cloning vector for high GC ratio gene? - is GC ratio a criteria in choosing vector?? (Dec/06/2007 )
Hi. I'm trying to express a phytase gene which has a high GC ratio in E. coli. The thing is, I'm not sure if the ratio of GC is a criteria in choosing the vector or rather, if there are specific plasmids that are more suited to expression of these sort of genes. Any suggestions?
I am not sure one would choose a vector over another because of high GC content. But one might choose different cells to grow the plasmid in.
Yeah, I haven't heard of vectors for GC-rich genes, that's more of a bacterial strain thing to prevent recombination or deletion of sequences that "loop out" of your plasmid. SURE cells from Stratagene are good for preventing that.