
COL E1 mutations in vector after sequencing - (reply: 1)
Vector with inducible/repressible system - (reply: 1)
Improving expression of recomb proteins in baculovirus - (reply: 1)
Cloning genes into plant vector - (reply: 1)
Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
Difference between plasmid DNA extraction and genomic DNA extraction - (reply: 3)
Recombinant construct looks smaller than empty vector - (reply: 3)
Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
SURE or Stbl competent cells for retroviral vector? - (reply: 2)
protein expression vector.. - (reply: 4)
Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
Lentiviral Vector - Lentiviral Vector (reply: 5)
electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
Best transcription vector - (reply: 1)
Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
which is the best plasmid vector for cloning BSP products? - (reply: 2)
E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
Cloning into lentiviral vector - (reply: 11)
use of pGEM -tT easy vectors - (reply: 2)
~200kb Plasmid DNA purification is not working - (reply: 1)
ligating a 6 His tag to plasmid - (reply: 2)
Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
expreesing cloned vector - having problems with texpreesing cloned gene in vector. (reply: 6)
Plasmid sequencing failed why? - (reply: 10)
Lactic Acid Bacteria - Challenges in working with LAB culturing, cloning and plasmid (reply: 7)
Difference between PCR/cloning DNA in plasmids - (reply: 2)
SLIM mutagenesis of large plasmids - (reply: 8)
Introduction to vector development - Basics on vectors types and construction (reply: 1)
Can you receommend a vector? - (reply: 1)
GFP vector as a transfectino efficiency indicator? - (reply: 3)
why 12 h culture for plasmid prep? - (reply: 2)
Big plasmids and Transfection - (reply: 3)
How to add a T7 terminator to a vector? - (reply: 4)
Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
Protein expression vectors Please help - (reply: 2)
vectors please help - vectors cleavage sites (reply: 1)
Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
very small insert and a very ling vector - (reply: 1)
cDNA for plasmid transformation PLEASE HElP - (reply: 3)
help: why plasmid yield dropped ever since the first culture - (reply: 2)
Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
pcr amplify an insert in plasmid? - (reply: 2)
Single-digested vector shifts downward on gel - (reply: 1)
odd plasmids - (reply: 2)
Question about transduction of lentivirus vector into fibroblast ? - transduction (reply: 1)
Lactobacillus plasmid - (reply: 1)
Will transfected plasmids induce cytokine production? - (reply: 1)
Question about plasmid midiprep - (reply: 1)
Plasmid Miniprep yield problems - Low rate of yield using a Miniprep Kit (reply: 10)
Mirror Image Phage Display - "special" contamination (reply: 9)
pGL3 vector - (reply: 2)
pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
plasmid isolation - sth lights up in the well of agarose gel (reply: 2)
Overexpression plasmid is not expressing protein - (reply: 13)
Secretion cloning vectors - (reply: 1)
dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
PCR problem - basic PCR for plasmid amplification (reply: 2)
outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
New to Gene cloning - Plasmid vector preparation (reply: 2)
cloning a 34kb insert - please suggest good vectors (reply: 1)
HELP is needed for low expression using pEXP5-CT vector in BL21 (DE3) plyss - low expression after time course study with 1 mM IPTG (reply: 1)
How long can I store cleaved/digested vector? - (reply: 9)
why cut plasmid DNA with Hind111 and EcoR1? - (reply: 1)
Low yields of plasmids from miniprep - (reply: 1)
A gene is not inserted into the Plasmid - (reply: 2)
purification of PCR product for cloning in a vector - (reply: 3)
Phage Contamination of Cultures? - Phage contamination or somethign else? (reply: 3)
Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
Plasmid Question - (reply: 4)
uncut plasmid dna vs linearized plasmid gel - (reply: 1)
Weird migration of double digested plasmid - (reply: 4)
pMX-PURO Vector not growing - (reply: 3)
plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
Help for co-transfection of proteins and plasmid - (reply: 1)
subcloning into bacterial expression vector - (reply: 1)
Calculating MOI of viral vector - (reply: 1)
cloning into F1 ori of expression vector??? - (reply: 1)
Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
How to store plasmid DNA on filter paper - I want to make a folder of all of them (reply: 3)
Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
Separating cut/non-cut vector - (reply: 6)
ligating small inert into a large vector - (reply: 4)
Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
Really strange problem with sequencing and plasmid purification! - (reply: 8)
Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
plasmid DNA versus genomic DNA - concentrations (reply: 1)
why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
Undigestable/unrestrictable Plasmid? - (reply: 1)
Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
metagenomic library consturction - BAC vectors (reply: 1)
Problem in stable expression of target protein using lentiviral vector - (reply: 3)
mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
Suitable vector backbone for in vitro translation in Dros.embryos - (reply: 1)
Viruses and plasmid gaining resistance toward drugs can lead to speciatio - (reply: 3)
pGEX vectors and GST expression - (reply: 3)
problems encountered when working on a large plasmid - (reply: 2)
expression vector with built in promoter - (reply: 1)
make a Mega plasmid mutation - (reply: 1)
Anyone make their own PCR cloning vector? - (reply: 5)
Bicistronic vector vs "normal" vector - (reply: 5)
Can't seem to get insert into vector... please help - (reply: 4)
plasmid cloning - i need help.. can anyone answer me (reply: 5)
Plasmid standard problem - (reply: 2)
Do plasmids work in bacteria? - (reply: 1)
CEN sequences on plasmids (yeast) - (reply: 4)
phage DNA - DNA wont migrate on agarose gel (reply: 3)
Plasmid out of BL21 for mutagensis - (reply: 3)
ORI names - name of a plasmid ori (reply: 11)
uncut vector contamination in gel purified band - (reply: 2)
lentivirus - possible to use 2 transfer vectors? (reply: 1)
Change origin of a plasmid - (reply: 4)
Better to sequence from plasmid? - (reply: 5)
About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
Does a single colony of E.coli contain more than one plasmid - (reply: 7)
pGL3 promoter vector - (reply: 1)
A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
DNA extraction from induced phage - (reply: 1)
How to get rid of phage contamination - (reply: 1)
lambda phage library - (reply: 4)
Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
DNA isolation from plant nuclei by plasmid extraction kit - (reply: 1)
problem with pLK01 plasmid ligation - (reply: 2)
Cloning long fragment...got lots of colonies with vectors that have no inserts.. - (reply: 9)
How to delete a domain in a gene? - cloned plasmid (reply: 12)
cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
amount of plasmid for transformation and fusion protein expression - (reply: 1)
Dephosphorylation of vector - (reply: 8)
How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
Can a single colony contains more than 2 types of insert in their plasmids? - (reply: 2)
PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
PCR mutagenesis of plasmid - (reply: 5)
Guanadine HCl for plasmid miniprep - (reply: 3)
Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
Large vector cloning-recombination problem? - (reply: 1)
Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
Not getting plasmid from miniprep - (reply: 7)
Need advice on cloning very small insert into a vector - (reply: 3)
plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
plasmid more than recommended for transfection - will it be toxic? (reply: 1)
Digesting large amount of plasmid - (reply: 4)
Vectors with special MCS - (reply: 5)
Cloning <100 bp fragments into a vector - (reply: 7)
Cloning <100 bp fragments into a plasmid - (reply: 7)
Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
Help! my plasmid size changed after ligation - (reply: 5)
What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
Restriction digestion of gDNA for phage library - (reply: 5)
mammalian expression vector - (reply: 2)
Electroporation doesn't like my plasmid??? - (reply: 4)
Problem with ligation - Insert into the "wrong" vector (reply: 11)
Cloning in pCR2.1 plasmid - (reply: 7)
No expresion of transgene - Retroviral vector (reply: 1)
Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
lambda phage library - (reply: 1)
More colonies fron vector only control? - (reply: 1)
empty vectors more than the insert - (reply: 6)
Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
phage display - peptide differentiation (reply: 1)
Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
ligation of an adaptor into a vector - (reply: 4)
Rnase A for plasmid miniprep - cheaper alternative to qiagen? (reply: 4)
vectors and his-tags - (reply: 3)
miRNA 3´UTR target expression vectors - (reply: 6)
Help cloning large insert into large vector - (reply: 7)
Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
problem about vector Dephosphorylation - (reply: 2)
Cloning: Randomly recombined Plasmids? - (reply: 6)
Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
plasmid + sepharose - how do this work? (reply: 1)
Transformation and ligation problem - Trasnformation of ligation sample shows only vector size.. (reply: 3)
Anyone uses plasmid to make standard curves? how to make adjustment b/w plates - real time (reply: 4)
How to elute plasmid dried on paper? - I just received plasmid samples by mail (reply: 4)
are vacuum manifolds (plasmid prep) interchange with brands? - (reply: 2)
cloned plasmid expression less than original plasmid - after transfection (reply: 4)
Plasmid size problems! - (reply: 3)
Left DNA plasmid out for a week - (reply: 3)
Vector maps - (reply: 3)
Transfecting primary cells with plasmid - (reply: 1)
question about plasmid extraction - (reply: 2)
Ligating 2 Linear fragments into a vector at Once - (reply: 4)
problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
Disappearing plasmid - (reply: 5)
Transferring Plasmid from Aerobic to Anaerobic Bacteria - a thought about the oil eating microbes (OEMs) (reply: 1)
Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
problem in plasmid transfection - (reply: 2)
Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
Plasmid DNA mini-prep - no good isolation (reply: 5)
Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
plasmid extraction - why dilute (reply: 3)
Suicide vector - (reply: 2)
very high molecular weight plasmid - (reply: 6)
purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
vectors maps - (reply: 2)
How to dissolve phage library dry powder - (reply: 2)
Fake positive colony in lamda RED reconbination, why? - (reply: 2)
Fake positive colony in lamda RED reconbination, why? - (reply: 3)
Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
Concepts behind plasmid prep - (reply: 2)
help with pCMV-myc vector - (reply: 2)
Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
PCR off plasmid for screening - (reply: 2)
Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
Vector frameshift problem - (reply: 3)
Plasmid frameshift problem after clonning - (reply: 2)
minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
plasmid extraction kit wash buffer solution - (reply: 3)
plasmid extraction kit solution composition - wash buffer (reply: 2)
Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
are plasmids used for lab work methylated - (reply: 1)
problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
Isolation of protein crosslinked to plasmid - (reply: 3)
Inseparable insert in gel purification of the vector - (reply: 3)
Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
knockdown issue...please help... - shRNA pGIPZ vector (reply: 1)
Which Plasmid DNA for standard curve? - (reply: 5)
pRL-TK as primary Vector ? - (reply: 1)
Ligated Vector Age - When is a ligated vector too old to use? (reply: 1)
GFP insert - trying to insert GFP in a vector (reply: 1)
Plasmid problem - From PCR product (reply: 7)
IFU, MOI, lentiviral vectors - (reply: 1)
viral vector - retroviral vectors (reply: 1)
Gateway and Entry Vectors. - (reply: 1)
Checking PCR insert (into pGEMT vector) - (reply: 2)
Transfection - Plasmid Uptake Control - (reply: 2)
info about the little p in pH - i kept my promise phage!!! (reply: 4)
Culture Grow well but No Plasmid - (reply: 2)
software for plasmid drawing - (reply: 11)
Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
plasmid purification kit for transfection - (reply: 2)
best plasmid purification kit for transfection - (reply: 1)
Expression problem using invitrogen pRSET C vector - (reply: 5)
Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
Which pGL4 vector to choose - (reply: 2)
Vector containing N-his tag and TEV - (reply: 1)
LR reaction - Cloning into a expression vector (reply: 1)
Vector map for pMH1 - Can't find it anywhere! (reply: 2)
Checking Plasmid Expression - (reply: 1)
trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
about pre-miRNA cloned into lentivirus vectors - (reply: 4)
qPCR determination of phage numbers - slope difference for different phage clones (reply: 2)
PCR a plasmid protein - (reply: 2)
plasmid DNA disappear in the minipre - (reply: 2)
Interpreting a Plasmid Profile gel photo - (reply: 3)
AdEasy Vector System: Why PacI digestion? - (reply: 1)
marker swap plasmid - (reply: 4)
Help needed for ligation of large inserts into TOPO vector - (reply: 3)
What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
plasmid dna isolation - (reply: 1)
Vector needed - (reply: 1)
having the wrong vector in my clone - (reply: 2)
Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
siRNA and plasmid cotransfection - (reply: 1)
rna from plasmid - (reply: 1)
cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
Problems facing in cloning with pBI121 vector - (reply: 1)
design parameters for multiple siRNA expression in vector - (reply: 4)
Plasmid curing method - (reply: 4)
Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
Co-transfection of miRNA and vector expressing target sequence - (reply: 2)
religate the vector - need good protocol! - (reply: 4)
plasmid DNA lab question - (reply: 11)
ShRNA vectors for other than mammalian systems - shRNA for S. pombe (reply: 6)
Problem amplifying plasmid - (reply: 1)
Can linear vectors be transformed and can they express resistance genes? - (reply: 4)
Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)
Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
HELP! Can't double digest pcDNA3.1 vector - (reply: 6)
Help complementing my mutant in trans - help me find a plasmid (reply: 1)
What about the Baculovirus expression system? - (reply: 2)
Vector NTI from Invitrogen - (reply: 4)
role of glucose in plasmid isolation? - (reply: 1)
Plasmids for protein expression in mammalian cells - (reply: 2)
One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
Amplfy vector - How to amplify vector (reply: 2)
after re-isolation of vector, get single band - (reply: 4)
Cloning big fragment into smaller vector - (reply: 3)
from where can i get the P1 phage - (reply: 1)
Ligation/Transformation problems & 260/230 ratio of vector - (reply: 4)
plasmid, BAC, or cosmid? - (reply: 3)
Ligation of digested plasmid to hairpin oligo - (reply: 5)
Nucleospin Extract II kit also for plasmids? - (reply: 5)
Best plasmid miniprep? - (reply: 13)
Plasmid instability in E.coli BL21? - (reply: 3)
Difficulty cloning vector with multiple Lox sites - (reply: 2)
Working gene-coding plasmid does not produce transcript or protein - (reply: 2)
to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
Vector sequence included in (PCR) amplified insert - (reply: 2)
Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL - (reply: 1)
pCLBABE puro retriviral vector - (reply: 1)
Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
Plasmid DNA sequence and map - PLASMID DNA (reply: 2)
Plasmid linearization by PCR - (reply: 4)
plasmid sequence identification problem - (reply: 2)
Best lentiviral vector - (reply: 2)
Suitable bacterial reporter vectors - (reply: 3)
plasmid construct problem - (reply: 3)
Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
Transformation problem. Please help! - 1.7kb insert to 5.4kb vector; double digested w/ EcoRV & XhoI (reply: 17)
Transient expression vector - (reply: 11)
How to get plasmid maps from publications? - (reply: 5)
pMSP12-GFP map? - How to get plasmid maps. (reply: 1)
Salmonella vector- non-ColE1 - Suggestion for good Salmonella vector? (reply: 7)
A gateway cloning expression vector question - (reply: 1)
Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
File format for plasmids in Vector NTI, Clone Manager etc - I'd like a compatible format with no/minimal data loss (reply: 3)
Ligation Failed, too little Vector??? - Ligation trouble shooting (reply: 5)
Low level of expression - in pASK-IBA7 vector (reply: 6)
How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
Choice of expression vector - how to choose an expression vector (reply: 1)
New Plasmid Draw/Plotter Program (Free) - (reply: 4)
Plasmid DNA isolation - neutralisation of DNA (reply: 5)
Why the fluoresence is dim or dispear in miRNA vector? - miRNA (reply: 3)
Phage removal of E.coli - (reply: 8)
plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
Luciferase reporter induced by betagal vector?? - (reply: 1)
PCR problem from transformed TOPO TA vector - (reply: 3)
Struggling for months with one little ligation/transformation - please help! - XhoI digested 1,5 kb insert needs to be ligated into 4,8kb vector (reply: 11)
Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
Self-Ligation of digested vector - (reply: 2)
Ligation issue: Does vector size matter? - (reply: 10)
Plasmid sequence - (reply: 1)
How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
Promoters and vectors for shRNA? The need for specific vectors and promoters? - (reply: 4)
Mammalian expression vectors with HA, c-myc, FLAG or anyother tag - The lab is running out of funds and need some cheap source. (reply: 3)
What is stringent vector? - Is it related to level of expression? (reply: 2)
expression vector in E. coli - the best expression vector in E. coli (reply: 5)
pBMN-GFP as episomal vector - (reply: 1)
where can i ontain the binary vector pCB301? - (reply: 1)
plasmid isolation - (reply: 4)
Plasmid supercoiling affecting PCR? - (reply: 2)
Miniprep / plasmid question - How long can you get plasmid? (reply: 5)
TOP10F' - Can they be used for regular vector propagation? (reply: 1)
Cloning done....sequence of the insert for the expression vector - (reply: 1)
heterodimer - bicistronic vector? (reply: 2)
looking for a vector with a Neo cassette, HindIII and BamHI restriction sites (i - (reply: 7)
Low sized bands in plasmid preperations - its not RNA! (reply: 13)
bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
problem with plasmid extraction - (reply: 2)
Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
How to transfect two plasmids in a cell - (reply: 10)
Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
How to separate large size plasmids ~12kb - (reply: 8)
Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
Vector NTI users!!!! please read this!!! - free alternative to vector nti (reply: 9)
low plasmid yield from midi preps - (reply: 4)
Plasmid isolation, help with dilution step? - (reply: 7)
Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
full digestion of plasmid to get only dNTPs - (reply: 3)
Lenti viral Vector with GFP - (reply: 4)
inducible shRNA vector system - (reply: 7)
size limit of plasmid origins - (reply: 2)
Creating empty vector by self-ligation - (reply: 31)
How to increase the concentration insert or vector or no cut plasmid - How to increase the concentration insert or vector or no cut plasmid (reply: 4)
confusion with different forms of plasmid - (reply: 3)
pQE vector protein expression - (reply: 3)
zebrafish gene regulation can I use mammel expression vector? - (reply: 1)
Plasmid as standard - Plasmid calculation (reply: 6)
Toxic Plasmid?? Toxic Insert?? - (reply: 2)
ligation problem with SalI degested vector and insert - (reply: 6)
Effect of EtBr on plasmid supercoiling - (reply: 5)
Quantifying plasmid DNA - Nanodrop and Qubit (Invitrogen) suck! (reply: 2)
lentivirus vector problem--help help - (reply: 6)
How to know the exact plasmid size? - - supercoiled, nicked, linear (reply: 6)
Digestion of a plasmid - (reply: 1)
no colonies - Cloning using pENTR vector, no colonies (reply: 2)
Please help Cloning issues with smaI and pUNK1 vector - (reply: 2)
Retroviral vector with luciferase - (reply: 1)
Problems cloning Hairpin vector - (reply: 3)
Sequences of generally used plasmids? - Database for sequences available? (reply: 7)
plasmid copy number - method for determination of plasmid copy number (reply: 2)
Purfiying Unmethylated Plasmid - unmethylated plasmid (reply: 3)
Amplifying plasmid - non specific binding of primer - (reply: 3)
Storing plasmid at 4 degrees C - (reply: 5)
Program to draw plasmids - draw plasmids (reply: 6)
Transfer of gene from one vector to another vector - (reply: 2)
Transfection of vectors - (reply: 8)
CIP required for double digestion of plasmid? - (reply: 3)
vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
Plasmid replication - Plasmid replication (reply: 13)
Help with cloning a plasmid that contains repeats! - (reply: 2)
Plasmid extraction sans commercial kits - (reply: 3)
Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
Myc Tag Vector - (reply: 1)
Viral Shuttle Plasmid Restriction digests - (reply: 1)
Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
mutations in plasmids - (reply: 3)
Gel purification of vector DNA - low yield - (reply: 26)
Sequential restriction digestion of plasmid - (reply: 58)
Problem cloning oligo into double digested vector - (reply: 9)
Big insert to put back into the vector - (reply: 17)
plasmid spin column usage - (reply: 5)
deleting lacZ gene from control vector - (reply: 5)
Vector for conditional knockout - (reply: 1)
What is the biggest insert for a viral vector? - (reply: 15)
Stored bacteria lost plasmids? - (reply: 12)
difference between a linear and circular plasmid/DNA for stable transfections - will a circular plasmid stably integrate into the genome? (reply: 4)
How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
cloning problems - empty vector (reply: 3)
Selection during Extraction: Plasmid or Genomic DNA? - (reply: 1)
Anyone have some pEGFP-N1 or N3 vectors? - (reply: 2)
PLASMID ISOLATION - (reply: 10)
Ligation very large vector and small insert - It will not work... (reply: 7)
Frustrated with Plasmid Preps - need help! - (reply: 14)
Can't find the sequences of vector pIJ 702 - Help (reply: 1)
In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)