Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Vector Related Discussions
  1. Designing primers with his-tag for pET28a+ vector - (reply: 2)
  2. a half less of plasmid copy number in the solution resulting from ddPCR than qPC - (reply: 1)
  3. Plasmid purification for Gibson Assembly - (reply: 2)
  4. Co-transfection of multiple plasmids - (reply: 1)
  5. pKD1 based vector for K. lactis expression - (reply: 2)
  6. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
  7. what‘s phage display principle - (reply: 1)
  8. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
  9. pET32b+ vector issue - (reply: 1)
  10. Are relaxed plasmids origin of rep sensitive to bp substitution in RNA I/II sequ - (reply: 5)
  11. How stable is double digested plasmid - (reply: 7)
  12. Mature miRNA in expression vector? - (reply: 1)
  13. Ligation: insert & vector are about the same size - (reply: 3)
  14. low plasmid concentration - (reply: 1)
  15. What make a plasmid candidate for stable transfection - (reply: 1)
  16. Lower Plasmid Yields - (reply: 5)
  17. Cloning - Miniprep plasmid contaminated by gDNA? - (reply: 1)
  18. Question on plasmids - (reply: 4)
  19. *NO* DNA eluted from plasmid prep - Why? - (reply: 12)
  20. pUC and pSC101 vectors in the same cell - (reply: 3)
  21. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
  22. Antisense vectors confused about construction - (reply: 1)
  23. Double Digest pCD Vector - (reply: 3)
  24. Double digestion problem with PUCsp vector - (reply: 1)
  25. How much plasmid for transformation? - (reply: 8)
  26. replicating a mammalian expression vector in bacteria - (reply: 3)
  27. preparing a vector for transfection - (reply: 2)
  28. HELP: From Plasmid Vector to Bacterial Chromosome - (reply: 2)
  29. Suitable ratio of vector and insert in cloning - (reply: 5)
  30. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
  31. When designing a Mammalian Expression Vector, Should you include the complete CD - (reply: 3)
  32. plasmid repositories - (reply: 2)
  33. How to insert PreScission protease into a vector - (reply: 1)
  34. Why the insert become shorter after inserting into plasmid? - (reply: 2)
  35. real time qPCR standard range plasmid - (reply: 3)
  36. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
  37. Cloning a big insert in a small vector - (reply: 2)
  38. How to know the plasmid extracted are indeed the plasmid of interest? - (reply: 4)
  39. Plasmids recombining - (reply: 3)
  40. Plasmid Methylation - (reply: 3)
  41. Why are there two origin of replications in plasmids - (reply: 3)
  42. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
  43. Plasmid Amplification Issue - (reply: 6)
  44. ratios insert/vector - (reply: 3)
  45. Swapping promoter of destination vector not working. Please help! - (reply: 2)
  46. IRES in expression vector - (reply: 1)
  47. Why do we create own customised plasmid rather than buying iy? - (reply: 1)
  48. Can't isolate plasmid from cloned E.coli - (reply: 4)
  49. Denatured plasmid for pcr - (reply: 1)
  50. Ligation: is it possible to ligate two vector backbones together - (reply: 11)
  51. Problems with M13 phage - (reply: 5)
  52. Screen for F plasmid in e. coli - (reply: 1)
  53. Plasmid Linearization Problems - (reply: 9)
  54. Changing the vector - (reply: 55)
  55. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
  56. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
  57. plasmid construction - (reply: 2)
  58. vector propagation in E coli - (reply: 1)
  59. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  60. Relationship between plasmid size and amount of lipofectamine needed? - (reply: 1)
  61. Can the pcDNA5/FRT/TO vector be used for transient overexpression? - (reply: 2)
  62. direction of the expression units in multi-gene vectors - (reply: 1)
  63. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
  64. ethanol precipitation plasmid DNA - (reply: 3)
  65. Plasmid storage conditions - (reply: 5)
  66. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
  67. Temperature sensitivity of Plasmids - (reply: 1)
  68. New (free) plasmid mapping program - (reply: 4)
  69. filling the digested vector and insert for blunt end cloning - (reply: 2)
  70. removing part from plasmid - (reply: 18)
  71. Help with plasmid extraction and redissolving? - (reply: 5)
  72. Nanodrop machine error message when measuring plasmid DNA concentration - (reply: 3)
  73. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  74. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
  75. Promoter sequence search in a plasmid map - (reply: 3)
  76. Heat vs pH during plasmid extraction - (reply: 8)
  77. Sterility issues with plasmid reconstitution buffer? - (reply: 3)
  78. Heating elution buffer for plasmid prep - (reply: 1)
  79. Cloning into pVIVO2 vector - (reply: 8)
  80. Cloning worked, but the expression vector is weird - (reply: 6)
  81. Using pBABE plasmids for regular transfection - (reply: 1)
  82. Rxn not working - amplify/linearize plasmid - (reply: 3)
  83. Cloning two genes in the same plasmid with one promotor - (reply: 4)
  84. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
  85. RNA contamination in plasmid extracted - (reply: 1)
  86. Problem in plasmid digestion after transformation - (reply: 4)
  87. Call for help! Plasmid extraction problem - (reply: 4)
  88. E. coli growing in LB-ampicillin medium without plasmid? Or miniprep problem? - (reply: 2)
  89. How can addgene sell plasmids with patented technology - (reply: 2)
  90. Transient transfection with large plasmid - (reply: 3)
  91. How to obtain Vectors used in articles - (reply: 5)
  92. Is it generally acceptable to ask another lab for cell line/plasmid/etc - (reply: 4)
  93. Gateway vectors - (reply: 3)
  94. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
  95. Plasmids DNA on filter paper - (reply: 1)
  96. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
  97. Kozak sequence and mammalian expression vector - (reply: 3)
  98. GFP vector - (reply: 1)
  99. Problem with Large vector & very small insert - (reply: 1)
  100. Storage life of plasmid DNA in water - (reply: 5)
  101. Insert & vector too dilute to perform ligation - (reply: 3)
  102. Plasmid DNA digestion problem - (reply: 3)
  103. Building cDNA constructs to pcDNA3.1 directional TOPO vector - (reply: 4)
  104. free online DNA & plasmid editing tool - (reply: 1)
  105. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
  106. Appearance of a salty plasmid preparation - (reply: 1)
  107. Common RE site on complementary strands in a Vector - (reply: 7)
  108. Transformation of Restricted plasmid - (reply: 3)
  109. Why is my insert missing from my plasmid? - (reply: 4)
  110. Plasmid digestion and gel purification using HindIII - (reply: 3)
  111. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
  112. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
  113. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  114. Colonies grown without plasmid - (reply: 7)
  115. Reconstituting plasmid DNA from blotting paper - water vs. TE buffer - (reply: 6)
  116. Plasmid on blotting paper - (reply: 1)
  117. Is it possible that the undigested version of this plasmid will be above the lin - (reply: 1)
  118. vector dephosphorylation - (reply: 3)
  119. What plasmid sizes do you work with for SDM? - (reply: 1)
  120. Running Plasmid DNA on Agarose Gel (Banding Pattern) - (reply: 1)
  121. Adshuttle vectors and adenoviruses - (reply: 1)
  122. Plasmid Purification Using an Alkaline Lysis - (reply: 1)
  123. cloning help; high salt level in vector - (reply: 4)
  124. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
  125. How to get rid of bacterial phage from a caterpillar surface? - (reply: 7)
  126. Running undigested plasmids on gel - (reply: 2)
  127. Plasmid isolation from Enterococcus - (reply: 1)
  128. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
  129. Software for vector sequence manipulation - (reply: 2)
  130. overexpress mature microRNA by siRNA vector - (reply: 2)
  131. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
  132. Whole plasmid amplification by PCR - (reply: 2)
  133. For promoter assay, which vector I should use? - (reply: 5)
  134. Sequencing of the cloned plasmid - (reply: 5)
  135. 4 way cloning into TA Vector - (reply: 10)
  136. Plasmid DNA contamination - (reply: 5)
  137. Problems with plasmid DNA extraction - (reply: 3)
  138. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
  139. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
  140. Can I quantify restricted plasmid and oligos? - (reply: 3)
  141. conditionally active plasmids - (reply: 1)
  142. Transient transfection of linearized plasmids - (reply: 3)
  143. Transfecting three plasmids into hES cells - (reply: 2)
  144. HUVEC plasmid transfection challenges - (reply: 2)
  145. Plasmid DNA - (reply: 3)
  146. Electroporate lentiviral vector for stable cell line - (reply: 2)
  147. Genomic DNA and plasmid DNA isolation - (reply: 3)
  148. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
  149. faint band after plasmid extraction - (reply: 7)
  150. pTRIPZ lentiviral vector induction - (reply: 1)
  151. pGEX-KG Vector insert in BL21 Codon Plus RP, Growth Difficulty - (reply: 4)
  152. plasmid extraction troubleshooting,please help !! - (reply: 9)
  153. plasmid digestion - problem with enzymes? - (reply: 4)
  154. Plasmid stability in Ecoli during storage - (reply: 3)
  155. which to use: miR or miRexpression vector - (reply: 1)
  156. Ligation with small insert and large vector - (reply: 6)
  157. Lost of Tet-on Vector - (reply: 3)
  158. Lentiviral 3rd gen transfer vector used with a 2nd generation packaging system - (reply: 1)
  159. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
  160. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
  161. Determining in frame in modified vector - (reply: 1)
  162. Creating primers to add restriction sites to vector backbone - (reply: 7)
  163. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
  164. Low copy vector for library generation - (reply: 2)
  165. Plasmid isolation from gram positive bacteria - (reply: 2)
  166. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
  167. problems with plasmid miniprep - (reply: 8)
  168. shRNA interference using Lenti virus vector - (reply: 1)
  169. Determining the amount of plasmid transfected - (reply: 1)
  170. How to check for the presence of plasmid? - (reply: 3)
  171. PCR with Plasmid recovered from filter paper - (reply: 6)
  172. Check contamination - how to sterilize vector - (reply: 1)
  173. Help for pGEM-T easy vector ligation problem - (reply: 2)
  174. pkp 59 vector - (reply: 2)
  175. Vector insert ratio in cloning - (reply: 3)
  176. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
  177. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
  178. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  179. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
  180. restricted PCR plasmid runs slower - (reply: 2)
  181. High frequency of vector religation - (reply: 10)
  182. Plasmid compatibly groups - (reply: 5)
  183. Plasmid isolation from agrobacterium - (reply: 1)
  184. Cutting my plasmid made it larger - (reply: 7)
  185. What are the "mammalian vectors N1 and C1"? - (reply: 1)
  186. Packaging vectors - (reply: 1)
  187. Recreate original plasmid by cutting out insert - (reply: 2)
  188. posting sequences of plasmid? - (reply: 3)
  189. Incomplete digestion of plasmid with single enzyme - (reply: 1)
  190. problem with plasmid digestion - (reply: 5)
  191. Protein Expression vector with small tag - (reply: 1)
  192. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
  193. unexpected band in PCR with plasmid - (reply: 4)
  194. Sequencing Primers and Plasmids - (reply: 3)
  195. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
  196. Replication of the Plasmid DNA - (reply: 6)
  197. expression plasmids for tRNA - (reply: 6)
  198. cloning pcr insert into plasmid - (reply: 2)
  199. Re-culturing empty plasmid - (reply: 1)
  200. microRNA promoter based expression vector? - (reply: 1)
  201. Can anyone recommend a good stable expression vector for human colorectal cancer - (reply: 1)
  202. help with a vector - (reply: 3)
  203. Adding T7 promoter sequence to vector - (reply: 2)
  204. Plasmid Miniprep - (reply: 2)
  205. Lipofectamin 2000 and Large Plasmid Transfection Optimization - (reply: 3)
  206. Tricistronic vector - (reply: 1)
  207. phage lysate purification - (reply: 2)
  208. Plasmid DNA at RT for a few days - (reply: 4)
  209. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
  210. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
  211. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
  212. Lentviral vectors - (reply: 1)
  213. plasmid rearrangement or sabotage - (reply: 2)
  214. pLDR20 Vector - (reply: 1)
  215. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  216. Linearising plasmid before transfection? - (reply: 2)
  217. Smear in plasmid sample - (reply: 9)
  218. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
  219. vector dephosphorylation - (reply: 5)
  220. Where can I purchase T2 or T4 phage genomic DNA? - (reply: 2)
  221. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
  222. cloning a gene in to a plasmid - (reply: 5)
  223. Characterizing an unknown plasmid - (reply: 6)
  224. Plasmid Purification and Digestion Problem - (reply: 2)
  225. PDCD4 Plasmid, RAW 264.7 not working Help - (reply: 2)
  226. QuikChange with Different plasmids - (reply: 1)
  227. Co-transfection with 2 plasmids - (reply: 10)
  228. Vector Digestion Problem - (reply: 4)
  229. Single Plasmid Gel Band - (reply: 1)
  230. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
  231. Transformation with a big plasmid - (reply: 4)
  232. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  233. Best ratio of 5 Plasmids for Lentivirus production - (reply: 4)
  234. digested plasmid - (reply: 2)
  235. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
  236. isolation of plasmid (midi and maxi prep) - (reply: 18)
  237. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
  238. How to clone insert with one sticky and one blunt end into vector? - (reply: 1)
  239. pUB Vector with two promoters - (reply: 6)
  240. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
  241. pkD46 vector growth in E.coli problem - (reply: 8)
  242. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
  243. Clean genomic DNA and plasmid DNA extraction from E.coli - (reply: 3)
  244. plasmid tetR and tet promoter - (reply: 2)
  245. Trouble in phage display - (reply: 1)
  246. Plasmid clone in BSP - (reply: 1)
  247. Swich selection in plasmid - (reply: 3)
  248. A good vector to make gene library? - (reply: 5)
  249. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
  250. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
  251. Hypothetical Synthetic Plasmid - (reply: 9)
  252. How many fragments in plasmid DNA prep? - (reply: 1)
  253. Problem with plasmids transformation - (reply: 6)
  254. Calcium chloride plasmid transfection - (reply: 1)
  255. How Much Plasmid? - (reply: 6)
  256. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
  257. Plasmid isolation - (reply: 1)
  258. plasmid;GI; ACESSION NUMBER - (reply: 2)
  259. virus transduction vs plasmid transfection - (reply: 4)
  260. How dangerous are non-viral vectors? - (reply: 3)
  261. having trouble getting insert ligation into vector - (reply: 3)
  262. Bizarre changes in plasmid band size - (reply: 3)
  263. where to get vector overexpressing caveolin-1? - (reply: 2)
  264. plasmid repository - (reply: 2)
  265. LZRS vector details - (reply: 1)
  266. transient transfection of retroviral vector without retroviral particle porudcti - (reply: 1)
  267. 12.4kb vector 6.7kb insert - (reply: 2)
  268. pGEM-T Easy vector contamination - (reply: 2)
  269. How to transfer His-tag from N- to C-terminus in plasmid - (reply: 1)
  270. High molecular plasmid isolation form kit and alkaline lysis - (reply: 2)
  271. Help: transduction of primary blood cells (T): lentiviral vs. adenoviral vector - (reply: 1)
  272. I need bynary vector pART27 - (reply: 1)
  273. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
  274. pCCL vector map - (reply: 2)
  275. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
  276. Pls help me with this plasmid!!! - (reply: 1)
  277. Standard curve using plasmid - (reply: 1)
  278. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  279. Methods of plasmid transformation? only heat shock? - (reply: 1)
  280. Cloning into inducible vector but no expression - (reply: 1)
  281. e coli vector and host selection - (reply: 1)
  282. No plasmid after a maxi prep mystery - (reply: 4)
  283. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
  284. Co-expression of 2 plasmids in E.coli - (reply: 1)
  285. How to avoid plasmid instability - (reply: 4)
  286. Plasmid vs cDNA amplification by PCR - (reply: 2)
  287. Shear-induced damage of plasmid by vortexing - (reply: 1)
  288. RNase Band in old plasmid stored at -40 - (reply: 2)
  289. Plasmid vector for yeast and E. coli - (reply: 2)
  290. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
  291. pGEX vector - (reply: 2)
  292. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  293. Filamentous phage infection - (reply: 2)
  294. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  295. how to find suitable vectors to transform wild-type bacteria - (reply: 10)
  296. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
  297. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  298. Plasmid digestion after transformation - (reply: 4)
  299. Blunt end ligation insert:vector ratio - (reply: 1)
  300. Smear above plasmid dna - (reply: 3)
  301. result of plasmid extraction - (reply: 2)
  302. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
  303. plasmid extraction - (reply: 5)
  304. agarose gel electrophoresis of plasmid extraction - (reply: 12)
  305. plasmid extraction - (reply: 3)
  306. Buy BAC vectors - (reply: 1)
  307. Is that a Plasmid??? - (reply: 5)
  308. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
  309. ask for a strain for the suicide vector called E. coli S17-1 pir - (reply: 1)
  310. plasmid extraction - (reply: 13)
  311. ligation colonies contain empty vector.. - (reply: 9)
  312. Is it posssible using shRNA vector to generate microRNA? - (reply: 2)
  313. Cloning two genes in the same plasmid - (reply: 4)
  314. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
  315. Best plasmid for GFP + Mammalian Selection - (reply: 1)
  316. Plasmid, gene orientation, insert orientation. - (reply: 1)
  317. Plasmid recovery problem - (reply: 3)
  318. Cloning problem using pET32a vector - (reply: 3)
  319. Electroporated plasmid loses the insert - (reply: 8)
  320. emty vector cloning - (reply: 3)
  321. Plasmid sequencing without primers - (reply: 1)
  322. RE site on complimentary strand of plasmid? - (reply: 1)
  323. How much of plasmid should be cut to see the gene - (reply: 1)
  324. Plasmid sequencing - (reply: 1)
  325. Plasmid isolation Qiagen - (reply: 2)
  326. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
  327. Is the T7 tag sequence immunogenic (from pET plasmid) - (reply: 2)
  328. expression vector for yeast sacchromyces cerevisiae - (reply: 2)
  329. Help in RE digestion of vector - (reply: 5)
  330. Amplify fragment in plasmid? - (reply: 4)
  331. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
  332. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  333. Gateway cloning vector compatibility - (reply: 2)
  334. gene expression in E coli with PET21a vector - (reply: 1)
  335. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
  336. Template DNA (plasmid) concentration for PCR - (reply: 4)
  337. co-expression of 2 plasmids in E.coli - (reply: 3)
  338. How to choose the right plasmids for certain types of cell? - (reply: 1)
  339. Plasmid digestion create a bigger plasmid - (reply: 1)
  340. Cloning two fragments into a vector - (reply: 3)
  341. confirmation of insert in to vector - (reply: 3)
  342. whole plasmid pcr? - (reply: 6)
  343. Looking for a GFP plasmid (non-amp) - (reply: 3)
  344. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
  345. ligation of pcr product into expression vector - (reply: 4)
  346. amp plates containing xgal/iptg with expression vector - (reply: 6)
  347. Strange Vector Problems - (reply: 3)
  348. Insert lose in Phage display library - (reply: 6)
  349. extract plasmids from transfected eukaryotic cells - (reply: 6)
  350. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
  351. Lenti vector ligation - (reply: 2)
  352. Cloning from a pGEMT Easy vector - (reply: 3)
  353. Plasmid compatible with pBAD24 - (reply: 1)
  354. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
  355. COL E1 mutations in vector after sequencing - (reply: 1)
  356. Vector with inducible/repressible system - (reply: 1)
  357. Improving expression of recomb proteins in baculovirus - (reply: 1)
  358. Cloning genes into plant vector - (reply: 1)
  359. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
  360. Difference between plasmid DNA extraction and genomic DNA extraction - (reply: 3)
  361. Recombinant construct looks smaller than empty vector - (reply: 3)
  362. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
  363. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
  364. protein expression vector.. - (reply: 4)
  365. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
  366. Lentiviral Vector - Lentiviral Vector (reply: 5)
  367. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
  368. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
  369. Best transcription vector - (reply: 1)
  370. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
  371. which is the best plasmid vector for cloning BSP products? - (reply: 2)
  372. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
  373. Cloning into lentiviral vector - (reply: 11)
  374. use of pGEM -tT easy vectors - (reply: 2)
  375. ~200kb Plasmid DNA purification is not working - (reply: 1)
  376. ligating a 6 His tag to plasmid - (reply: 2)
  377. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
  378. Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
  379. expreesing cloned vector - having problems with texpreesing cloned gene in vector. (reply: 6)
  380. Plasmid sequencing failed why? - (reply: 10)
  381. Lactic Acid Bacteria - Challenges in working with LAB culturing, cloning and plasmid (reply: 7)
  382. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  383. SLIM mutagenesis of large plasmids - (reply: 8)
  384. Introduction to vector development - Basics on vectors types and construction (reply: 1)
  385. Can you receommend a vector? - (reply: 1)
  386. GFP vector as a transfectino efficiency indicator? - (reply: 3)
  387. why 12 h culture for plasmid prep? - (reply: 2)
  388. Big plasmids and Transfection - (reply: 3)
  389. How to add a T7 terminator to a vector? - (reply: 4)
  390. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
  391. Protein expression vectors Please help - (reply: 2)
  392. vectors please help - vectors cleavage sites (reply: 1)
  393. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
  394. very small insert and a very ling vector - (reply: 1)
  395. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
  396. help: why plasmid yield dropped ever since the first culture - (reply: 2)
  397. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
  398. pcr amplify an insert in plasmid? - (reply: 2)
  399. Single-digested vector shifts downward on gel - (reply: 1)
  400. odd plasmids - (reply: 2)
  401. Question about transduction of lentivirus vector into fibroblast ? - transduction (reply: 1)
  402. Lactobacillus plasmid - (reply: 1)
  403. Will transfected plasmids induce cytokine production? - (reply: 1)
  404. Question about plasmid midiprep - (reply: 1)
  405. Plasmid Miniprep yield problems - Low rate of yield using a Miniprep Kit (reply: 10)
  406. Mirror Image Phage Display - "special" contamination (reply: 9)
  407. pGL3 vector - (reply: 3)
  408. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
  409. plasmid isolation - sth lights up in the well of agarose gel (reply: 2)
  410. Overexpression plasmid is not expressing protein - (reply: 13)
  411. Secretion cloning vectors - (reply: 1)
  412. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
  413. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
  414. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
  415. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
  416. PCR problem - basic PCR for plasmid amplification (reply: 2)
  417. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
  418. New to Gene cloning - Plasmid vector preparation (reply: 2)
  419. cloning a 34kb insert - please suggest good vectors (reply: 1)
  420. HELP is needed for low expression using pEXP5-CT vector in BL21 (DE3) plyss - low expression after time course study with 1 mM IPTG (reply: 1)
  421. How long can I store cleaved/digested vector? - (reply: 9)
  422. why cut plasmid DNA with Hind111 and EcoR1? - (reply: 1)
  423. Low yields of plasmids from miniprep - (reply: 1)
  424. A gene is not inserted into the Plasmid - (reply: 2)
  425. purification of PCR product for cloning in a vector - (reply: 3)
  426. Phage Contamination of Cultures? - Phage contamination or somethign else? (reply: 3)
  427. Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
  428. Plasmid Question - (reply: 4)
  429. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
  430. Weird migration of double digested plasmid - (reply: 4)
  431. pMX-PURO Vector not growing - (reply: 3)
  432. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  433. Help for co-transfection of proteins and plasmid - (reply: 1)
  434. subcloning into bacterial expression vector - (reply: 1)
  435. Calculating MOI of viral vector - (reply: 1)
  436. cloning into F1 ori of expression vector??? - (reply: 1)
  437. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
  438. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  439. How to store plasmid DNA on filter paper - I want to make a folder of all of them (reply: 3)
  440. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
  441. Separating cut/non-cut vector - (reply: 6)
  442. ligating small inert into a large vector - (reply: 4)
  443. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
  444. Really strange problem with sequencing and plasmid purification! - (reply: 8)
  445. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  446. plasmid DNA versus genomic DNA - concentrations (reply: 1)
  447. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  448. Undigestable/unrestrictable Plasmid? - (reply: 1)
  449. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  450. Ligation issues - Ligating a 3.3kb insert into a 15kb viral vector (reply: 1)
  451. metagenomic library consturction - BAC vectors (reply: 1)
  452. Problem in stable expression of target protein using lentiviral vector - (reply: 3)
  453. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
  454. Suitable vector backbone for in vitro translation in Dros.embryos - (reply: 1)
  455. Viruses and plasmid gaining resistance toward drugs can lead to speciatio - (reply: 3)
  456. pGEX vectors and GST expression - (reply: 3)
  457. problems encountered when working on a large plasmid - (reply: 2)
  458. expression vector with built in promoter - (reply: 1)
  459. make a Mega plasmid mutation - (reply: 1)
  460. Anyone make their own PCR cloning vector? - (reply: 5)
  461. Bicistronic vector vs "normal" vector - (reply: 5)
  462. Can't seem to get insert into vector... please help - (reply: 4)
  463. plasmid cloning - i need help.. can anyone answer me (reply: 5)
  464. Plasmid standard problem - (reply: 2)
  465. Do plasmids work in bacteria? - (reply: 1)
  466. CEN sequences on plasmids (yeast) - (reply: 4)
  467. phage DNA - DNA wont migrate on agarose gel (reply: 3)
  468. Plasmid out of BL21 for mutagensis - (reply: 3)
  469. ORI names - name of a plasmid ori (reply: 11)
  470. uncut vector contamination in gel purified band - (reply: 2)
  471. lentivirus - possible to use 2 transfer vectors? (reply: 1)
  472. Change origin of a plasmid - (reply: 4)
  473. Better to sequence from plasmid? - (reply: 5)
  474. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  475. Does a single colony of E.coli contain more than one plasmid - (reply: 7)
  476. pGL3 promoter vector - (reply: 1)
  477. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  478. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
  479. DNA extraction from induced phage - (reply: 1)
  480. How to get rid of phage contamination - (reply: 1)
  481. lambda phage library - (reply: 4)
  482. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
  483. DNA isolation from plant nuclei by plasmid extraction kit - (reply: 1)
  484. problem with pLK01 plasmid ligation - (reply: 2)
  485. Cloning long fragment...got lots of colonies with vectors that have no inserts.. - (reply: 9)
  486. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  487. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
  488. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
  489. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  490. Dephosphorylation of vector - (reply: 8)
  491. How do I actually just tag Osteosarcoma with GFP w/o using a vector - (reply: 5)
  492. Can a single colony contains more than 2 types of insert in their plasmids? - (reply: 2)
  493. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
  494. PCR mutagenesis of plasmid - (reply: 5)
  495. Guanadine HCl for plasmid miniprep - (reply: 3)
  496. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
  497. Large vector cloning-recombination problem? - (reply: 1)
  498. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
  499. Not getting plasmid from miniprep - (reply: 7)
  500. Need advice on cloning very small insert into a vector - (reply: 3)
  501. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
  502. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
  503. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
  504. Digesting large amount of plasmid - (reply: 4)
  505. Vectors with special MCS - (reply: 5)
  506. Cloning <100 bp fragments into a vector - (reply: 7)
  507. Cloning <100 bp fragments into a plasmid - (reply: 7)
  508. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
  509. Help! my plasmid size changed after ligation - (reply: 5)
  510. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
  511. Restriction digestion of gDNA for phage library - (reply: 5)
  512. mammalian expression vector - (reply: 2)
  513. Electroporation doesn't like my plasmid??? - (reply: 4)
  514. Problem with ligation - Insert into the "wrong" vector (reply: 11)
  515. Cloning in pCR2.1 plasmid - (reply: 7)
  516. No expresion of transgene - Retroviral vector (reply: 1)
  517. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  518. lambda phage library - (reply: 1)
  519. More colonies fron vector only control? - (reply: 1)
  520. empty vectors more than the insert - (reply: 6)
  521. Ligation/Transformation Troubles - Lentiviral vector (reply: 9)
  522. mutagenesis - mutate a vector by deleting its promoter site (reply: 1)
  523. phage display - peptide differentiation (reply: 1)
  524. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  525. ligation of an adaptor into a vector - (reply: 4)
  526. Rnase A for plasmid miniprep - cheaper alternative to qiagen? (reply: 4)
  527. vectors and his-tags - (reply: 3)
  528. miRNA 3´UTR target expression vectors - (reply: 6)
  529. Help cloning large insert into large vector - (reply: 7)
  530. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
  531. problem about vector Dephosphorylation - (reply: 2)
  532. Cloning: Randomly recombined Plasmids? - (reply: 6)
  533. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
  534. plasmid + sepharose - how do this work? (reply: 1)
  535. Transformation and ligation problem - Trasnformation of ligation sample shows only vector size.. (reply: 3)
  536. Anyone uses plasmid to make standard curves? how to make adjustment b/w plates - real time (reply: 4)
  537. How to elute plasmid dried on paper? - I just received plasmid samples by mail (reply: 4)
  538. are vacuum manifolds (plasmid prep) interchange with brands? - (reply: 2)
  539. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  540. Plasmid size problems! - (reply: 3)
  541. Left DNA plasmid out for a week - (reply: 3)
  542. Vector maps - (reply: 3)
  543. Transfecting primary cells with plasmid - (reply: 1)
  544. question about plasmid extraction - (reply: 2)
  545. Ligating 2 Linear fragments into a vector at Once - (reply: 4)
  546. problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
  547. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
  548. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
  549. Disappearing plasmid - (reply: 5)
  550. Transferring Plasmid from Aerobic to Anaerobic Bacteria - a thought about the oil eating microbes (OEMs) (reply: 1)
  551. Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
  552. problem in plasmid transfection - (reply: 2)
  553. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
  554. Plasmid DNA mini-prep - no good isolation (reply: 5)
  555. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
  556. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
  557. plasmid extraction - why dilute (reply: 3)
  558. Suicide vector - (reply: 2)
  559. very high molecular weight plasmid - (reply: 6)
  560. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  561. vectors maps - (reply: 2)
  562. How to dissolve phage library dry powder - (reply: 2)
  563. Fake positive colony in lamda RED reconbination, why? - (reply: 2)
  564. Fake positive colony in lamda RED reconbination, why? - (reply: 3)
  565. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
  566. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  567. Concepts behind plasmid prep - (reply: 2)
  568. help with pCMV-myc vector - (reply: 2)
  569. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
  570. PCR off plasmid for screening - (reply: 2)
  571. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
  572. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  573. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  574. Vector frameshift problem - (reply: 3)
  575. Plasmid frameshift problem after clonning - (reply: 2)
  576. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
  577. plasmid extraction kit wash buffer solution - (reply: 3)
  578. plasmid extraction kit solution composition - wash buffer (reply: 2)
  579. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
  580. are plasmids used for lab work methylated - (reply: 1)
  581. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  582. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  583. Isolation of protein crosslinked to plasmid - (reply: 3)
  584. Inseparable insert in gel purification of the vector - (reply: 3)
  585. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  586. knockdown issue...please help... - shRNA pGIPZ vector (reply: 1)
  587. Which Plasmid DNA for standard curve? - (reply: 5)
  588. pRL-TK as primary Vector ? - (reply: 1)
  589. Ligated Vector Age - When is a ligated vector too old to use? (reply: 1)
  590. GFP insert - trying to insert GFP in a vector (reply: 1)
  591. Plasmid problem - From PCR product (reply: 7)
  592. IFU, MOI, lentiviral vectors - (reply: 1)
  593. viral vector - retroviral vectors (reply: 1)
  594. Gateway and Entry Vectors. - (reply: 1)
  595. Checking PCR insert (into pGEMT vector) - (reply: 2)
  596. Transfection - Plasmid Uptake Control - (reply: 2)
  597. info about the little p in pH - i kept my promise phage!!! (reply: 4)
  598. Culture Grow well but No Plasmid - (reply: 2)
  599. software for plasmid drawing - (reply: 13)
  600. Empty TOPO expression plasmid - and lab-made TOPO vector! (reply: 4)
  601. Ligation and Transformation help needed: pENTR1A Vector issue - (reply: 5)
  602. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
  603. plasmid purification kit for transfection - (reply: 2)
  604. best plasmid purification kit for transfection - (reply: 1)
  605. Expression problem using invitrogen pRSET C vector - (reply: 5)
  606. Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
  607. Which pGL4 vector to choose - (reply: 2)
  608. Vector containing N-his tag and TEV - (reply: 1)
  609. LR reaction - Cloning into a expression vector (reply: 1)
  610. Vector map for pMH1 - Can't find it anywhere! (reply: 2)
  611. Checking Plasmid Expression - (reply: 1)
  612. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  613. about pre-miRNA cloned into lentivirus vectors - (reply: 4)
  614. qPCR determination of phage numbers - slope difference for different phage clones (reply: 2)
  615. PCR a plasmid protein - (reply: 2)
  616. plasmid DNA disappear in the minipre - (reply: 2)
  617. Interpreting a Plasmid Profile gel photo - (reply: 3)
  618. AdEasy Vector System: Why PacI digestion? - (reply: 1)
  619. marker swap plasmid - (reply: 4)
  620. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
  621. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
  622. plasmid dna isolation - (reply: 1)
  623. Vector needed - (reply: 1)
  624. having the wrong vector in my clone - (reply: 3)
  625. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  626. siRNA and plasmid cotransfection - (reply: 1)
  627. rna from plasmid - (reply: 1)
  628. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
  629. Problems facing in cloning with pBI121 vector - (reply: 1)
  630. design parameters for multiple siRNA expression in vector - (reply: 4)
  631. Plasmid curing method - (reply: 4)
  632. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  633. Co-transfection of miRNA and vector expressing target sequence - (reply: 2)
  634. religate the vector - need good protocol! - (reply: 4)
  635. plasmid DNA lab question - (reply: 11)
  636. ShRNA vectors for other than mammalian systems - shRNA for S. pombe (reply: 6)
  637. Problem amplifying plasmid - (reply: 1)
  638. Can linear vectors be transformed and can they express resistance genes? - (reply: 4)
  639. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
  640. Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)
  641. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
  642. HELP! Can't double digest pcDNA3.1 vector - (reply: 6)
  643. Help complementing my mutant in trans - help me find a plasmid (reply: 1)
  644. What about the Baculovirus expression system? - (reply: 2)
  645. Vector NTI from Invitrogen - (reply: 4)
  646. role of glucose in plasmid isolation? - (reply: 1)
  647. Plasmids for protein expression in mammalian cells - (reply: 2)
  648. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
  649. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
  650. Amplfy vector - How to amplify vector (reply: 2)
  651. after re-isolation of vector, get single band - (reply: 4)
  652. Cloning big fragment into smaller vector - (reply: 3)
  653. from where can i get the P1 phage - (reply: 1)
  654. Ligation/Transformation problems & 260/230 ratio of vector - (reply: 4)
  655. plasmid, BAC, or cosmid? - (reply: 3)
  656. Ligation of digested plasmid to hairpin oligo - (reply: 5)
  657. Nucleospin Extract II kit also for plasmids? - (reply: 5)
  658. Best plasmid miniprep? - (reply: 13)
  659. Plasmid instability in E.coli BL21? - (reply: 3)
  660. Difficulty cloning vector with multiple Lox sites - (reply: 2)
  661. Working gene-coding plasmid does not produce transcript or protein - (reply: 2)
  662. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
  663. Vector sequence included in (PCR) amplified insert - (reply: 2)
  664. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
  665. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  666. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
  667. Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL - (reply: 1)
  668. pCLBABE puro retriviral vector - (reply: 1)
  669. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
  670. Plasmid DNA sequence and map - PLASMID DNA (reply: 2)
  671. Plasmid linearization by PCR - (reply: 4)
  672. plasmid sequence identification problem - (reply: 2)
  673. Best lentiviral vector - (reply: 2)
  674. Suitable bacterial reporter vectors - (reply: 3)
  675. plasmid construct problem - (reply: 3)
  676. Problem proving insert is in vector :( - This has been going on for months :( (reply: 5)
  677. Transformation problem. Please help! - 1.7kb insert to 5.4kb vector; double digested w/ EcoRV & XhoI (reply: 17)
  678. Transient expression vector - (reply: 11)
  679. How to get plasmid maps from publications? - (reply: 5)
  680. pMSP12-GFP map? - How to get plasmid maps. (reply: 1)
  681. Salmonella vector- non-ColE1 - Suggestion for good Salmonella vector? (reply: 7)
  682. A gateway cloning expression vector question - (reply: 1)
  683. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
  684. File format for plasmids in Vector NTI, Clone Manager etc - I'd like a compatible format with no/minimal data loss (reply: 3)
  685. Ligation Failed, too little Vector??? - Ligation trouble shooting (reply: 5)
  686. Low level of expression - in pASK-IBA7 vector (reply: 6)
  687. How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
  688. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  689. Choice of expression vector - how to choose an expression vector (reply: 1)
  690. New Plasmid Draw/Plotter Program (Free) - (reply: 4)
  691. Plasmid DNA isolation - neutralisation of DNA (reply: 5)
  692. Why the fluoresence is dim or dispear in miRNA vector? - miRNA (reply: 3)
  693. Phage removal of E.coli - (reply: 8)
  694. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
  695. Vector with 2 promoters to drive expression of 2 genes - Any recommendations? (reply: 3)
  696. Luciferase reporter induced by betagal vector?? - (reply: 1)
  697. PCR problem from transformed TOPO TA vector - (reply: 3)
  698. Struggling for months with one little ligation/transformation - please help! - XhoI digested 1,5 kb insert needs to be ligated into 4,8kb vector (reply: 11)
  699. Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
  700. Self-Ligation of digested vector - (reply: 2)
  701. Ligation issue: Does vector size matter? - (reply: 10)
  702. Plasmid sequence - (reply: 1)
  703. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
  704. Promoters and vectors for shRNA? The need for specific vectors and promoters? - (reply: 4)
  705. Mammalian expression vectors with HA, c-myc, FLAG or anyother tag - The lab is running out of funds and need some cheap source. (reply: 3)
  706. What is stringent vector? - Is it related to level of expression? (reply: 2)
  707. expression vector in E. coli - the best expression vector in E. coli (reply: 5)
  708. pBMN-GFP as episomal vector - (reply: 1)
  709. where can i ontain the binary vector pCB301? - (reply: 1)
  710. plasmid isolation - (reply: 4)
  711. Plasmid supercoiling affecting PCR? - (reply: 2)
  712. Miniprep / plasmid question - How long can you get plasmid? (reply: 5)
  713. TOP10F' - Can they be used for regular vector propagation? (reply: 1)
  714. Cloning done....sequence of the insert for the expression vector - (reply: 1)
  715. heterodimer - bicistronic vector? (reply: 2)
  716. looking for a vector with a Neo cassette, HindIII and BamHI restriction sites (i - (reply: 7)
  717. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  718. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
  719. problem with plasmid extraction - (reply: 3)
  720. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
  721. How to transfect two plasmids in a cell - (reply: 10)
  722. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
  723. How to separate large size plasmids ~12kb - (reply: 8)
  724. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  725. Vector NTI users!!!! please read this!!! - free alternative to vector nti (reply: 10)
  726. low plasmid yield from midi preps - (reply: 4)
  727. Plasmid isolation, help with dilution step? - (reply: 7)
  728. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
  729. full digestion of plasmid to get only dNTPs - (reply: 3)
  730. Lenti viral Vector with GFP - (reply: 4)
  731. inducible shRNA vector system - (reply: 7)
  732. size limit of plasmid origins - (reply: 2)
  733. Creating empty vector by self-ligation - (reply: 31)
  734. How to increase the concentration insert or vector or no cut plasmid - How to increase the concentration insert or vector or no cut plasmid (reply: 4)
  735. confusion with different forms of plasmid - (reply: 3)
  736. pQE vector protein expression - (reply: 3)
  737. zebrafish gene regulation can I use mammel expression vector? - (reply: 1)
  738. Plasmid as standard - Plasmid calculation (reply: 6)
  739. Toxic Plasmid?? Toxic Insert?? - (reply: 2)
  740. ligation problem with SalI degested vector and insert - (reply: 6)
  741. Effect of EtBr on plasmid supercoiling - (reply: 5)
  742. Quantifying plasmid DNA - Nanodrop and Qubit (Invitrogen) suck! (reply: 2)
  743. lentivirus vector problem--help help - (reply: 6)
  744. How to know the exact plasmid size? - - supercoiled, nicked, linear (reply: 6)
  745. Digestion of a plasmid - (reply: 1)
  746. no colonies - Cloning using pENTR vector, no colonies (reply: 2)
  747. Please help Cloning issues with smaI and pUNK1 vector - (reply: 2)
  748. Retroviral vector with luciferase - (reply: 1)
  749. Problems cloning Hairpin vector - (reply: 3)
  750. Sequences of generally used plasmids? - Database for sequences available? (reply: 7)
  751. plasmid copy number - method for determination of plasmid copy number (reply: 2)
  752. Purfiying Unmethylated Plasmid - unmethylated plasmid (reply: 3)
  753. Amplifying plasmid - non specific binding of primer - (reply: 3)
  754. Storing plasmid at 4 degrees C - (reply: 5)
  755. Program to draw plasmids - draw plasmids (reply: 9)
  756. Transfer of gene from one vector to another vector - (reply: 2)
  757. Transfection of vectors - (reply: 8)
  758. CIP required for double digestion of plasmid? - (reply: 3)
  759. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
  760. Plasmid replication - Plasmid replication (reply: 13)
  761. Help with cloning a plasmid that contains repeats! - (reply: 2)
  762. Plasmid extraction sans commercial kits - (reply: 3)
  763. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
  764. Myc Tag Vector - (reply: 1)
  765. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  766. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
  767. mutations in plasmids - (reply: 3)
  768. Gel purification of vector DNA - low yield - (reply: 26)
  769. Sequential restriction digestion of plasmid - (reply: 58)
  770. Problem cloning oligo into double digested vector - (reply: 9)
  771. Big insert to put back into the vector - (reply: 17)
  772. plasmid spin column usage - (reply: 5)
  773. deleting lacZ gene from control vector - (reply: 5)
  774. Vector for conditional knockout - (reply: 1)
  775. What is the biggest insert for a viral vector? - (reply: 15)
  776. Stored bacteria lost plasmids? - (reply: 12)
  777. difference between a linear and circular plasmid/DNA for stable transfections - will a circular plasmid stably integrate into the genome? (reply: 4)
  778. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
  779. cloning problems - empty vector (reply: 3)
  780. Selection during Extraction: Plasmid or Genomic DNA? - (reply: 1)
  781. Anyone have some pEGFP-N1 or N3 vectors? - (reply: 2)
  782. PLASMID ISOLATION - (reply: 10)
  783. Ligation very large vector and small insert - It will not work... (reply: 7)
  784. Frustrated with Plasmid Preps - need help! - (reply: 14)
  785. Can't find the sequences of vector pIJ 702 - Help (reply: 1)
  786. In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)