Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Primer Related Discussions
  1. Primer concentration - (reply: 1)
  2. Efficiency curve of primers - (reply: 1)
  3. Designing Primer for PCR - (reply: 1)
  4. Primer design (Real time PCR) - (reply: 2)
  5. Specific primer problem - (reply: 2)
  6. Faint band and primer dimer in PCR - (reply: 1)
  7. How to check if my primers were degraded? - (reply: 2)
  8. Design of <=40bp primers adding restriction sites and also Gibson Assembly o - (reply: 1)
  9. Primer design HELP! - (reply: 4)
  10. Pre-designed qRT-PCR primers - (reply: 1)
  11. Same primers and different melting peaks for two samples (~1°C appart) - (reply: 2)
  12. Designing primers with his-tag for pET28a+ vector - (reply: 3)
  13. Can someone help me on my primer design? (contains flag tag) - (reply: 2)
  14. testing primers efficiency for rt-PCR - (reply: 1)
  15. Primer sequences with M and Y - how to order? - (reply: 2)
  16. need help calculating stock primer concentration - (reply: 1)
  17. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
  18. methylation specifc primers thermal cycle conditions - (reply: 4)
  19. PCR Amplification Issues and Primer dimer - (reply: 4)
  20. Primer calculation for qPCR - final reaction - (reply: 1)
  21. Sec. structure in primers - (reply: 1)
  22. PCR master mix containing primers and Taq - (reply: 4)
  23. Primer working with some cDNA samples and not other - (reply: 2)
  24. Primer dilution - (reply: 9)
  25. why not working msp primers - (reply: 4)
  26. Volume of Primers - (reply: 4)
  27. Primer Designing - (reply: 9)
  28. pcr primer design - (reply: 6)
  29. Please help (primer/ probe final volume calculations) - (reply: 1)
  30. Primer design with EcoRI ends - (reply: 2)
  31. PCR with overlapping primers only (primer extention) - (reply: 3)
  32. MSP Primer design - (reply: 5)
  33. How can I check the quality of bacterial primers and their specificity on blast? - (reply: 1)
  34. Primer design - (reply: 1)
  35. Primer non specific binding sites - (reply: 7)
  36. Degenerate primers - (reply: 8)
  37. Primers with 2 different annealing temp - (reply: 1)
  38. Y-linker transgene integration site PCR primer verification - (reply: 1)
  39. NEB TM CALCULATOR PRIMER CONCENTRATION - (reply: 2)
  40. primer design for a gene - (reply: 3)
  41. confirmation for primers dilution - (reply: 10)
  42. Possible primer dimer problem..Need solution!!! - (reply: 2)
  43. confusing with where to put primers and which ordination - (reply: 4)
  44. Help with primer concentration for sequencing - (reply: 1)
  45. Design the primers - (reply: 10)
  46. real time PCR primer design - (reply: 1)
  47. primers for cloning - (reply: 1)
  48. Degenerated Primers and their problems - (reply: 1)
  49. primer design with restriction enzyme - (reply: 1)
  50. Interpreting primer BLAST scores for self-complementary - (reply: 5)
  51. primer dilution help - (reply: 1)
  52. Testing primers on 'unknown' tissue - (reply: 1)
  53. could you help me with my stem loop RT primer? - (reply: 1)
  54. excess amount of primers - (reply: 2)
  55. Dehydrating and Reconstituting primers - (reply: 15)
  56. KIR gene promoters for MSP primers design - (reply: 2)
  57. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
  58. Primer as limiting reagent in PCR reaction - (reply: 2)
  59. Primer Design for RNA probes - (reply: 2)
  60. Nested BSP Primer Design - (reply: 6)
  61. website for primer design - (reply: 1)
  62. How to do a primer dilution - (reply: 10)
  63. Primer design and alternative transcripts - (reply: 2)
  64. NCBI Primer Design - Stringency Issues - (reply: 3)
  65. Different primer concentration in qPCR - (reply: 1)
  66. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
  67. Why when disigning primers for RTPCR, you need to chose a region where introns a - (reply: 1)
  68. Primer Specificity: Testing only one primer - (reply: 4)
  69. tool for comparing many primers pairs - (reply: 4)
  70. Pcr primers - (reply: 7)
  71. Primers have worked well but now getting primer dimers? - (reply: 2)
  72. I cannot design primers on exon-exon junction - (reply: 2)
  73. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  74. Designing primers in UTRs - (reply: 1)
  75. primer design@ buy? - (reply: 2)
  76. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
  77. designing primers( selecting target sequence/amplicon design) - (reply: 3)
  78. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  79. How to design primer to amplify genomic DNA? - (reply: 3)
  80. Primers - (reply: 1)
  81. Primers mix - (reply: 2)
  82. Having problem with primers for qPCR - (reply: 4)
  83. Troubleshooting methylation primers for Bio-Rad PCR - (reply: 3)
  84. No bands despite different primers and conditions and TAqs - (reply: 8)
  85. Equimolar Mix Primer - (reply: 8)
  86. Primer design - (reply: 5)
  87. storage for lyophilized primers - (reply: 1)
  88. Repeated mutagensis primer in site-directed mutagenesis - (reply: 8)
  89. PCR Primer trouble - (reply: 2)
  90. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
  91. finding the corrosponding primers - (reply: 2)
  92. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
  93. Designing primers for a nuclear region to amplify in a species that has no nucle - (reply: 1)
  94. T7 and M13 primers two band amplification - (reply: 3)
  95. fluorescent primer vs fluorescent terminator in sequencing - (reply: 4)
  96. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
  97. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
  98. Melting curve is irregular for primer optimization - (reply: 5)
  99. Designing primers for ABO blood groups - (reply: 1)
  100. RT-PCR primer design - (reply: 7)
  101. Is this primer okay? - (reply: 4)
  102. Can I use Primer as template? - (reply: 1)
  103. Resuspending primers calculation - (reply: 4)
  104. What do you use to check primer secondary structure? - (reply: 3)
  105. mutagenic primers with very high GC content. - (reply: 3)
  106. site-directed mutagenesis primer Tm - (reply: 4)
  107. Random vs oligo primer in preamplification RT - (reply: 1)
  108. Primer concentration - stupid question - (reply: 3)
  109. Primer Design, help i´m New - (reply: 4)
  110. RT-qPCR primer problem - (reply: 4)
  111. [Video] Using NCBI for RT-PCR Primer Design - (reply: 1)
  112. Designing cloning primers for DNMT - (reply: 2)
  113. Are these primer products good enough for qPCR? - (reply: 3)
  114. Can't get PCR with large overhang primers to work - (reply: 8)
  115. Primer Efficiency across runs - (reply: 1)
  116. Designing PCR Primers for cloning - (reply: 17)
  117. Creating primers to add restriction sites to vector backbone - (reply: 7)
  118. Some primers dose not work - (reply: 5)
  119. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
  120. Query regarding primers for quick change mutagenesis - (reply: 3)
  121. the storage time for primers - (reply: 9)
  122. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  123. random primers or oligodT - (reply: 4)
  124. To design or use published primers? - (reply: 4)
  125. PCR primer usage (Clonning & cDNA) - (reply: 2)
  126. problem in cloning PCR primer design with restriction site - (reply: 4)
  127. Amplification with U primers, but not with M primers - (reply: 1)
  128. His tag introduction in to gene and primer design - (reply: 2)
  129. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
  130. Design primer from incomplete sequence... - (reply: 2)
  131. Design primer from incomplete sequence... - (reply: 2)
  132. Primer designing for Methylation - (reply: 6)
  133. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
  134. Overlapping sequence PCR primers - (reply: 1)
  135. BSP PCR primer design explained - (reply: 11)
  136. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
  137. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
  138. good 16S univ. primer for qPCR? - (reply: 3)
  139. I need help for designing a pair of primers for this sequence. - (reply: 1)
  140. long primers PCR - (reply: 4)
  141. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
  142. Sequencing Primers and Plasmids - (reply: 3)
  143. Primers to amplify Kan operon from pet28 - (reply: 2)
  144. Tagged primers and a second PCR targetting the tags - (reply: 5)
  145. Barcode generator for PCR primers - (reply: 2)
  146. Biotinylated primers - (reply: 4)
  147. Transcription of PCR products without a primer coupled with promotor sequence - (reply: 4)
  148. How to join two sequencing files taken form forward and reverse primer - (reply: 3)
  149. Control primers for bisulfite-converted DNA - (reply: 3)
  150. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
  151. Is 27F and Anti-Gamma Primer the same thing for 16s? - (reply: 9)
  152. Q regarding primer mix for MSP!! - (reply: 4)
  153. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  154. BSP Primers with M13 Tails - (reply: 1)
  155. issues when designing PCR primers using PRIMER3 - (reply: 8)
  156. Primer catalog/databasing - (reply: 1)
  157. Primer Blast - (reply: 6)
  158. Help designing primers for use in In-fushion cloning - (reply: 4)
  159. Primers Freeze-Thaw - (reply: 16)
  160. primer design using 16s rRNA - (reply: 1)
  161. Primer concentration for cDNA synthesis using GSP - (reply: 1)
  162. Primer Design HELP! - (reply: 3)
  163. Bisulfite PCR doesn't work and the primer seems abnormal - (reply: 6)
  164. How are Primers made? - (reply: 9)
  165. designing methylation specific primers - (reply: 3)
  166. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  167. Primer Annealing Temperatures - (reply: 24)
  168. same primers, different product in conventional PCR and qPCR - (reply: 6)
  169. Different primer optimalization for nested vs direct MSP? - (reply: 3)
  170. Primers amplify genomic DNA but not cDNA - (reply: 2)
  171. Primer BLAST - (reply: 1)
  172. PCR primer design - published primers trustable? - (reply: 8)
  173. question about universal and specific primers for miRNA real time pcr - (reply: 1)
  174. Can we use degenerate bases in BSP primers? - (reply: 1)
  175. How to check primer sequences using BLAST and other tools? - (reply: 2)
  176. Primer Check? - (reply: 2)
  177. Validation of PCR primers/ probes - (reply: 6)
  178. Two reverse primer sequences for a single forward primer - (reply: 1)
  179. PCR with more than one primer - (reply: 1)
  180. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  181. RT-PCR primer design - (reply: 1)
  182. No or very weak band using 16s primers - (reply: 1)
  183. No PCR amplification with b-actin primers - (reply: 1)
  184. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
  185. Primer Design Help: GFP primers for Arabidopsis - (reply: 1)
  186. No PCR amplified with long primers - (reply: 10)
  187. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  188. another primer dilution question - (reply: 2)
  189. Primer Efficiency - (reply: 2)
  190. oligo(dt) 15 vs random primers - (reply: 3)
  191. primer dilutions - (reply: 4)
  192. Genotyping PCR primers - (reply: 1)
  193. MSP Primers not working - (reply: 6)
  194. Primers too dilute - (reply: 1)
  195. Cloning HELP- Possible Primer issues - (reply: 6)
  196. PCR primer pairs - university exam - (reply: 7)
  197. Help with primer design through PRIMER3 - (reply: 4)
  198. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  199. Tm of primers - prediction and verification - (reply: 1)
  200. how to design primers for 16sr RNA - (reply: 24)
  201. Help with primer design - (reply: 3)
  202. Primer Tm calculation. - (reply: 1)
  203. Running Primers on Agarose Gel - (reply: 6)
  204. PCR product now not getting with same primers and other PCR conditions - (reply: 4)
  205. universal primers for PCR ribotyping - (reply: 6)
  206. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  207. Primer design: Tm above or below Ta? - (reply: 5)
  208. Oligo primers for shRNA construction - (reply: 1)
  209. Design primers for expression cloning - (reply: 11)
  210. primer dilution query - (reply: 2)
  211. Using BLAST to check primers - (reply: 1)
  212. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  213. What is the function of a third primer in a PCR mix?? - (reply: 2)
  214. How can we design a primer ? step by step method - (reply: 4)
  215. EPIGENETICS-NEWBIE-Weird/too close primers for pyrosequencing assay - (reply: 2)
  216. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
  217. Is it possible to amplify cDNA with hexamer primers or oligo dT primers to have - (reply: 1)
  218. annealing temperature for 3 primer pairs? - (reply: 4)
  219. Impact of primer sequence on TA cloning - (reply: 3)
  220. Pcr primers - (reply: 2)
  221. RT-Q PCR primer design question - (reply: 2)
  222. multiplex rtqPCR and custom primer/probe design - (reply: 2)
  223. big differnece in my primers Tm - (reply: 2)
  224. Designing primers for the ORFs - (reply: 9)
  225. PCR with phophorylated primers - (reply: 1)
  226. Problem with New qPCR primers - help! - (reply: 6)
  227. Designing primers for miRNA located on minus strand. - (reply: 2)
  228. Plasmid sequencing without primers - (reply: 1)
  229. RT-PCR primers and the poly(A) tail - (reply: 2)
  230. overlapping primer - (reply: 1)
  231. PCR primers help - (reply: 5)
  232. Primer dimers - (reply: 2)
  233. Primer validation and PCR efficiency - (reply: 4)
  234. should I design primers spanning both intron and exon - (reply: 2)
  235. PCR with a very long and a short primer - (reply: 5)
  236. Comparison between two primers pairs? Is it possible? - (reply: 3)
  237. Rt-PCR primer design - (reply: 7)
  238. Length of non-binding sequence in primers with RE site - (reply: 6)
  239. Orientation of primers - (reply: 2)
  240. methylation analysis with MS-HRM (primer design) - (reply: 1)
  241. primer resuspension - (reply: 2)
  242. Help designing primers with restriction sites - (reply: 3)
  243. Why primers shouldn't have Tm difference greater than 5°C? - (reply: 5)
  244. PCR primer with no extra DNA end - (reply: 5)
  245. Tm calculation for primers with RE sites and overhangs - (reply: 4)
  246. Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
  247. checking msp primers - (reply: 2)
  248. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  249. Another primer dimers problem - (reply: 23)
  250. MSP U primers very difficult to amplify... - (reply: 4)
  251. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
  252. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  253. Primer design - (reply: 3)
  254. single primer PCR - (reply: 5)
  255. Primer concentration - (reply: 4)
  256. Primer 3' mismatch - Strange experience (reply: 9)
  257. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
  258. PRIMER DILUTION - STOCK SOLUTION TO A WORKING STANDARD (reply: 2)
  259. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
  260. Are my MSP primers good? - MSP primers design by Methyl Primer Express v1.0 (reply: 2)
  261. Primer dilution --> problem.. - (reply: 16)
  262. Sybr green RT-qPCR primer - (reply: 1)
  263. Sybr green RT-qPCR primer - (reply: 1)
  264. primer design - microalgae (reply: 2)
  265. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
  266. how to overcome primer contamination - need protocol (reply: 11)
  267. RT-PCR primer design - Primer design and evaluation (reply: 7)
  268. STANDARDISATION OF MULTIPLEX PCR - IN order to standardise a multiplex PCR for 3 set of primers (reply: 3)
  269. Primer,probes dilution - (reply: 3)
  270. PCR: Cloning Primers - (reply: 8)
  271. Exon Spanning PCR Primers - (reply: 1)
  272. Primer sequences - (reply: 1)
  273. PCR Primers - (reply: 2)
  274. How to blast methylation specific and unspecific primers - (reply: 1)
  275. How to check primers are of correct sequence or not? - (reply: 2)
  276. Primers... - (reply: 1)
  277. When are primer dimers a problem? - (reply: 1)
  278. primer with higher melting temperature - (reply: 2)
  279. Problems designing primers for BSP - (reply: 2)
  280. qPCR primer design - possible off-target (reply: 1)
  281. Primer dimer formation and real-time PCR - (reply: 7)
  282. Primer Design Urgent! - (reply: 1)
  283. how to know my primer's sense ??? - help ! (reply: 2)
  284. intron spanning primers for non model organisms - (reply: 2)
  285. Primer design - (reply: 2)
  286. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
  287. Primer seq - (reply: 2)
  288. oligo dt and random primer - (reply: 3)
  289. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
  290. Primers and self and hetero dimers - (reply: 1)
  291. Primers for qPCR - (reply: 3)
  292. RT-PCR primer - (reply: 3)
  293. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
  294. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  295. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  296. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  297. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  298. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  299. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
  300. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
  301. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
  302. ChIP primer design - (reply: 1)
  303. Bisulfite sequencing primer design query (probably simple) - (reply: 4)
  304. Question about Primers - (reply: 3)
  305. Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
  306. designing MSP primers (dimers NOOOOOOOO!) - (reply: 1)
  307. Primer design with a tag - (reply: 4)
  308. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
  309. Combining forward and reverse primer gives different size on gel - (reply: 2)
  310. primers for fungal identification - (reply: 9)
  311. difference between PCR primers and sequencing primers - (reply: 1)
  312. Sense and antisense DNA, and primer design - (reply: 6)
  313. Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
  314. Question about RealTime PCR Primer Design - (reply: 1)
  315. Designing a Primer with a RS that has a W - (reply: 1)
  316. Primers stopped working!? - PCR Primers (reply: 6)
  317. NFK beta primers - (reply: 4)
  318. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
  319. Primer design - Primer design (reply: 2)
  320. Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
  321. Real Time PCR Primers and Probes - (reply: 2)
  322. Need help - How we can design primer for miRNA (reply: 1)
  323. primer and promoter sequences - (reply: 1)
  324. designing of primers - (reply: 2)
  325. Primer Tm differences/ PCR - (reply: 1)
  326. Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
  327. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
  328. d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
  329. Designing primer to remove his-tag - (reply: 1)
  330. PCR Primer Dilution from F+R - (reply: 1)
  331. primer reconstitution - primer reconstitution (reply: 4)
  332. Primer design for insert amplification - Is this correct? (reply: 2)
  333. HRM primers - (reply: 1)
  334. primer design - (reply: 2)
  335. New to sequencing, primer design - (reply: 2)
  336. Quickchange mutagenesis primer - (reply: 3)
  337. pcr / primer theory in bisulfite sequencing - (reply: 4)
  338. Primer Design..need very basic advice. - Please help, I cant figure this out for the life of me. (reply: 2)
  339. Problems with the Specificity of the Primers - (reply: 2)
  340. confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
  341. Degenerate primers PCR problem, Please help! - (reply: 2)
  342. Primer design help needed - (reply: 1)
  343. Primers no longer work - (reply: 1)
  344. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
  345. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
  346. Calculating Primer concentrations for PCR - Is there an easy way to do this...help (reply: 3)
  347. primer Tm is too high, how tu get pcr product - help! (reply: 21)
  348. Find universal primers in vector - any online tool? (reply: 6)
  349. Picking primers to confirm Illumina meth27 results - (reply: 2)
  350. Colony Pcr - primers - (reply: 5)
  351. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
  352. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  353. Primer Dilution Problem - D'oh! (reply: 2)
  354. Is there any software that can be sued to design degenerate primers - (reply: 1)
  355. Primer Decontamination - (reply: 2)
  356. tools for checking non-specific binding of primers -
    (reply: 1)
  357. Primer designed for ARMS PCR - (reply: 2)
  358. methylation specific PCR - Primers - (reply: 1)
  359. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
  360. Primer Design - aligning sequences for probe/primer design (reply: 2)
  361. PCR with one primer - (reply: 1)
  362. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
  363. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
  364. High Tm on primers - cannot get a product - (reply: 8)
  365. NCBI primer blast problem - (reply: 1)
  366. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
  367. primer sequence problem - (reply: 2)
  368. Finding bisulfite PCR primer sequence - (reply: 3)
  369. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
  370. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
  371. How to design primers to check my candidates in a ChiP assay - (reply: 4)
  372. Primer Reconstitution--does temperature matter? - (reply: 5)
  373. Primer Design MSP, BSP, MS-HRM - (reply: 3)
  374. Primers that do not align at regions with SNPs. Where to find SNPs? - (reply: 2)
  375. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  376. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
  377. Primer design for qPCR - (reply: 2)
  378. Primer design for qPCR - (reply: 1)
  379. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
  380. primer design tips - (reply: 3)
  381. design pcr primer - (reply: 1)
  382. PCR with long and complex primers - (reply: 2)
  383. Phospholylation of primers - (reply: 1)
  384. LINE1 OFR2 Primer Design - (reply: 1)
  385. phosphorylation of primers using PNK - (reply: 1)
  386. Primers for yeast (18S) - (reply: 1)
  387. What's the longest overhanging primer seq. you have used? - (reply: 2)
  388. Primer Concentration to lower Ct - (reply: 1)
  389. Primer Concentration Help - (reply: 4)
  390. Primer Degradation - What exactly happens? (reply: 1)
  391. Primers in 2 exons - (reply: 2)
  392. Problems with designing a primer - (reply: 2)
  393. Probe or Primer info for bovine AQP-1 - (reply: 6)
  394. Different sized product for same BS primer! - (reply: 2)
  395. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  396. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
  397. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  398. primers with restriction site - (reply: 1)
  399. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
  400. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
  401. primer design by generunner DG - (reply: 1)
  402. BSP primers design, help please - (reply: 2)
  403. primers with fluoresent dye PCR cycle question - (reply: 4)
  404. Urgent! Sequencing a mixture of ssDNA fragments of the same gene - ssDNA different in size amplified from a single primer (reply: 2)
  405. Problems with validating primers and low expression genes - (reply: 3)
  406. intron/exon spanning primers - (reply: 1)
  407. MSP primer troubles - (reply: 3)
  408. Designing Primers for multiple Isoforms - (reply: 1)
  409. real time pcr melt curve and primer efficiency problem - (reply: 3)
  410. how to design primer for my n-terminal sequence - hot to design primer for my protein (reply: 2)
  411. Addition of Restriction sites into PCR primers - (reply: 4)
  412. Primer Design in 3´non translated version vs. coding region - (reply: 2)
  413. different PCR primers for real-time and classic PCR - (reply: 3)
  414. primers deconamination??? - (reply: 6)
  415. primer check!!! - (reply: 6)
  416. Annealing Temperature of biotinylated primers - (reply: 2)
  417. primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
  418. Primer clean-up - ExoSAP-It isn't working (reply: 6)
  419. Primer dilution - PCR primer dilution (reply: 2)
  420. Long Primers for PCR - (reply: 2)
  421. Primers going "loopy" - (reply: 2)
  422. Conversion of primer unit - ug/ul to uM (reply: 4)
  423. Primer Trouble Shooting - (reply: 5)
  424. Proven Primers Stopped working & now leave smear! - (reply: 2)
  425. a simple primer problem - (reply: 5)
  426. Primers from 3'UTR - (reply: 2)
  427. Designing primers with ESTs - (reply: 2)
  428. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
  429. addition of BclI restriction site to PCR primer - (reply: 6)
  430. How does methprimer calculate primer Tm - (reply: 2)
  431. Phosphorylated primers - (reply: 5)
  432. QPCR - Primer and Probe question - QPCR (reply: 2)
  433. Easy primer question? - Primers (reply: 1)
  434. primer dimer - proplems (reply: 1)
  435. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
  436. Primer efficiency test - (reply: 1)
  437. primer software - (reply: 2)
  438. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
  439. How to know in which exon a primer match? - (reply: 1)
  440. primer annealing - higher annealing= less wrong bounds (reply: 4)
  441. sequencing with forward/reverse primers - (reply: 7)
  442. Adding A overhangs - primer design implications? - (reply: 1)
  443. primer RE over-hang nucleotides - common sequences? (reply: 5)
  444. Forward and reverse primers got very different Tm - what to do? (reply: 11)
  445. primer design problem - (reply: 1)
  446. Problem with primer efficiency analysis - (reply: 4)
  447. primer design - (reply: 1)
  448. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
  449. designing primers for genes not sequenced yet - (reply: 2)
  450. Primer desing - (reply: 2)
  451. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
  452. primer design - (reply: 9)
  453. primer contamination - primer contaminated with ice (reply: 3)
  454. Primer3 vs Primer BLAST - (reply: 3)
  455. Primer design: free energy - (reply: 2)
  456. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  457. Primer optimization for ChIP - (reply: 2)
  458. sequencing primer: T7 or T7promoter? - (reply: 4)
  459. primer tm - tm calculation for tagged primers (reply: 2)
  460. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
  461. Universal 16s rRNA primers needed - (reply: 3)
  462. PCR primers for histone mod ChIP - How to find the regions (reply: 1)
  463. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
  464. adding koazak sequence to primer - (reply: 1)
  465. Designing primers for cloning - After primer designing, how should I perform the PCR? (reply: 15)
  466. Primer design if sequence is unknown for organism - (reply: 5)
  467. adding C terminal tag to reverse primer - (reply: 1)
  468. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
  469. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
  470. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
  471. what primers should I use for DNA sequencing? please help - (reply: 2)
  472. what primers should I use for DNA sequencing?? - (reply: 3)
  473. Is my primer going to work for qRT-PCR? - (reply: 6)
  474. Primers annealing temperatures - (reply: 8)
  475. Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
  476. Synthesize own Oligo-dT primers - (reply: 6)
  477. Testing primer specificity... - ...without gDNA of target organisms (reply: 6)
  478. primer design - Primer design for PCR (reply: 1)
  479. How are primers designed? - (reply: 4)
  480. need help on primer calcuation soon - (reply: 1)
  481. primer design - (reply: 6)
  482. 23S rRNA Primer - need primer sequence (reply: 1)
  483. Freezing primers+SYBR green - (reply: 2)
  484. Tagged Primers - (reply: 5)
  485. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
  486. design primer for chip - (reply: 5)
  487. How to quantify oligonucleotide primers using nanodrop ND-1000 software - newbie help (reply: 2)
  488. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  489. reconstitution of primers - (reply: 2)
  490. primer dimer - (reply: 2)
  491. PCR with long primers - (reply: 3)
  492. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  493. Help me! How to design nested BSP primers? - (reply: 4)
  494. Using BLAST to check primers - (reply: 17)
  495. PCR primer - (reply: 2)
  496. Primer design - GC clamp - (reply: 8)
  497. primer dimer or what? - (reply: 2)
  498. Primers and Taqman Probes mixture question - (reply: 1)
  499. designing primers - (reply: 1)
  500. Negative Control Primers for ChIP Assay - (reply: 3)
  501. PCR Cloning-large primers - (reply: 4)
  502. forward and reverse primer - (reply: 4)
  503. Check the primers - (reply: 4)
  504. BSP primers - (reply: 2)
  505. Primer dimer issue in real time PCR - (reply: 21)
  506. RT-PCR primer dimers and cDNA degradation - (reply: 2)
  507. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  508. primer binding - (reply: 1)
  509. weird ChIP primers! - (reply: 1)
  510. PCR primers - (reply: 1)
  511. diluting to final primer concentration - please help correct (reply: 1)
  512. Smear problem with my new primer set - (reply: 2)
  513. Amplifying a gene using a degenerate primer - (reply: 4)
  514. Making Primer Dimers on Purpose - odd I know...but humor me (reply: 5)
  515. Primer Design - Design Primer with DNA Sequence (reply: 1)
  516. PCR arrays=Primers in a plate? - Can any PCR mix be used? (reply: 1)
  517. Primer tm - (reply: 1)
  518. primer design - really necessary? (reply: 2)
  519. Degenerative primers = multiple products? - (reply: 5)
  520. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
  521. Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
  522. primer dimers - (reply: 5)
  523. qPCR primer design - (reply: 5)
  524. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
  525. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
  526. mouse Gapdh chip-qPCR primers for control! - (reply: 7)
  527. Primer stock confusion - (reply: 6)
  528. primer design - primer design question (reply: 2)
  529. Determine annealing temperature of primers - (reply: 14)
  530. real time PCR primer designing problems,again - is NCBI's primer blast believable enough？ (reply: 3)
  531. Validating real time pcr primers - (reply: 2)
  532. BSP,MSP primer design? - (reply: 1)
  533. low gc primers - help with pcr using low gc primers (reply: 6)
  534. Primers and annealing temperature - (reply: 2)
  535. WHich primer to use for sequencing - (reply: 5)
  536. primer design for pBAD topo expression kit - (reply: 2)
  537. primers fpr sequencing - (reply: 4)
  538. PCR with pfu and degenerate primers (?) - (reply: 7)
  539. Draw PCR primer locations - PCR primer (reply: 4)
  540. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
  541. Program for mapping primers to gene - diagram maker, visualize - (reply: 4)
  542. long PCR primer - (reply: 7)
  543. Taqman rtPCR primer and probe design - (reply: 3)
  544. Amplifying plasmid - non specific binding of primer - (reply: 3)
  545. primer design - (reply: 3)
  546. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
  547. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  548. design primer - when I design a primer is it necessary to include the restriction (reply: 1)
  549. Difference in Primer Melting Temperatures - Maximum allowable Tm difference? (reply: 4)
  550. Primers Not working - Real time PCR using SYBR Green (reply: 3)
  551. PCR conditions with three primers - (reply: 1)
  552. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  553. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
  554. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
  555. using the meth primer - (reply: 2)
  556. Generating primers for MSP - (reply: 1)
  557. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  558. Primer design and need help - (reply: 11)
  559. Programme for degenerate primer design - Programme for primer design (reply: 9)
  560. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  561. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)