Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Biology

Myc Tag Vector - (Mar/02/2009 )

I need to clone a gene and transfect into human cell line,

I need to tag my protein with Myc tag (N or C terminal both ok)

And express with a CMV promoter

Hopefully I want to insert the gene into the vector using the cutting site: 5' EcoRI, 3' SalI

Does anyone know of a vector that I can use?


One could PCR amplify the gene with the myc tag.