gene expression in E coli with PET21a vector - (Nov/20/2011 )
Hi everyone, I have a problem with my gene and i was wondering if anyone could help me. I am working on a beta glucosidase gene. i want to express it in e coli. i cloned my gene into an amplification vector but i designed my primers that doesn't include the signal peptide of the gene. So there is no start codon in my primers. Do u think must i design new primers or continue to clone it to opet21a and express.
In case of PCR you wouldn't need signal peptide as it would cause misleading results.