Looking for nucleotide sequence of rearranged antibody gene locus. - (Oct/15/2008 )
I'm trying to get the sequence of an rearranged antibody gene locus.
Specifically i need to find the sequence, either protein or nucleotide, of a part of an anti-CD41 or anti-CD42 a/b/c/d antibody.
In fact I can't seem to find antibody sequences in general except for conserved, consensus regions. Funny thing is i need the Variable heavy and light chains, more specifically even just the epitope binding sequences, short as they are.
Aside from creating a b cell line that produces antibody to CD41/42 and then cloning, PCR, and sequencing (which i don't want to do), what resources exist that can give me this information??
take a look at the IMGT website