Antisense vectors confused about construction - (May/27/2015 )
When constructing antisense vectors for gene silencing, I'm a bit confused about the antisense sequence.
Do I just need to reverse complement this as if I was making a reverse primer?
Or am I just supposed to complement it?
What would happen in either case - which one binds with the mRNA produced from the sense sequence?
-Jones1233211-
It is essentially NEVER correct to use the complement nstead of the reverse complement. Pretend you are designing the reverse primer.
-phage434-