Plasmid Purification Using an Alkaline Lysis - (Sep/14/2013 )
think about the procedure you used what could have caused nicked and coiled form of the plasmid to be present?
-Phoebe Dinh-
This is obviously homework - so a bit of input from you before we answer would be good.
What do you know about alkaline lysis preps? What are the steps and what does each step do?
-bob1-