Protocol Online logo
Top : New Forum Archives (2009-): : Protein and Proteomics

How to transfer His-tag from N- to C-terminus in plasmid - (May/06/2012 )

Hi Guys

I was wandering about one of my protein that I want to change the His-tag from N-terminal end to C-terminus with thrombine site. I don't know how to do it. Could some one help me which the easiest/quickest way to do it.

Thanks a lot


The easiest will be to subclone into a vector with a c-terminal tag, or to PCR out the gene using primers that include the His tag sequence.