qPCR with Corbett Rotor Gene 6000 - (Jul/07/2006 )

I needed to switch fom an ABI 7700 system to a Corbett Rotor Gene. I am using SYBR Green jump start master mix (Sigma). I am using a Corbett Robot to pipett a 10 microl reaction. Now, I am not very happy with the dublicates and the amplification curves.

Should the flourescence not be 100? Some of my colleges get curves around 20, that cant be right?

Can make any suggestions what I can do to improve my assay?

Many Thanks.

-peetyswoo-

QUOTE (peetyswoo @ Jul 7 2006, 11:59 PM)

I needed to switch fom an ABI 7700 system to a Corbett Rotor Gene. I am using SYBR Green jump start master mix (Sigma). I am using a Corbett Robot to pipett a 10 microl reaction. Now, I am not very happy with the dublicates and the amplification curves.

[attachment=1287:attachment]

Should the flourescence not be 100? Some of my colleges get curves around 20, that cant be right?

Can make any suggestions what I can do to improve my assay?

Many Thanks.

It seems fairly normal compared to the results I get on our Corbett 6000. Did your previous system normalise to 100 fluorescence? Also, your duplicates look awesome, you are only interested in the exponential part of the graph/reaction (right at the bottom).

-maset-

That's great...

By the way, what is the approx. price of the Corbett Rotor Gene 6000?

Thanks

-AmonTobin-

Something like 34 to 35 thousand USD with 6 channel

QUOTE (AmonTobin @ Nov 16 2006, 01:20 AM)

That's great...

By the way, what is the approx. price of the Corbett Rotor Gene 6000?

Thanks

-KFC-