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Vector Related Discussions
  1. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
  2. pCCL vector map - (reply: 2)
  3. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
  4. Pls help me with this plasmid!!! - (reply: 1)
  5. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  6. Methods of plasmid transformation? only heat shock? - (reply: 1)
  7. e coli vector and host selection - (reply: 1)
  8. No plasmid after a maxi prep mystery - (reply: 4)
  9. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
  10. Co-expression of 2 plasmids in E.coli - (reply: 1)
  11. How to avoid plasmid instability - (reply: 4)
  12. Plasmid vs cDNA amplification by PCR - (reply: 2)
  13. Shear-induced damage of plasmid by vortexing - (reply: 1)
  14. RNase Band in old plasmid stored at -40 - (reply: 2)
  15. Plasmid vector for yeast and E. coli - (reply: 2)
  16. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 5)
  17. pGEX vector - (reply: 2)
  18. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  19. Filamentous phage infection - (reply: 2)
  20. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  21. how to find suitable vectors to transform wild-type bacteria - (reply: 10)
  22. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
  23. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  24. Plasmid digestion after transformation - (reply: 3)
  25. Blunt end ligation insert:vector ratio - (reply: 1)
  26. Smear above plasmid dna - (reply: 3)
  27. result of plasmid extraction - (reply: 2)
  28. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
  29. plasmid extraction - (reply: 5)
  30. agarose gel electrophoresis of plasmid extraction - (reply: 12)
  31. plasmid extraction - (reply: 3)
  32. Buy BAC vectors - (reply: 1)
  33. Is that a Plasmid??? - (reply: 5)
  34. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
  35. ask for a strain for the suicide vector called E. coli S17-1 pir - (reply: 1)
  36. plasmid extraction - (reply: 13)
  37. ligation colonies contain empty vector.. - (reply: 9)
  38. Is it posssible using shRNA vector to generate microRNA? - (reply: 2)
  39. Cloning two genes in the same plasmid - (reply: 4)
  40. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
  41. Best plasmid for GFP + Mammalian Selection - (reply: 1)
  42. Plasmid, gene orientation, insert orientation. - (reply: 1)
  43. Plasmid recovery problem - (reply: 3)
  44. Cloning problem using pET32a vector - (reply: 3)
  45. Electroporated plasmid loses the insert - (reply: 8)
  46. emty vector cloning - (reply: 3)
  47. Plasmid sequencing without primers - (reply: 1)
  48. RE site on complimentary strand of plasmid? - (reply: 1)
  49. How much of plasmid should be cut to see the gene - (reply: 1)
  50. Plasmid sequencing - (reply: 1)
  51. Plasmid isolation Qiagen - (reply: 2)
  52. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
  53. Is the T7 tag sequence immunogenic (from pET plasmid) - (reply: 2)
  54. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
  55. Help in RE digestion of vector - (reply: 5)
  56. Amplify fragment in plasmid? - (reply: 4)
  57. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
  58. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  59. Gateway cloning vector compatibility - (reply: 2)
  60. gene expression in E coli with PET21a vector - (reply: 1)
  61. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
  62. Template DNA (plasmid) concentration for PCR - (reply: 4)
  63. co-expression of 2 plasmids in E.coli - (reply: 3)
  64. How to choose the right plasmids for certain types of cell? - (reply: 1)
  65. Plasmid digestion create a bigger plasmid - (reply: 1)
  66. Cloning two fragments into a vector - (reply: 3)
  67. confirmation of insert in to vector - (reply: 3)
  68. whole plasmid pcr? - (reply: 6)
  69. Looking for a GFP plasmid (non-amp) - (reply: 4)
  70. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
  71. ligation of pcr product into expression vector - (reply: 4)
  72. amp plates containing xgal/iptg with expression vector - (reply: 6)
  73. Strange Vector Problems - (reply: 3)
  74. Insert lose in Phage display library - (reply: 6)
  75. extract plasmids from transfected eukaryotic cells - (reply: 6)
  76. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
  77. Lenti vector ligation - (reply: 2)
  78. Cloning from a pGEMT Easy vector - (reply: 3)
  79. Plasmid compatible with pBAD24 - (reply: 1)
  80. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
  81. COL E1 mutations in vector after sequencing - (reply: 1)
  82. Vector with inducible/repressible system - (reply: 1)
  83. Improving expression of recomb proteins in baculovirus - (reply: 1)
  84. Cloning genes into plant vector - (reply: 1)
  85. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
  86. Difference between plasmid DNA extraction and genomic DNA extraction - (reply: 3)
  87. Recombinant construct looks smaller than empty vector - (reply: 3)
  88. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
  89. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
  90. protein expression vector.. - (reply: 4)
  91. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
  92. Lentiviral Vector - Lentiviral Vector (reply: 5)
  93. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
  94. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
  95. Best transcription vector - (reply: 1)
  96. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
  97. which is the best plasmid vector for cloning BSP products? - (reply: 2)
  98. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
  99. Cloning into lentiviral vector - (reply: 10)
  100. use of pGEM -tT easy vectors - (reply: 2)
  101. ~200kb Plasmid DNA purification is not working - (reply: 1)
  102. ligating a 6 His tag to plasmid - (reply: 2)
  103. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
  104. Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
  105. expreesing cloned vector - having problems with texpreesing cloned gene in vector. (reply: 6)
  106. Plasmid sequencing failed why? - (reply: 10)
  107. Lactic Acid Bacteria - Challenges in working with LAB culturing, cloning and plasmid (reply: 7)
  108. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  109. SLIM mutagenesis of large plasmids - (reply: 8)
  110. Introduction to vector development - Basics on vectors types and construction (reply: 1)
  111. Can you receommend a vector? - (reply: 1)
  112. GFP vector as a transfectino efficiency indicator? - (reply: 3)
  113. why 12 h culture for plasmid prep? - (reply: 2)
  114. Big plasmids and Transfection - (reply: 3)
  115. How to add a T7 terminator to a vector? - (reply: 4)
  116. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
  117. Protein expression vectors Please help - (reply: 2)
  118. vectors please help - vectors cleavage sites (reply: 1)
  119. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
  120. very small insert and a very ling vector - (reply: 1)
  121. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
  122. help: why plasmid yield dropped ever since the first culture - (reply: 2)
  123. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
  124. pcr amplify an insert in plasmid? - (reply: 2)
  125. Single-digested vector shifts downward on gel - (reply: 1)
  126. odd plasmids - (reply: 2)
  127. Question about transduction of lentivirus vector into fibroblast ? - transduction (reply: 1)
  128. Lactobacillus plasmid - (reply: 1)
  129. Will transfected plasmids induce cytokine production? - (reply: 1)
  130. Question about plasmid midiprep - (reply: 1)
  131. Plasmid Miniprep yield problems - Low rate of yield using a Miniprep Kit (reply: 10)
  132. Mirror Image Phage Display - "special" contamination (reply: 9)
  133. pGL3 vector - (reply: 2)
  134. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
  135. plasmid isolation - sth lights up in the well of agarose gel (reply: 2)
  136. Overexpression plasmid is not expressing protein - (reply: 11)
  137. Secretion cloning vectors - (reply: 1)
  138. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
  139. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
  140. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
  141. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
  142. PCR problem - basic PCR for plasmid amplification (reply: 2)
  143. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
  144. New to Gene cloning - Plasmid vector preparation (reply: 2)
  145. cloning a 34kb insert - please suggest good vectors (reply: 1)
  146. HELP is needed for low expression using pEXP5-CT vector in BL21 (DE3) plyss - low expression after time course study with 1 mM IPTG (reply: 1)
  147. How long can I store cleaved/digested vector? - (reply: 9)
  148. why cut plasmid DNA with Hind111 and EcoR1? - (reply: 1)
  149. Low yields of plasmids from miniprep - (reply: 1)
  150. A gene is not inserted into the Plasmid - (reply: 2)
  151. purification of PCR product for cloning in a vector - (reply: 3)
  152. Phage Contamination of Cultures? - Phage contamination or somethign else? (reply: 3)
  153. Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
  154. Plasmid Question - (reply: 4)
  155. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
  156. Weird migration of double digested plasmid - (reply: 4)
  157. pMX-PURO Vector not growing - (reply: 3)
  158. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  159. Help for co-transfection of proteins and plasmid - (reply: 1)
  160. subcloning into bacterial expression vector - (reply: 1)
  161. Calculating MOI of viral vector - (reply: 1)
  162. cloning into F1 ori of expression vector??? - (reply: 1)
  163. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
  164. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  165. How to store plasmid DNA on filter paper - I want to make a folder of all of them (reply: 3)
  166. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
  167. Separating cut/non-cut vector - (reply: 6)
  168. ligating small inert into a large vector - (reply: 4)
  169. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
  170. Really strange problem with sequencing and plasmid purification! - (reply: 7)
  171. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  172. plasmid DNA versus genomic DNA - concentrations (reply: 1)
  173. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  174. Undigestable/unrestrictable Plasmid? - (reply: 1)
  175. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  176. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  177. metagenomic library consturction - BAC vectors (reply: 1)
  178. Problem in stable expression of target protein using lentiviral vector - (reply: 3)
  179. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
  180. Suitable vector backbone for in vitro translation in Dros.embryos - (reply: 1)
  181. Viruses and plasmid gaining resistance toward drugs can lead to speciatio - (reply: 3)
  182. pGEX vectors and GST expression - (reply: 3)
  183. problems encountered when working on a large plasmid - (reply: 2)
  184. expression vector with built in promoter - (reply: 1)
  185. make a Mega plasmid mutation - (reply: 1)
  186. Anyone make their own PCR cloning vector? - (reply: 5)
  187. Bicistronic vector vs "normal" vector - (reply: 5)
  188. Can't seem to get insert into vector... please help - (reply: 4)
  189. plasmid cloning - i need help.. can anyone answer me (reply: 5)
  190. Plasmid standard problem - (reply: 2)
  191. Do plasmids work in bacteria? - (reply: 1)
  192. CEN sequences on plasmids (yeast) - (reply: 4)
  193. phage DNA - DNA wont migrate on agarose gel (reply: 3)
  194. Plasmid out of BL21 for mutagensis - (reply: 3)
  195. ORI names - name of a plasmid ori (reply: 11)
  196. uncut vector contamination in gel purified band - (reply: 2)
  197. lentivirus - possible to use 2 transfer vectors? (reply: 1)
  198. Change origin of a plasmid - (reply: 4)
  199. Better to sequence from plasmid? - (reply: 5)
  200. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  201. Does a single colony of E.coli contain more than one plasmid - (reply: 7)
  202. pGL3 promoter vector - (reply: 1)
  203. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  204. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
  205. DNA extraction from induced phage - (reply: 1)
  206. How to get rid of phage contamination - (reply: 1)
  207. lambda phage library - (reply: 4)
  208. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
  209. DNA isolation from plant nuclei by plasmid extraction kit - (reply: 1)
  210. problem with pLK01 plasmid ligation - (reply: 2)
  211. Cloning long fragment...got lots of colonies with vectors that have no inserts.. - (reply: 9)
  212. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  213. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
  214. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
  215. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  216. Dephosphorylation of vector - (reply: 8)
  217. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
  218. Can a single colony contains more than 2 types of insert in their plasmids? - (reply: 2)
  219. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
  220. PCR mutagenesis of plasmid - (reply: 5)
  221. Guanadine HCl for plasmid miniprep - (reply: 3)
  222. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
  223. Large vector cloning-recombination problem? - (reply: 1)
  224. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
  225. Not getting plasmid from miniprep - (reply: 7)
  226. Need advice on cloning very small insert into a vector - (reply: 3)
  227. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
  228. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
  229. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
  230. Digesting large amount of plasmid - (reply: 4)
  231. Vectors with special MCS - (reply: 5)
  232. Cloning <100 bp fragments into a vector - (reply: 7)
  233. Cloning <100 bp fragments into a plasmid - (reply: 7)
  234. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
  235. Help! my plasmid size changed after ligation - (reply: 5)
  236. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
  237. Restriction digestion of gDNA for phage library - (reply: 5)
  238. mammalian expression vector - (reply: 2)
  239. Electroporation doesn't like my plasmid??? - (reply: 4)
  240. Problem with ligation - Insert into the "wrong" vector (reply: 11)
  241. Cloning in pCR2.1 plasmid - (reply: 7)
  242. No expresion of transgene - Retroviral vector (reply: 1)
  243. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  244. lambda phage library - (reply: 1)
  245. More colonies fron vector only control? - (reply: 1)
  246. empty vectors more than the insert - (reply: 6)
  247. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
  248. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
  249. phage display - peptide differentiation (reply: 1)
  250. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  251. ligation of an adaptor into a vector - (reply: 4)
  252. Rnase A for plasmid miniprep - cheaper alternative to qiagen? (reply: 4)
  253. vectors and his-tags - (reply: 3)
  254. miRNA 3´UTR target expression vectors - (reply: 6)
  255. Help cloning large insert into large vector - (reply: 7)
  256. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
  257. problem about vector Dephosphorylation - (reply: 2)
  258. Cloning: Randomly recombined Plasmids? - (reply: 6)
  259. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
  260. plasmid + sepharose - how do this work? (reply: 1)
  261. Transformation and ligation problem - Trasnformation of ligation sample shows only vector size.. (reply: 3)
  262. Anyone uses plasmid to make standard curves? how to make adjustment b/w plates - real time (reply: 4)
  263. How to elute plasmid dried on paper? - I just received plasmid samples by mail (reply: 4)
  264. are vacuum manifolds (plasmid prep) interchange with brands? - (reply: 2)
  265. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  266. Plasmid size problems! - (reply: 3)
  267. Left DNA plasmid out for a week - (reply: 3)
  268. Vector maps - (reply: 2)
  269. Transfecting primary cells with plasmid - (reply: 1)
  270. question about plasmid extraction - (reply: 2)
  271. Ligating 2 Linear fragments into a vector at Once - (reply: 4)
  272. problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
  273. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
  274. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
  275. Disappearing plasmid - (reply: 5)
  276. Transferring Plasmid from Aerobic to Anaerobic Bacteria - a thought about the oil eating microbes (OEMs) (reply: 1)
  277. Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
  278. problem in plasmid transfection - (reply: 2)
  279. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
  280. Plasmid DNA mini-prep - no good isolation (reply: 5)
  281. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
  282. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
  283. plasmid extraction - why dilute (reply: 3)
  284. Suicide vector - (reply: 2)
  285. very high molecular weight plasmid - (reply: 6)
  286. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  287. vectors maps - (reply: 2)
  288. How to dissolve phage library dry powder - (reply: 2)
  289. Fake positive colony in lamda RED reconbination, why? - (reply: 2)
  290. Fake positive colony in lamda RED reconbination, why? - (reply: 3)
  291. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
  292. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  293. Concepts behind plasmid prep - (reply: 2)
  294. help with pCMV-myc vector - (reply: 2)
  295. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
  296. PCR off plasmid for screening - (reply: 2)
  297. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
  298. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  299. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  300. Vector frameshift problem - (reply: 3)
  301. Plasmid frameshift problem after clonning - (reply: 2)
  302. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
  303. plasmid extraction kit wash buffer solution - (reply: 3)
  304. plasmid extraction kit solution composition - wash buffer (reply: 2)
  305. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
  306. are plasmids used for lab work methylated - (reply: 1)
  307. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  308. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  309. Isolation of protein crosslinked to plasmid - (reply: 3)
  310. Inseparable insert in gel purification of the vector - (reply: 3)
  311. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  312. knockdown issue...please help... - shRNA pGIPZ vector (reply: 1)
  313. Which Plasmid DNA for standard curve? - (reply: 5)
  314. pRL-TK as primary Vector ? - (reply: 1)
  315. Ligated Vector Age - When is a ligated vector too old to use? (reply: 1)
  316. GFP insert - trying to insert GFP in a vector (reply: 1)
  317. Plasmid problem - From PCR product (reply: 7)
  318. IFU, MOI, lentiviral vectors - (reply: 1)
  319. viral vector - retroviral vectors (reply: 1)
  320. Gateway and Entry Vectors. - (reply: 1)
  321. Checking PCR insert (into pGEMT vector) - (reply: 2)
  322. Transfection - Plasmid Uptake Control - (reply: 2)
  323. info about the little p in pH - i kept my promise phage!!! (reply: 4)
  324. Culture Grow well but No Plasmid - (reply: 2)
  325. software for plasmid drawing - (reply: 7)
  326. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
  327. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
  328. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
  329. plasmid purification kit for transfection - (reply: 2)
  330. best plasmid purification kit for transfection - (reply: 1)
  331. Expression problem using invitrogen pRSET C vector - (reply: 5)
  332. Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
  333. Which pGL4 vector to choose - (reply: 2)
  334. Vector containing N-his tag and TEV - (reply: 1)
  335. LR reaction - Cloning into a expression vector (reply: 1)
  336. Vector map for pMH1 - Can't find it anywhere! (reply: 2)
  337. Checking Plasmid Expression - (reply: 1)
  338. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  339. about pre-miRNA cloned into lentivirus vectors - (reply: 4)
  340. qPCR determination of phage numbers - slope difference for different phage clones (reply: 2)
  341. PCR a plasmid protein - (reply: 2)
  342. plasmid DNA disappear in the minipre - (reply: 2)
  343. Interpreting a Plasmid Profile gel photo - (reply: 3)
  344. AdEasy Vector System: Why PacI digestion? - (reply: 1)
  345. marker swap plasmid - (reply: 4)
  346. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
  347. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
  348. plasmid dna isolation - (reply: 1)
  349. Vector needed - (reply: 1)
  350. having the wrong vector in my clone - (reply: 2)
  351. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  352. siRNA and plasmid cotransfection - (reply: 1)
  353. rna from plasmid - (reply: 1)
  354. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
  355. Problems facing in cloning with pBI121 vector - (reply: 1)
  356. design parameters for multiple siRNA expression in vector - (reply: 4)
  357. Plasmid curing method - (reply: 4)
  358. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  359. Co-transfection of miRNA and vector expressing target sequence - (reply: 2)
  360. religate the vector - need good protocol! - (reply: 4)
  361. plasmid DNA lab question - (reply: 11)
  362. ShRNA vectors for other than mammalian systems - shRNA for S. pombe (reply: 6)
  363. Problem amplifying plasmid - (reply: 1)
  364. Can linear vectors be transformed and can they express resistance genes? - (reply: 4)
  365. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
  366. Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)
  367. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
  368. HELP! Can't double digest pcDNA3.1 vector - (reply: 6)
  369. Help complementing my mutant in trans - help me find a plasmid (reply: 1)
  370. What about the Baculovirus expression system? - (reply: 2)
  371. Vector NTI from Invitrogen - (reply: 4)
  372. role of glucose in plasmid isolation? - (reply: 1)
  373. Plasmids for protein expression in mammalian cells - (reply: 2)
  374. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
  375. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
  376. Amplfy vector - How to amplify vector (reply: 2)
  377. after re-isolation of vector, get single band - (reply: 4)
  378. Cloning big fragment into smaller vector - (reply: 3)
  379. from where can i get the P1 phage - (reply: 1)
  380. Ligation/Transformation problems & 260/230 ratio of vector - (reply: 4)
  381. plasmid, BAC, or cosmid? - (reply: 3)
  382. Ligation of digested plasmid to hairpin oligo - (reply: 5)
  383. Nucleospin Extract II kit also for plasmids? - (reply: 5)
  384. Best plasmid miniprep? - (reply: 13)
  385. Plasmid instability in E.coli BL21? - (reply: 3)
  386. Difficulty cloning vector with multiple Lox sites - (reply: 2)
  387. Working gene-coding plasmid does not produce transcript or protein - (reply: 2)
  388. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
  389. Vector sequence included in (PCR) amplified insert - (reply: 2)
  390. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
  391. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  392. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
  393. Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL - (reply: 1)
  394. pCLBABE puro retriviral vector - (reply: 1)
  395. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
  396. Plasmid DNA sequence and map - PLASMID DNA (reply: 2)
  397. Plasmid linearization by PCR - (reply: 4)
  398. plasmid sequence identification problem - (reply: 2)
  399. Best lentiviral vector - (reply: 2)
  400. Suitable bacterial reporter vectors - (reply: 3)
  401. plasmid construct problem - (reply: 3)
  402. Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
  403. Transformation problem. Please help! - 1.7kb insert to 5.4kb vector; double digested w/ EcoRV & XhoI (reply: 17)
  404. Transient expression vector - (reply: 11)
  405. How to get plasmid maps from publications? - (reply: 5)
  406. pMSP12-GFP map? - How to get plasmid maps. (reply: 1)
  407. Salmonella vector- non-ColE1 - Suggestion for good Salmonella vector? (reply: 7)
  408. A gateway cloning expression vector question - (reply: 1)
  409. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
  410. File format for plasmids in Vector NTI, Clone Manager etc - I'd like a compatible format with no/minimal data loss (reply: 3)
  411. Ligation Failed, too little Vector??? - Ligation trouble shooting (reply: 5)
  412. Low level of expression - in pASK-IBA7 vector (reply: 6)
  413. How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
  414. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  415. Choice of expression vector - how to choose an expression vector (reply: 1)
  416. New Plasmid Draw/Plotter Program (Free) - (reply: 4)
  417. Plasmid DNA isolation - neutralisation of DNA (reply: 5)
  418. Why the fluoresence is dim or dispear in miRNA vector? - miRNA (reply: 3)
  419. Phage removal of E.coli - (reply: 8)
  420. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
  421. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
  422. Luciferase reporter induced by betagal vector?? - (reply: 1)
  423. PCR problem from transformed TOPO TA vector - (reply: 3)
  424. Struggling for months with one little ligation/transformation - please help! - XhoI digested 1,5 kb insert needs to be ligated into 4,8kb vector (reply: 11)
  425. Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
  426. Self-Ligation of digested vector - (reply: 2)
  427. Ligation issue: Does vector size matter? - (reply: 10)
  428. Plasmid sequence - (reply: 1)
  429. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
  430. Promoters and vectors for shRNA? The need for specific vectors and promoters? - (reply: 4)
  431. Mammalian expression vectors with HA, c-myc, FLAG or anyother tag - The lab is running out of funds and need some cheap source. (reply: 3)
  432. What is stringent vector? - Is it related to level of expression? (reply: 2)
  433. expression vector in E. coli - the best expression vector in E. coli (reply: 5)
  434. pBMN-GFP as episomal vector - (reply: 1)
  435. where can i ontain the binary vector pCB301? - (reply: 1)
  436. plasmid isolation - (reply: 4)
  437. Plasmid supercoiling affecting PCR? - (reply: 2)
  438. Miniprep / plasmid question - How long can you get plasmid? (reply: 5)
  439. TOP10F' - Can they be used for regular vector propagation? (reply: 1)
  440. Cloning done....sequence of the insert for the expression vector - (reply: 1)
  441. heterodimer - bicistronic vector? (reply: 2)
  442. looking for a vector with a Neo cassette, HindIII and BamHI restriction sites (i - (reply: 7)
  443. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  444. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
  445. problem with plasmid extraction - (reply: 2)
  446. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
  447. How to transfect two plasmids in a cell - (reply: 10)
  448. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
  449. How to separate large size plasmids ~12kb - (reply: 8)
  450. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  451. Vector NTI users!!!! please read this!!! - free alternative to vector nti (reply: 9)
  452. low plasmid yield from midi preps - (reply: 4)
  453. Plasmid isolation, help with dilution step? - (reply: 7)
  454. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
  455. full digestion of plasmid to get only dNTPs - (reply: 3)
  456. Lenti viral Vector with GFP - (reply: 4)
  457. inducible shRNA vector system - (reply: 7)
  458. size limit of plasmid origins - (reply: 2)
  459. Creating empty vector by self-ligation - (reply: 31)
  460. How to increase the concentration insert or vector or no cut plasmid - How to increase the concentration insert or vector or no cut plasmid (reply: 4)
  461. confusion with different forms of plasmid - (reply: 3)
  462. pQE vector protein expression - (reply: 3)
  463. zebrafish gene regulation can I use mammel expression vector? - (reply: 1)
  464. Plasmid as standard - Plasmid calculation (reply: 6)
  465. Toxic Plasmid?? Toxic Insert?? - (reply: 2)
  466. ligation problem with SalI degested vector and insert - (reply: 6)
  467. Effect of EtBr on plasmid supercoiling - (reply: 5)
  468. Quantifying plasmid DNA - Nanodrop and Qubit (Invitrogen) suck! (reply: 2)
  469. lentivirus vector problem--help help - (reply: 6)
  470. How to know the exact plasmid size? - - supercoiled, nicked, linear (reply: 6)
  471. Digestion of a plasmid - (reply: 1)
  472. no colonies - Cloning using pENTR vector, no colonies (reply: 2)
  473. Please help Cloning issues with smaI and pUNK1 vector - (reply: 2)
  474. Retroviral vector with luciferase - (reply: 1)
  475. Problems cloning Hairpin vector - (reply: 3)
  476. Sequences of generally used plasmids? - Database for sequences available? (reply: 7)
  477. plasmid copy number - method for determination of plasmid copy number (reply: 2)
  478. Purfiying Unmethylated Plasmid - unmethylated plasmid (reply: 3)
  479. Amplifying plasmid - non specific binding of primer - (reply: 3)
  480. Storing plasmid at 4 degrees C - (reply: 5)
  481. Program to draw plasmids - draw plasmids (reply: 6)
  482. Transfer of gene from one vector to another vector - (reply: 2)
  483. Transfection of vectors - (reply: 8)
  484. CIP required for double digestion of plasmid? - (reply: 3)
  485. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
  486. Plasmid replication - Plasmid replication (reply: 13)
  487. Help with cloning a plasmid that contains repeats! - (reply: 2)
  488. Plasmid extraction sans commercial kits - (reply: 3)
  489. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
  490. Myc Tag Vector - (reply: 1)
  491. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  492. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
  493. mutations in plasmids - (reply: 3)
  494. Gel purification of vector DNA - low yield - (reply: 26)
  495. Sequential restriction digestion of plasmid - (reply: 54)
  496. Problem cloning oligo into double digested vector - (reply: 9)
  497. Big insert to put back into the vector - (reply: 17)
  498. plasmid spin column usage - (reply: 5)
  499. deleting lacZ gene from control vector - (reply: 5)
  500. Vector for conditional knockout - (reply: 1)
  501. What is the biggest insert for a viral vector? - (reply: 15)
  502. Stored bacteria lost plasmids? - (reply: 12)
  503. difference between a linear and circular plasmid/DNA for stable transfections - will a circular plasmid stably integrate into the genome? (reply: 4)
  504. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
  505. cloning problems - empty vector (reply: 3)
  506. Selection during Extraction: Plasmid or Genomic DNA? - (reply: 1)
  507. Anyone have some pEGFP-N1 or N3 vectors? - (reply: 2)
  508. PLASMID ISOLATION - (reply: 10)
  509. Ligation very large vector and small insert - It will not work... (reply: 7)
  510. Frustrated with Plasmid Preps - need help! - (reply: 14)
  511. Can't find the sequences of vector pIJ 702 - Help (reply: 1)
  512. In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)