Amplfy vector - How to amplify vector (Sep/23/2009 )
Hi. I have 20ul of precipitated vector that we obtained from another lab. i wish to amplify and have more of the vector. Can just transform it with competent Ecoli cells and purify doing a miniprep and then a Maxi? or is there any other quick technique to obtain more of the vector?
Dilute some of the vector to 1-2 ng/ul and transform in competent cells and perform a maxiprep. When setting up your culture for the maxiprep, follow the appropriate protocol based on the plamid copy number. Confirm the identity of your plasmid by restriction digestions and sequencing! Many labs have sent me plasmids that were not what they were supposed to be or had mutations. To save yourself time, energy, and resources, SEQUENCE any plasmid sent to you by anyone before you test it experiementally!
Thanks Dr. teeth on that suggestion
Dr Teeth on Sep 23 2009, 12:50 PM said: