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different orientation or direction of genes in expression Plasmid vector - (Nov/10/2012 )

Dear all,

Wanna Help, Here's an example I'd use to ask my questions regarding how genes are expressed in a plasmid. In the pBR322 below, I’ve noticed that there are two antibiotic resistance markers going in opposite directions. Also, the origin is going in the counter clockwise direction. Could you please let me know now I'm using tetracycline to select for my transformant, how'd the TetR gene be read since it’s on a collision course with the ori?
Attached Image

-Akila Wijerathna Yapa-

As I think this is a homework question which you should be thinking about yourself - think about the function of each of the parts you are looking at - the ori is the origin of replication, Tetr is a gene....

-bob1-

The answers to your 'question' can be found here http://www.protocol-online.org/forums/topic/13952-antibiotic-resistance-genes-in-a-plasmid/

-Julio-Claudian-

Dear

bob1 on Sun Nov 11 03:56:07 2012 said:


As I think this is a homework question which you should be thinking about yourself - think about the function of each of the parts you are looking at - the ori is the origin of replication, Tetr is a gene....


Dear bob1;

Thank you

-Akila Wijerathna Yapa-

Julio-Claudian on Sun Nov 11 04:03:01 2012 said:


The answers to your 'question' can be found here http://www.protocol-...s-in-a-plasmid/


Dear Julio-Claudian;

Thank you very much for your kind replyn

-Akila Wijerathna Yapa-