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gene delivery lipofectamine - lipoplexes acetone (Jan/19/2007 )

hi i have some basic dumb questions,
I treated lipoplexes (Lipofectamine + DNA) with acetone. I dried acetone, then did transfection. I found the transfection efficiency dropped with the samples treated with acetone, compared to the fresh ones. How to explain this?
what effect acetone can have on liposomes and lipoploplexes?


my question is : why did you treat them with acetone?... did you have a reference for such treatment?


You probably dissolved free liposomes as well as extracted some lipid components from DNA-lipid complexes that are critical to the transfection efficiency. In addition, the particle sizes may be changed after treatment.


what is ur goal here, to achieve higher % of transfection? As u have already experienced, leave out the acetone. i dont know anyone suggesting using acetone for transfection with lipofectamine. I dont remember having read abt it in the lipofectamine protocol.

And as these transfection reagents r lipid based, acetone probably interferes with their structure to reduce transfection efficiency.


no no no i don't use actone+lipofectamine to do transfection everytime.I will disperse polyplexes in a polymeric solution. Polymer for example, PLGA polylactideglycolide. These polymers are soluble only in solvents like acetone/chloroform. so my aim is to study the effect of solvents on lipoplexes, before i place them in the polymer solution.
i too susp[ect that there may be an increase in size, apart from that will there be any chemical interaction between lipoplexes and acetone?