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Cutting out EGFP gene from pEGFP-N1 - w/o using XbaI (Jul/19/2008 )

anyone who ever cut out EGFP gene without using XbaI at 3´ of pEGFP-N1 vector?

-The Bearer-

i pcr'd it up with primers tagged with my restriction enzymes of interest.....cheating i guess, but easier!

QUOTE (The Bearer @ Jul 20 2008, 07:23 AM)
anyone who ever cut out EGFP gene without using XbaI at 3´ of pEGFP-N1 vector?

-aussieuk-

I used NotI...

-stardust-