BioJob
BioBlog
PubAlert
BioTool
BioProduct
BioForum
Protocol
Home
Forum Index Home
Live Discussion
Top
:
Forum Archives
: :
Gene Related Discussions
no amplification in target gene -
(reply: 1)
Compare the expression of two target genes -
(reply: 3)
gene cloning questions -
(reply: 3)
need an example of a gene with a drug target.... -
(reply: 1)
House keeping gene copy number -
(reply: 3)
expression level comparison - a database to find gene expressi
(reply: 2)
"Gene expression vs Transcription vs mRNA levels": Help with conceptua -
(reply: 1)
gene expresion -
(reply: 1)
tagging of gene with GUS gene -
(reply: 1)
Gene complexity and protein complexity analysis -
(reply: 2)
DeltaDelta Ct Relative or Comparative Quantitation - methods of relative quantitation for qPCR determining gene expression
(reply: 3)
Why DNA sequencing does not detect deletions - help! - Med student lost on her hw. Breast cancer gene (BRCA1) mutations
(reply: 3)
can shRNA expression set share one polyA terminator with other gene -
(reply: 3)
Gene onthology software -
(reply: 1)
Tool to map genes on chromosomes -
(reply: 3)
mRNA Expression - How to observe mRNA expression of gene.
(reply: 10)
Looking for nucleotide sequence of rearranged antibody gene locus. -
(reply: 1)
Gene Knockout -
(reply: 2)
Best chemistry for relative quantitation of gene expression - Give your opinion on the best chemistry according to your experience
(reply: 5)
gene expression analysis -
(reply: 1)
housekeeping genes for brain tissue -
(reply: 4)
gene dowregulation by miRNA -
(reply: 1)
house keeping gene not intron spanning and sample DNase UNtreated -
(reply: 1)
B-catenin pathway - Lef/TCF-sensitive reporter gene assay
(reply: 4)
Help: JIGSAW- gene prediction -
(reply: 1)
Bulk extraction of genes information -
(reply: 1)
Identification of weakly expressed novel gene -
(reply: 1)
Bacterial gene disruption problem -
(reply: 2)
Can we measure gene expression with standard PCR? -
(reply: 1)
Can i use old standard curve to determine gene concentration? -
(reply: 1)
Cutting out EGFP gene from pEGFP-N1 - w/o using XbaI
(reply: 2)
qPCR - running taqman for endogenous control and SYBR for the gene -
(reply: 2)
housekeeping gene for B cell lines -
(reply: 1)
incorporation of restriction site in Primers - Help to design primer to clone gene in frame with fluorescent tag
(reply: 2)
lux gene cloning using Sal I as restriction enzyme -
(reply: 1)
how to check the insertion of a gene fragment into plasmid -
(reply: 2)
I want to find the promoter sequence of human IL-1 beta gene -
(reply: 1)
gene copy number - how to find how many?
(reply: 12)
Analyzing microarray data: a tool for annotations and grouping genes? -
(reply: 2)
Interpreting PCA Graph for Gene Expression - Principle Component Analysis in Gene Expression
(reply: 1)
RNAi of two different genes -
(reply: 4)
Finding correlated genes between microarray data sets -
(reply: 3)
which part of gene is needed for normal expression in vector -
(reply: 2)
How to target a gene for deletion -
(reply: 2)
how to check organ(tissue)-specific expression profile of a gene -
(reply: 3)
housekeeping gene for comparing gene expression between tumor and normal tissue -
(reply: 1)
How to identify novel gene using bioinfomatics -
(reply: 3)
PCR on this gene - products always present? -
(reply: 9)
Gene Deletion in E.Coli Bl21 -
(reply: 8)
what are 'floxed' genes in interbred mouse -
(reply: 1)
Does kanR gene confer resistance to Ampicillin? -
(reply: 1)
Siginificant Homology at the Amino acid level? - Gene sequence homology
(reply: 1)
Techanical Question about cloning of gene -
(reply: 2)
Ampiciline resistance gene -
(reply: 1)
Amplifying large gene -
(reply: 2)
NCBI gene sequence SOURCE? -
(reply: 7)
confirming how the gene causes decreased growth/increased cell death -
(reply: 4)
Confirmation of protein expression in pCDNA3 - How to know foreign gene expression in pCDNA3
(reply: 8)
Real time pcr in Rotor gene 6000- not getting positive results -
(reply: 1)
gene duplication - affected by siRNA?
(reply: 1)
Housekeeping genes for rat brain -
(reply: 4)
Chromosomal Gene Deletion - Gene Deletion by Double Crossing Over in Pseudomonas
(reply: 6)
Real-Time PCR and Housekeeping gene -
(reply: 9)
gene from 4-TOPO to expression vector -
(reply: 1)
help!real-time PCR to see gene's expression after treatment with cytokin -
(reply: 4)
New gene insertion in yeast - What are the suitable loci?
(reply: 3)
gene prediction tools -
(reply: 1)
histone modification-ChIP analysis - selection of proper internal control for repressed state of gene
(reply: 2)
Is this gene going to express? - is only the firs ATG read and express or all 3 reading frame?
(reply: 10)
gene hunting advice -
(reply: 2)
Genorm - Validation of housekeeping genes -
(reply: 1)
Can you get two proteins made from a heterozygous gene mutation? -
(reply: 3)
Affymetrix chip - unexpressed genes, which signal cuttof to use -
(reply: 1)
growing after filling in monolayer, please help - How and which genes/growth factors do it
(reply: 5)
gene was amplified when -RT were used as template? -
(reply: 4)
Cloning 2kb+ gene by PCR - Is it doable?
(reply: 3)
How to find miRNA precursor (or gene) for a specific miRNA -
(reply: 2)
primers dont match FASTA gene sequence... 'm confused -
(reply: 11)
BSP to test imprinted genes DNA methylation of oocytes - no specific or smear bands!
(reply: 4)
How to amplify 4.1Kb gene from genomic DNA? -
(reply: 15)
How to find promoter region of rat gene? -
(reply: 2)
comparing difference in gene expression -
(reply: 2)
software for CpG island prediction for a list of genes -
(reply: 3)
quantifying my gene expression in 5 different cell lines -
(reply: 1)
Does a gene have an associated CpG island? -
(reply: 9)
Finding a target gene sequence in rat genome using a mice gene - How to find the delta opioid receptor gene in rat if the sequence is k
(reply: 2)
Cloning toxic gene -
(reply: 2)
Scramble siRNA shows increase in gene expression by Taqman RTPCR -
(reply: 1)
Gene expression -
(reply: 1)
how to compare promoter regions from two genes -
(reply: 2)
neomycin-resistant gene vs kanamycin -
(reply: 3)
porcine CD4 gene sequence needed - urgently -
(reply: 2)
how to switch to a gene in a vector using PCR or restriction enzymes? - gene is in pIVS2 vector
(reply: 3)
Advice on analysing Differentially Methylated Regions of a gene. -
(reply: 5)
Genes/Proteins -
(reply: 6)
how to compare 2 different gene expression level in the same sample? - can I use ratio(A/B)=2^(CtB-CtA)to calculate these two genes' expr
(reply: 5)
How to find promoter region between two closely placed genes -
(reply: 1)
gene disruption - should regions flanking a cassette be of equal size
(reply: 2)
Induce mutation in my gene -
(reply: 5)
reporter gene fusion -
(reply: 3)
Bacterial housekeeping genes - What genes are commonly used as housekeeping controls in expression st
(reply: 1)
Reference Gene: human leukocytes -
(reply: 2)
rrn gene copy numbers -
(reply: 1)
Maximum acceptable variation of Housekeeping gene -
(reply: 1)
detection of mec A gene -
(reply: 1)
pcr for gene fusion -
(reply: 3)
Imprinted genes as a control? -
(reply: 1)
mutation in a gene that is not expressing a protein -
(reply: 4)
Long arm short arm of a gene/RF, not chromosome -
(reply: 1)
impossible gene ! -
(reply: 3)
integration vector question - do i need to clone a full gene
(reply: 1)
About housekeeping gene -
(reply: 3)
housekeeping genes -
(reply: 1)
Database of embryonic expressed genes -
(reply: 2)
problem with -ve controls of mt genes -
(reply: 1)
Need a weak promoter for reporter gene assay -
(reply: 3)
Cloning incomplete genes -
(reply: 2)
How to add a lethal gene to my plasmid for cloning selection? -
(reply: 3)
transcription of the genes in one operon -
(reply: 1)
ways to get a good gene amplification -
(reply: 4)
can cells just stop having the gene? -
(reply: 4)
how to amplify a long gene? -
(reply: 3)
Quorum sensing gene cloning -
(reply: 7)
Gene body methylation and active gene expression -
(reply: 6)
Replacing small gene with large gene -
(reply: 2)
Strategies for gene cloning and expression - Discuss various methods for it
(reply: 8)
two additional amino acids in gene -
(reply: 4)
Excision of foreign genes by E.coli - suspicion of attack
(reply: 5)
does size matter - how many genes in 1 vector
(reply: 10)
RNAi targeting one gene in a bicistronic transcript - Will the other gene be knock down?
(reply: 4)
RFLP versus a single gene to study genotypic diversity -
(reply: 4)
how to find the gene promoter region? -
(reply: 1)
how to get specific amplfication of a gene that is extremely similar to another -
(reply: 7)
How long does it take for the gene to lose? -
(reply: 2)
cDNA for real time PCR - for gene expression study
(reply: 1)
Creating Multimer Genes, cloning - cloning
(reply: 1)
Cloning of gene from peptide sequence -
(reply: 1)
transfer gene to protein? -
(reply: 3)
Used linear concatameric genes for transient transfection? -
(reply: 2)
how to know/predict a specific gene is regulated by miRNA -
(reply: 7)
PCR'ing a high GC gene -
(reply: 1)
which is the best approach for simultaneous siRNA of two genes? -
(reply: 4)
SDS page and gene cloning - pBS plasmid
(reply: 1)
subcloning dengue NS1 gene problems - molecular biology, cloning
(reply: 5)
Is it absolutely necessary to do BGS before doing MSP for new genes? -
(reply: 4)
GAPDH endogenous control gene -
(reply: 2)
Real Time PCR and Gene expression -
(reply: 1)
Inserting promoters upstrem of reporter genes -
(reply: 2)
where can I get any plasmid with aadA as selective marker gene? - i might offer some money
(reply: 1)
gene of interest or housekeeping gene shift...? -
(reply: 3)
Why is it difficult to PCR taget gene in rice successfully ? -
(reply: 1)
Express miRNA in vector for gene knockdown -
(reply: 4)
Relative expression of genes between bacterial species -
(reply: 1)
luciferase reporter gene assay -
(reply: 3)
RT-PCR of apoptosis related genes -
(reply: 2)
Is all lab bacterial strains have t7 RNA polymerase gene... -
(reply: 4)
amount of DNA for low copy number gene -
(reply: 1)
bacteria gene knockout using Red recombination system -
(reply: 1)
Reference genes on same plate as gene of interest? -
(reply: 2)
PCR clone a gene with known 3' end only -
(reply: 4)
where to find the list of genes known to be methylated -
(reply: 2)
semi-qRT-PCR - problem with housekeeping gene
(reply: 1)
change in expression of House keeping gene - change in expression of House keeping gene
(reply: 2)
primer dimer in pcr product - cloning for a gene. analyzing pcr product shows primer dimer
(reply: 8)
Replacing of GFP cloned inside a gene with RFP -
(reply: 2)
Problem PCR amplifying gene promoter regions -
(reply: 4)
official database for cancer-related genes -
(reply: 3)
how the gene cloned into a vector can expressed in mammalian cell without the 5& - What is the mechanism of translation initiaition with the HCM promoter?
(reply: 4)
3'UTR and Gene expression in expression construct -
(reply: 2)
qPCR with Corbett Rotor Gene 6000 -
(reply: 3)
PCR analysis of clones - Can a gene specific primer pair amplify a fragment in the wrong direction?
(reply: 1)
induction estrogen responsive gene (PS2 & PgR) in MCF7 -
(reply: 3)
co-transfection - co-transfection affect gene expression?
(reply: 4)
Induction of SAR - Resistant gene induction by salicylic acid
(reply: 1)
Seeking House keep gene under 200nt -
(reply: 5)
DNA methylation and gene silencing -
(reply: 5)
Disease gene clustered on chromosomes - Is there any truth to this?
(reply: 1)
How to find a gene's promoter sequence? -
(reply: 1)
How to study the expression of one, specific gene? -
(reply: 5)
How to get genes from a cytoband -
(reply: 1)
GFP as a reporter gene? -
(reply: 5)
RT-PCR a GC Rich (68%) gene (2200bp) -
(reply: 6)
house keeping gene -
(reply: 3)
software for gene designing -
(reply: 1)
Cloning of long gene -
(reply: 1)
toxic gene ligation/transformation URGENT HELP! -
(reply: 1)
What makes a gene a transcription Factor -
(reply: 4)
Positive Control for Methylation Studies in BSP sequencing - A "Universally" methylated Gene ??
(reply: 7)
Find promoter sequence of a gene -
(reply: 10)
multiple copies of gene or stronger promoter..? -
(reply: 2)
Reference Gene - How close is close? -
(reply: 4)
No success with PCR cloning of a gene into expression vector -
(reply: 5)
Which housekeeping genes for qRT-PCR control? -
(reply: 17)
coexpression of different genes in the same host -
(reply: 2)
5' RACE - gene specific primer -
(reply: 1)
Low PCR product for antibody gene -
(reply: 4)
Help: got the stable cell line without my target gene -
(reply: 3)
expressing a gene in a cell HELP -
(reply: 1)
DNA methylation analysis of the target gene -
(reply: 2)
Cloning gene behind GFP - Vector?
(reply: 2)
ask some question about gene expression data -
(reply: 1)
Problem with calculation using comparative method - When housekeeping gene is expressed less than sample
(reply: 1)
any site for CBF genes of arabidopsis -
(reply: 2)
Gene Copy Numbers -
(reply: 5)
Housekeeping gene for RT-PCR on plant - RT PCR
(reply: 1)
how to determine tissue specific expression of gene? -
(reply: 2)
Problem with QPCR - control gene does not amplify -
(reply: 2)
Gene3D db ? How can I visualise the nucleotides of a gene ? -
(reply: 1)
gene expression -
(reply: 2)
Molecular Basis of genes -
(reply: 3)
RT-PCR primers for E. coli LacZ gene -
(reply: 3)
RNAi in plant and detect with real time PCR - Detect gene expression decrease with qRT-PCR
(reply: 2)
How to retrieve a promoter sequences for a given gene ? -
(reply: 2)
Taqman for low levels of a gene (LacZ gene)in tissue -
(reply: 1)
gene promoter - promoter luciferase
(reply: 3)
determine the molecular mechanisms for gene regulation -
(reply: 1)
Some Suggestions for Systematic Gene Knockout pls -
(reply: 1)
Question about Nox gene -
(reply: 1)
gene orientation -
(reply: 6)
Help!Checking the nested Primer - P15 gene nested MSP
(reply: 2)
Which site of NDV NP gene is cut by BamHI and XhoI? -
(reply: 2)
Need Help; regulation of bacterial genes ? -
(reply: 3)
different siRNA for the same gene cause opposite effect -
(reply: 6)
Gene Expression Data - Looking for gene expression data
(reply: 2)
gene expression vs protein expression -
(reply: 3)
PCR of AmpR gene - PCR of Amp gene
(reply: 1)
Question about imprinted genes -
(reply: 3)
Cloned genes -
(reply: 1)
Can bacteria "store" gene products? -
(reply: 2)
Help!... Stable transfection.. - A293T with puromycin gene..
(reply: 3)
Vector Construction- Tagging N-term - necessity of ATG in Gene of Interest
(reply: 2)
suicide gene in bacteria - urgent help needed please.
(reply: 3)
Problem with expression of marker gene -
(reply: 2)
How to predict a promoter region for a gene - Promoter cloning + genes into vector
(reply: 6)
site directed mutagenesis - point mutagenesis of target gene
(reply: 3)
How would one go about moving a gene from plasmid to plasmid - cloning
(reply: 2)
Housekeeping Gene Selection / RNA purification troubles - Molecular Biology
(reply: 2)
Lower Ct for gene of interest than housekeeping gene -
(reply: 12)
Gene finder website -
(reply: 1)
5-AZA/TSA upregulation of gene in cell lines - Gene induction
(reply: 3)
overlap extension and clone gene into pET -15b - a question??
(reply: 3)
Gene expression comparison between different species(!) -
(reply: 1)
How to analyze a gene sequence? -
(reply: 2)
Cloning a gene without the sequence of the target species -
(reply: 3)
cloning genes with incompatible restriction sites - Protein expression
(reply: 2)
how to analyse the gene haplotypes -
(reply: 1)
QPCR - QPCR housekeeping gene
(reply: 1)
gene deletion via the RED recombinase method - are you experienced?
(reply: 3)
moc1 gene -
(reply: 2)
Normalizing protein expression under 24hrs ischemia - normalization with housekeeping genes
(reply: 5)
Internal control gene -Rt-qPCR -
(reply: 1)
PCR off of Genomic DNA problems! - -why is my gene without introns showing up?-
(reply: 3)
posible new gene -
(reply: 1)
I have a phenotype, but northern says gene is not knocked down - rnai knockdown
(reply: 2)
Gene expression in mammalian cells -
(reply: 6)
Gene expression - inducible Gene expression
(reply: 1)
RT-PCR for an intronless gene -
(reply: 6)
How important is cloning of full-length genes? -
(reply: 1)
software for drawing gene structure - please help!
(reply: 4)
AB resistance gene - Which antibiotic resistance gene in my vector ?
(reply: 3)
Get the gene by Geneinfo number -
(reply: 2)
Gene promoter -
(reply: 2)
Stable cell lines not overexpressing my gene of interest! - Technical problems
(reply: 2)
problems with PCR long target gene -
(reply: 2)
Transposon mutagenesis of Pseudomonas with Epicentre kit - Need a method for identifying the disrupted gene
(reply: 1)
How to find the transcription start site of an unknown gene? -
(reply: 1)
Reference gene -
(reply: 3)
Clone blunt ended insulin gene into pbr322 - help
(reply: 3)
Who is my closest gene relative? - Help to determine phylogeny of a protein
(reply: 2)
how to find promoter region of a gene -
(reply: 2)
Candidate gene methylation analysis - Some advice needed!
(reply: 2)
Method to look for novel methylated gene -
(reply: 3)
custom gene synthesis -
(reply: 3)
cloning upper stream of a gene -
(reply: 1)
How to get the promoter sequence of a gene -
(reply: 4)
Promoter Finder - How to find out promoter of known genes
(reply: 2)
clone - how to clone an unknown gene
(reply: 1)
Housekeeping gene -
(reply: 1)
Improve expression level in E. coli - How th improve expression of exogenuous gene in E. coli?
(reply: 2)
How can I clone the promoter region of my gene -
(reply: 3)
How can I clone the upstream or downstraem of my gene?? - How can I clone the upstream or downstraem of my gene??
(reply: 1)