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Gene Synthesis - Gene Synthesis (Oct/16/2004 )

I am a research associate and about to start with my project work. I would be working on gene synthesis so any guys who could help me with this would greatly appreciate. I have my protein sequences . What I need is to generate a  DNA sequence optimized for expression system of choice.

Which is the most  appropriate method for this- PCR assembly or LCR coupled to PCR ?

Also please let me know if there is any software available for oligo design?


Primer3 would help
good luck


There is also an academica software. It is probably published in Bioinformatics.