Protocol Online logo
Top : Forum Archives: : Molecular Biology

gene linearization for transfection - stable mammalian tranfection. (Feb/08/2005 )

My viral vector (pBabe) must be linearize before transfection
- with CLONfectin?
- with electroporation
thank you


for electroporation, and transfection, there's no need to linearize your vector. Be sure adding a selection pressure (like antibiotic) two days after transfection in order to eliminate cells that don't have integrated your vectorin their genome.