can you recommend a gateway mammalian expression vector? Please? :) - (Jun/12/2012 )
Hi all,
I'm wanting to generate some stable cell lines expressing my gene of interest.
I plan to use BALB/c 3t3 fibroblasts.
I have the gene of interest as pDONR entry clones, and am about to go ahead and generate expression clones using pCDNA3-DEST. My PI has failed with this in the past which got me thinking...
Is there anything better available? What do you use, what would you suggest?
Any ideas and help appreciated.
Thanks!
-leelee-
Bump.
Yep I've been distracted with other things, but I'd like to try this now... so any suggestions?
-leelee-