gene delivery lipofectamine - lipoplexes acetone (Jan/19/2007 )

hi i have some basic dumb questions,
I treated lipoplexes (Lipofectamine + DNA) with acetone. I dried acetone, then did transfection. I found the transfection efficiency dropped with the samples treated with acetone, compared to the fresh ones. How to explain this?
what effect acetone can have on liposomes and lipoploplexes?

-mini-

my question is : why did you treat them with acetone?... did you have a reference for such treatment?

-fred_33-

You probably dissolved free liposomes as well as extracted some lipid components from DNA-lipid complexes that are critical to the transfection efficiency. In addition, the particle sizes may be changed after treatment.

-genehunter-1-

what is ur goal here, to achieve higher % of transfection? As u have already experienced, leave out the acetone. i dont know anyone suggesting using acetone for transfection with lipofectamine. I dont remember having read abt it in the lipofectamine protocol.

And as these transfection reagents r lipid based, acetone probably interferes with their structure to reduce transfection efficiency.

-scolix-

no no no i don't use actone+lipofectamine to do transfection everytime.I will disperse polyplexes in a polymeric solution. Polymer for example, PLGA polylactideglycolide. These polymers are soluble only in solvents like acetone/chloroform. so my aim is to study the effect of solvents on lipoplexes, before i place them in the polymer solution.
i too susp[ect that there may be an increase in size, apart from that will there be any chemical interaction between lipoplexes and acetone?

-mini-