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plasmid more than recommended for transfection - will it be toxic? (Aug/28/2010 )

Is higher amount of plasmid toxic to the cells in transfection? Why is there a limit for plasmid concentration in transfection?

Because I'm getting pretty better results when using more plasmid than recommended. I get >80% efficiency in 10cm well of HeLa cells when I use 10ug of pEGFP.

-Curtis-

anyone?

-Curtis-