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Primer Related Discussions
  1. Can someone help me on my primer design? (contains flag tag) - (reply: 2)
  2. testing primers efficiency for rt-PCR - (reply: 1)
  3. Primer sequences with M and Y - how to order? - (reply: 2)
  4. need help calculating stock primer concentration - (reply: 1)
  5. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
  6. methylation specifc primers thermal cycle conditions - (reply: 4)
  7. PCR Amplification Issues and Primer dimer - (reply: 4)
  8. Primer calculation for qPCR - final reaction - (reply: 1)
  9. Sec. structure in primers - (reply: 1)
  10. PCR master mix containing primers and Taq - (reply: 4)
  11. Primer working with some cDNA samples and not other - (reply: 2)
  12. Primer dilution - (reply: 7)
  13. why not working msp primers - (reply: 4)
  14. Volume of Primers - (reply: 4)
  15. Primer Designing - (reply: 9)
  16. pcr primer design - (reply: 6)
  17. Please help (primer/ probe final volume calculations) - (reply: 1)
  18. Primer design with EcoRI ends - (reply: 2)
  19. PCR with overlapping primers only (primer extention) - (reply: 3)
  20. MSP Primer design - (reply: 5)
  21. How can I check the quality of bacterial primers and their specificity on blast? - (reply: 1)
  22. Primer design - (reply: 1)
  23. Primer non specific binding sites - (reply: 7)
  24. Degenerate primers - (reply: 8)
  25. Primers with 2 different annealing temp - (reply: 1)
  26. Y-linker transgene integration site PCR primer verification - (reply: 1)
  27. NEB TM CALCULATOR PRIMER CONCENTRATION - (reply: 2)
  28. primer design for a gene - (reply: 3)
  29. confirmation for primers dilution - (reply: 10)
  30. Possible primer dimer problem..Need solution!!! - (reply: 2)
  31. confusing with where to put primers and which ordination - (reply: 4)
  32. Help with primer concentration for sequencing - (reply: 1)
  33. Design the primers - (reply: 10)
  34. real time PCR primer design - (reply: 1)
  35. primers for cloning - (reply: 1)
  36. Degenerated Primers and their problems - (reply: 1)
  37. primer design with restriction enzyme - (reply: 1)
  38. Interpreting primer BLAST scores for self-complementary - (reply: 5)
  39. primer dilution help - (reply: 1)
  40. Testing primers on 'unknown' tissue - (reply: 1)
  41. could you help me with my stem loop RT primer? - (reply: 1)
  42. excess amount of primers - (reply: 2)
  43. Dehydrating and Reconstituting primers - (reply: 15)
  44. KIR gene promoters for MSP primers design - (reply: 2)
  45. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
  46. Primer as limiting reagent in PCR reaction - (reply: 2)
  47. Primer Design for RNA probes - (reply: 2)
  48. Nested BSP Primer Design - (reply: 6)
  49. website for primer design - (reply: 1)
  50. How to do a primer dilution - (reply: 10)
  51. Primer design and alternative transcripts - (reply: 2)
  52. NCBI Primer Design - Stringency Issues - (reply: 3)
  53. Different primer concentration in qPCR - (reply: 1)
  54. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
  55. Why when disigning primers for RTPCR, you need to chose a region where introns a - (reply: 1)
  56. Primer Specificity: Testing only one primer - (reply: 4)
  57. tool for comparing many primers pairs - (reply: 4)
  58. Pcr primers - (reply: 7)
  59. Primers have worked well but now getting primer dimers? - (reply: 2)
  60. I cannot design primers on exon-exon junction - (reply: 2)
  61. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  62. Designing primers in UTRs - (reply: 1)
  63. primer design@ buy? - (reply: 2)
  64. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
  65. designing primers( selecting target sequence/amplicon design) - (reply: 3)
  66. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  67. How to design primer to amplify genomic DNA? - (reply: 3)
  68. Primers - (reply: 1)
  69. Primers mix - (reply: 2)
  70. Having problem with primers for qPCR - (reply: 4)
  71. Troubleshooting methylation primers for Bio-Rad PCR - (reply: 3)
  72. No bands despite different primers and conditions and TAqs - (reply: 8)
  73. Equimolar Mix Primer - (reply: 8)
  74. Primer design - (reply: 5)
  75. storage for lyophilized primers - (reply: 1)
  76. Repeated mutagensis primer in site-directed mutagenesis - (reply: 8)
  77. PCR Primer trouble - (reply: 2)
  78. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
  79. finding the corrosponding primers - (reply: 2)
  80. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
  81. Designing primers for a nuclear region to amplify in a species that has no nucle - (reply: 1)
  82. T7 and M13 primers two band amplification - (reply: 3)
  83. fluorescent primer vs fluorescent terminator in sequencing - (reply: 4)
  84. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
  85. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
  86. Melting curve is irregular for primer optimization - (reply: 5)
  87. Designing primers for ABO blood groups - (reply: 1)
  88. RT-PCR primer design - (reply: 7)
  89. Is this primer okay? - (reply: 4)
  90. Can I use Primer as template? - (reply: 1)
  91. Resuspending primers calculation - (reply: 4)
  92. What do you use to check primer secondary structure? - (reply: 3)
  93. mutagenic primers with very high GC content. - (reply: 3)
  94. site-directed mutagenesis primer Tm - (reply: 4)
  95. Random vs oligo primer in preamplification RT - (reply: 1)
  96. Primer concentration - stupid question - (reply: 3)
  97. Primer Design, help im New - (reply: 4)
  98. RT-qPCR primer problem - (reply: 4)
  99. [Video] Using NCBI for RT-PCR Primer Design - (reply: 1)
  100. Designing cloning primers for DNMT - (reply: 2)
  101. Are these primer products good enough for qPCR? - (reply: 3)
  102. Can't get PCR with large overhang primers to work - (reply: 8)
  103. Primer Efficiency across runs - (reply: 1)
  104. Designing PCR Primers for cloning - (reply: 17)
  105. Creating primers to add restriction sites to vector backbone - (reply: 7)
  106. Some primers dose not work - (reply: 5)
  107. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
  108. Query regarding primers for quick change mutagenesis - (reply: 3)
  109. the storage time for primers - (reply: 9)
  110. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  111. random primers or oligodT - (reply: 4)
  112. To design or use published primers? - (reply: 4)
  113. PCR primer usage (Clonning & cDNA) - (reply: 2)
  114. problem in cloning PCR primer design with restriction site - (reply: 4)
  115. Amplification with U primers, but not with M primers - (reply: 1)
  116. His tag introduction in to gene and primer design - (reply: 2)
  117. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
  118. Design primer from incomplete sequence... - (reply: 2)
  119. Design primer from incomplete sequence... - (reply: 2)
  120. Primer designing for Methylation - (reply: 6)
  121. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
  122. Overlapping sequence PCR primers - (reply: 1)
  123. BSP PCR primer design explained - (reply: 11)
  124. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
  125. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
  126. good 16S univ. primer for qPCR? - (reply: 3)
  127. I need help for designing a pair of primers for this sequence. - (reply: 1)
  128. long primers PCR - (reply: 4)
  129. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
  130. Sequencing Primers and Plasmids - (reply: 3)
  131. Primers to amplify Kan operon from pet28 - (reply: 2)
  132. Tagged primers and a second PCR targetting the tags - (reply: 5)
  133. Barcode generator for PCR primers - (reply: 2)
  134. Biotinylated primers - (reply: 4)
  135. Transcription of PCR products without a primer coupled with promotor sequence - (reply: 4)
  136. How to join two sequencing files taken form forward and reverse primer - (reply: 3)
  137. Control primers for bisulfite-converted DNA - (reply: 3)
  138. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
  139. Is 27F and Anti-Gamma Primer the same thing for 16s? - (reply: 9)
  140. Q regarding primer mix for MSP!! - (reply: 4)
  141. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  142. BSP Primers with M13 Tails - (reply: 1)
  143. issues when designing PCR primers using PRIMER3 - (reply: 8)
  144. Primer catalog/databasing - (reply: 1)
  145. Primer Blast - (reply: 6)
  146. Help designing primers for use in In-fushion cloning - (reply: 4)
  147. Primers Freeze-Thaw - (reply: 16)
  148. primer design using 16s rRNA - (reply: 1)
  149. Primer concentration for cDNA synthesis using GSP - (reply: 1)
  150. Primer Design HELP! - (reply: 3)
  151. Bisulfite PCR doesn't work and the primer seems abnormal - (reply: 6)
  152. How are Primers made? - (reply: 9)
  153. designing methylation specific primers - (reply: 3)
  154. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  155. Primer Annealing Temperatures - (reply: 24)
  156. same primers, different product in conventional PCR and qPCR - (reply: 6)
  157. Different primer optimalization for nested vs direct MSP? - (reply: 3)
  158. Primers amplify genomic DNA but not cDNA - (reply: 2)
  159. Primer BLAST - (reply: 1)
  160. PCR primer design - published primers trustable? - (reply: 8)
  161. question about universal and specific primers for miRNA real time pcr - (reply: 1)
  162. Can we use degenerate bases in BSP primers? - (reply: 1)
  163. How to check primer sequences using BLAST and other tools? - (reply: 2)
  164. Primer Check? - (reply: 2)
  165. Validation of PCR primers/ probes - (reply: 6)
  166. Two reverse primer sequences for a single forward primer - (reply: 1)
  167. PCR with more than one primer - (reply: 1)
  168. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  169. RT-PCR primer design - (reply: 1)
  170. No or very weak band using 16s primers - (reply: 1)
  171. No PCR amplification with b-actin primers - (reply: 1)
  172. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
  173. Primer Design Help: GFP primers for Arabidopsis - (reply: 1)
  174. No PCR amplified with long primers - (reply: 10)
  175. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  176. another primer dilution question - (reply: 2)
  177. Primer Efficiency - (reply: 2)
  178. oligo(dt) 15 vs random primers - (reply: 3)
  179. primer dilutions - (reply: 4)
  180. Genotyping PCR primers - (reply: 1)
  181. MSP Primers not working - (reply: 6)
  182. Primers too dilute - (reply: 1)
  183. Cloning HELP- Possible Primer issues - (reply: 6)
  184. PCR primer pairs - university exam - (reply: 7)
  185. Help with primer design through PRIMER3 - (reply: 4)
  186. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  187. Tm of primers - prediction and verification - (reply: 1)
  188. how to design primers for 16sr RNA - (reply: 24)
  189. Help with primer design - (reply: 3)
  190. Primer Tm calculation. - (reply: 1)
  191. Running Primers on Agarose Gel - (reply: 6)
  192. PCR product now not getting with same primers and other PCR conditions - (reply: 4)
  193. universal primers for PCR ribotyping - (reply: 6)
  194. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  195. Primer design: Tm above or below Ta? - (reply: 5)
  196. Oligo primers for shRNA construction - (reply: 1)
  197. Design primers for expression cloning - (reply: 11)
  198. primer dilution query - (reply: 2)
  199. Using BLAST to check primers - (reply: 1)
  200. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  201. What is the function of a third primer in a PCR mix?? - (reply: 2)
  202. How can we design a primer ? step by step method - (reply: 4)
  203. EPIGENETICS-NEWBIE-Weird/too close primers for pyrosequencing assay - (reply: 2)
  204. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
  205. Is it possible to amplify cDNA with hexamer primers or oligo dT primers to have - (reply: 1)
  206. annealing temperature for 3 primer pairs? - (reply: 4)
  207. Impact of primer sequence on TA cloning - (reply: 3)
  208. Pcr primers - (reply: 2)
  209. RT-Q PCR primer design question - (reply: 2)
  210. multiplex rtqPCR and custom primer/probe design - (reply: 2)
  211. big differnece in my primers Tm - (reply: 2)
  212. Designing primers for the ORFs - (reply: 9)
  213. PCR with phophorylated primers - (reply: 1)
  214. Problem with New qPCR primers - help! - (reply: 6)
  215. Designing primers for miRNA located on minus strand. - (reply: 2)
  216. Plasmid sequencing without primers - (reply: 1)
  217. RT-PCR primers and the poly(A) tail - (reply: 2)
  218. overlapping primer - (reply: 1)
  219. PCR primers help - (reply: 5)
  220. Primer dimers - (reply: 2)
  221. Primer validation and PCR efficiency - (reply: 4)
  222. should I design primers spanning both intron and exon - (reply: 2)
  223. PCR with a very long and a short primer - (reply: 5)
  224. Comparison between two primers pairs? Is it possible? - (reply: 3)
  225. Rt-PCR primer design - (reply: 7)
  226. Length of non-binding sequence in primers with RE site - (reply: 6)
  227. Orientation of primers - (reply: 2)
  228. methylation analysis with MS-HRM (primer design) - (reply: 1)
  229. primer resuspension - (reply: 2)
  230. Help designing primers with restriction sites - (reply: 3)
  231. Why primers shouldn't have Tm difference greater than 5C? - (reply: 5)
  232. PCR primer with no extra DNA end - (reply: 5)
  233. Tm calculation for primers with RE sites and overhangs - (reply: 4)
  234. Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
  235. checking msp primers - (reply: 2)
  236. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  237. Another primer dimers problem - (reply: 23)
  238. MSP U primers very difficult to amplify... - (reply: 4)
  239. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
  240. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  241. Primer design - (reply: 3)
  242. single primer PCR - (reply: 5)
  243. Primer concentration - (reply: 4)
  244. Primer 3' mismatch - Strange experience (reply: 9)
  245. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
  246. PRIMER DILUTION - STOCK SOLUTION TO A WORKING STANDARD (reply: 2)
  247. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
  248. Are my MSP primers good? - MSP primers design by Methyl Primer Express v1.0 (reply: 2)
  249. Primer dilution --> problem.. - (reply: 16)
  250. Sybr green RT-qPCR primer - (reply: 1)
  251. Sybr green RT-qPCR primer - (reply: 1)
  252. primer design - microalgae (reply: 2)
  253. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
  254. how to overcome primer contamination - need protocol (reply: 11)
  255. RT-PCR primer design - Primer design and evaluation (reply: 7)
  256. STANDARDISATION OF MULTIPLEX PCR - IN order to standardise a multiplex PCR for 3 set of primers (reply: 3)
  257. Primer,probes dilution - (reply: 3)
  258. PCR: Cloning Primers - (reply: 8)
  259. Exon Spanning PCR Primers - (reply: 1)
  260. Primer sequences - (reply: 1)
  261. PCR Primers - (reply: 2)
  262. How to blast methylation specific and unspecific primers - (reply: 1)
  263. How to check primers are of correct sequence or not? - (reply: 2)
  264. Primers... - (reply: 1)
  265. When are primer dimers a problem? - (reply: 1)
  266. primer with higher melting temperature - (reply: 2)
  267. Problems designing primers for BSP - (reply: 2)
  268. qPCR primer design - possible off-target (reply: 1)
  269. Primer dimer formation and real-time PCR - (reply: 7)
  270. Primer Design Urgent! - (reply: 1)
  271. how to know my primer's sense ??? - help ! (reply: 2)
  272. intron spanning primers for non model organisms - (reply: 2)
  273. Primer design - (reply: 2)
  274. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
  275. Primer seq - (reply: 2)
  276. oligo dt and random primer - (reply: 3)
  277. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
  278. Primers and self and hetero dimers - (reply: 1)
  279. Primers for qPCR - (reply: 3)
  280. RT-PCR primer - (reply: 3)
  281. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
  282. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  283. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  284. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  285. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  286. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  287. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
  288. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
  289. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
  290. ChIP primer design - (reply: 1)
  291. Bisulfite sequencing primer design query (probably simple) - (reply: 4)
  292. Question about Primers - (reply: 3)
  293. Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
  294. designing MSP primers (dimers NOOOOOOOO!) - (reply: 1)
  295. Primer design with a tag - (reply: 4)
  296. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
  297. Combining forward and reverse primer gives different size on gel - (reply: 2)
  298. primers for fungal identification - (reply: 9)
  299. difference between PCR primers and sequencing primers - (reply: 1)
  300. Sense and antisense DNA, and primer design - (reply: 6)
  301. Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
  302. Question about RealTime PCR Primer Design - (reply: 1)
  303. Designing a Primer with a RS that has a W - (reply: 1)
  304. Primers stopped working!? - PCR Primers (reply: 6)
  305. NFK beta primers - (reply: 4)
  306. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
  307. Primer design - Primer design (reply: 2)
  308. Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
  309. Real Time PCR Primers and Probes - (reply: 2)
  310. Need help - How we can design primer for miRNA (reply: 1)
  311. primer and promoter sequences - (reply: 1)
  312. designing of primers - (reply: 2)
  313. Primer Tm differences/ PCR - (reply: 1)
  314. Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
  315. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
  316. d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
  317. Designing primer to remove his-tag - (reply: 1)
  318. PCR Primer Dilution from F+R - (reply: 1)
  319. primer reconstitution - primer reconstitution (reply: 4)
  320. Primer design for insert amplification - Is this correct? (reply: 2)
  321. HRM primers - (reply: 1)
  322. primer design - (reply: 2)
  323. New to sequencing, primer design - (reply: 2)
  324. Quickchange mutagenesis primer - (reply: 3)
  325. pcr / primer theory in bisulfite sequencing - (reply: 4)
  326. Primer Design..need very basic advice. - Please help, I cant figure this out for the life of me. (reply: 2)
  327. Problems with the Specificity of the Primers - (reply: 2)
  328. confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
  329. Degenerate primers PCR problem, Please help! - (reply: 2)
  330. Primer design help needed - (reply: 1)
  331. Primers no longer work - (reply: 1)
  332. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
  333. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
  334. Calculating Primer concentrations for PCR - Is there an easy way to do this...help (reply: 3)
  335. primer Tm is too high, how tu get pcr product - help! (reply: 21)
  336. Find universal primers in vector - any online tool? (reply: 6)
  337. Picking primers to confirm Illumina meth27 results - (reply: 2)
  338. Colony Pcr - primers - (reply: 5)
  339. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
  340. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  341. Primer Dilution Problem - D'oh! (reply: 2)
  342. Is there any software that can be sued to design degenerate primers - (reply: 1)
  343. Primer Decontamination - (reply: 2)
  344. tools for checking non-specific binding of primers -
    (reply: 1)
  345. Primer designed for ARMS PCR - (reply: 2)
  346. methylation specific PCR - Primers - (reply: 1)
  347. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
  348. Primer Design - aligning sequences for probe/primer design (reply: 2)
  349. PCR with one primer - (reply: 1)
  350. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
  351. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
  352. High Tm on primers - cannot get a product - (reply: 8)
  353. NCBI primer blast problem - (reply: 1)
  354. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
  355. primer sequence problem - (reply: 2)
  356. Finding bisulfite PCR primer sequence - (reply: 3)
  357. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
  358. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
  359. How to design primers to check my candidates in a ChiP assay - (reply: 4)
  360. Primer Reconstitution--does temperature matter? - (reply: 5)
  361. Primer Design MSP, BSP, MS-HRM - (reply: 3)
  362. Primers that do not align at regions with SNPs. Where to find SNPs? - (reply: 2)
  363. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  364. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
  365. Primer design for qPCR - (reply: 2)
  366. Primer design for qPCR - (reply: 1)
  367. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
  368. primer design tips - (reply: 3)
  369. design pcr primer - (reply: 1)
  370. PCR with long and complex primers - (reply: 2)
  371. Phospholylation of primers - (reply: 1)
  372. LINE1 OFR2 Primer Design - (reply: 1)
  373. phosphorylation of primers using PNK - (reply: 1)
  374. Primers for yeast (18S) - (reply: 1)
  375. What's the longest overhanging primer seq. you have used? - (reply: 2)
  376. Primer Concentration to lower Ct - (reply: 1)
  377. Primer Concentration Help - (reply: 4)
  378. Primer Degradation - What exactly happens? (reply: 1)
  379. Primers in 2 exons - (reply: 2)
  380. Problems with designing a primer - (reply: 2)
  381. Probe or Primer info for bovine AQP-1 - (reply: 6)
  382. Different sized product for same BS primer! - (reply: 2)
  383. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  384. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
  385. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  386. primers with restriction site - (reply: 1)
  387. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
  388. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
  389. primer design by generunner DG - (reply: 1)
  390. BSP primers design, help please - (reply: 2)
  391. primers with fluoresent dye PCR cycle question - (reply: 4)
  392. Urgent! Sequencing a mixture of ssDNA fragments of the same gene - ssDNA different in size amplified from a single primer (reply: 2)
  393. Problems with validating primers and low expression genes - (reply: 3)
  394. intron/exon spanning primers - (reply: 1)
  395. MSP primer troubles - (reply: 3)
  396. Designing Primers for multiple Isoforms - (reply: 1)
  397. real time pcr melt curve and primer efficiency problem - (reply: 3)
  398. how to design primer for my n-terminal sequence - hot to design primer for my protein (reply: 2)
  399. Addition of Restriction sites into PCR primers - (reply: 4)
  400. Primer Design in 3non translated version vs. coding region - (reply: 2)
  401. different PCR primers for real-time and classic PCR - (reply: 3)
  402. primers deconamination??? - (reply: 6)
  403. primer check!!! - (reply: 6)
  404. Annealing Temperature of biotinylated primers - (reply: 2)
  405. primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
  406. Primer clean-up - ExoSAP-It isn't working (reply: 6)
  407. Primer dilution - PCR primer dilution (reply: 2)
  408. Long Primers for PCR - (reply: 2)
  409. Primers going "loopy" - (reply: 2)
  410. Conversion of primer unit - ug/ul to uM (reply: 4)
  411. Primer Trouble Shooting - (reply: 5)
  412. Proven Primers Stopped working & now leave smear! - (reply: 2)
  413. a simple primer problem - (reply: 5)
  414. Primers from 3'UTR - (reply: 2)
  415. Designing primers with ESTs - (reply: 2)
  416. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
  417. addition of BclI restriction site to PCR primer - (reply: 6)
  418. How does methprimer calculate primer Tm - (reply: 2)
  419. Phosphorylated primers - (reply: 5)
  420. QPCR - Primer and Probe question - QPCR (reply: 2)
  421. Easy primer question? - Primers (reply: 1)
  422. primer dimer - proplems (reply: 1)
  423. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
  424. Primer efficiency test - (reply: 1)
  425. primer software - (reply: 2)
  426. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
  427. How to know in which exon a primer match? - (reply: 1)
  428. primer annealing - higher annealing= less wrong bounds (reply: 4)
  429. sequencing with forward/reverse primers - (reply: 7)
  430. Adding A overhangs - primer design implications? - (reply: 1)
  431. primer RE over-hang nucleotides - common sequences? (reply: 5)
  432. Forward and reverse primers got very different Tm - what to do? (reply: 11)
  433. primer design problem - (reply: 1)
  434. Problem with primer efficiency analysis - (reply: 4)
  435. primer design - (reply: 1)
  436. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
  437. designing primers for genes not sequenced yet - (reply: 2)
  438. Primer desing - (reply: 2)
  439. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
  440. primer design - (reply: 9)
  441. primer contamination - primer contaminated with ice (reply: 3)
  442. Primer3 vs Primer BLAST - (reply: 3)
  443. Primer design: free energy - (reply: 2)
  444. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  445. Primer optimization for ChIP - (reply: 2)
  446. sequencing primer: T7 or T7promoter? - (reply: 4)
  447. primer tm - tm calculation for tagged primers (reply: 2)
  448. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
  449. Universal 16s rRNA primers needed - (reply: 3)
  450. PCR primers for histone mod ChIP - How to find the regions (reply: 1)
  451. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
  452. adding koazak sequence to primer - (reply: 1)
  453. Designing primers for cloning - After primer designing, how should I perform the PCR? (reply: 15)
  454. Primer design if sequence is unknown for organism - (reply: 5)
  455. adding C terminal tag to reverse primer - (reply: 1)
  456. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
  457. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
  458. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
  459. what primers should I use for DNA sequencing? please help - (reply: 2)
  460. what primers should I use for DNA sequencing?? - (reply: 3)
  461. Is my primer going to work for qRT-PCR? - (reply: 6)
  462. Primers annealing temperatures - (reply: 8)
  463. Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
  464. Synthesize own Oligo-dT primers - (reply: 6)
  465. Testing primer specificity... - ...without gDNA of target organisms (reply: 6)
  466. primer design - Primer design for PCR (reply: 1)
  467. How are primers designed? - (reply: 4)
  468. need help on primer calcuation soon - (reply: 1)
  469. primer design - (reply: 6)
  470. 23S rRNA Primer - need primer sequence (reply: 1)
  471. Freezing primers+SYBR green - (reply: 2)
  472. Tagged Primers - (reply: 5)
  473. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
  474. design primer for chip - (reply: 5)
  475. How to quantify oligonucleotide primers using nanodrop ND-1000 software - newbie help (reply: 2)
  476. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  477. reconstitution of primers - (reply: 2)
  478. primer dimer - (reply: 2)
  479. PCR with long primers - (reply: 3)
  480. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  481. Help me! How to design nested BSP primers? - (reply: 4)
  482. Using BLAST to check primers - (reply: 17)
  483. PCR primer - (reply: 2)
  484. Primer design - GC clamp - (reply: 8)
  485. primer dimer or what? - (reply: 2)
  486. Primers and Taqman Probes mixture question - (reply: 1)
  487. designing primers - (reply: 1)
  488. Negative Control Primers for ChIP Assay - (reply: 3)
  489. PCR Cloning-large primers - (reply: 4)
  490. forward and reverse primer - (reply: 4)
  491. Check the primers - (reply: 4)
  492. BSP primers - (reply: 2)
  493. Primer dimer issue in real time PCR - (reply: 21)
  494. RT-PCR primer dimers and cDNA degradation - (reply: 2)
  495. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  496. primer binding - (reply: 1)
  497. weird ChIP primers! - (reply: 1)
  498. PCR primers - (reply: 1)
  499. diluting to final primer concentration - please help correct (reply: 1)
  500. Smear problem with my new primer set - (reply: 2)
  501. Amplifying a gene using a degenerate primer - (reply: 4)
  502. Making Primer Dimers on Purpose - odd I know...but humor me (reply: 5)
  503. Primer Design - Design Primer with DNA Sequence (reply: 1)
  504. PCR arrays=Primers in a plate? - Can any PCR mix be used? (reply: 1)
  505. Primer tm - (reply: 1)
  506. primer design - really necessary? (reply: 2)
  507. Degenerative primers = multiple products? - (reply: 5)
  508. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
  509. Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
  510. primer dimers - (reply: 5)
  511. qPCR primer design - (reply: 5)
  512. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
  513. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
  514. mouse Gapdh chip-qPCR primers for control! - (reply: 7)
  515. Primer stock confusion - (reply: 6)
  516. primer design - primer design question (reply: 2)
  517. Determine annealing temperature of primers - (reply: 14)
  518. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
  519. Validating real time pcr primers - (reply: 2)
  520. BSP,MSP primer design? - (reply: 1)
  521. low gc primers - help with pcr using low gc primers (reply: 6)
  522. Primers and annealing temperature - (reply: 2)
  523. WHich primer to use for sequencing - (reply: 5)
  524. primer design for pBAD topo expression kit - (reply: 2)
  525. primers fpr sequencing - (reply: 4)
  526. PCR with pfu and degenerate primers (?) - (reply: 7)
  527. Draw PCR primer locations - PCR primer (reply: 4)
  528. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
  529. Program for mapping primers to gene - diagram maker, visualize - (reply: 4)
  530. long PCR primer - (reply: 7)
  531. Taqman rtPCR primer and probe design - (reply: 3)
  532. Amplifying plasmid - non specific binding of primer - (reply: 3)
  533. primer design - (reply: 3)
  534. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
  535. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  536. design primer - when I design a primer is it necessary to include the restriction (reply: 1)
  537. Difference in Primer Melting Temperatures - Maximum allowable Tm difference? (reply: 4)
  538. Primers Not working - Real time PCR using SYBR Green (reply: 3)
  539. PCR conditions with three primers - (reply: 1)
  540. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  541. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
  542. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
  543. using the meth primer - (reply: 2)
  544. Generating primers for MSP - (reply: 1)
  545. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  546. Primer design and need help - (reply: 11)
  547. Programme for degenerate primer design - Programme for primer design (reply: 9)
  548. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  549. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)