Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Primer Related Discussions
  1. Primer dilution - (reply: 7)
  2. why not working msp primers - (reply: 3)
  3. Volume of Primers - (reply: 4)
  4. Primer Designing - (reply: 9)
  5. pcr primer design - (reply: 6)
  6. Please help (primer/ probe final volume calculations) - (reply: 1)
  7. Primer design with EcoRI ends - (reply: 2)
  8. PCR with overlapping primers only (primer extention) - (reply: 3)
  9. MSP Primer design - (reply: 4)
  10. How can I check the quality of bacterial primers and their specificity on blast? - (reply: 1)
  11. Primer design - (reply: 1)
  12. Primer non specific binding sites - (reply: 7)
  13. Degenerate primers - (reply: 8)
  14. Primers with 2 different annealing temp - (reply: 1)
  15. Y-linker transgene integration site PCR primer verification - (reply: 1)
  16. NEB TM CALCULATOR PRIMER CONCENTRATION - (reply: 2)
  17. primer design for a gene - (reply: 3)
  18. confirmation for primers dilution - (reply: 10)
  19. Possible primer dimer problem..Need solution!!! - (reply: 2)
  20. confusing with where to put primers and which ordination - (reply: 4)
  21. Help with primer concentration for sequencing - (reply: 1)
  22. Design the primers - (reply: 10)
  23. real time PCR primer design - (reply: 1)
  24. primers for cloning - (reply: 1)
  25. Degenerated Primers and their problems - (reply: 1)
  26. primer design with restriction enzyme - (reply: 1)
  27. Interpreting primer BLAST scores for self-complementary - (reply: 5)
  28. primer dilution help - (reply: 1)
  29. Testing primers on 'unknown' tissue - (reply: 1)
  30. could you help me with my stem loop RT primer? - (reply: 1)
  31. excess amount of primers - (reply: 2)
  32. Dehydrating and Reconstituting primers - (reply: 15)
  33. KIR gene promoters for MSP primers design - (reply: 2)
  34. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
  35. Primer as limiting reagent in PCR reaction - (reply: 2)
  36. Primer Design for RNA probes - (reply: 2)
  37. Nested BSP Primer Design - (reply: 6)
  38. website for primer design - (reply: 1)
  39. How to do a primer dilution - (reply: 10)
  40. Primer design and alternative transcripts - (reply: 2)
  41. NCBI Primer Design - Stringency Issues - (reply: 3)
  42. Different primer concentration in qPCR - (reply: 1)
  43. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
  44. Why when disigning primers for RTPCR, you need to chose a region where introns a - (reply: 1)
  45. Primer Specificity: Testing only one primer - (reply: 4)
  46. tool for comparing many primers pairs - (reply: 4)
  47. Pcr primers - (reply: 7)
  48. Primers have worked well but now getting primer dimers? - (reply: 2)
  49. I cannot design primers on exon-exon junction - (reply: 2)
  50. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  51. Designing primers in UTRs - (reply: 1)
  52. primer design@ buy? - (reply: 2)
  53. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
  54. designing primers( selecting target sequence/amplicon design) - (reply: 3)
  55. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  56. How to design primer to amplify genomic DNA? - (reply: 3)
  57. Primers - (reply: 1)
  58. Primers mix - (reply: 2)
  59. Having problem with primers for qPCR - (reply: 4)
  60. Troubleshooting methylation primers for Bio-Rad PCR - (reply: 3)
  61. No bands despite different primers and conditions and TAqs - (reply: 8)
  62. Equimolar Mix Primer - (reply: 8)
  63. Primer design - (reply: 5)
  64. storage for lyophilized primers - (reply: 1)
  65. Repeated mutagensis primer in site-directed mutagenesis - (reply: 8)
  66. PCR Primer trouble - (reply: 2)
  67. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
  68. finding the corrosponding primers - (reply: 2)
  69. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
  70. Designing primers for a nuclear region to amplify in a species that has no nucle - (reply: 1)
  71. T7 and M13 primers two band amplification - (reply: 3)
  72. fluorescent primer vs fluorescent terminator in sequencing - (reply: 4)
  73. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
  74. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
  75. Melting curve is irregular for primer optimization - (reply: 5)
  76. Designing primers for ABO blood groups - (reply: 1)
  77. RT-PCR primer design - (reply: 7)
  78. Is this primer okay? - (reply: 4)
  79. Can I use Primer as template? - (reply: 1)
  80. Resuspending primers calculation - (reply: 4)
  81. What do you use to check primer secondary structure? - (reply: 3)
  82. mutagenic primers with very high GC content. - (reply: 3)
  83. site-directed mutagenesis primer Tm - (reply: 4)
  84. Random vs oligo primer in preamplification RT - (reply: 1)
  85. Primer concentration - stupid question - (reply: 3)
  86. Primer Design, help im New - (reply: 4)
  87. RT-qPCR primer problem - (reply: 4)
  88. [Video] Using NCBI for RT-PCR Primer Design - (reply: 1)
  89. Designing cloning primers for DNMT - (reply: 2)
  90. Are these primer products good enough for qPCR? - (reply: 3)
  91. Can't get PCR with large overhang primers to work - (reply: 8)
  92. Primer Efficiency across runs - (reply: 1)
  93. Designing PCR Primers for cloning - (reply: 17)
  94. Creating primers to add restriction sites to vector backbone - (reply: 7)
  95. Some primers dose not work - (reply: 5)
  96. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
  97. Query regarding primers for quick change mutagenesis - (reply: 3)
  98. the storage time for primers - (reply: 9)
  99. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  100. random primers or oligodT - (reply: 4)
  101. To design or use published primers? - (reply: 4)
  102. PCR primer usage (Clonning & cDNA) - (reply: 2)
  103. problem in cloning PCR primer design with restriction site - (reply: 4)
  104. Amplification with U primers, but not with M primers - (reply: 1)
  105. His tag introduction in to gene and primer design - (reply: 2)
  106. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
  107. Design primer from incomplete sequence... - (reply: 2)
  108. Design primer from incomplete sequence... - (reply: 2)
  109. Primer designing for Methylation - (reply: 6)
  110. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
  111. Overlapping sequence PCR primers - (reply: 1)
  112. BSP PCR primer design explained - (reply: 11)
  113. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
  114. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
  115. good 16S univ. primer for qPCR? - (reply: 3)
  116. I need help for designing a pair of primers for this sequence. - (reply: 1)
  117. long primers PCR - (reply: 4)
  118. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
  119. Sequencing Primers and Plasmids - (reply: 3)
  120. Primers to amplify Kan operon from pet28 - (reply: 2)
  121. Tagged primers and a second PCR targetting the tags - (reply: 5)
  122. Barcode generator for PCR primers - (reply: 2)
  123. Biotinylated primers - (reply: 4)
  124. Transcription of PCR products without a primer coupled with promotor sequence - (reply: 4)
  125. How to join two sequencing files taken form forward and reverse primer - (reply: 3)
  126. Control primers for bisulfite-converted DNA - (reply: 3)
  127. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
  128. Is 27F and Anti-Gamma Primer the same thing for 16s? - (reply: 9)
  129. Q regarding primer mix for MSP!! - (reply: 4)
  130. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  131. BSP Primers with M13 Tails - (reply: 1)
  132. issues when designing PCR primers using PRIMER3 - (reply: 8)
  133. Primer catalog/databasing - (reply: 1)
  134. Primer Blast - (reply: 6)
  135. Help designing primers for use in In-fushion cloning - (reply: 4)
  136. Primers Freeze-Thaw - (reply: 16)
  137. primer design using 16s rRNA - (reply: 1)
  138. Primer concentration for cDNA synthesis using GSP - (reply: 1)
  139. Primer Design HELP! - (reply: 3)
  140. Bisulfite PCR doesn't work and the primer seems abnormal - (reply: 6)
  141. How are Primers made? - (reply: 9)
  142. designing methylation specific primers - (reply: 3)
  143. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  144. Primer Annealing Temperatures - (reply: 24)
  145. same primers, different product in conventional PCR and qPCR - (reply: 6)
  146. Different primer optimalization for nested vs direct MSP? - (reply: 3)
  147. Primers amplify genomic DNA but not cDNA - (reply: 2)
  148. Primer BLAST - (reply: 1)
  149. PCR primer design - published primers trustable? - (reply: 8)
  150. question about universal and specific primers for miRNA real time pcr - (reply: 1)
  151. Can we use degenerate bases in BSP primers? - (reply: 1)
  152. How to check primer sequences using BLAST and other tools? - (reply: 2)
  153. Primer Check? - (reply: 2)
  154. Validation of PCR primers/ probes - (reply: 6)
  155. Two reverse primer sequences for a single forward primer - (reply: 1)
  156. PCR with more than one primer - (reply: 1)
  157. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  158. RT-PCR primer design - (reply: 1)
  159. No or very weak band using 16s primers - (reply: 1)
  160. No PCR amplification with b-actin primers - (reply: 1)
  161. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
  162. Primer Design Help: GFP primers for Arabidopsis - (reply: 1)
  163. No PCR amplified with long primers - (reply: 10)
  164. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  165. another primer dilution question - (reply: 2)
  166. Primer Efficiency - (reply: 2)
  167. oligo(dt) 15 vs random primers - (reply: 3)
  168. primer dilutions - (reply: 4)
  169. Genotyping PCR primers - (reply: 1)
  170. MSP Primers not working - (reply: 6)
  171. Primers too dilute - (reply: 1)
  172. Cloning HELP- Possible Primer issues - (reply: 6)
  173. PCR primer pairs - university exam - (reply: 7)
  174. Help with primer design through PRIMER3 - (reply: 4)
  175. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  176. Tm of primers - prediction and verification - (reply: 1)
  177. how to design primers for 16sr RNA - (reply: 24)
  178. Help with primer design - (reply: 3)
  179. Primer Tm calculation. - (reply: 1)
  180. Running Primers on Agarose Gel - (reply: 6)
  181. PCR product now not getting with same primers and other PCR conditions - (reply: 4)
  182. universal primers for PCR ribotyping - (reply: 6)
  183. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  184. Primer design: Tm above or below Ta? - (reply: 5)
  185. Oligo primers for shRNA construction - (reply: 1)
  186. Design primers for expression cloning - (reply: 11)
  187. primer dilution query - (reply: 2)
  188. Using BLAST to check primers - (reply: 1)
  189. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  190. What is the function of a third primer in a PCR mix?? - (reply: 2)
  191. How can we design a primer ? step by step method - (reply: 4)
  192. EPIGENETICS-NEWBIE-Weird/too close primers for pyrosequencing assay - (reply: 2)
  193. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
  194. Is it possible to amplify cDNA with hexamer primers or oligo dT primers to have - (reply: 1)
  195. annealing temperature for 3 primer pairs? - (reply: 4)
  196. Impact of primer sequence on TA cloning - (reply: 3)
  197. Pcr primers - (reply: 2)
  198. RT-Q PCR primer design question - (reply: 2)
  199. multiplex rtqPCR and custom primer/probe design - (reply: 2)
  200. big differnece in my primers Tm - (reply: 2)
  201. Designing primers for the ORFs - (reply: 9)
  202. PCR with phophorylated primers - (reply: 1)
  203. Problem with New qPCR primers - help! - (reply: 6)
  204. Designing primers for miRNA located on minus strand. - (reply: 2)
  205. Plasmid sequencing without primers - (reply: 1)
  206. RT-PCR primers and the poly(A) tail - (reply: 2)
  207. overlapping primer - (reply: 1)
  208. PCR primers help - (reply: 5)
  209. Primer dimers - (reply: 2)
  210. Primer validation and PCR efficiency - (reply: 4)
  211. should I design primers spanning both intron and exon - (reply: 2)
  212. PCR with a very long and a short primer - (reply: 5)
  213. Comparison between two primers pairs? Is it possible? - (reply: 3)
  214. Rt-PCR primer design - (reply: 7)
  215. Length of non-binding sequence in primers with RE site - (reply: 6)
  216. Orientation of primers - (reply: 2)
  217. methylation analysis with MS-HRM (primer design) - (reply: 1)
  218. primer resuspension - (reply: 2)
  219. Help designing primers with restriction sites - (reply: 3)
  220. Why primers shouldn't have Tm difference greater than 5C? - (reply: 5)
  221. PCR primer with no extra DNA end - (reply: 5)
  222. Tm calculation for primers with RE sites and overhangs - (reply: 4)
  223. Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
  224. checking msp primers - (reply: 2)
  225. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  226. Another primer dimers problem - (reply: 23)
  227. MSP U primers very difficult to amplify... - (reply: 4)
  228. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
  229. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  230. Primer design - (reply: 3)
  231. single primer PCR - (reply: 5)
  232. Primer concentration - (reply: 4)
  233. Primer 3' mismatch - Strange experience (reply: 9)
  234. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
  235. PRIMER DILUTION - STOCK SOLUTION TO A WORKING STANDARD (reply: 2)
  236. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
  237. Are my MSP primers good? - MSP primers design by Methyl Primer Express v1.0 (reply: 2)
  238. Primer dilution --> problem.. - (reply: 16)
  239. Sybr green RT-qPCR primer - (reply: 1)
  240. Sybr green RT-qPCR primer - (reply: 1)
  241. primer design - microalgae (reply: 2)
  242. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
  243. how to overcome primer contamination - need protocol (reply: 11)
  244. RT-PCR primer design - Primer design and evaluation (reply: 7)
  245. STANDARDISATION OF MULTIPLEX PCR - IN order to standardise a multiplex PCR for 3 set of primers (reply: 3)
  246. Primer,probes dilution - (reply: 3)
  247. PCR: Cloning Primers - (reply: 8)
  248. Exon Spanning PCR Primers - (reply: 1)
  249. Primer sequences - (reply: 1)
  250. PCR Primers - (reply: 2)
  251. How to blast methylation specific and unspecific primers - (reply: 1)
  252. How to check primers are of correct sequence or not? - (reply: 2)
  253. Primers... - (reply: 1)
  254. When are primer dimers a problem? - (reply: 1)
  255. primer with higher melting temperature - (reply: 2)
  256. Problems designing primers for BSP - (reply: 2)
  257. qPCR primer design - possible off-target (reply: 1)
  258. Primer dimer formation and real-time PCR - (reply: 7)
  259. Primer Design Urgent! - (reply: 1)
  260. how to know my primer's sense ??? - help ! (reply: 2)
  261. intron spanning primers for non model organisms - (reply: 2)
  262. Primer design - (reply: 2)
  263. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
  264. Primer seq - (reply: 2)
  265. oligo dt and random primer - (reply: 3)
  266. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
  267. Primers and self and hetero dimers - (reply: 1)
  268. Primers for qPCR - (reply: 3)
  269. RT-PCR primer - (reply: 3)
  270. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
  271. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  272. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  273. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  274. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  275. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  276. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
  277. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
  278. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
  279. ChIP primer design - (reply: 1)
  280. Bisulfite sequencing primer design query (probably simple) - (reply: 4)
  281. Question about Primers - (reply: 3)
  282. Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
  283. designing MSP primers (dimers NOOOOOOOO!) - (reply: 1)
  284. Primer design with a tag - (reply: 4)
  285. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
  286. Combining forward and reverse primer gives different size on gel - (reply: 2)
  287. primers for fungal identification - (reply: 9)
  288. difference between PCR primers and sequencing primers - (reply: 1)
  289. Sense and antisense DNA, and primer design - (reply: 6)
  290. Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
  291. Question about RealTime PCR Primer Design - (reply: 1)
  292. Designing a Primer with a RS that has a W - (reply: 1)
  293. Primers stopped working!? - PCR Primers (reply: 6)
  294. NFK beta primers - (reply: 4)
  295. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
  296. Primer design - Primer design (reply: 2)
  297. Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
  298. Real Time PCR Primers and Probes - (reply: 2)
  299. Need help - How we can design primer for miRNA (reply: 1)
  300. primer and promoter sequences - (reply: 1)
  301. designing of primers - (reply: 2)
  302. Primer Tm differences/ PCR - (reply: 1)
  303. Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
  304. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
  305. d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
  306. Designing primer to remove his-tag - (reply: 1)
  307. PCR Primer Dilution from F+R - (reply: 1)
  308. primer reconstitution - primer reconstitution (reply: 4)
  309. Primer design for insert amplification - Is this correct? (reply: 2)
  310. HRM primers - (reply: 1)
  311. primer design - (reply: 2)
  312. New to sequencing, primer design - (reply: 2)
  313. Quickchange mutagenesis primer - (reply: 3)
  314. pcr / primer theory in bisulfite sequencing - (reply: 4)
  315. Primer Design..need very basic advice. - Please help, I cant figure this out for the life of me. (reply: 2)
  316. Problems with the Specificity of the Primers - (reply: 2)
  317. confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
  318. Degenerate primers PCR problem, Please help! - (reply: 2)
  319. Primer design help needed - (reply: 1)
  320. Primers no longer work - (reply: 1)
  321. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
  322. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
  323. Calculating Primer concentrations for PCR - Is there an easy way to do this...help (reply: 3)
  324. primer Tm is too high, how tu get pcr product - help! (reply: 21)
  325. Find universal primers in vector - any online tool? (reply: 6)
  326. Picking primers to confirm Illumina meth27 results - (reply: 2)
  327. Colony Pcr - primers - (reply: 5)
  328. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
  329. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  330. Primer Dilution Problem - D'oh! (reply: 2)
  331. Is there any software that can be sued to design degenerate primers - (reply: 1)
  332. Primer Decontamination - (reply: 2)
  333. tools for checking non-specific binding of primers -
    (reply: 1)
  334. Primer designed for ARMS PCR - (reply: 2)
  335. methylation specific PCR - Primers - (reply: 1)
  336. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
  337. Primer Design - aligning sequences for probe/primer design (reply: 2)
  338. PCR with one primer - (reply: 1)
  339. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
  340. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
  341. High Tm on primers - cannot get a product - (reply: 8)
  342. NCBI primer blast problem - (reply: 1)
  343. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
  344. primer sequence problem - (reply: 2)
  345. Finding bisulfite PCR primer sequence - (reply: 3)
  346. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
  347. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
  348. How to design primers to check my candidates in a ChiP assay - (reply: 4)
  349. Primer Reconstitution--does temperature matter? - (reply: 5)
  350. Primer Design MSP, BSP, MS-HRM - (reply: 3)
  351. Primers that do not align at regions with SNPs. Where to find SNPs? - (reply: 2)
  352. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  353. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
  354. Primer design for qPCR - (reply: 2)
  355. Primer design for qPCR - (reply: 1)
  356. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
  357. primer design tips - (reply: 3)
  358. design pcr primer - (reply: 1)
  359. PCR with long and complex primers - (reply: 2)
  360. Phospholylation of primers - (reply: 1)
  361. LINE1 OFR2 Primer Design - (reply: 1)
  362. phosphorylation of primers using PNK - (reply: 1)
  363. Primers for yeast (18S) - (reply: 1)
  364. What's the longest overhanging primer seq. you have used? - (reply: 2)
  365. Primer Concentration to lower Ct - (reply: 1)
  366. Primer Concentration Help - (reply: 4)
  367. Primer Degradation - What exactly happens? (reply: 1)
  368. Primers in 2 exons - (reply: 2)
  369. Problems with designing a primer - (reply: 2)
  370. Probe or Primer info for bovine AQP-1 - (reply: 6)
  371. Different sized product for same BS primer! - (reply: 2)
  372. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  373. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
  374. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  375. primers with restriction site - (reply: 1)
  376. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
  377. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
  378. primer design by generunner DG - (reply: 1)
  379. BSP primers design, help please - (reply: 2)
  380. primers with fluoresent dye PCR cycle question - (reply: 4)
  381. Urgent! Sequencing a mixture of ssDNA fragments of the same gene - ssDNA different in size amplified from a single primer (reply: 2)
  382. Problems with validating primers and low expression genes - (reply: 3)
  383. intron/exon spanning primers - (reply: 1)
  384. MSP primer troubles - (reply: 3)
  385. Designing Primers for multiple Isoforms - (reply: 1)
  386. real time pcr melt curve and primer efficiency problem - (reply: 3)
  387. how to design primer for my n-terminal sequence - hot to design primer for my protein (reply: 2)
  388. Addition of Restriction sites into PCR primers - (reply: 4)
  389. Primer Design in 3non translated version vs. coding region - (reply: 2)
  390. different PCR primers for real-time and classic PCR - (reply: 3)
  391. primers deconamination??? - (reply: 6)
  392. primer check!!! - (reply: 6)
  393. Annealing Temperature of biotinylated primers - (reply: 2)
  394. primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
  395. Primer clean-up - ExoSAP-It isn't working (reply: 6)
  396. Primer dilution - PCR primer dilution (reply: 2)
  397. Long Primers for PCR - (reply: 2)
  398. Primers going "loopy" - (reply: 2)
  399. Conversion of primer unit - ug/ul to uM (reply: 4)
  400. Primer Trouble Shooting - (reply: 5)
  401. Proven Primers Stopped working & now leave smear! - (reply: 2)
  402. a simple primer problem - (reply: 5)
  403. Primers from 3'UTR - (reply: 2)
  404. Designing primers with ESTs - (reply: 2)
  405. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
  406. addition of BclI restriction site to PCR primer - (reply: 6)
  407. How does methprimer calculate primer Tm - (reply: 2)
  408. Phosphorylated primers - (reply: 5)
  409. QPCR - Primer and Probe question - QPCR (reply: 2)
  410. Easy primer question? - Primers (reply: 1)
  411. primer dimer - proplems (reply: 1)
  412. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
  413. Primer efficiency test - (reply: 1)
  414. primer software - (reply: 2)
  415. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
  416. How to know in which exon a primer match? - (reply: 1)
  417. primer annealing - higher annealing= less wrong bounds (reply: 4)
  418. sequencing with forward/reverse primers - (reply: 7)
  419. Adding A overhangs - primer design implications? - (reply: 1)
  420. primer RE over-hang nucleotides - common sequences? (reply: 5)
  421. Forward and reverse primers got very different Tm - what to do? (reply: 11)
  422. primer design problem - (reply: 1)
  423. Problem with primer efficiency analysis - (reply: 4)
  424. primer design - (reply: 1)
  425. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
  426. designing primers for genes not sequenced yet - (reply: 2)
  427. Primer desing - (reply: 2)
  428. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
  429. primer design - (reply: 9)
  430. primer contamination - primer contaminated with ice (reply: 3)
  431. Primer3 vs Primer BLAST - (reply: 3)
  432. Primer design: free energy - (reply: 2)
  433. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  434. Primer optimization for ChIP - (reply: 2)
  435. sequencing primer: T7 or T7promoter? - (reply: 4)
  436. primer tm - tm calculation for tagged primers (reply: 2)
  437. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
  438. Universal 16s rRNA primers needed - (reply: 3)
  439. PCR primers for histone mod ChIP - How to find the regions (reply: 1)
  440. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
  441. adding koazak sequence to primer - (reply: 1)
  442. Designing primers for cloning - After primer designing, how should I perform the PCR? (reply: 15)
  443. Primer design if sequence is unknown for organism - (reply: 5)
  444. adding C terminal tag to reverse primer - (reply: 1)
  445. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
  446. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
  447. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
  448. what primers should I use for DNA sequencing? please help - (reply: 2)
  449. what primers should I use for DNA sequencing?? - (reply: 3)
  450. Is my primer going to work for qRT-PCR? - (reply: 6)
  451. Primers annealing temperatures - (reply: 8)
  452. Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
  453. Synthesize own Oligo-dT primers - (reply: 6)
  454. Testing primer specificity... - ...without gDNA of target organisms (reply: 6)
  455. primer design - Primer design for PCR (reply: 1)
  456. How are primers designed? - (reply: 4)
  457. need help on primer calcuation soon - (reply: 1)
  458. primer design - (reply: 6)
  459. 23S rRNA Primer - need primer sequence (reply: 1)
  460. Freezing primers+SYBR green - (reply: 2)
  461. Tagged Primers - (reply: 5)
  462. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
  463. design primer for chip - (reply: 5)
  464. How to quantify oligonucleotide primers using nanodrop ND-1000 software - newbie help (reply: 2)
  465. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  466. reconstitution of primers - (reply: 2)
  467. primer dimer - (reply: 2)
  468. PCR with long primers - (reply: 3)
  469. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  470. Help me! How to design nested BSP primers? - (reply: 4)
  471. Using BLAST to check primers - (reply: 17)
  472. PCR primer - (reply: 2)
  473. Primer design - GC clamp - (reply: 8)
  474. primer dimer or what? - (reply: 2)
  475. Primers and Taqman Probes mixture question - (reply: 1)
  476. designing primers - (reply: 1)
  477. Negative Control Primers for ChIP Assay - (reply: 3)
  478. PCR Cloning-large primers - (reply: 4)
  479. forward and reverse primer - (reply: 4)
  480. Check the primers - (reply: 4)
  481. BSP primers - (reply: 2)
  482. Primer dimer issue in real time PCR - (reply: 21)
  483. RT-PCR primer dimers and cDNA degradation - (reply: 2)
  484. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  485. primer binding - (reply: 1)
  486. weird ChIP primers! - (reply: 1)
  487. PCR primers - (reply: 1)
  488. diluting to final primer concentration - please help correct (reply: 1)
  489. Smear problem with my new primer set - (reply: 2)
  490. Amplifying a gene using a degenerate primer - (reply: 4)
  491. Making Primer Dimers on Purpose - odd I know...but humor me (reply: 5)
  492. Primer Design - Design Primer with DNA Sequence (reply: 1)
  493. PCR arrays=Primers in a plate? - Can any PCR mix be used? (reply: 1)
  494. Primer tm - (reply: 1)
  495. primer design - really necessary? (reply: 2)
  496. Degenerative primers = multiple products? - (reply: 5)
  497. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
  498. Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
  499. primer dimers - (reply: 5)
  500. qPCR primer design - (reply: 5)
  501. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
  502. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
  503. mouse Gapdh chip-qPCR primers for control! - (reply: 7)
  504. Primer stock confusion - (reply: 6)
  505. primer design - primer design question (reply: 2)
  506. Determine annealing temperature of primers - (reply: 14)
  507. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
  508. Validating real time pcr primers - (reply: 2)
  509. BSP,MSP primer design? - (reply: 1)
  510. low gc primers - help with pcr using low gc primers (reply: 6)
  511. Primers and annealing temperature - (reply: 2)
  512. WHich primer to use for sequencing - (reply: 5)
  513. primer design for pBAD topo expression kit - (reply: 2)
  514. primers fpr sequencing - (reply: 4)
  515. PCR with pfu and degenerate primers (?) - (reply: 7)
  516. Draw PCR primer locations - PCR primer (reply: 4)
  517. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
  518. Program for mapping primers to gene - diagram maker, visualize - (reply: 4)
  519. long PCR primer - (reply: 7)
  520. Taqman rtPCR primer and probe design - (reply: 3)
  521. Amplifying plasmid - non specific binding of primer - (reply: 3)
  522. primer design - (reply: 3)
  523. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
  524. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  525. design primer - when I design a primer is it necessary to include the restriction (reply: 1)
  526. Difference in Primer Melting Temperatures - Maximum allowable Tm difference? (reply: 4)
  527. Primers Not working - Real time PCR using SYBR Green (reply: 3)
  528. PCR conditions with three primers - (reply: 1)
  529. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  530. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
  531. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
  532. using the meth primer - (reply: 2)
  533. Generating primers for MSP - (reply: 1)
  534. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  535. Primer design and need help - (reply: 11)
  536. Programme for degenerate primer design - Programme for primer design (reply: 9)
  537. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  538. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)