Change origin of a plasmid - (Nov/02/2010 )
Hi,
can I simply change a origin of replication from one plasmid by cutting the old one out and insert a new one from another plasmid?
Can I even change antibiotic resistance or must I regard something special to not disturb the plasmid structure?
Thanks in advance
Mara
-MaraJade-
It's all just DNA. Do it.
-phage434-
Changing antibiotic resistance is not a big deal. when u change the origin of replication, take care of the upstream and downstream of the gene,it may interfer in the replication.
MaraJade on Tue Nov 2 09:54:57 2010 said:
Hi,
can I simply change a origin of replication from one plasmid by cutting the old one out and insert a new one from another plasmid?
Can I even change antibiotic resistance or must I regard something special to not disturb the plasmid structure?
Thanks in advance
Mara
-christy-
Thx
-MaraJade-
phage434 on Tue Nov 2 11:05:42 2010 said:
It's all just DNA. Do it.
Wow,
this will be definitely another classical inspirational quote for molecular biologist after Nike's "Just do it".
-adrian kohsf-