Suicide vector - (May/02/2010 )

Hi,

I'm trying to clone a PCR product into a suicide vector which would hopefully inactivate a specific gene through recombination. My vector has 2 multiple cloning sites... Does anyone know if the cloned gene orientation matters? Should it come before or after the antibiotic marker?

Thanks !

It shouldn't matter. How large are your recombination sites?

phage434 on May 2 2010, 06:52 AM said:

Hi,
About 1kb. I plan to clone different restriction sites for the ends to ensure the correct orientation in the vector, but I just wasn't sure about the antibiotic marker.