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Plasmid Purification Using an Alkaline Lysis - (Sep/14/2013 )

think about the procedure you used what could have caused nicked and coiled form of the plasmid to be present?

-Phoebe Dinh-

This is obviously homework - so a bit of input from you before we answer would be good.


What do you know about alkaline lysis preps? What are the steps and what does each step do?