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Gene Related Discussions
  1. no amplification in target gene - (reply: 1)
  2. Compare the expression of two target genes - (reply: 3)
  3. gene cloning questions - (reply: 3)
  4. need an example of a gene with a drug target.... - (reply: 1)
  5. House keeping gene copy number - (reply: 3)
  6. expression level comparison - a database to find gene expressi (reply: 2)
  7. "Gene expression vs Transcription vs mRNA levels": Help with conceptua - (reply: 1)
  8. gene expresion - (reply: 1)
  9. tagging of gene with GUS gene - (reply: 1)
  10. Gene complexity and protein complexity analysis - (reply: 2)
  11. DeltaDelta Ct Relative or Comparative Quantitation - methods of relative quantitation for qPCR determining gene expression (reply: 3)
  12. Why DNA sequencing does not detect deletions - help! - Med student lost on her hw. Breast cancer gene (BRCA1) mutations (reply: 3)
  13. can shRNA expression set share one polyA terminator with other gene - (reply: 3)
  14. Gene onthology software - (reply: 1)
  15. Tool to map genes on chromosomes - (reply: 3)
  16. mRNA Expression - How to observe mRNA expression of gene. (reply: 10)
  17. Looking for nucleotide sequence of rearranged antibody gene locus. - (reply: 1)
  18. Gene Knockout - (reply: 2)
  19. Best chemistry for relative quantitation of gene expression - Give your opinion on the best chemistry according to your experience (reply: 5)
  20. gene expression analysis - (reply: 1)
  21. housekeeping genes for brain tissue - (reply: 4)
  22. gene dowregulation by miRNA - (reply: 1)
  23. house keeping gene not intron spanning and sample DNase UNtreated - (reply: 1)
  24. B-catenin pathway - Lef/TCF-sensitive reporter gene assay (reply: 4)
  25. Help: JIGSAW- gene prediction - (reply: 1)
  26. Bulk extraction of genes information - (reply: 1)
  27. Identification of weakly expressed novel gene - (reply: 1)
  28. Bacterial gene disruption problem - (reply: 2)
  29. Can we measure gene expression with standard PCR? - (reply: 1)
  30. Can i use old standard curve to determine gene concentration? - (reply: 1)
  31. Cutting out EGFP gene from pEGFP-N1 - w/o using XbaI (reply: 2)
  32. qPCR - running taqman for endogenous control and SYBR for the gene - (reply: 2)
  33. housekeeping gene for B cell lines - (reply: 1)
  34. incorporation of restriction site in Primers - Help to design primer to clone gene in frame with fluorescent tag (reply: 2)
  35. lux gene cloning using Sal I as restriction enzyme - (reply: 1)
  36. how to check the insertion of a gene fragment into plasmid - (reply: 2)
  37. I want to find the promoter sequence of human IL-1 beta gene - (reply: 1)
  38. gene copy number - how to find how many? (reply: 12)
  39. Analyzing microarray data: a tool for annotations and grouping genes? - (reply: 2)
  40. Interpreting PCA Graph for Gene Expression - Principle Component Analysis in Gene Expression (reply: 1)
  41. RNAi of two different genes - (reply: 4)
  42. Finding correlated genes between microarray data sets - (reply: 3)
  43. which part of gene is needed for normal expression in vector - (reply: 2)
  44. How to target a gene for deletion - (reply: 2)
  45. how to check organ(tissue)-specific expression profile of a gene - (reply: 3)
  46. housekeeping gene for comparing gene expression between tumor and normal tissue - (reply: 1)
  47. How to identify novel gene using bioinfomatics - (reply: 3)
  48. PCR on this gene - products always present? - (reply: 9)
  49. Gene Deletion in E.Coli Bl21 - (reply: 8)
  50. what are 'floxed' genes in interbred mouse - (reply: 1)
  51. Does kanR gene confer resistance to Ampicillin? - (reply: 1)
  52. Siginificant Homology at the Amino acid level? - Gene sequence homology (reply: 1)
  53. Techanical Question about cloning of gene - (reply: 2)
  54. Ampiciline resistance gene - (reply: 1)
  55. Amplifying large gene - (reply: 2)
  56. NCBI gene sequence SOURCE? - (reply: 7)
  57. confirming how the gene causes decreased growth/increased cell death - (reply: 4)
  58. Confirmation of protein expression in pCDNA3 - How to know foreign gene expression in pCDNA3 (reply: 8)
  59. Real time pcr in Rotor gene 6000- not getting positive results - (reply: 1)
  60. gene duplication - affected by siRNA? (reply: 1)
  61. Housekeeping genes for rat brain - (reply: 4)
  62. Chromosomal Gene Deletion - Gene Deletion by Double Crossing Over in Pseudomonas (reply: 6)
  63. Real-Time PCR and Housekeeping gene - (reply: 9)
  64. gene from 4-TOPO to expression vector - (reply: 1)
  65. help!real-time PCR to see gene's expression after treatment with cytokin - (reply: 4)
  66. New gene insertion in yeast - What are the suitable loci? (reply: 3)
  67. gene prediction tools - (reply: 1)
  68. histone modification-ChIP analysis - selection of proper internal control for repressed state of gene (reply: 2)
  69. Is this gene going to express? - is only the firs ATG read and express or all 3 reading frame? (reply: 10)
  70. gene hunting advice - (reply: 2)
  71. Genorm - Validation of housekeeping genes - (reply: 1)
  72. Can you get two proteins made from a heterozygous gene mutation? - (reply: 3)
  73. Affymetrix chip - unexpressed genes, which signal cuttof to use - (reply: 1)
  74. growing after filling in monolayer, please help - How and which genes/growth factors do it (reply: 5)
  75. gene was amplified when -RT were used as template? - (reply: 4)
  76. Cloning 2kb+ gene by PCR - Is it doable? (reply: 3)
  77. How to find miRNA precursor (or gene) for a specific miRNA - (reply: 2)
  78. primers dont match FASTA gene sequence... 'm confused - (reply: 11)
  79. BSP to test imprinted genes DNA methylation of oocytes - no specific or smear bands! (reply: 4)
  80. How to amplify 4.1Kb gene from genomic DNA? - (reply: 15)
  81. How to find promoter region of rat gene? - (reply: 2)
  82. comparing difference in gene expression - (reply: 2)
  83. software for CpG island prediction for a list of genes - (reply: 3)
  84. quantifying my gene expression in 5 different cell lines - (reply: 1)
  85. Does a gene have an associated CpG island? - (reply: 9)
  86. Finding a target gene sequence in rat genome using a mice gene - How to find the delta opioid receptor gene in rat if the sequence is k (reply: 2)
  87. Cloning toxic gene - (reply: 2)
  88. Scramble siRNA shows increase in gene expression by Taqman RTPCR - (reply: 1)
  89. Gene expression - (reply: 1)
  90. how to compare promoter regions from two genes - (reply: 2)
  91. neomycin-resistant gene vs kanamycin - (reply: 3)
  92. porcine CD4 gene sequence needed - urgently - (reply: 2)
  93. how to switch to a gene in a vector using PCR or restriction enzymes? - gene is in pIVS2 vector (reply: 3)
  94. Advice on analysing Differentially Methylated Regions of a gene. - (reply: 5)
  95. Genes/Proteins - (reply: 6)
  96. how to compare 2 different gene expression level in the same sample? - can I use ratio(A/B)=2^(CtB-CtA)to calculate these two genes' expr (reply: 5)
  97. How to find promoter region between two closely placed genes - (reply: 1)
  98. gene disruption - should regions flanking a cassette be of equal size (reply: 2)
  99. Induce mutation in my gene - (reply: 5)
  100. reporter gene fusion - (reply: 3)
  101. Bacterial housekeeping genes - What genes are commonly used as housekeeping controls in expression st (reply: 1)
  102. Reference Gene: human leukocytes - (reply: 2)
  103. rrn gene copy numbers - (reply: 1)
  104. Maximum acceptable variation of Housekeeping gene - (reply: 1)
  105. detection of mec A gene - (reply: 1)
  106. pcr for gene fusion - (reply: 3)
  107. Imprinted genes as a control? - (reply: 1)
  108. mutation in a gene that is not expressing a protein - (reply: 4)
  109. Long arm short arm of a gene/RF, not chromosome - (reply: 1)
  110. impossible gene ! - (reply: 3)
  111. integration vector question - do i need to clone a full gene (reply: 1)
  112. About housekeeping gene - (reply: 3)
  113. housekeeping genes - (reply: 1)
  114. Database of embryonic expressed genes - (reply: 2)
  115. problem with -ve controls of mt genes - (reply: 1)
  116. Need a weak promoter for reporter gene assay - (reply: 3)
  117. Cloning incomplete genes - (reply: 2)
  118. How to add a lethal gene to my plasmid for cloning selection? - (reply: 3)
  119. transcription of the genes in one operon - (reply: 1)
  120. ways to get a good gene amplification - (reply: 4)
  121. can cells just stop having the gene? - (reply: 4)
  122. how to amplify a long gene? - (reply: 3)
  123. Quorum sensing gene cloning - (reply: 7)
  124. Gene body methylation and active gene expression - (reply: 6)
  125. Replacing small gene with large gene - (reply: 2)
  126. Strategies for gene cloning and expression - Discuss various methods for it (reply: 8)
  127. two additional amino acids in gene - (reply: 4)
  128. Excision of foreign genes by E.coli - suspicion of attack (reply: 5)
  129. does size matter - how many genes in 1 vector (reply: 10)
  130. RNAi targeting one gene in a bicistronic transcript - Will the other gene be knock down? (reply: 4)
  131. RFLP versus a single gene to study genotypic diversity - (reply: 4)
  132. how to find the gene promoter region? - (reply: 1)
  133. how to get specific amplfication of a gene that is extremely similar to another - (reply: 7)
  134. How long does it take for the gene to lose? - (reply: 2)
  135. cDNA for real time PCR - for gene expression study (reply: 1)
  136. Creating Multimer Genes, cloning - cloning (reply: 1)
  137. Cloning of gene from peptide sequence - (reply: 1)
  138. transfer gene to protein? - (reply: 3)
  139. Used linear concatameric genes for transient transfection? - (reply: 2)
  140. how to know/predict a specific gene is regulated by miRNA - (reply: 7)
  141. PCR'ing a high GC gene - (reply: 1)
  142. which is the best approach for simultaneous siRNA of two genes? - (reply: 4)
  143. SDS page and gene cloning - pBS plasmid (reply: 1)
  144. subcloning dengue NS1 gene problems - molecular biology, cloning (reply: 5)
  145. Is it absolutely necessary to do BGS before doing MSP for new genes? - (reply: 4)
  146. GAPDH endogenous control gene - (reply: 2)
  147. Real Time PCR and Gene expression - (reply: 1)
  148. Inserting promoters upstrem of reporter genes - (reply: 2)
  149. where can I get any plasmid with aadA as selective marker gene? - i might offer some money (reply: 1)
  150. gene of interest or housekeeping gene shift...? - (reply: 3)
  151. Why is it difficult to PCR taget gene in rice successfully ? - (reply: 1)
  152. Express miRNA in vector for gene knockdown - (reply: 4)
  153. Relative expression of genes between bacterial species - (reply: 1)
  154. luciferase reporter gene assay - (reply: 3)
  155. RT-PCR of apoptosis related genes - (reply: 2)
  156. Is all lab bacterial strains have t7 RNA polymerase gene... - (reply: 4)
  157. amount of DNA for low copy number gene - (reply: 1)
  158. bacteria gene knockout using Red recombination system - (reply: 1)
  159. Reference genes on same plate as gene of interest? - (reply: 2)
  160. PCR clone a gene with known 3' end only - (reply: 4)
  161. where to find the list of genes known to be methylated - (reply: 2)
  162. semi-qRT-PCR - problem with housekeeping gene (reply: 1)
  163. change in expression of House keeping gene - change in expression of House keeping gene (reply: 2)
  164. primer dimer in pcr product - cloning for a gene. analyzing pcr product shows primer dimer (reply: 8)
  165. Replacing of GFP cloned inside a gene with RFP - (reply: 2)
  166. Problem PCR amplifying gene promoter regions - (reply: 4)
  167. official database for cancer-related genes - (reply: 3)
  168. how the gene cloned into a vector can expressed in mammalian cell without the 5& - What is the mechanism of translation initiaition with the HCM promoter? (reply: 4)
  169. 3'UTR and Gene expression in expression construct - (reply: 2)
  170. qPCR with Corbett Rotor Gene 6000 - (reply: 3)
  171. PCR analysis of clones - Can a gene specific primer pair amplify a fragment in the wrong direction? (reply: 1)
  172. induction estrogen responsive gene (PS2 & PgR) in MCF7 - (reply: 3)
  173. co-transfection - co-transfection affect gene expression? (reply: 4)
  174. Induction of SAR - Resistant gene induction by salicylic acid (reply: 1)
  175. Seeking House keep gene under 200nt - (reply: 5)
  176. DNA methylation and gene silencing - (reply: 5)
  177. Disease gene clustered on chromosomes - Is there any truth to this? (reply: 1)
  178. How to find a gene's promoter sequence? - (reply: 1)
  179. How to study the expression of one, specific gene? - (reply: 5)
  180. How to get genes from a cytoband - (reply: 1)
  181. GFP as a reporter gene? - (reply: 5)
  182. RT-PCR a GC Rich (68%) gene (2200bp) - (reply: 6)
  183. house keeping gene - (reply: 3)
  184. software for gene designing - (reply: 1)
  185. Cloning of long gene - (reply: 1)
  186. toxic gene ligation/transformation URGENT HELP! - (reply: 1)
  187. What makes a gene a transcription Factor - (reply: 4)
  188. Positive Control for Methylation Studies in BSP sequencing - A "Universally" methylated Gene ?? (reply: 7)
  189. Find promoter sequence of a gene - (reply: 10)
  190. multiple copies of gene or stronger promoter..? - (reply: 2)
  191. Reference Gene - How close is close? - (reply: 4)
  192. No success with PCR cloning of a gene into expression vector - (reply: 5)
  193. Which housekeeping genes for qRT-PCR control? - (reply: 17)
  194. coexpression of different genes in the same host - (reply: 2)
  195. 5' RACE - gene specific primer - (reply: 1)
  196. Low PCR product for antibody gene - (reply: 4)
  197. Help: got the stable cell line without my target gene - (reply: 3)
  198. expressing a gene in a cell HELP - (reply: 1)
  199. DNA methylation analysis of the target gene - (reply: 2)
  200. Cloning gene behind GFP - Vector? (reply: 2)
  201. ask some question about gene expression data - (reply: 1)
  202. Problem with calculation using comparative method - When housekeeping gene is expressed less than sample (reply: 1)
  203. any site for CBF genes of arabidopsis - (reply: 2)
  204. Gene Copy Numbers - (reply: 5)
  205. Housekeeping gene for RT-PCR on plant - RT PCR (reply: 1)
  206. how to determine tissue specific expression of gene? - (reply: 2)
  207. Problem with QPCR - control gene does not amplify - (reply: 2)
  208. Gene3D db ? How can I visualise the nucleotides of a gene ? - (reply: 1)
  209. gene expression - (reply: 2)
  210. Molecular Basis of genes - (reply: 3)
  211. RT-PCR primers for E. coli LacZ gene - (reply: 3)
  212. RNAi in plant and detect with real time PCR - Detect gene expression decrease with qRT-PCR (reply: 2)
  213. How to retrieve a promoter sequences for a given gene ? - (reply: 2)
  214. Taqman for low levels of a gene (LacZ gene)in tissue - (reply: 1)
  215. gene promoter - promoter luciferase (reply: 3)
  216. determine the molecular mechanisms for gene regulation - (reply: 1)
  217. Some Suggestions for Systematic Gene Knockout pls - (reply: 1)
  218. Question about Nox gene - (reply: 1)
  219. gene orientation - (reply: 6)
  220. Help!Checking the nested Primer - P15 gene nested MSP (reply: 2)
  221. Which site of NDV NP gene is cut by BamHI and XhoI? - (reply: 2)
  222. Need Help; regulation of bacterial genes ? - (reply: 3)
  223. different siRNA for the same gene cause opposite effect - (reply: 6)
  224. Gene Expression Data - Looking for gene expression data (reply: 2)
  225. gene expression vs protein expression - (reply: 3)
  226. PCR of AmpR gene - PCR of Amp gene (reply: 1)
  227. Question about imprinted genes - (reply: 3)
  228. Cloned genes - (reply: 1)
  229. Can bacteria "store" gene products? - (reply: 2)
  230. Help!... Stable transfection.. - A293T with puromycin gene.. (reply: 3)
  231. Vector Construction- Tagging N-term - necessity of ATG in Gene of Interest (reply: 2)
  232. suicide gene in bacteria - urgent help needed please. (reply: 3)
  233. Problem with expression of marker gene - (reply: 2)
  234. How to predict a promoter region for a gene - Promoter cloning + genes into vector (reply: 6)
  235. site directed mutagenesis - point mutagenesis of target gene (reply: 3)
  236. How would one go about moving a gene from plasmid to plasmid - cloning (reply: 2)
  237. Housekeeping Gene Selection / RNA purification troubles - Molecular Biology (reply: 2)
  238. Lower Ct for gene of interest than housekeeping gene - (reply: 12)
  239. Gene finder website - (reply: 1)
  240. 5-AZA/TSA upregulation of gene in cell lines - Gene induction (reply: 3)
  241. overlap extension and clone gene into pET -15b - a question?? (reply: 3)
  242. Gene expression comparison between different species(!) - (reply: 1)
  243. How to analyze a gene sequence? - (reply: 2)
  244. Cloning a gene without the sequence of the target species - (reply: 3)
  245. cloning genes with incompatible restriction sites - Protein expression (reply: 2)
  246. how to analyse the gene haplotypes - (reply: 1)
  247. QPCR - QPCR housekeeping gene (reply: 1)
  248. gene deletion via the RED recombinase method - are you experienced? (reply: 3)
  249. moc1 gene - (reply: 2)
  250. Normalizing protein expression under 24hrs ischemia - normalization with housekeeping genes (reply: 5)
  251. Internal control gene -Rt-qPCR - (reply: 1)
  252. PCR off of Genomic DNA problems! - -why is my gene without introns showing up?- (reply: 3)
  253. posible new gene - (reply: 1)
  254. I have a phenotype, but northern says gene is not knocked down - rnai knockdown (reply: 2)
  255. Gene expression in mammalian cells - (reply: 6)
  256. Gene expression - inducible Gene expression (reply: 1)
  257. RT-PCR for an intronless gene - (reply: 6)
  258. How important is cloning of full-length genes? - (reply: 1)
  259. software for drawing gene structure - please help! (reply: 4)
  260. AB resistance gene - Which antibiotic resistance gene in my vector ? (reply: 3)
  261. Get the gene by Geneinfo number - (reply: 2)
  262. Gene promoter - (reply: 2)
  263. Stable cell lines not overexpressing my gene of interest! - Technical problems (reply: 2)
  264. problems with PCR long target gene - (reply: 2)
  265. Transposon mutagenesis of Pseudomonas with Epicentre kit - Need a method for identifying the disrupted gene (reply: 1)
  266. How to find the transcription start site of an unknown gene? - (reply: 1)
  267. Reference gene - (reply: 3)
  268. Clone blunt ended insulin gene into pbr322 - help (reply: 3)
  269. Who is my closest gene relative? - Help to determine phylogeny of a protein (reply: 2)
  270. how to find promoter region of a gene - (reply: 2)
  271. Candidate gene methylation analysis - Some advice needed! (reply: 2)
  272. Method to look for novel methylated gene - (reply: 3)
  273. custom gene synthesis - (reply: 3)
  274. cloning upper stream of a gene - (reply: 1)
  275. How to get the promoter sequence of a gene - (reply: 4)
  276. Promoter Finder - How to find out promoter of known genes (reply: 2)
  277. clone - how to clone an unknown gene (reply: 1)
  278. Housekeeping gene - (reply: 1)
  279. Improve expression level in E. coli - How th improve expression of exogenuous gene in E. coli? (reply: 2)
  280. How can I clone the promoter region of my gene - (reply: 3)
  281. How can I clone the upstream or downstraem of my gene?? - How can I clone the upstream or downstraem of my gene?? (reply: 1)