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plasmid copy number - method for determination of plasmid copy number (Mar/19/2009 )

Hiiii

I want to determine plasmid copy number in one popular strain of E.coli. Currently I am using fluoresence method in corelation with cfu/ml of the culture. It is a tediously long procedure as well as accuracy is not so good. I found huge variation every time.Can anybody please tell me some quick and accurate method of determination of plasmid copy number?

Thanks

-somu-

somu on Mar 20 2009, 12:13 PM said:

Hiiii

I want to determine plasmid copy number in one popular strain of E.coli. Currently I am using fluoresence method in corelation with cfu/ml of the culture. It is a tediously long procedure as well as accuracy is not so good. I found huge variation every time.Can anybody please tell me some quick and accurate method of determination of plasmid copy number?

Thanks



Real-time PCR will be helpful in this context. Maybe u can read the following journal:

Quantitative real-time polymerase chain reaction for determination of plasmid copy number in bacteria
Chai Lian Lee, Dave Siak Wei Ow, Steve Kah Weng Oh *
Journal of Microbiological Methods 65 (2006) 258 267

Lactolee

-lactolee-

Thank you.
Attached File

-somu-