Gene Synthesis - Gene Synthesis (Oct/16/2004 )
I am a research associate and about to start with my project work. I would be working on gene synthesis so any guys who could help me with this would greatly appreciate. I have my protein sequences . What I need is to generate a DNA sequence optimized for expression system of choice.
Which is the most appropriate method for this- PCR assembly or LCR coupled to PCR ?
Also please let me know if there is any software available for oligo design?
Primer3 would help
There is also an academica software. It is probably published in Bioinformatics.