Kanamycin resistance gene in Gram-positive bacteria - (Nov/09/2008 )
Can the Kanamycin resistance gene from the Gram-negative vector used in Gram-positive vector? It'll take time if I order the Gram-positive bacteria for the Kanamycin resistance gene cloning or synthesize from oligos, so if the KmR gene from E. coli vector can be used, it'll be much easier and faster. Thank you in advance for any suggestion!
Should be no problem
Thank you, but is there any proof for that? I googled but I didn't find any work like this. Theoretically, the npt gene for KanR works in the cytoplasm, which is not much different between Gr+ and -, I think, but somehow sometime they don't work similarly. I just need a clue that someone already done it before to be confident performing it.