how to analyse the gene haplotypes - (May/30/2005 )
hi every one, i'm a newcomer and any help will be appreciated.
i'v sequenced some PCR products. Now I want to analyze the haplotypes, but how? i don't konw, especially the heterozygotes. although there are many combinations in heterozygote, but only 2 haplotypes in each heterozygote. so how can i find them? 
-penicilinum-
QUOTE (penicilinum @ May 30 2005, 11:14 AM)
hi every one, i'm a newcomer and any help will be appreciated.
i'v sequenced some PCR products. Now I want to analyze the haplotypes, but how? i don't konw, especially the heterozygotes. although there are many combinations in heterozygote, but only 2 haplotypes in each heterozygote. so how can i find them?
i'v sequenced some PCR products. Now I want to analyze the haplotypes, but how? i don't konw, especially the heterozygotes. although there are many combinations in heterozygote, but only 2 haplotypes in each heterozygote. so how can i find them?
Hi, I also have a similar problem. In general, when cloning the two different haplotypes in heterozygotes should be easily recognisable from alignment of the clones sequences. I have, however, a strange recombination event during PCR. Maybe the incompletely extended strands from one haplotype act as primers on strands on the other haplotype, giving rise to "recombinant" haplotypes. Does anyone have any idea to solve this problem? I simply tried to increase extension time, both at each cycle and at the final extension. It didn't work...
Any advice?
Wally
-wally-