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Plasmid storage conditions - (Jun/23/2014 )

Is it still safe to use a plasmid kept in water in the 4C fridge for 1 year?

-science noob-

Safe for what? - you shouldn't catch any illnesses from it so long as it isn't visibly growing something,

 

The only way to tell if it is useable is to run some on a gel and look at its integrity.

 

Depending on application, you might be better off transforming and doing a fresh prep.

-bob1-

yes, check it's integrity on a gel. it may have been acid hydrolyzed by the water (after absorbing co2 from the air).

-mdfenko-

If it was stored in TE rather than water, I'd say it is likely fine. If in water, then I would have doubts. Almost certainly you could transform it, which is what I would do immediately, then do a new prep.

-phage434-