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multiple copies of gene or stronger promoter..? - (Apr/07/2006 )


what is preferable for higher production of a recombinant product…multiple copies of gene (high copy number) or stronger promoter..??
what is the advantage and disadvantage of each one..???
thank you


I would say both !
however if your protein is toxic for the bacteria, a low copy, with a strong inducible promoter is better.


Both is accurate depending on what you're doing. It can be harder to control expression with copy number. I you just need protein it fast and easy way to get robust quantities (if you don't kill your host). A tight promoter or promoter with sensitive inducibility can be very valuable for expression studies.