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Primer Related Discussions
  1. NEB TM CALCULATOR PRIMER CONCENTRATION - (reply: 2)
  2. primer design for a gene - (reply: 2)
  3. confirmation for primers dilution - (reply: 10)
  4. Possible primer dimer problem..Need solution!!! - (reply: 2)
  5. confusing with where to put primers and which ordination - (reply: 4)
  6. Help with primer concentration for sequencing - (reply: 1)
  7. Design the primers - (reply: 10)
  8. real time PCR primer design - (reply: 1)
  9. primers for cloning - (reply: 1)
  10. Degenerated Primers and their problems - (reply: 1)
  11. primer design with restriction enzyme - (reply: 1)
  12. Interpreting primer BLAST scores for self-complementary - (reply: 5)
  13. primer dilution help - (reply: 1)
  14. Testing primers on 'unknown' tissue - (reply: 1)
  15. could you help me with my stem loop RT primer? - (reply: 1)
  16. excess amount of primers - (reply: 2)
  17. Dehydrating and Reconstituting primers - (reply: 15)
  18. KIR gene promoters for MSP primers design - (reply: 2)
  19. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
  20. Primer as limiting reagent in PCR reaction - (reply: 2)
  21. Primer Design for RNA probes - (reply: 2)
  22. Nested BSP Primer Design - (reply: 6)
  23. website for primer design - (reply: 1)
  24. How to do a primer dilution - (reply: 10)
  25. Primer design and alternative transcripts - (reply: 2)
  26. NCBI Primer Design - Stringency Issues - (reply: 3)
  27. Different primer concentration in qPCR - (reply: 1)
  28. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
  29. Why when disigning primers for RTPCR, you need to chose a region where introns a - (reply: 1)
  30. Primer Specificity: Testing only one primer - (reply: 4)
  31. tool for comparing many primers pairs - (reply: 4)
  32. Pcr primers - (reply: 7)
  33. Primers have worked well but now getting primer dimers? - (reply: 2)
  34. I cannot design primers on exon-exon junction - (reply: 2)
  35. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  36. Designing primers in UTRs - (reply: 1)
  37. primer design@ buy? - (reply: 2)
  38. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
  39. designing primers( selecting target sequence/amplicon design) - (reply: 3)
  40. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  41. How to design primer to amplify genomic DNA? - (reply: 3)
  42. Primers - (reply: 1)
  43. Primers mix - (reply: 2)
  44. Having problem with primers for qPCR - (reply: 4)
  45. Troubleshooting methylation primers for Bio-Rad PCR - (reply: 3)
  46. No bands despite different primers and conditions and TAqs - (reply: 8)
  47. Equimolar Mix Primer - (reply: 8)
  48. Primer design - (reply: 5)
  49. storage for lyophilized primers - (reply: 1)
  50. Repeated mutagensis primer in site-directed mutagenesis - (reply: 8)
  51. PCR Primer trouble - (reply: 2)
  52. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
  53. finding the corrosponding primers - (reply: 2)
  54. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
  55. Designing primers for a nuclear region to amplify in a species that has no nucle - (reply: 1)
  56. T7 and M13 primers two band amplification - (reply: 3)
  57. fluorescent primer vs fluorescent terminator in sequencing - (reply: 4)
  58. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
  59. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
  60. Melting curve is irregular for primer optimization - (reply: 5)
  61. Designing primers for ABO blood groups - (reply: 1)
  62. RT-PCR primer design - (reply: 7)
  63. Is this primer okay? - (reply: 4)
  64. Resuspending primers calculation - (reply: 4)
  65. What do you use to check primer secondary structure? - (reply: 3)
  66. mutagenic primers with very high GC content. - (reply: 3)
  67. site-directed mutagenesis primer Tm - (reply: 4)
  68. Random vs oligo primer in preamplification RT - (reply: 1)
  69. Primer concentration - stupid question - (reply: 3)
  70. Primer Design, help im New - (reply: 4)
  71. RT-qPCR primer problem - (reply: 4)
  72. [Video] Using NCBI for RT-PCR Primer Design - (reply: 1)
  73. Designing cloning primers for DNMT - (reply: 2)
  74. Are these primer products good enough for qPCR? - (reply: 3)
  75. Can't get PCR with large overhang primers to work - (reply: 8)
  76. Primer Efficiency across runs - (reply: 1)
  77. Designing PCR Primers for cloning - (reply: 17)
  78. Creating primers to add restriction sites to vector backbone - (reply: 7)
  79. Some primers dose not work - (reply: 5)
  80. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
  81. Query regarding primers for quick change mutagenesis - (reply: 3)
  82. the storage time for primers - (reply: 9)
  83. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  84. random primers or oligodT - (reply: 4)
  85. To design or use published primers? - (reply: 4)
  86. PCR primer usage (Clonning & cDNA) - (reply: 2)
  87. problem in cloning PCR primer design with restriction site - (reply: 4)
  88. Amplification with U primers, but not with M primers - (reply: 1)
  89. His tag introduction in to gene and primer design - (reply: 2)
  90. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
  91. Design primer from incomplete sequence... - (reply: 2)
  92. Design primer from incomplete sequence... - (reply: 2)
  93. Primer designing for Methylation - (reply: 6)
  94. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
  95. Overlapping sequence PCR primers - (reply: 1)
  96. BSP PCR primer design explained - (reply: 11)
  97. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
  98. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
  99. good 16S univ. primer for qPCR? - (reply: 3)
  100. I need help for designing a pair of primers for this sequence. - (reply: 1)
  101. long primers PCR - (reply: 4)
  102. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
  103. Sequencing Primers and Plasmids - (reply: 3)
  104. Primers to amplify Kan operon from pet28 - (reply: 2)
  105. Tagged primers and a second PCR targetting the tags - (reply: 5)
  106. Barcode generator for PCR primers - (reply: 2)
  107. Biotinylated primers - (reply: 4)
  108. Transcription of PCR products without a primer coupled with promotor sequence - (reply: 4)
  109. How to join two sequencing files taken form forward and reverse primer - (reply: 3)
  110. Control primers for bisulfite-converted DNA - (reply: 3)
  111. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
  112. Is 27F and Anti-Gamma Primer the same thing for 16s? - (reply: 9)
  113. Q regarding primer mix for MSP!! - (reply: 4)
  114. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  115. BSP Primers with M13 Tails - (reply: 1)
  116. issues when designing PCR primers using PRIMER3 - (reply: 8)
  117. Primer catalog/databasing - (reply: 1)
  118. Primer Blast - (reply: 6)
  119. Help designing primers for use in In-fushion cloning - (reply: 4)
  120. Primers Freeze-Thaw - (reply: 16)
  121. primer design using 16s rRNA - (reply: 1)
  122. Primer concentration for cDNA synthesis using GSP - (reply: 1)
  123. Primer Design HELP! - (reply: 3)
  124. Bisulfite PCR doesn't work and the primer seems abnormal - (reply: 6)
  125. How are Primers made? - (reply: 9)
  126. designing methylation specific primers - (reply: 3)
  127. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  128. Primer Annealing Temperatures - (reply: 24)
  129. same primers, different product in conventional PCR and qPCR - (reply: 6)
  130. Different primer optimalization for nested vs direct MSP? - (reply: 3)
  131. Primers amplify genomic DNA but not cDNA - (reply: 2)
  132. Primer BLAST - (reply: 1)
  133. PCR primer design - published primers trustable? - (reply: 8)
  134. question about universal and specific primers for miRNA real time pcr - (reply: 1)
  135. Can we use degenerate bases in BSP primers? - (reply: 1)
  136. How to check primer sequences using BLAST and other tools? - (reply: 2)
  137. Primer Check? - (reply: 2)
  138. Validation of PCR primers/ probes - (reply: 6)
  139. Two reverse primer sequences for a single forward primer - (reply: 1)
  140. PCR with more than one primer - (reply: 1)
  141. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  142. RT-PCR primer design - (reply: 1)
  143. No or very weak band using 16s primers - (reply: 1)
  144. No PCR amplification with b-actin primers - (reply: 1)
  145. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
  146. Primer Design Help: GFP primers for Arabidopsis - (reply: 1)
  147. No PCR amplified with long primers - (reply: 10)
  148. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  149. another primer dilution question - (reply: 2)
  150. Primer Efficiency - (reply: 2)
  151. oligo(dt) 15 vs random primers - (reply: 3)
  152. primer dilutions - (reply: 4)
  153. Genotyping PCR primers - (reply: 1)
  154. MSP Primers not working - (reply: 6)
  155. Primers too dilute - (reply: 1)
  156. Cloning HELP- Possible Primer issues - (reply: 6)
  157. PCR primer pairs - university exam - (reply: 7)
  158. Help with primer design through PRIMER3 - (reply: 4)
  159. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  160. Tm of primers - prediction and verification - (reply: 1)
  161. how to design primers for 16sr RNA - (reply: 24)
  162. Help with primer design - (reply: 3)
  163. Primer Tm calculation. - (reply: 1)
  164. Running Primers on Agarose Gel - (reply: 6)
  165. PCR product now not getting with same primers and other PCR conditions - (reply: 4)
  166. universal primers for PCR ribotyping - (reply: 6)
  167. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  168. Primer design: Tm above or below Ta? - (reply: 5)
  169. Oligo primers for shRNA construction - (reply: 1)
  170. Design primers for expression cloning - (reply: 11)
  171. primer dilution query - (reply: 2)
  172. Using BLAST to check primers - (reply: 1)
  173. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  174. What is the function of a third primer in a PCR mix?? - (reply: 2)
  175. How can we design a primer ? step by step method - (reply: 4)
  176. EPIGENETICS-NEWBIE-Weird/too close primers for pyrosequencing assay - (reply: 2)
  177. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
  178. Is it possible to amplify cDNA with hexamer primers or oligo dT primers to have - (reply: 1)
  179. annealing temperature for 3 primer pairs? - (reply: 4)
  180. Impact of primer sequence on TA cloning - (reply: 3)
  181. Pcr primers - (reply: 2)
  182. RT-Q PCR primer design question - (reply: 2)
  183. multiplex rtqPCR and custom primer/probe design - (reply: 2)
  184. big differnece in my primers Tm - (reply: 2)
  185. Designing primers for the ORFs - (reply: 9)
  186. PCR with phophorylated primers - (reply: 1)
  187. Problem with New qPCR primers - help! - (reply: 6)
  188. Designing primers for miRNA located on minus strand. - (reply: 2)
  189. Plasmid sequencing without primers - (reply: 1)
  190. RT-PCR primers and the poly(A) tail - (reply: 2)
  191. overlapping primer - (reply: 1)
  192. PCR primers help - (reply: 5)
  193. Primer dimers - (reply: 2)
  194. Primer validation and PCR efficiency - (reply: 4)
  195. should I design primers spanning both intron and exon - (reply: 2)
  196. PCR with a very long and a short primer - (reply: 5)
  197. Comparison between two primers pairs? Is it possible? - (reply: 3)
  198. Rt-PCR primer design - (reply: 7)
  199. Length of non-binding sequence in primers with RE site - (reply: 6)
  200. Orientation of primers - (reply: 2)
  201. methylation analysis with MS-HRM (primer design) - (reply: 1)
  202. primer resuspension - (reply: 2)
  203. Help designing primers with restriction sites - (reply: 3)
  204. Why primers shouldn't have Tm difference greater than 5C? - (reply: 5)
  205. PCR primer with no extra DNA end - (reply: 5)
  206. Tm calculation for primers with RE sites and overhangs - (reply: 4)
  207. Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
  208. checking msp primers - (reply: 2)
  209. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  210. Another primer dimers problem - (reply: 23)
  211. MSP U primers very difficult to amplify... - (reply: 4)
  212. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
  213. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  214. Primer design - (reply: 3)
  215. single primer PCR - (reply: 5)
  216. Primer concentration - (reply: 4)
  217. Primer 3' mismatch - Strange experience (reply: 9)
  218. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
  219. PRIMER DILUTION - STOCK SOLUTION TO A WORKING STANDARD (reply: 2)
  220. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
  221. Are my MSP primers good? - MSP primers design by Methyl Primer Express v1.0 (reply: 2)
  222. Primer dilution --> problem.. - (reply: 16)
  223. Sybr green RT-qPCR primer - (reply: 1)
  224. Sybr green RT-qPCR primer - (reply: 1)
  225. primer design - microalgae (reply: 2)
  226. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
  227. how to overcome primer contamination - need protocol (reply: 11)
  228. RT-PCR primer design - Primer design and evaluation (reply: 7)
  229. STANDARDISATION OF MULTIPLEX PCR - IN order to standardise a multiplex PCR for 3 set of primers (reply: 3)
  230. Primer,probes dilution - (reply: 3)
  231. PCR: Cloning Primers - (reply: 8)
  232. Exon Spanning PCR Primers - (reply: 1)
  233. Primer sequences - (reply: 1)
  234. PCR Primers - (reply: 2)
  235. How to blast methylation specific and unspecific primers - (reply: 1)
  236. How to check primers are of correct sequence or not? - (reply: 2)
  237. Primers... - (reply: 1)
  238. When are primer dimers a problem? - (reply: 1)
  239. primer with higher melting temperature - (reply: 2)
  240. Problems designing primers for BSP - (reply: 2)
  241. qPCR primer design - possible off-target (reply: 1)
  242. Primer dimer formation and real-time PCR - (reply: 7)
  243. Primer Design Urgent! - (reply: 1)
  244. how to know my primer's sense ??? - help ! (reply: 2)
  245. intron spanning primers for non model organisms - (reply: 2)
  246. Primer design - (reply: 2)
  247. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
  248. Primer seq - (reply: 2)
  249. oligo dt and random primer - (reply: 3)
  250. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
  251. Primers and self and hetero dimers - (reply: 1)
  252. Primers for qPCR - (reply: 3)
  253. RT-PCR primer - (reply: 3)
  254. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
  255. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  256. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  257. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  258. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  259. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  260. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
  261. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
  262. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
  263. ChIP primer design - (reply: 1)
  264. Bisulfite sequencing primer design query (probably simple) - (reply: 4)
  265. Question about Primers - (reply: 3)
  266. Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
  267. designing MSP primers (dimers NOOOOOOOO!) - (reply: 1)
  268. Primer design with a tag - (reply: 4)
  269. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
  270. Combining forward and reverse primer gives different size on gel - (reply: 2)
  271. primers for fungal identification - (reply: 9)
  272. difference between PCR primers and sequencing primers - (reply: 1)
  273. Sense and antisense DNA, and primer design - (reply: 6)
  274. Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
  275. Question about RealTime PCR Primer Design - (reply: 1)
  276. Designing a Primer with a RS that has a W - (reply: 1)
  277. Primers stopped working!? - PCR Primers (reply: 6)
  278. NFK beta primers - (reply: 4)
  279. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
  280. Primer design - Primer design (reply: 2)
  281. Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
  282. Real Time PCR Primers and Probes - (reply: 2)
  283. Need help - How we can design primer for miRNA (reply: 1)
  284. primer and promoter sequences - (reply: 1)
  285. designing of primers - (reply: 2)
  286. Primer Tm differences/ PCR - (reply: 1)
  287. Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
  288. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
  289. d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
  290. Designing primer to remove his-tag - (reply: 1)
  291. PCR Primer Dilution from F+R - (reply: 1)
  292. primer reconstitution - primer reconstitution (reply: 4)
  293. Primer design for insert amplification - Is this correct? (reply: 2)
  294. HRM primers - (reply: 1)
  295. primer design - (reply: 2)
  296. New to sequencing, primer design - (reply: 2)
  297. Quickchange mutagenesis primer - (reply: 3)
  298. pcr / primer theory in bisulfite sequencing - (reply: 4)
  299. Primer Design..need very basic advice. - Please help, I cant figure this out for the life of me. (reply: 2)
  300. Problems with the Specificity of the Primers - (reply: 2)
  301. confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
  302. Degenerate primers PCR problem, Please help! - (reply: 2)
  303. Primer design help needed - (reply: 1)
  304. Primers no longer work - (reply: 1)
  305. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
  306. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
  307. Calculating Primer concentrations for PCR - Is there an easy way to do this...help (reply: 3)
  308. primer Tm is too high, how tu get pcr product - help! (reply: 21)
  309. Find universal primers in vector - any online tool? (reply: 6)
  310. Picking primers to confirm Illumina meth27 results - (reply: 2)
  311. Colony Pcr - primers - (reply: 5)
  312. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
  313. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  314. Primer Dilution Problem - D'oh! (reply: 2)
  315. Is there any software that can be sued to design degenerate primers - (reply: 1)
  316. Primer Decontamination - (reply: 2)
  317. tools for checking non-specific binding of primers -
    (reply: 1)
  318. Primer designed for ARMS PCR - (reply: 2)
  319. methylation specific PCR - Primers - (reply: 1)
  320. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
  321. Primer Design - aligning sequences for probe/primer design (reply: 2)
  322. PCR with one primer - (reply: 1)
  323. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
  324. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
  325. High Tm on primers - cannot get a product - (reply: 8)
  326. NCBI primer blast problem - (reply: 1)
  327. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
  328. primer sequence problem - (reply: 2)
  329. Finding bisulfite PCR primer sequence - (reply: 3)
  330. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
  331. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
  332. How to design primers to check my candidates in a ChiP assay - (reply: 4)
  333. Primer Reconstitution--does temperature matter? - (reply: 5)
  334. Primer Design MSP, BSP, MS-HRM - (reply: 3)
  335. Primers that do not align at regions with SNPs. Where to find SNPs? - (reply: 2)
  336. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  337. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
  338. Primer design for qPCR - (reply: 2)
  339. Primer design for qPCR - (reply: 1)
  340. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
  341. primer design tips - (reply: 3)
  342. design pcr primer - (reply: 1)
  343. PCR with long and complex primers - (reply: 2)
  344. Phospholylation of primers - (reply: 1)
  345. LINE1 OFR2 Primer Design - (reply: 1)
  346. phosphorylation of primers using PNK - (reply: 1)
  347. Primers for yeast (18S) - (reply: 1)
  348. What's the longest overhanging primer seq. you have used? - (reply: 2)
  349. Primer Concentration to lower Ct - (reply: 1)
  350. Primer Concentration Help - (reply: 4)
  351. Primer Degradation - What exactly happens? (reply: 1)
  352. Primers in 2 exons - (reply: 2)
  353. Problems with designing a primer - (reply: 2)
  354. Probe or Primer info for bovine AQP-1 - (reply: 6)
  355. Different sized product for same BS primer! - (reply: 2)
  356. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  357. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
  358. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  359. primers with restriction site - (reply: 1)
  360. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
  361. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
  362. primer design by generunner DG - (reply: 1)
  363. BSP primers design, help please - (reply: 2)
  364. primers with fluoresent dye PCR cycle question - (reply: 4)
  365. Urgent! Sequencing a mixture of ssDNA fragments of the same gene - ssDNA different in size amplified from a single primer (reply: 2)
  366. Problems with validating primers and low expression genes - (reply: 3)
  367. intron/exon spanning primers - (reply: 1)
  368. MSP primer troubles - (reply: 3)
  369. Designing Primers for multiple Isoforms - (reply: 1)
  370. real time pcr melt curve and primer efficiency problem - (reply: 3)
  371. how to design primer for my n-terminal sequence - hot to design primer for my protein (reply: 2)
  372. Addition of Restriction sites into PCR primers - (reply: 4)
  373. Primer Design in 3non translated version vs. coding region - (reply: 2)
  374. different PCR primers for real-time and classic PCR - (reply: 3)
  375. primers deconamination??? - (reply: 6)
  376. primer check!!! - (reply: 6)
  377. Annealing Temperature of biotinylated primers - (reply: 2)
  378. primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
  379. Primer clean-up - ExoSAP-It isn't working (reply: 6)
  380. Primer dilution - PCR primer dilution (reply: 2)
  381. Long Primers for PCR - (reply: 2)
  382. Primers going "loopy" - (reply: 2)
  383. Conversion of primer unit - ug/ul to uM (reply: 4)
  384. Primer Trouble Shooting - (reply: 5)
  385. Proven Primers Stopped working & now leave smear! - (reply: 2)
  386. a simple primer problem - (reply: 5)
  387. Primers from 3'UTR - (reply: 2)
  388. Designing primers with ESTs - (reply: 2)
  389. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
  390. addition of BclI restriction site to PCR primer - (reply: 6)
  391. How does methprimer calculate primer Tm - (reply: 2)
  392. Phosphorylated primers - (reply: 5)
  393. QPCR - Primer and Probe question - QPCR (reply: 2)
  394. Easy primer question? - Primers (reply: 1)
  395. primer dimer - proplems (reply: 1)
  396. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
  397. Primer efficiency test - (reply: 1)
  398. primer software - (reply: 2)
  399. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
  400. How to know in which exon a primer match? - (reply: 1)
  401. primer annealing - higher annealing= less wrong bounds (reply: 4)
  402. sequencing with forward/reverse primers - (reply: 7)
  403. Adding A overhangs - primer design implications? - (reply: 1)
  404. primer RE over-hang nucleotides - common sequences? (reply: 5)
  405. Forward and reverse primers got very different Tm - what to do? (reply: 11)
  406. primer design problem - (reply: 1)
  407. Problem with primer efficiency analysis - (reply: 4)
  408. primer design - (reply: 1)
  409. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
  410. designing primers for genes not sequenced yet - (reply: 2)
  411. Primer desing - (reply: 2)
  412. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
  413. primer design - (reply: 9)
  414. primer contamination - primer contaminated with ice (reply: 3)
  415. Primer3 vs Primer BLAST - (reply: 3)
  416. Primer design: free energy - (reply: 2)
  417. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  418. Primer optimization for ChIP - (reply: 2)
  419. sequencing primer: T7 or T7promoter? - (reply: 4)
  420. primer tm - tm calculation for tagged primers (reply: 2)
  421. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
  422. Universal 16s rRNA primers needed - (reply: 3)
  423. PCR primers for histone mod ChIP - How to find the regions (reply: 1)
  424. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
  425. adding koazak sequence to primer - (reply: 1)
  426. Designing primers for cloning - After primer designing, how should I perform the PCR? (reply: 15)
  427. Primer design if sequence is unknown for organism - (reply: 5)
  428. adding C terminal tag to reverse primer - (reply: 1)
  429. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
  430. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
  431. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
  432. what primers should I use for DNA sequencing? please help - (reply: 2)
  433. what primers should I use for DNA sequencing?? - (reply: 3)
  434. Is my primer going to work for qRT-PCR? - (reply: 6)
  435. Primers annealing temperatures - (reply: 8)
  436. Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
  437. Synthesize own Oligo-dT primers - (reply: 6)
  438. Testing primer specificity... - ...without gDNA of target organisms (reply: 6)
  439. primer design - Primer design for PCR (reply: 1)
  440. How are primers designed? - (reply: 4)
  441. need help on primer calcuation soon - (reply: 1)
  442. primer design - (reply: 6)
  443. 23S rRNA Primer - need primer sequence (reply: 1)
  444. Freezing primers+SYBR green - (reply: 2)
  445. Tagged Primers - (reply: 5)
  446. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
  447. design primer for chip - (reply: 5)
  448. How to quantify oligonucleotide primers using nanodrop ND-1000 software - newbie help (reply: 2)
  449. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  450. reconstitution of primers - (reply: 2)
  451. primer dimer - (reply: 2)
  452. PCR with long primers - (reply: 3)
  453. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  454. Help me! How to design nested BSP primers? - (reply: 4)
  455. Using BLAST to check primers - (reply: 17)
  456. PCR primer - (reply: 2)
  457. Primer design - GC clamp - (reply: 8)
  458. primer dimer or what? - (reply: 2)
  459. Primers and Taqman Probes mixture question - (reply: 1)
  460. designing primers - (reply: 1)
  461. Negative Control Primers for ChIP Assay - (reply: 3)
  462. PCR Cloning-large primers - (reply: 4)
  463. forward and reverse primer - (reply: 4)
  464. Check the primers - (reply: 4)
  465. BSP primers - (reply: 2)
  466. Primer dimer issue in real time PCR - (reply: 21)
  467. RT-PCR primer dimers and cDNA degradation - (reply: 2)
  468. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  469. primer binding - (reply: 1)
  470. weird ChIP primers! - (reply: 1)
  471. PCR primers - (reply: 1)
  472. diluting to final primer concentration - please help correct (reply: 1)
  473. Smear problem with my new primer set - (reply: 2)
  474. Amplifying a gene using a degenerate primer - (reply: 4)
  475. Making Primer Dimers on Purpose - odd I know...but humor me (reply: 5)
  476. Primer Design - Design Primer with DNA Sequence (reply: 1)
  477. PCR arrays=Primers in a plate? - Can any PCR mix be used? (reply: 1)
  478. Primer tm - (reply: 1)
  479. primer design - really necessary? (reply: 2)
  480. Degenerative primers = multiple products? - (reply: 5)
  481. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
  482. Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
  483. primer dimers - (reply: 5)
  484. qPCR primer design - (reply: 5)
  485. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
  486. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
  487. mouse Gapdh chip-qPCR primers for control! - (reply: 7)
  488. Primer stock confusion - (reply: 6)
  489. primer design - primer design question (reply: 2)
  490. Determine annealing temperature of primers - (reply: 14)
  491. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
  492. Validating real time pcr primers - (reply: 2)
  493. BSP,MSP primer design? - (reply: 1)
  494. low gc primers - help with pcr using low gc primers (reply: 6)
  495. Primers and annealing temperature - (reply: 2)
  496. WHich primer to use for sequencing - (reply: 5)
  497. primer design for pBAD topo expression kit - (reply: 2)
  498. primers fpr sequencing - (reply: 4)
  499. PCR with pfu and degenerate primers (?) - (reply: 7)
  500. Draw PCR primer locations - PCR primer (reply: 4)
  501. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
  502. Program for mapping primers to gene - diagram maker, visualize - (reply: 4)
  503. long PCR primer - (reply: 7)
  504. Taqman rtPCR primer and probe design - (reply: 3)
  505. Amplifying plasmid - non specific binding of primer - (reply: 3)
  506. primer design - (reply: 3)
  507. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
  508. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  509. design primer - when I design a primer is it necessary to include the restriction (reply: 1)
  510. Difference in Primer Melting Temperatures - Maximum allowable Tm difference? (reply: 4)
  511. Primers Not working - Real time PCR using SYBR Green (reply: 3)
  512. PCR conditions with three primers - (reply: 1)
  513. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  514. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
  515. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
  516. using the meth primer - (reply: 2)
  517. Generating primers for MSP - (reply: 1)
  518. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  519. Primer design and need help - (reply: 11)
  520. Programme for degenerate primer design - Programme for primer design (reply: 9)
  521. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  522. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)