use of pGEM -tT easy vectors - (Jul/06/2011 )

I have been trying to ligate my product into T easy vector . but the clones don seem to come at all. tried changing competent cells . its still not working .
my PCR products have been made by Pfu polymerase which has high fidelity and does not add A in the end therefore i did A tailing for them . what could be the prob ?

Just try once: use normal Taq, do the PCR again, and try ligate again into your vector. If this succeed, this means you might did sthg wrong during the A-tailing procedure. If not, it could be your vector/ligation problem.

Just 2 cents.

Just try once: use normal Taq, do the PCR again, and try ligate again into your vector. If this succeed, this means you might did sthg wrong during the A-tailing procedure. If not, it could be your vector/ligation problem.