help with a vector - (Dec/01/2012 )
I just started grad school the beginning of this past semester, and I am relatively new to the site also. Anyway, I just started a cloning project and my p.i. told me to use a specific plasmid to use. The issue I am having is that I cannot find a map of the plasmid pJDC15. I have the papers where its construction is described but I have no idea where to actually find a restriction map. Does anybody have any kind of leads or know where I can find it?
Also, I was wondering if anybody had any sage wisdom they would like pass on to me in regards on how to survive grad school..
Thanks in advance and I hope you have good day..
You will probably have to reconstruct the exact sequence from the sequences of the component parts. It may be easier (and more reliable) to sequence the plasmid. I'd recommend that approach.
I think I will just have it sequenced. That will probably be the most reliable method. I might be able to reconstruct it from the paper I found for now. Then when I get it sequenced I will go from there..
Thank you for your help!
If this is an important plasmid in your area of research, you could consider depositing its sequence in Genbank. But be aware that you can't also put in a reference to its use because Genbank won't allow that, since you are not the original source of the plasmid. The next person working on th plasmid will thank you, however.