Checking Plasmid Expression - (Dec/04/2009 )
I have a plasmid with dual T7 promoters to express both strands of a 300 bp insert.
I transformed BL21 with this construct and I want to make sure that both strands are being expressed.
Is there any way to know if expressed RNAs are staying as 2 separate single strands or forming a double strand?
run it on a gel before and after heat denaturation?