Promoter sequence search in a plasmid map - (May/09/2014 )
I have cloned my insert into a vector. I am told that the position the insert is in will be flanked by an upstream promoter (which is necessary for to express the gene).
But I can't see the promoter-annotation in the plasmid map at all (I have no annotations at all that there is an upstream promoter), which makes me question if this vector is correct.
I am told that I can BLAST the whole construct-sequence, but I get a lot of hits that doesn't make sense to me. Can anyone provide a simple guidance to check if I have a promoter in my vector?
well, whats the sequence?
(of the entire plasmid)
This tool will annotate some elements frequently found in plasmids. Of course, if you have a specific promoter, it may not indicate it but it's worth a quick try: