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Gene Related Discussions
  1. gene expression - expression pattern (reply: 2)
  2. no amplification in target gene - (reply: 1)
  3. eukaryotic gene structure - (reply: 1)
  4. Compare the expression of two target genes - (reply: 3)
  5. How to isolate intron of particular gene - (reply: 3)
  6. gene cloning questions - (reply: 3)
  7. Baseline and threshold values for a reference and target gene can be different? - (reply: 2)
  8. need an example of a gene with a drug target.... - (reply: 1)
  9. a gene instead an other.. - (reply: 1)
  10. House keeping gene copy number - (reply: 3)
  11. knocking down endogeous gene using shRNA - (reply: 6)
  12. expression level comparison - a database to find gene expressi (reply: 2)
  13. Gene Expression level of apoptotic-related genes by qReal Time RT PCR - (reply: 1)
  14. "Gene expression vs Transcription vs mRNA levels": Help with conceptua - (reply: 1)
  15. mRNA levels, transcription or Gene expression? - (reply: 2)
  16. gene expresion - (reply: 1)
  17. siRNA designation for different genes - (reply: 1)
  18. tagging of gene with GUS gene - (reply: 1)
  19. Why is antagonist inducing the gene? - (reply: 1)
  20. Gene complexity and protein complexity analysis - (reply: 2)
  21. primer design software - primes for full lenth gene (reply: 3)
  22. DeltaDelta Ct Relative or Comparative Quantitation - methods of relative quantitation for qPCR determining gene expression (reply: 3)
  23. Kanamycin resistance gene in Gram-positive bacteria - (reply: 2)
  24. Why DNA sequencing does not detect deletions - help! - Med student lost on her hw. Breast cancer gene (BRCA1) mutations (reply: 3)
  25. Study of gene family evolution - (reply: 2)
  26. can shRNA expression set share one polyA terminator with other gene - (reply: 3)
  27. Large variation in replicates in samples with low gene copy by methyLight. - (reply: 5)
  28. Gene onthology software - (reply: 1)
  29. gene variants - on same or different chromosomes (reply: 1)
  30. Tool to map genes on chromosomes - (reply: 3)
  31. Help on How to find sequence of certain gene - (reply: 1)
  32. mRNA Expression - How to observe mRNA expression of gene. (reply: 10)
  33. Problems with Mutations of 2.1 kb Gene - (reply: 7)
  34. Looking for nucleotide sequence of rearranged antibody gene locus. - (reply: 1)
  35. how to optimize rt-pcr for gene expression analysis - (reply: 3)
  36. Gene Knockout - (reply: 2)
  37. Do we have fluorescence genes? - genes which code for fluoscent proteins (reply: 12)
  38. Best chemistry for relative quantitation of gene expression - Give your opinion on the best chemistry according to your experience (reply: 5)
  39. transfecting large gene in mammalian cells - difficulties in transfecting large gene in mammalian cells (reply: 5)
  40. gene expression analysis - (reply: 1)
  41. Are all growth stimulatory genes proto-oncogenes? - (reply: 1)
  42. housekeeping genes for brain tissue - (reply: 4)
  43. housekeeping genes for brain tissue - (reply: 3)
  44. gene dowregulation by miRNA - (reply: 1)
  45. Housekeeping genes validation - (reply: 3)
  46. house keeping gene not intron spanning and sample DNase UNtreated - (reply: 1)
  47. housekeeping gene in klebsiella pneumoniae for RT PCR - (reply: 2)
  48. B-catenin pathway - Lef/TCF-sensitive reporter gene assay (reply: 4)
  49. how to select a calibrator for relative gene expression in real-time PCR - also about the inconsistant about the triplicate of real-time PCR (reply: 6)
  50. Help: JIGSAW- gene prediction - (reply: 1)
  51. 4 subunit gene: How to clone? - (reply: 1)
  52. Bulk extraction of genes information - (reply: 1)
  53. siRNA in organotypic cultures - siRNA-mediated gene silencing in vitro and in vivo (reply: 6)
  54. Identification of weakly expressed novel gene - (reply: 1)
  55. identify cis-elements in co-regulated genes - (reply: 1)
  56. Bacterial gene disruption problem - (reply: 2)
  57. gene domains - (reply: 1)
  58. Can we measure gene expression with standard PCR? - (reply: 1)
  59. How do I make a graph displaying "# of Genes" vs. "Expression Lev - (reply: 4)
  60. Can i use old standard curve to determine gene concentration? - (reply: 1)
  61. Best house keeping gene for RT-PCR - (reply: 6)
  62. Cutting out EGFP gene from pEGFP-N1 - w/o using XbaI (reply: 2)
  63. cutting out EGFP gene from pEGFP-N1 vector - what does dam- mean? (reply: 1)
  64. qPCR - running taqman for endogenous control and SYBR for the gene - (reply: 2)
  65. Housekeeping gene validation - (reply: 7)
  66. housekeeping gene for B cell lines - (reply: 1)
  67. :ph34r: I am lost - Gene Hunting Guide , please - (reply: 2)
  68. incorporation of restriction site in Primers - Help to design primer to clone gene in frame with fluorescent tag (reply: 2)
  69. No mRNA or protein made by my mutant gene - (reply: 4)
  70. lux gene cloning using Sal I as restriction enzyme - (reply: 1)
  71. Cloning directionality and terminator availability - is lac z gene terminator is enough for my gene termination?? (reply: 1)
  72. how to check the insertion of a gene fragment into plasmid - (reply: 2)
  73. Cancer Genes Data Base - (reply: 1)
  74. I want to find the promoter sequence of human IL-1 beta gene - (reply: 1)
  75. TaqMan probes specificity - I need to amplify and quantify specifically homologous genes (reply: 4)
  76. gene copy number - how to find how many? (reply: 12)
  77. How to convert coordinates into genes - (reply: 3)
  78. Analyzing microarray data: a tool for annotations and grouping genes? - (reply: 2)
  79. serching for genes - (reply: 1)
  80. Interpreting PCA Graph for Gene Expression - Principle Component Analysis in Gene Expression (reply: 1)
  81. Cloning 1.5kB gene into lentivirus--I'm stumped - (reply: 2)
  82. RNAi of two different genes - (reply: 4)
  83. Is it total ORF is required for complete gene expression - (reply: 3)
  84. Finding correlated genes between microarray data sets - (reply: 3)
  85. How to find DNA sequence upstream of gene? - (reply: 3)
  86. which part of gene is needed for normal expression in vector - (reply: 2)
  87. how to isolate gene through PCR - (reply: 3)
  88. How to target a gene for deletion - (reply: 2)
  89. Combinatorial RNAi - double gene knockdown - (reply: 4)
  90. how to check organ(tissue)-specific expression profile of a gene - (reply: 3)
  91. Disease causing genes - (reply: 2)
  92. housekeeping gene for comparing gene expression between tumor and normal tissue - (reply: 1)
  93. how to clone the promoter of a gene - (reply: 3)
  94. How to identify novel gene using bioinfomatics - (reply: 3)
  95. generating an array by gene amplification - (reply: 2)
  96. PCR on this gene - products always present? - (reply: 9)
  97. How long does the methylation status of a gene last after AZA treatment? - (reply: 2)
  98. Gene Deletion in E.Coli Bl21 - (reply: 8)
  99. gene down-regulated in cancer cell line but no proliferation inhibit ability - (reply: 3)
  100. what are 'floxed' genes in interbred mouse - (reply: 1)
  101. has anyone here ever amplified chloroplast matK gene before? - (reply: 2)
  102. Does kanR gene confer resistance to Ampicillin? - (reply: 1)
  103. Quantitative PCR question - Comparative quant PCR with 16S rRna as reference gene (reply: 2)
  104. Siginificant Homology at the Amino acid level? - Gene sequence homology (reply: 1)
  105. Question about interpretation of Northern Blots - apparent incomplete transcription of genes in bacteria (reply: 3)
  106. Techanical Question about cloning of gene - (reply: 2)
  107. Cloning of genes - (reply: 2)
  108. Ampiciline resistance gene - (reply: 1)
  109. Taqman target gene and sybr green endogenous control? - (reply: 1)
  110. Amplifying large gene - (reply: 2)
  111. Housekeeping gene not affected by HGF treatment - (reply: 2)
  112. NCBI gene sequence SOURCE? - (reply: 7)
  113. animal model of gene expressed in one embryonic layer - (reply: 1)
  114. confirming how the gene causes decreased growth/increased cell death - (reply: 4)
  115. RNA extraction from laser capture microdissections - how to see low expressed genes from trought RT/qPCR on laser capture m (reply: 2)
  116. Confirmation of protein expression in pCDNA3 - How to know foreign gene expression in pCDNA3 (reply: 8)
  117. Finding genes in a region - have two snps, need genes in between (reply: 1)
  118. Real time pcr in Rotor gene 6000- not getting positive results - (reply: 1)
  119. blasting gene of interest - (reply: 1)
  120. gene duplication - affected by siRNA? (reply: 1)
  121. design primers with custom end - To clone my gene into the MCS as fusions to the GFP tag (reply: 5)
  122. Housekeeping genes for rat brain - (reply: 4)
  123. How to search plasmid sequence for restriction sites and resistance genes? - (reply: 4)
  124. Chromosomal Gene Deletion - Gene Deletion by Double Crossing Over in Pseudomonas (reply: 6)
  125. Housekeeping genes for RT-PCR in soybean - (reply: 6)
  126. Real-Time PCR and Housekeeping gene - (reply: 9)
  127. Expression vector for Pseudomonas - expression of a 10Kb gene in Pseudomonas aeruginosa (reply: 2)
  128. gene from 4-TOPO to expression vector - (reply: 1)
  129. Cloned genes dont express! - (reply: 6)
  130. help!real-time PCR to see gene's expression after treatment with cytokin - (reply: 4)
  131. GAPDH primer sequence - cloning of huGAPDH gene (reply: 2)
  132. New gene insertion in yeast - What are the suitable loci? (reply: 3)
  133. Finding Gene Sequences - (reply: 4)
  134. gene prediction tools - (reply: 1)
  135. How to find a gene in a genome that has a high frequency of specific amino acid? - (reply: 1)
  136. histone modification-ChIP analysis - selection of proper internal control for repressed state of gene (reply: 2)
  137. Isolation entire gene from degenerate primer - isolation of entire gene (reply: 1)
  138. Is this gene going to express? - is only the firs ATG read and express or all 3 reading frame? (reply: 10)
  139. Questions about gene expression, codons, and Kozak sequence - (reply: 4)
  140. gene hunting advice - (reply: 2)
  141. weird problem with Primers! - problem with the gene sequence i used to design my primers (reply: 4)
  142. Genorm - Validation of housekeeping genes - (reply: 1)
  143. fibroblast marker genes - fibroblast marker genes (reply: 1)
  144. Can you get two proteins made from a heterozygous gene mutation? - (reply: 3)
  145. imprinted genes - which parent? - (reply: 1)
  146. Affymetrix chip - unexpressed genes, which signal cuttof to use - (reply: 1)
  147. How to overexpress a gene in C.elegans - (reply: 3)
  148. growing after filling in monolayer, please help - How and which genes/growth factors do it (reply: 5)
  149. how to find p53 bibding site in my target gene promoter - (reply: 4)
  150. gene was amplified when -RT were used as template? - (reply: 4)
  151. cloning vector for high GC ratio gene? - is GC ratio a criteria in choosing vector?? (reply: 2)
  152. Cloning 2kb+ gene by PCR - Is it doable? (reply: 3)
  153. GC plot - GC content of a gene in a plot? (reply: 2)
  154. How to find miRNA precursor (or gene) for a specific miRNA - (reply: 2)
  155. PCR - 16S Gene (reply: 3)
  156. primers dont match FASTA gene sequence... 'm confused - (reply: 11)
  157. How to know tissue specific gene expression by real time PCR - (reply: 3)
  158. BSP to test imprinted genes DNA methylation of oocytes - no specific or smear bands! (reply: 4)
  159. Methods needed for gene/protein identification and function - (reply: 2)
  160. How to amplify 4.1Kb gene from genomic DNA? - (reply: 15)
  161. How to select a cell type to study my gene - (reply: 2)
  162. How to find promoter region of rat gene? - (reply: 2)
  163. miRNA gene knockdown? - (reply: 1)
  164. comparing difference in gene expression - (reply: 2)
  165. Gene without stop codon - (reply: 4)
  166. software for CpG island prediction for a list of genes - (reply: 3)
  167. isolate genes affecting K+ export in Sac.cerv. - (reply: 3)
  168. quantifying my gene expression in 5 different cell lines - (reply: 1)
  169. What exactly does it mean by cloning a toxic gene - (reply: 2)
  170. Does a gene have an associated CpG island? - (reply: 9)
  171. Drosophila: Gene knockout using a transposon technology - Why do you get a gene expression? Other methods to knockout a gene? (reply: 7)
  172. Finding a target gene sequence in rat genome using a mice gene - How to find the delta opioid receptor gene in rat if the sequence is k (reply: 2)
  173. route map for cloning a gene - steps involved in subcloning a gene (reply: 3)
  174. Cloning toxic gene - (reply: 2)
  175. gene targeting in ES cells - (reply: 1)
  176. Scramble siRNA shows increase in gene expression by Taqman RTPCR - (reply: 1)
  177. What about the contamination in negative control with higher Tm than target gene - (reply: 1)
  178. Gene expression - (reply: 1)
  179. urgent help-how to get amplification from very low target copy number and how to - "Taqman probe" qPCR and "rotor gene 6000" machine (reply: 4)
  180. how to compare promoter regions from two genes - (reply: 2)
  181. About how to find out the gene(s) - (reply: 6)
  182. neomycin-resistant gene vs kanamycin - (reply: 3)
  183. Imprinted gene control in breast tissue? - (reply: 1)
  184. porcine CD4 gene sequence needed - urgently - (reply: 2)
  185. Search for gene sequence? - (reply: 3)
  186. how to switch to a gene in a vector using PCR or restriction enzymes? - gene is in pIVS2 vector (reply: 3)
  187. how to ligate a gene repeatedly - (reply: 6)
  188. Advice on analysing Differentially Methylated Regions of a gene. - (reply: 5)
  189. Can I use different amount of cDNA template to run real time PCR? - when the amount of templates are different,Can I use reference gene to (reply: 2)
  190. Genes/Proteins - (reply: 6)
  191. find promoter sequences - find promoter sequences of the pgk2 gene in different species like opp (reply: 1)
  192. how to compare 2 different gene expression level in the same sample? - can I use ratio(A/B)=2^(CtB-CtA)to calculate these two genes' expr (reply: 5)
  193. Gene Expression Omnibus - (reply: 2)
  194. How to find promoter region between two closely placed genes - (reply: 1)
  195. RT-QPCR of cells treated with 5-aza - which housekeeping gene to use, different cell lines (reply: 1)
  196. gene disruption - should regions flanking a cassette be of equal size (reply: 2)
  197. reporter gene -seap - expression of seap is very low (reply: 24)
  198. Induce mutation in my gene - (reply: 5)
  199. IRES sequence. Different gene's expression. - (reply: 2)
  200. reporter gene fusion - (reply: 3)
  201. Linkage analysis and how to locate genes? - (reply: 1)
  202. Bacterial housekeeping genes - What genes are commonly used as housekeeping controls in expression st (reply: 1)
  203. How isolate a 4kbp gene?! - (reply: 3)
  204. Reference Gene: human leukocytes - (reply: 2)
  205. Kozak sequence between GST and gene of my interest - There is a kozak sequence between GST and my gene. Does it hurt? (reply: 1)
  206. rrn gene copy numbers - (reply: 1)
  207. Eclone = ? for In Situ and mRNA injection - What is the official gene name for "eclone" ? (reply: 1)
  208. Maximum acceptable variation of Housekeeping gene - (reply: 1)
  209. problem rt pcr long gene - (reply: 4)
  210. detection of mec A gene - (reply: 1)
  211. how to construct the gene of a functional domain? - (reply: 2)
  212. pcr for gene fusion - (reply: 3)
  213. how to test the genetic contribution of modifier gene to the disease in multiple - (reply: 1)
  214. Imprinted genes as a control? - (reply: 1)
  215. Protocol for amplification of gene in plasmid directly from transformed e. coli? - (reply: 2)
  216. mutation in a gene that is not expressing a protein - (reply: 4)
  217. Why few hubs control gene regulation? - (reply: 1)
  218. Long arm short arm of a gene/RF, not chromosome - (reply: 1)
  219. Are we all born with the same genes? - (reply: 1)
  220. impossible gene ! - (reply: 3)
  221. which killer gene - (reply: 11)
  222. integration vector question - do i need to clone a full gene (reply: 1)
  223. Stable transfection using plasmid with no selection gene - (reply: 2)
  224. About housekeeping gene - (reply: 3)
  225. RT-PCR gene specific amplification problem - (reply: 1)
  226. housekeeping genes - (reply: 1)
  227. whole gene synthesis for expression - (reply: 3)
  228. Database of embryonic expressed genes - (reply: 2)
  229. multiple identical genes in an organism - (reply: 3)
  230. problem with -ve controls of mt genes - (reply: 1)
  231. Traget gene has the same Ct as HK gene - what to do ? - HELP !!!!!!!!! (reply: 4)
  232. Need a weak promoter for reporter gene assay - (reply: 3)
  233. Cell count vs Reference gene - Cell count vs Reference gene (reply: 1)
  234. Cloning incomplete genes - (reply: 2)
  235. CHIP assay shows same fold enrichments over IgG in both my gene promoter and bet - CHIP negative control (reply: 2)
  236. How to add a lethal gene to my plasmid for cloning selection? - (reply: 3)
  237. low to no gene expression - (reply: 4)
  238. transcription of the genes in one operon - (reply: 1)
  239. Location of a gene in several chromosomes_Is that possible? - (reply: 4)
  240. ways to get a good gene amplification - (reply: 4)
  241. Can shRNA be used in one transcript with other gene - (reply: 19)
  242. can cells just stop having the gene? - (reply: 4)
  243. How do you find all the genes regulated by a particular transcription factor? - (reply: 1)
  244. how to amplify a long gene? - (reply: 3)
  245. Tips on isolating low abundance genes using PCR - any advice? (reply: 3)
  246. Quorum sensing gene cloning - (reply: 7)
  247. Isolation of the upstream region of a gene by PCR: a new tecnique? - (reply: 4)
  248. Gene body methylation and active gene expression - (reply: 6)
  249. Why the DNA sequence big different but protein the similarity? - such as MAOA and MAOB genes (reply: 4)
  250. Replacing small gene with large gene - (reply: 2)
  251. gene transfer via agrobacterium - how to prepare bacteria??? (reply: 7)
  252. Strategies for gene cloning and expression - Discuss various methods for it (reply: 8)
  253. my vector contain ccdB gene in MCS, can i use this as mock control during transf - (reply: 1)
  254. two additional amino acids in gene - (reply: 4)
  255. developmental genes screening - (reply: 2)
  256. Excision of foreign genes by E.coli - suspicion of attack (reply: 5)
  257. Primer Design for Gene Inserted Counterclockwise into Plasmid - Please help...very basic question for a mol. biologist (reply: 1)
  258. does size matter - how many genes in 1 vector (reply: 10)
  259. Ct for housekeeping gene is higher than Ct of target gene. - (reply: 1)
  260. RNAi targeting one gene in a bicistronic transcript - Will the other gene be knock down? (reply: 4)
  261. Mouse -> Human Gene homologue finder/conversion tool - (reply: 1)
  262. RFLP versus a single gene to study genotypic diversity - (reply: 4)
  263. How to batch download human genomic sequences for genes? - (reply: 3)
  264. how to find the gene promoter region? - (reply: 1)
  265. gene expression study - between treated and untreated cells (reply: 2)
  266. how to get specific amplfication of a gene that is extremely similar to another - (reply: 7)
  267. gene delivery lipofectamine - lipoplexes acetone (reply: 4)
  268. How long does it take for the gene to lose? - (reply: 2)
  269. How to determine 3' end sequence of bacterial gene - (reply: 6)
  270. cDNA for real time PCR - for gene expression study (reply: 1)
  271. HELP with DESIGNING GENETIC EXPT. - To determine function of TOPLESS gene/function (reply: 2)
  272. Creating Multimer Genes, cloning - cloning (reply: 1)
  273. linear tandem repeats of gene for transient transfection? - (reply: 3)
  274. Cloning of gene from peptide sequence - (reply: 1)
  275. which expert have cloned grp78 gene - (reply: 3)
  276. transfer gene to protein? - (reply: 3)
  277. Validating amplification efficiencies of the target and the housekeeping genes - (reply: 1)
  278. Used linear concatameric genes for transient transfection? - (reply: 2)
  279. Gene sequencing problem - Getting a non gene of interest (reply: 1)
  280. how to know/predict a specific gene is regulated by miRNA - (reply: 7)
  281. gene/ORF - (reply: 11)
  282. PCR'ing a high GC gene - (reply: 1)
  283. Real Time PCR : Bacterial gene quantification, can it be done? - (reply: 2)
  284. which is the best approach for simultaneous siRNA of two genes? - (reply: 4)
  285. Sparks while electroporating via gene pulser - Inquiry (reply: 9)
  286. SDS page and gene cloning - pBS plasmid (reply: 1)
  287. housekeeping gene for lymphocyte - (reply: 1)
  288. subcloning dengue NS1 gene problems - molecular biology, cloning (reply: 5)
  289. Can I PCR a gene of 2KB? - (reply: 3)
  290. Is it absolutely necessary to do BGS before doing MSP for new genes? - (reply: 4)
  291. are there such software that can cluster genes by their function? - (reply: 2)
  292. GAPDH endogenous control gene - (reply: 2)
  293. Gene cloning - Gene library (reply: 1)
  294. Real Time PCR and Gene expression - (reply: 1)
  295. Need Some Math Help - stimulation of gene expression (reply: 3)
  296. Inserting promoters upstrem of reporter genes - (reply: 2)
  297. Amount of E. coli rRNA gene copy - (reply: 3)
  298. where can I get any plasmid with aadA as selective marker gene? - i might offer some money (reply: 1)
  299. Important genes in the Colon cancer microarray data - (reply: 2)
  300. gene of interest or housekeeping gene shift...? - (reply: 3)
  301. Testing expression of OVA gene - (reply: 1)
  302. Why is it difficult to PCR taget gene in rice successfully ? - (reply: 1)
  303. gene annotation? - (reply: 3)
  304. Express miRNA in vector for gene knockdown - (reply: 4)
  305. help in cloning the promoter region of a gene - (reply: 3)
  306. Relative expression of genes between bacterial species - (reply: 1)
  307. clustering: how to - Help on perl scripting for apply clustering to gene caracteristics (reply: 4)
  308. luciferase reporter gene assay - (reply: 3)
  309. How many copies of genes are there in human chormosomes? - (reply: 7)
  310. RT-PCR of apoptosis related genes - (reply: 2)
  311. inserting shRNA vs full gene into vector ? - (reply: 10)
  312. Is all lab bacterial strains have t7 RNA polymerase gene... - (reply: 4)
  313. Problems with inverse PCR - Identification of gene trap vector insertion (reply: 3)
  314. amount of DNA for low copy number gene - (reply: 1)
  315. Gene expression on same Chromosome region possible? - microarray (reply: 3)
  316. bacteria gene knockout using Red recombination system - (reply: 1)
  317. what is a suppressor gene? - basic knowledge (reply: 3)
  318. Reference genes on same plate as gene of interest? - (reply: 2)
  319. how to rigorously demonstrate the one gene is up-regulated in the transcriptiona - (reply: 6)
  320. PCR clone a gene with known 3' end only - (reply: 4)
  321. reporter genes - (reply: 1)
  322. where to find the list of genes known to be methylated - (reply: 2)
  323. help on yeast gene knock-out - (reply: 2)
  324. semi-qRT-PCR - problem with housekeeping gene (reply: 1)
  325. Searching for gene ontology terms automatically and adding to a text file - (reply: 20)
  326. change in expression of House keeping gene - change in expression of House keeping gene (reply: 2)
  327. Pyrosequencing imprinted genes - (Am i thinking straight?) :( (reply: 8)
  328. primer dimer in pcr product - cloning for a gene. analyzing pcr product shows primer dimer (reply: 8)
  329. Must I do MSP focused on survivin gene's exon 1 . - (reply: 2)
  330. Replacing of GFP cloned inside a gene with RFP - (reply: 2)
  331. Housekeeping gene always on the same plate as samples? - (reply: 1)
  332. Problem PCR amplifying gene promoter regions - (reply: 4)
  333. Sequence error in cloned expressed gene - how to correct it? - (reply: 15)
  334. official database for cancer-related genes - (reply: 3)
  335. housekeeping gene in real-time PCR - (reply: 2)
  336. how the gene cloned into a vector can expressed in mammalian cell without the 5& - What is the mechanism of translation initiaition with the HCM promoter? (reply: 4)
  337. Finding promoter sequence of a gene - (reply: 2)
  338. 3'UTR and Gene expression in expression construct - (reply: 2)
  339. Tetracycline System for E. coli and mammalian - Mammalian Gene Regulation (reply: 2)
  340. qPCR with Corbett Rotor Gene 6000 - (reply: 3)
  341. Show Gene pathway - a computative program - (reply: 2)
  342. PCR analysis of clones - Can a gene specific primer pair amplify a fragment in the wrong direction? (reply: 1)
  343. Amplification of gene (CHH,MIH,GIH) from prawns. - Amplification of gene using primers designed on the basis of cDNA sequences (reply: 1)
  344. induction estrogen responsive gene (PS2 & PgR) in MCF7 - (reply: 3)
  345. gene expression assay - (reply: 1)
  346. co-transfection - co-transfection affect gene expression? (reply: 4)
  347. HK gene overabundance - (reply: 3)
  348. Induction of SAR - Resistant gene induction by salicylic acid (reply: 1)
  349. Gene synthesis - (reply: 1)
  350. Seeking House keep gene under 200nt - (reply: 5)
  351. Gene synthesising - (reply: 1)
  352. DNA methylation and gene silencing - (reply: 5)
  353. Screening a Gene Library - I want to use colony PCR, but How (reply: 4)
  354. Disease gene clustered on chromosomes - Is there any truth to this? (reply: 1)
  355. Real time PCR for gene family - (reply: 3)
  356. How to find a gene's promoter sequence? - (reply: 1)
  357. How to find the promoter sequence of this gene - (reply: 14)
  358. How to study the expression of one, specific gene? - (reply: 5)
  359. Want to find a database to get gene annotaions by inputting cytoband as a query - (reply: 2)
  360. How to get genes from a cytoband - (reply: 1)
  361. DNA methylation vs gene expression - (reply: 3)
  362. GFP as a reporter gene? - (reply: 5)
  363. length of reporter gene - (reply: 6)
  364. RT-PCR a GC Rich (68%) gene (2200bp) - (reply: 6)
  365. Efficiency differences between target and housekeeping gene - (reply: 1)
  366. house keeping gene - (reply: 3)
  367. How to add a sequence "inframe" of my gene? Help please! - (reply: 4)
  368. software for gene designing - (reply: 1)
  369. What to choose as a calibrator? - When looking at basal gene expression (reply: 7)
  370. Cloning of long gene - (reply: 1)
  371. transposon methylation and gene silencing - (reply: 9)
  372. toxic gene ligation/transformation URGENT HELP! - (reply: 1)
  373. Effect of scrambled siRNA on gene expression - (reply: 5)
  374. What makes a gene a transcription Factor - (reply: 4)
  375. How many genes in Arabidopsis thaliana ? - (reply: 2)
  376. Positive Control for Methylation Studies in BSP sequencing - A "Universally" methylated Gene ?? (reply: 7)
  377. Imprinted gene AND DMR - (reply: 9)
  378. Find promoter sequence of a gene - (reply: 10)
  379. Anti-Puromycin Resistance Gene Antibody - Anyone know of one? (reply: 3)
  380. multiple copies of gene or stronger promoter..? - (reply: 2)
  381. website for identifying restriction sites in a gene - (reply: 6)
  382. Reference Gene - How close is close? - (reply: 4)
  383. gene extraction - (reply: 7)
  384. No success with PCR cloning of a gene into expression vector - (reply: 5)
  385. house keeping gene in skeletal muscle - (reply: 2)
  386. Which housekeeping genes for qRT-PCR control? - (reply: 17)
  387. Trying on genes - (reply: 1)
  388. coexpression of different genes in the same host - (reply: 2)
  389. two bands of one gene in PCR - (reply: 8)
  390. 5' RACE - gene specific primer - (reply: 1)
  391. express bacteria gene in planta - (reply: 1)
  392. Low PCR product for antibody gene - (reply: 4)
  393. epistasis, epistatic genes - (reply: 8)
  394. Help: got the stable cell line without my target gene - (reply: 3)
  395. Splitting transfected cells: do they still overexpress my gene of interest ? - (reply: 5)
  396. expressing a gene in a cell HELP - (reply: 1)
  397. design spesific primer of avr, hrp, toxin and falgella gene - (reply: 3)
  398. DNA methylation analysis of the target gene - (reply: 2)
  399. unstable result with report gene analysis - (reply: 1)
  400. Cloning gene behind GFP - Vector? (reply: 2)
  401. linking gene to disease - (reply: 2)
  402. ask some question about gene expression data - (reply: 1)
  403. Multicopy Gene - (reply: 2)
  404. Problem with calculation using comparative method - When housekeeping gene is expressed less than sample (reply: 1)
  405. Rice Gene Nomenclature - Need help here, please (reply: 2)
  406. any site for CBF genes of arabidopsis - (reply: 2)
  407. IPTG expression w/ GST-fusion gene - (reply: 5)
  408. Gene Copy Numbers - (reply: 5)
  409. Technology for gene knockout at transcriptional level - (reply: 3)
  410. Housekeeping gene for RT-PCR on plant - RT PCR (reply: 1)
  411. especial cell line and gene, what is the result of RNA? - select a more inexpensive siRNA (reply: 2)
  412. how to determine tissue specific expression of gene? - (reply: 2)
  413. How to normalize with 2 housekeeping genes? - (reply: 4)
  414. Problem with QPCR - control gene does not amplify - (reply: 2)
  415. Western Blot Hela Cos7 - Show nearly identical genes of two related organisms same expression (reply: 4)
  416. Gene3D db ? How can I visualise the nucleotides of a gene ? - (reply: 1)
  417. Amplification of specific genes from cDNA - (reply: 3)
  418. gene expression - (reply: 2)
  419. How to choose siRNA when BLAST results show high similarity with other gene - (reply: 5)
  420. Molecular Basis of genes - (reply: 3)
  421. Down-regulation of house-keeping genes in colon tissue of DSS-fed mice - (reply: 1)
  422. RT-PCR primers for E. coli LacZ gene - (reply: 3)
  423. Primer for 4 genes sharing short consensus sequence - (reply: 2)
  424. RNAi in plant and detect with real time PCR - Detect gene expression decrease with qRT-PCR (reply: 2)
  425. Basic fundamental principles of gene knockout - (reply: 2)
  426. How to retrieve a promoter sequences for a given gene ? - (reply: 2)
  427. efficiency problem - HKG's lower than target genes - (reply: 12)
  428. Taqman for low levels of a gene (LacZ gene)in tissue - (reply: 1)
  429. Methylation and expression of imprinted genes - (reply: 3)
  430. gene promoter - promoter luciferase (reply: 3)
  431. how to mutant the gene on the genomic DNA? - (reply: 9)
  432. determine the molecular mechanisms for gene regulation - (reply: 1)
  433. general question- how to study regulation of gene expression - (reply: 2)
  434. Some Suggestions for Systematic Gene Knockout pls - (reply: 1)
  435. Amplification of the uncharacterized regions by Inverse PCR - what's the application since the target gene is inverted after it? (reply: 2)
  436. Question about Nox gene - (reply: 1)
  437. Single copy gene in CHO - (reply: 1)
  438. gene orientation - (reply: 6)
  439. rotor gene software - downloading (reply: 2)
  440. Help!Checking the nested Primer - P15 gene nested MSP (reply: 2)
  441. gene clone - (reply: 1)
  442. Which site of NDV NP gene is cut by BamHI and XhoI? - (reply: 2)
  443. bicistronic retroviral vector - Two both gene mammalian expression (reply: 2)
  444. Need Help; regulation of bacterial genes ? - (reply: 3)
  445. Detection of Bt gene in Corn Through PCR - High School Science Fair - (reply: 6)
  446. different siRNA for the same gene cause opposite effect - (reply: 6)
  447. nutrients & gene expression - (reply: 2)
  448. Gene Expression Data - Looking for gene expression data (reply: 2)
  449. change between neomycin and blasticidin resistance gene - (reply: 10)
  450. gene expression vs protein expression - (reply: 3)
  451. Gene hunt in fantastic microorganism - (reply: 1)
  452. PCR of AmpR gene - PCR of Amp gene (reply: 1)
  453. Quantitative gene knock down with siRNA - (reply: 1)
  454. Question about imprinted genes - (reply: 3)
  455. detection of low expressed genes - (reply: 1)
  456. Cloned genes - (reply: 1)
  457. affymetrix gene chip compering to agilent gene chip - (reply: 2)
  458. Can bacteria "store" gene products? - (reply: 2)
  459. Mouse ES cell culture - ES cell culture for the purpose of gene targeting (reply: 3)
  460. Help!... Stable transfection.. - A293T with puromycin gene.. (reply: 3)
  461. Whole gene cloning - (reply: 2)
  462. Vector Construction- Tagging N-term - necessity of ATG in Gene of Interest (reply: 2)
  463. how to monitor gene expression over time in culture - GFP method (reply: 1)
  464. suicide gene in bacteria - urgent help needed please. (reply: 3)
  465. Problem with expression of marker gene - (reply: 1)
  466. Problem with expression of marker gene - (reply: 2)
  467. one gene, 2 variants - which one is relevant? (reply: 2)
  468. How to predict a promoter region for a gene - Promoter cloning + genes into vector (reply: 6)
  469. true methylation target - downstream of a true methylated gene (reply: 2)
  470. site directed mutagenesis - point mutagenesis of target gene (reply: 3)
  471. NF-kB in transient reporter gene assay - (reply: 1)
  472. How would one go about moving a gene from plasmid to plasmid - cloning (reply: 2)
  473. YFG - Your Favourite Gene? - (reply: 5)
  474. Housekeeping Gene Selection / RNA purification troubles - Molecular Biology (reply: 2)
  475. Genes with no CpG islands... - (reply: 6)
  476. Lower Ct for gene of interest than housekeeping gene - (reply: 12)
  477. workflows - arabidopsis/flanking genes (reply: 1)
  478. Gene finder website - (reply: 1)
  479. How to selecte gene for epigenetics research? - imprinted gene or gene with expression change ? (reply: 1)
  480. 5-AZA/TSA upregulation of gene in cell lines - Gene induction (reply: 3)
  481. Interesting gene expression in a stable transfection - (reply: 2)
  482. overlap extension and clone gene into pET -15b - a question?? (reply: 3)
  483. Issues of EST in whole genome gene discovery - Issues of EST in whole genome gene discovery (reply: 3)
  484. Gene expression comparison between different species(!) - (reply: 1)
  485. Methylation of housekeeping genes? - (reply: 3)
  486. How to analyze a gene sequence? - (reply: 2)
  487. Why a bacterial cell restriction enzyme not cleave foreign gene - (reply: 8)
  488. Cloning a gene without the sequence of the target species - (reply: 3)
  489. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (reply: 5)
  490. cloning genes with incompatible restriction sites - Protein expression (reply: 2)
  491. genes orientation - (reply: 3)
  492. how to analyse the gene haplotypes - (reply: 1)
  493. gDNA, mRNA and cDNA sequences of a gene? - (reply: 5)
  494. QPCR - QPCR housekeeping gene (reply: 1)
  495. calculate copy number of a gene - (reply: 6)
  496. gene deletion via the RED recombinase method - are you experienced? (reply: 3)
  497. Transformation of an unstable gene - (reply: 1)
  498. moc1 gene - (reply: 2)
  499. Same exon-intron junction sequences for all genes? - (reply: 4)
  500. Normalizing protein expression under 24hrs ischemia - normalization with housekeeping genes (reply: 5)
  501. DNMTs repress unmethylated gene? - (reply: 2)
  502. Internal control gene -Rt-qPCR - (reply: 1)
  503. Plant gene expression in bacteria and yeast - (reply: 3)
  504. PCR off of Genomic DNA problems! - -why is my gene without introns showing up?- (reply: 3)
  505. semi quantitative PCR - quantification of gene expression (reply: 1)
  506. posible new gene - (reply: 1)
  507. TSG of NPC (Tumor suppressor genes of nasopharyngeal carcinoma) - (reply: 2)
  508. I have a phenotype, but northern says gene is not knocked down - rnai knockdown (reply: 2)
  509. Poly-A sequences lay upstream of a reporter gene? - (reply: 6)
  510. Gene expression in mammalian cells - (reply: 6)
  511. Primer design for unknown gene - (reply: 4)
  512. Gene expression - inducible Gene expression (reply: 1)
  513. Question about cloning a gene coding an enzyme - (reply: 1)
  514. RT-PCR for an intronless gene - (reply: 6)
  515. Endogengous Versus Overexpressed Target Gene - Knockdown of overexpressed but not endogenous genes...Help! (reply: 7)
  516. How important is cloning of full-length genes? - (reply: 1)
  517. The definition of a real gene? - (reply: 3)
  518. software for drawing gene structure - please help! (reply: 4)
  519. gene linearization for transfection - stable mammalian tranfection. (reply: 1)
  520. AB resistance gene - Which antibiotic resistance gene in my vector ? (reply: 3)
  521. Gene copy number determination - Gene copy number determination (reply: 1)
  522. Get the gene by Geneinfo number - (reply: 2)
  523. About identify the inserted gene? - a using PCRII- topo vector question (reply: 4)
  524. Gene promoter - (reply: 2)
  525. Asking:the standard of a "new" gene - the standard of a "new" gene (reply: 1)
  526. Stable cell lines not overexpressing my gene of interest! - Technical problems (reply: 2)
  527. Diag representation of gene, SNPs - Diag rep of SNPs in gene (reply: 3)
  528. problems with PCR long target gene - (reply: 2)
  529. PCR comparison of gene expression - (reply: 5)
  530. Transposon mutagenesis of Pseudomonas with Epicentre kit - Need a method for identifying the disrupted gene (reply: 1)
  531. Gene Position in Human Genome - (reply: 2)
  532. How to find the transcription start site of an unknown gene? - (reply: 1)
  533. stable clones - but expressing the gene! help! - (reply: 2)
  534. Reference gene - (reply: 3)
  535. Plasmid gene mutation - (reply: 1)
  536. Clone blunt ended insulin gene into pbr322 - help (reply: 3)
  537. Gene Synthesis - Gene Synthesis (reply: 2)
  538. Who is my closest gene relative? - Help to determine phylogeny of a protein (reply: 2)
  539. RT-PCR for cloning low expressed genes - RT-PCR for cloning low expressed genes (reply: 1)
  540. how to find promoter region of a gene - (reply: 2)
  541. active genes and expression problems! - (reply: 3)
  542. Candidate gene methylation analysis - Some advice needed! (reply: 2)
  543. Problem about gene leakage in P.pastoris - (reply: 1)
  544. Method to look for novel methylated gene - (reply: 3)
  545. DNA arrays for low gene expression ? - (reply: 1)
  546. custom gene synthesis - (reply: 3)
  547. Which gene is the best internal control for RT-PCR - (reply: 5)
  548. cloning upper stream of a gene - (reply: 1)
  549. monitoring gene silencing - (reply: 7)
  550. How to get the promoter sequence of a gene - (reply: 4)
  551. Structure of insuline gene? - structure of insulin gene (reply: 2)
  552. Promoter Finder - How to find out promoter of known genes (reply: 2)
  553. RT-PCR primer design guide - How to check gene structure and design the primer? (reply: 15)
  554. clone - how to clone an unknown gene (reply: 1)
  555. What is a good control gene? - mRNA quantification..... (reply: 1)
  556. Housekeeping gene - (reply: 1)
  557. Cloning of high-GC% gene into vector - (reply: 3)
  558. Improve expression level in E. coli - How th improve expression of exogenuous gene in E. coli? (reply: 2)
  559. Gene copy number by PCR - (reply: 1)
  560. How can I clone the promoter region of my gene - (reply: 3)
  561. difficult expression of human gene in E. Coli - (reply: 3)
  562. How can I clone the upstream or downstraem of my gene?? - How can I clone the upstream or downstraem of my gene?? (reply: 1)
  563. deletion pcr - deletion pcr gene (reply: 3)