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Primer Related Discussions
  1. Same primers and different melting peaks for two samples (~1C appart) - (reply: 2)
  2. Designing primers with his-tag for pET28a+ vector - (reply: 2)
  3. Can someone help me on my primer design? (contains flag tag) - (reply: 2)
  4. testing primers efficiency for rt-PCR - (reply: 1)
  5. Primer sequences with M and Y - how to order? - (reply: 2)
  6. need help calculating stock primer concentration - (reply: 1)
  7. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
  8. methylation specifc primers thermal cycle conditions - (reply: 4)
  9. PCR Amplification Issues and Primer dimer - (reply: 4)
  10. Primer calculation for qPCR - final reaction - (reply: 1)
  11. Sec. structure in primers - (reply: 1)
  12. PCR master mix containing primers and Taq - (reply: 4)
  13. Primer working with some cDNA samples and not other - (reply: 2)
  14. Primer dilution - (reply: 7)
  15. why not working msp primers - (reply: 4)
  16. Volume of Primers - (reply: 4)
  17. Primer Designing - (reply: 9)
  18. pcr primer design - (reply: 6)
  19. Please help (primer/ probe final volume calculations) - (reply: 1)
  20. Primer design with EcoRI ends - (reply: 2)
  21. PCR with overlapping primers only (primer extention) - (reply: 3)
  22. MSP Primer design - (reply: 5)
  23. How can I check the quality of bacterial primers and their specificity on blast? - (reply: 1)
  24. Primer design - (reply: 1)
  25. Primer non specific binding sites - (reply: 7)
  26. Degenerate primers - (reply: 8)
  27. Primers with 2 different annealing temp - (reply: 1)
  28. Y-linker transgene integration site PCR primer verification - (reply: 1)
  29. NEB TM CALCULATOR PRIMER CONCENTRATION - (reply: 2)
  30. primer design for a gene - (reply: 3)
  31. confirmation for primers dilution - (reply: 10)
  32. Possible primer dimer problem..Need solution!!! - (reply: 2)
  33. confusing with where to put primers and which ordination - (reply: 4)
  34. Help with primer concentration for sequencing - (reply: 1)
  35. Design the primers - (reply: 10)
  36. real time PCR primer design - (reply: 1)
  37. primers for cloning - (reply: 1)
  38. Degenerated Primers and their problems - (reply: 1)
  39. primer design with restriction enzyme - (reply: 1)
  40. Interpreting primer BLAST scores for self-complementary - (reply: 5)
  41. primer dilution help - (reply: 1)
  42. Testing primers on 'unknown' tissue - (reply: 1)
  43. could you help me with my stem loop RT primer? - (reply: 1)
  44. excess amount of primers - (reply: 2)
  45. Dehydrating and Reconstituting primers - (reply: 15)
  46. KIR gene promoters for MSP primers design - (reply: 2)
  47. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
  48. Primer as limiting reagent in PCR reaction - (reply: 2)
  49. Primer Design for RNA probes - (reply: 2)
  50. Nested BSP Primer Design - (reply: 6)
  51. website for primer design - (reply: 1)
  52. How to do a primer dilution - (reply: 10)
  53. Primer design and alternative transcripts - (reply: 2)
  54. NCBI Primer Design - Stringency Issues - (reply: 3)
  55. Different primer concentration in qPCR - (reply: 1)
  56. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
  57. Why when disigning primers for RTPCR, you need to chose a region where introns a - (reply: 1)
  58. Primer Specificity: Testing only one primer - (reply: 4)
  59. tool for comparing many primers pairs - (reply: 4)
  60. Pcr primers - (reply: 7)
  61. Primers have worked well but now getting primer dimers? - (reply: 2)
  62. I cannot design primers on exon-exon junction - (reply: 2)
  63. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  64. Designing primers in UTRs - (reply: 1)
  65. primer design@ buy? - (reply: 2)
  66. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
  67. designing primers( selecting target sequence/amplicon design) - (reply: 3)
  68. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  69. How to design primer to amplify genomic DNA? - (reply: 3)
  70. Primers - (reply: 1)
  71. Primers mix - (reply: 2)
  72. Having problem with primers for qPCR - (reply: 4)
  73. Troubleshooting methylation primers for Bio-Rad PCR - (reply: 3)
  74. No bands despite different primers and conditions and TAqs - (reply: 8)
  75. Equimolar Mix Primer - (reply: 8)
  76. Primer design - (reply: 5)
  77. storage for lyophilized primers - (reply: 1)
  78. Repeated mutagensis primer in site-directed mutagenesis - (reply: 8)
  79. PCR Primer trouble - (reply: 2)
  80. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
  81. finding the corrosponding primers - (reply: 2)
  82. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
  83. Designing primers for a nuclear region to amplify in a species that has no nucle - (reply: 1)
  84. T7 and M13 primers two band amplification - (reply: 3)
  85. fluorescent primer vs fluorescent terminator in sequencing - (reply: 4)
  86. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
  87. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
  88. Melting curve is irregular for primer optimization - (reply: 5)
  89. Designing primers for ABO blood groups - (reply: 1)
  90. RT-PCR primer design - (reply: 7)
  91. Is this primer okay? - (reply: 4)
  92. Can I use Primer as template? - (reply: 1)
  93. Resuspending primers calculation - (reply: 4)
  94. What do you use to check primer secondary structure? - (reply: 3)
  95. mutagenic primers with very high GC content. - (reply: 3)
  96. site-directed mutagenesis primer Tm - (reply: 4)
  97. Random vs oligo primer in preamplification RT - (reply: 1)
  98. Primer concentration - stupid question - (reply: 3)
  99. Primer Design, help im New - (reply: 4)
  100. RT-qPCR primer problem - (reply: 4)
  101. [Video] Using NCBI for RT-PCR Primer Design - (reply: 1)
  102. Designing cloning primers for DNMT - (reply: 2)
  103. Are these primer products good enough for qPCR? - (reply: 3)
  104. Can't get PCR with large overhang primers to work - (reply: 8)
  105. Primer Efficiency across runs - (reply: 1)
  106. Designing PCR Primers for cloning - (reply: 17)
  107. Creating primers to add restriction sites to vector backbone - (reply: 7)
  108. Some primers dose not work - (reply: 5)
  109. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
  110. Query regarding primers for quick change mutagenesis - (reply: 3)
  111. the storage time for primers - (reply: 9)
  112. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  113. random primers or oligodT - (reply: 4)
  114. To design or use published primers? - (reply: 4)
  115. PCR primer usage (Clonning & cDNA) - (reply: 2)
  116. problem in cloning PCR primer design with restriction site - (reply: 4)
  117. Amplification with U primers, but not with M primers - (reply: 1)
  118. His tag introduction in to gene and primer design - (reply: 2)
  119. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
  120. Design primer from incomplete sequence... - (reply: 2)
  121. Design primer from incomplete sequence... - (reply: 2)
  122. Primer designing for Methylation - (reply: 6)
  123. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
  124. Overlapping sequence PCR primers - (reply: 1)
  125. BSP PCR primer design explained - (reply: 11)
  126. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
  127. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
  128. good 16S univ. primer for qPCR? - (reply: 3)
  129. I need help for designing a pair of primers for this sequence. - (reply: 1)
  130. long primers PCR - (reply: 4)
  131. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
  132. Sequencing Primers and Plasmids - (reply: 3)
  133. Primers to amplify Kan operon from pet28 - (reply: 2)
  134. Tagged primers and a second PCR targetting the tags - (reply: 5)
  135. Barcode generator for PCR primers - (reply: 2)
  136. Biotinylated primers - (reply: 4)
  137. Transcription of PCR products without a primer coupled with promotor sequence - (reply: 4)
  138. How to join two sequencing files taken form forward and reverse primer - (reply: 3)
  139. Control primers for bisulfite-converted DNA - (reply: 3)
  140. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
  141. Is 27F and Anti-Gamma Primer the same thing for 16s? - (reply: 9)
  142. Q regarding primer mix for MSP!! - (reply: 4)
  143. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  144. BSP Primers with M13 Tails - (reply: 1)
  145. issues when designing PCR primers using PRIMER3 - (reply: 8)
  146. Primer catalog/databasing - (reply: 1)
  147. Primer Blast - (reply: 6)
  148. Help designing primers for use in In-fushion cloning - (reply: 4)
  149. Primers Freeze-Thaw - (reply: 16)
  150. primer design using 16s rRNA - (reply: 1)
  151. Primer concentration for cDNA synthesis using GSP - (reply: 1)
  152. Primer Design HELP! - (reply: 3)
  153. Bisulfite PCR doesn't work and the primer seems abnormal - (reply: 6)
  154. How are Primers made? - (reply: 9)
  155. designing methylation specific primers - (reply: 3)
  156. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  157. Primer Annealing Temperatures - (reply: 24)
  158. same primers, different product in conventional PCR and qPCR - (reply: 6)
  159. Different primer optimalization for nested vs direct MSP? - (reply: 3)
  160. Primers amplify genomic DNA but not cDNA - (reply: 2)
  161. Primer BLAST - (reply: 1)
  162. PCR primer design - published primers trustable? - (reply: 8)
  163. question about universal and specific primers for miRNA real time pcr - (reply: 1)
  164. Can we use degenerate bases in BSP primers? - (reply: 1)
  165. How to check primer sequences using BLAST and other tools? - (reply: 2)
  166. Primer Check? - (reply: 2)
  167. Validation of PCR primers/ probes - (reply: 6)
  168. Two reverse primer sequences for a single forward primer - (reply: 1)
  169. PCR with more than one primer - (reply: 1)
  170. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  171. RT-PCR primer design - (reply: 1)
  172. No or very weak band using 16s primers - (reply: 1)
  173. No PCR amplification with b-actin primers - (reply: 1)
  174. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
  175. Primer Design Help: GFP primers for Arabidopsis - (reply: 1)
  176. No PCR amplified with long primers - (reply: 10)
  177. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  178. another primer dilution question - (reply: 2)
  179. Primer Efficiency - (reply: 2)
  180. oligo(dt) 15 vs random primers - (reply: 3)
  181. primer dilutions - (reply: 4)
  182. Genotyping PCR primers - (reply: 1)
  183. MSP Primers not working - (reply: 6)
  184. Primers too dilute - (reply: 1)
  185. Cloning HELP- Possible Primer issues - (reply: 6)
  186. PCR primer pairs - university exam - (reply: 7)
  187. Help with primer design through PRIMER3 - (reply: 4)
  188. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  189. Tm of primers - prediction and verification - (reply: 1)
  190. how to design primers for 16sr RNA - (reply: 24)
  191. Help with primer design - (reply: 3)
  192. Primer Tm calculation. - (reply: 1)
  193. Running Primers on Agarose Gel - (reply: 6)
  194. PCR product now not getting with same primers and other PCR conditions - (reply: 4)
  195. universal primers for PCR ribotyping - (reply: 6)
  196. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  197. Primer design: Tm above or below Ta? - (reply: 5)
  198. Oligo primers for shRNA construction - (reply: 1)
  199. Design primers for expression cloning - (reply: 11)
  200. primer dilution query - (reply: 2)
  201. Using BLAST to check primers - (reply: 1)
  202. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  203. What is the function of a third primer in a PCR mix?? - (reply: 2)
  204. How can we design a primer ? step by step method - (reply: 4)
  205. EPIGENETICS-NEWBIE-Weird/too close primers for pyrosequencing assay - (reply: 2)
  206. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
  207. Is it possible to amplify cDNA with hexamer primers or oligo dT primers to have - (reply: 1)
  208. annealing temperature for 3 primer pairs? - (reply: 4)
  209. Impact of primer sequence on TA cloning - (reply: 3)
  210. Pcr primers - (reply: 2)
  211. RT-Q PCR primer design question - (reply: 2)
  212. multiplex rtqPCR and custom primer/probe design - (reply: 2)
  213. big differnece in my primers Tm - (reply: 2)
  214. Designing primers for the ORFs - (reply: 9)
  215. PCR with phophorylated primers - (reply: 1)
  216. Problem with New qPCR primers - help! - (reply: 6)
  217. Designing primers for miRNA located on minus strand. - (reply: 2)
  218. Plasmid sequencing without primers - (reply: 1)
  219. RT-PCR primers and the poly(A) tail - (reply: 2)
  220. overlapping primer - (reply: 1)
  221. PCR primers help - (reply: 5)
  222. Primer dimers - (reply: 2)
  223. Primer validation and PCR efficiency - (reply: 4)
  224. should I design primers spanning both intron and exon - (reply: 2)
  225. PCR with a very long and a short primer - (reply: 5)
  226. Comparison between two primers pairs? Is it possible? - (reply: 3)
  227. Rt-PCR primer design - (reply: 7)
  228. Length of non-binding sequence in primers with RE site - (reply: 6)
  229. Orientation of primers - (reply: 2)
  230. methylation analysis with MS-HRM (primer design) - (reply: 1)
  231. primer resuspension - (reply: 2)
  232. Help designing primers with restriction sites - (reply: 3)
  233. Why primers shouldn't have Tm difference greater than 5C? - (reply: 5)
  234. PCR primer with no extra DNA end - (reply: 5)
  235. Tm calculation for primers with RE sites and overhangs - (reply: 4)
  236. Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
  237. checking msp primers - (reply: 2)
  238. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  239. Another primer dimers problem - (reply: 23)
  240. MSP U primers very difficult to amplify... - (reply: 4)
  241. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
  242. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  243. Primer design - (reply: 3)
  244. single primer PCR - (reply: 5)
  245. Primer concentration - (reply: 4)
  246. Primer 3' mismatch - Strange experience (reply: 9)
  247. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
  248. PRIMER DILUTION - STOCK SOLUTION TO A WORKING STANDARD (reply: 2)
  249. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
  250. Are my MSP primers good? - MSP primers design by Methyl Primer Express v1.0 (reply: 2)
  251. Primer dilution --> problem.. - (reply: 16)
  252. Sybr green RT-qPCR primer - (reply: 1)
  253. Sybr green RT-qPCR primer - (reply: 1)
  254. primer design - microalgae (reply: 2)
  255. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
  256. how to overcome primer contamination - need protocol (reply: 11)
  257. RT-PCR primer design - Primer design and evaluation (reply: 7)
  258. STANDARDISATION OF MULTIPLEX PCR - IN order to standardise a multiplex PCR for 3 set of primers (reply: 3)
  259. Primer,probes dilution - (reply: 3)
  260. PCR: Cloning Primers - (reply: 8)
  261. Exon Spanning PCR Primers - (reply: 1)
  262. Primer sequences - (reply: 1)
  263. PCR Primers - (reply: 2)
  264. How to blast methylation specific and unspecific primers - (reply: 1)
  265. How to check primers are of correct sequence or not? - (reply: 2)
  266. Primers... - (reply: 1)
  267. When are primer dimers a problem? - (reply: 1)
  268. primer with higher melting temperature - (reply: 2)
  269. Problems designing primers for BSP - (reply: 2)
  270. qPCR primer design - possible off-target (reply: 1)
  271. Primer dimer formation and real-time PCR - (reply: 7)
  272. Primer Design Urgent! - (reply: 1)
  273. how to know my primer's sense ??? - help ! (reply: 2)
  274. intron spanning primers for non model organisms - (reply: 2)
  275. Primer design - (reply: 2)
  276. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
  277. Primer seq - (reply: 2)
  278. oligo dt and random primer - (reply: 3)
  279. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
  280. Primers and self and hetero dimers - (reply: 1)
  281. Primers for qPCR - (reply: 3)
  282. RT-PCR primer - (reply: 3)
  283. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
  284. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  285. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  286. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  287. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  288. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  289. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
  290. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
  291. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
  292. ChIP primer design - (reply: 1)
  293. Bisulfite sequencing primer design query (probably simple) - (reply: 4)
  294. Question about Primers - (reply: 3)
  295. Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
  296. designing MSP primers (dimers NOOOOOOOO!) - (reply: 1)
  297. Primer design with a tag - (reply: 4)
  298. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
  299. Combining forward and reverse primer gives different size on gel - (reply: 2)
  300. primers for fungal identification - (reply: 9)
  301. difference between PCR primers and sequencing primers - (reply: 1)
  302. Sense and antisense DNA, and primer design - (reply: 6)
  303. Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
  304. Question about RealTime PCR Primer Design - (reply: 1)
  305. Designing a Primer with a RS that has a W - (reply: 1)
  306. Primers stopped working!? - PCR Primers (reply: 6)
  307. NFK beta primers - (reply: 4)
  308. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
  309. Primer design - Primer design (reply: 2)
  310. Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
  311. Real Time PCR Primers and Probes - (reply: 2)
  312. Need help - How we can design primer for miRNA (reply: 1)
  313. primer and promoter sequences - (reply: 1)
  314. designing of primers - (reply: 2)
  315. Primer Tm differences/ PCR - (reply: 1)
  316. Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
  317. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
  318. d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
  319. Designing primer to remove his-tag - (reply: 1)
  320. PCR Primer Dilution from F+R - (reply: 1)
  321. primer reconstitution - primer reconstitution (reply: 4)
  322. Primer design for insert amplification - Is this correct? (reply: 2)
  323. HRM primers - (reply: 1)
  324. primer design - (reply: 2)
  325. New to sequencing, primer design - (reply: 2)
  326. Quickchange mutagenesis primer - (reply: 3)
  327. pcr / primer theory in bisulfite sequencing - (reply: 4)
  328. Primer Design..need very basic advice. - Please help, I cant figure this out for the life of me. (reply: 2)
  329. Problems with the Specificity of the Primers - (reply: 2)
  330. confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
  331. Degenerate primers PCR problem, Please help! - (reply: 2)
  332. Primer design help needed - (reply: 1)
  333. Primers no longer work - (reply: 1)
  334. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
  335. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
  336. Calculating Primer concentrations for PCR - Is there an easy way to do this...help (reply: 3)
  337. primer Tm is too high, how tu get pcr product - help! (reply: 21)
  338. Find universal primers in vector - any online tool? (reply: 6)
  339. Picking primers to confirm Illumina meth27 results - (reply: 2)
  340. Colony Pcr - primers - (reply: 5)
  341. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
  342. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  343. Primer Dilution Problem - D'oh! (reply: 2)
  344. Is there any software that can be sued to design degenerate primers - (reply: 1)
  345. Primer Decontamination - (reply: 2)
  346. tools for checking non-specific binding of primers -
    (reply: 1)
  347. Primer designed for ARMS PCR - (reply: 2)
  348. methylation specific PCR - Primers - (reply: 1)
  349. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
  350. Primer Design - aligning sequences for probe/primer design (reply: 2)
  351. PCR with one primer - (reply: 1)
  352. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
  353. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
  354. High Tm on primers - cannot get a product - (reply: 8)
  355. NCBI primer blast problem - (reply: 1)
  356. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
  357. primer sequence problem - (reply: 2)
  358. Finding bisulfite PCR primer sequence - (reply: 3)
  359. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
  360. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
  361. How to design primers to check my candidates in a ChiP assay - (reply: 4)
  362. Primer Reconstitution--does temperature matter? - (reply: 5)
  363. Primer Design MSP, BSP, MS-HRM - (reply: 3)
  364. Primers that do not align at regions with SNPs. Where to find SNPs? - (reply: 2)
  365. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  366. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
  367. Primer design for qPCR - (reply: 2)
  368. Primer design for qPCR - (reply: 1)
  369. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
  370. primer design tips - (reply: 3)
  371. design pcr primer - (reply: 1)
  372. PCR with long and complex primers - (reply: 2)
  373. Phospholylation of primers - (reply: 1)
  374. LINE1 OFR2 Primer Design - (reply: 1)
  375. phosphorylation of primers using PNK - (reply: 1)
  376. Primers for yeast (18S) - (reply: 1)
  377. What's the longest overhanging primer seq. you have used? - (reply: 2)
  378. Primer Concentration to lower Ct - (reply: 1)
  379. Primer Concentration Help - (reply: 4)
  380. Primer Degradation - What exactly happens? (reply: 1)
  381. Primers in 2 exons - (reply: 2)
  382. Problems with designing a primer - (reply: 2)
  383. Probe or Primer info for bovine AQP-1 - (reply: 6)
  384. Different sized product for same BS primer! - (reply: 2)
  385. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  386. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
  387. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  388. primers with restriction site - (reply: 1)
  389. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
  390. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
  391. primer design by generunner DG - (reply: 1)
  392. BSP primers design, help please - (reply: 2)
  393. primers with fluoresent dye PCR cycle question - (reply: 4)
  394. Urgent! Sequencing a mixture of ssDNA fragments of the same gene - ssDNA different in size amplified from a single primer (reply: 2)
  395. Problems with validating primers and low expression genes - (reply: 3)
  396. intron/exon spanning primers - (reply: 1)
  397. MSP primer troubles - (reply: 3)
  398. Designing Primers for multiple Isoforms - (reply: 1)
  399. real time pcr melt curve and primer efficiency problem - (reply: 3)
  400. how to design primer for my n-terminal sequence - hot to design primer for my protein (reply: 2)
  401. Addition of Restriction sites into PCR primers - (reply: 4)
  402. Primer Design in 3non translated version vs. coding region - (reply: 2)
  403. different PCR primers for real-time and classic PCR - (reply: 3)
  404. primers deconamination??? - (reply: 6)
  405. primer check!!! - (reply: 6)
  406. Annealing Temperature of biotinylated primers - (reply: 2)
  407. primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
  408. Primer clean-up - ExoSAP-It isn't working (reply: 6)
  409. Primer dilution - PCR primer dilution (reply: 2)
  410. Long Primers for PCR - (reply: 2)
  411. Primers going "loopy" - (reply: 2)
  412. Conversion of primer unit - ug/ul to uM (reply: 4)
  413. Primer Trouble Shooting - (reply: 5)
  414. Proven Primers Stopped working & now leave smear! - (reply: 2)
  415. a simple primer problem - (reply: 5)
  416. Primers from 3'UTR - (reply: 2)
  417. Designing primers with ESTs - (reply: 2)
  418. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
  419. addition of BclI restriction site to PCR primer - (reply: 6)
  420. How does methprimer calculate primer Tm - (reply: 2)
  421. Phosphorylated primers - (reply: 5)
  422. QPCR - Primer and Probe question - QPCR (reply: 2)
  423. Easy primer question? - Primers (reply: 1)
  424. primer dimer - proplems (reply: 1)
  425. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
  426. Primer efficiency test - (reply: 1)
  427. primer software - (reply: 2)
  428. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
  429. How to know in which exon a primer match? - (reply: 1)
  430. primer annealing - higher annealing= less wrong bounds (reply: 4)
  431. sequencing with forward/reverse primers - (reply: 7)
  432. Adding A overhangs - primer design implications? - (reply: 1)
  433. primer RE over-hang nucleotides - common sequences? (reply: 5)
  434. Forward and reverse primers got very different Tm - what to do? (reply: 11)
  435. primer design problem - (reply: 1)
  436. Problem with primer efficiency analysis - (reply: 4)
  437. primer design - (reply: 1)
  438. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
  439. designing primers for genes not sequenced yet - (reply: 2)
  440. Primer desing - (reply: 2)
  441. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
  442. primer design - (reply: 9)
  443. primer contamination - primer contaminated with ice (reply: 3)
  444. Primer3 vs Primer BLAST - (reply: 3)
  445. Primer design: free energy - (reply: 2)
  446. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  447. Primer optimization for ChIP - (reply: 2)
  448. sequencing primer: T7 or T7promoter? - (reply: 4)
  449. primer tm - tm calculation for tagged primers (reply: 2)
  450. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
  451. Universal 16s rRNA primers needed - (reply: 3)
  452. PCR primers for histone mod ChIP - How to find the regions (reply: 1)
  453. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
  454. adding koazak sequence to primer - (reply: 1)
  455. Designing primers for cloning - After primer designing, how should I perform the PCR? (reply: 15)
  456. Primer design if sequence is unknown for organism - (reply: 5)
  457. adding C terminal tag to reverse primer - (reply: 1)
  458. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
  459. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
  460. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
  461. what primers should I use for DNA sequencing? please help - (reply: 2)
  462. what primers should I use for DNA sequencing?? - (reply: 3)
  463. Is my primer going to work for qRT-PCR? - (reply: 6)
  464. Primers annealing temperatures - (reply: 8)
  465. Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
  466. Synthesize own Oligo-dT primers - (reply: 6)
  467. Testing primer specificity... - ...without gDNA of target organisms (reply: 6)
  468. primer design - Primer design for PCR (reply: 1)
  469. How are primers designed? - (reply: 4)
  470. need help on primer calcuation soon - (reply: 1)
  471. primer design - (reply: 6)
  472. 23S rRNA Primer - need primer sequence (reply: 1)
  473. Freezing primers+SYBR green - (reply: 2)
  474. Tagged Primers - (reply: 5)
  475. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
  476. design primer for chip - (reply: 5)
  477. How to quantify oligonucleotide primers using nanodrop ND-1000 software - newbie help (reply: 2)
  478. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  479. reconstitution of primers - (reply: 2)
  480. primer dimer - (reply: 2)
  481. PCR with long primers - (reply: 3)
  482. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  483. Help me! How to design nested BSP primers? - (reply: 4)
  484. Using BLAST to check primers - (reply: 17)
  485. PCR primer - (reply: 2)
  486. Primer design - GC clamp - (reply: 8)
  487. primer dimer or what? - (reply: 2)
  488. Primers and Taqman Probes mixture question - (reply: 1)
  489. designing primers - (reply: 1)
  490. Negative Control Primers for ChIP Assay - (reply: 3)
  491. PCR Cloning-large primers - (reply: 4)
  492. forward and reverse primer - (reply: 4)
  493. Check the primers - (reply: 4)
  494. BSP primers - (reply: 2)
  495. Primer dimer issue in real time PCR - (reply: 21)
  496. RT-PCR primer dimers and cDNA degradation - (reply: 2)
  497. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  498. primer binding - (reply: 1)
  499. weird ChIP primers! - (reply: 1)
  500. PCR primers - (reply: 1)
  501. diluting to final primer concentration - please help correct (reply: 1)
  502. Smear problem with my new primer set - (reply: 2)
  503. Amplifying a gene using a degenerate primer - (reply: 4)
  504. Making Primer Dimers on Purpose - odd I know...but humor me (reply: 5)
  505. Primer Design - Design Primer with DNA Sequence (reply: 1)
  506. PCR arrays=Primers in a plate? - Can any PCR mix be used? (reply: 1)
  507. Primer tm - (reply: 1)
  508. primer design - really necessary? (reply: 2)
  509. Degenerative primers = multiple products? - (reply: 5)
  510. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
  511. Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
  512. primer dimers - (reply: 5)
  513. qPCR primer design - (reply: 5)
  514. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
  515. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
  516. mouse Gapdh chip-qPCR primers for control! - (reply: 7)
  517. Primer stock confusion - (reply: 6)
  518. primer design - primer design question (reply: 2)
  519. Determine annealing temperature of primers - (reply: 14)
  520. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
  521. Validating real time pcr primers - (reply: 2)
  522. BSP,MSP primer design? - (reply: 1)
  523. low gc primers - help with pcr using low gc primers (reply: 6)
  524. Primers and annealing temperature - (reply: 2)
  525. WHich primer to use for sequencing - (reply: 5)
  526. primer design for pBAD topo expression kit - (reply: 2)
  527. primers fpr sequencing - (reply: 4)
  528. PCR with pfu and degenerate primers (?) - (reply: 7)
  529. Draw PCR primer locations - PCR primer (reply: 4)
  530. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
  531. Program for mapping primers to gene - diagram maker, visualize - (reply: 4)
  532. long PCR primer - (reply: 7)
  533. Taqman rtPCR primer and probe design - (reply: 3)
  534. Amplifying plasmid - non specific binding of primer - (reply: 3)
  535. primer design - (reply: 3)
  536. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
  537. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  538. design primer - when I design a primer is it necessary to include the restriction (reply: 1)
  539. Difference in Primer Melting Temperatures - Maximum allowable Tm difference? (reply: 4)
  540. Primers Not working - Real time PCR using SYBR Green (reply: 3)
  541. PCR conditions with three primers - (reply: 1)
  542. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  543. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
  544. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
  545. using the meth primer - (reply: 2)
  546. Generating primers for MSP - (reply: 1)
  547. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  548. Primer design and need help - (reply: 11)
  549. Programme for degenerate primer design - Programme for primer design (reply: 9)
  550. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  551. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)