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Primer Related Discussions
  1. Faint band and primer dimer in PCR - (reply: 1)
  2. How to check if my primers were degraded? - (reply: 2)
  3. Design of <=40bp primers adding restriction sites and also Gibson Assembly o - (reply: 1)
  4. Primer design HELP! - (reply: 4)
  5. Pre-designed qRT-PCR primers - (reply: 1)
  6. Same primers and different melting peaks for two samples (~1C appart) - (reply: 2)
  7. Designing primers with his-tag for pET28a+ vector - (reply: 3)
  8. Can someone help me on my primer design? (contains flag tag) - (reply: 2)
  9. testing primers efficiency for rt-PCR - (reply: 1)
  10. Primer sequences with M and Y - how to order? - (reply: 2)
  11. need help calculating stock primer concentration - (reply: 1)
  12. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
  13. methylation specifc primers thermal cycle conditions - (reply: 4)
  14. PCR Amplification Issues and Primer dimer - (reply: 4)
  15. Primer calculation for qPCR - final reaction - (reply: 1)
  16. Sec. structure in primers - (reply: 1)
  17. PCR master mix containing primers and Taq - (reply: 4)
  18. Primer working with some cDNA samples and not other - (reply: 2)
  19. Primer dilution - (reply: 7)
  20. why not working msp primers - (reply: 4)
  21. Volume of Primers - (reply: 4)
  22. Primer Designing - (reply: 9)
  23. pcr primer design - (reply: 6)
  24. Please help (primer/ probe final volume calculations) - (reply: 1)
  25. Primer design with EcoRI ends - (reply: 2)
  26. PCR with overlapping primers only (primer extention) - (reply: 3)
  27. MSP Primer design - (reply: 5)
  28. How can I check the quality of bacterial primers and their specificity on blast? - (reply: 1)
  29. Primer design - (reply: 1)
  30. Primer non specific binding sites - (reply: 7)
  31. Degenerate primers - (reply: 8)
  32. Primers with 2 different annealing temp - (reply: 1)
  33. Y-linker transgene integration site PCR primer verification - (reply: 1)
  34. NEB TM CALCULATOR PRIMER CONCENTRATION - (reply: 2)
  35. primer design for a gene - (reply: 3)
  36. confirmation for primers dilution - (reply: 10)
  37. Possible primer dimer problem..Need solution!!! - (reply: 2)
  38. confusing with where to put primers and which ordination - (reply: 4)
  39. Help with primer concentration for sequencing - (reply: 1)
  40. Design the primers - (reply: 10)
  41. real time PCR primer design - (reply: 1)
  42. primers for cloning - (reply: 1)
  43. Degenerated Primers and their problems - (reply: 1)
  44. primer design with restriction enzyme - (reply: 1)
  45. Interpreting primer BLAST scores for self-complementary - (reply: 5)
  46. primer dilution help - (reply: 1)
  47. Testing primers on 'unknown' tissue - (reply: 1)
  48. could you help me with my stem loop RT primer? - (reply: 1)
  49. excess amount of primers - (reply: 2)
  50. Dehydrating and Reconstituting primers - (reply: 15)
  51. KIR gene promoters for MSP primers design - (reply: 2)
  52. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
  53. Primer as limiting reagent in PCR reaction - (reply: 2)
  54. Primer Design for RNA probes - (reply: 2)
  55. Nested BSP Primer Design - (reply: 6)
  56. website for primer design - (reply: 1)
  57. How to do a primer dilution - (reply: 10)
  58. Primer design and alternative transcripts - (reply: 2)
  59. NCBI Primer Design - Stringency Issues - (reply: 3)
  60. Different primer concentration in qPCR - (reply: 1)
  61. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
  62. Why when disigning primers for RTPCR, you need to chose a region where introns a - (reply: 1)
  63. Primer Specificity: Testing only one primer - (reply: 4)
  64. tool for comparing many primers pairs - (reply: 4)
  65. Pcr primers - (reply: 7)
  66. Primers have worked well but now getting primer dimers? - (reply: 2)
  67. I cannot design primers on exon-exon junction - (reply: 2)
  68. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  69. Designing primers in UTRs - (reply: 1)
  70. primer design@ buy? - (reply: 2)
  71. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
  72. designing primers( selecting target sequence/amplicon design) - (reply: 3)
  73. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  74. How to design primer to amplify genomic DNA? - (reply: 3)
  75. Primers - (reply: 1)
  76. Primers mix - (reply: 2)
  77. Having problem with primers for qPCR - (reply: 4)
  78. Troubleshooting methylation primers for Bio-Rad PCR - (reply: 3)
  79. No bands despite different primers and conditions and TAqs - (reply: 8)
  80. Equimolar Mix Primer - (reply: 8)
  81. Primer design - (reply: 5)
  82. storage for lyophilized primers - (reply: 1)
  83. Repeated mutagensis primer in site-directed mutagenesis - (reply: 8)
  84. PCR Primer trouble - (reply: 2)
  85. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
  86. finding the corrosponding primers - (reply: 2)
  87. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
  88. Designing primers for a nuclear region to amplify in a species that has no nucle - (reply: 1)
  89. T7 and M13 primers two band amplification - (reply: 3)
  90. fluorescent primer vs fluorescent terminator in sequencing - (reply: 4)
  91. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
  92. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
  93. Melting curve is irregular for primer optimization - (reply: 5)
  94. Designing primers for ABO blood groups - (reply: 1)
  95. RT-PCR primer design - (reply: 7)
  96. Is this primer okay? - (reply: 4)
  97. Can I use Primer as template? - (reply: 1)
  98. Resuspending primers calculation - (reply: 4)
  99. What do you use to check primer secondary structure? - (reply: 3)
  100. mutagenic primers with very high GC content. - (reply: 3)
  101. site-directed mutagenesis primer Tm - (reply: 4)
  102. Random vs oligo primer in preamplification RT - (reply: 1)
  103. Primer concentration - stupid question - (reply: 3)
  104. Primer Design, help im New - (reply: 4)
  105. RT-qPCR primer problem - (reply: 4)
  106. [Video] Using NCBI for RT-PCR Primer Design - (reply: 1)
  107. Designing cloning primers for DNMT - (reply: 2)
  108. Are these primer products good enough for qPCR? - (reply: 3)
  109. Can't get PCR with large overhang primers to work - (reply: 8)
  110. Primer Efficiency across runs - (reply: 1)
  111. Designing PCR Primers for cloning - (reply: 17)
  112. Creating primers to add restriction sites to vector backbone - (reply: 7)
  113. Some primers dose not work - (reply: 5)
  114. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
  115. Query regarding primers for quick change mutagenesis - (reply: 3)
  116. the storage time for primers - (reply: 9)
  117. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  118. random primers or oligodT - (reply: 4)
  119. To design or use published primers? - (reply: 4)
  120. PCR primer usage (Clonning & cDNA) - (reply: 2)
  121. problem in cloning PCR primer design with restriction site - (reply: 4)
  122. Amplification with U primers, but not with M primers - (reply: 1)
  123. His tag introduction in to gene and primer design - (reply: 2)
  124. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
  125. Design primer from incomplete sequence... - (reply: 2)
  126. Design primer from incomplete sequence... - (reply: 2)
  127. Primer designing for Methylation - (reply: 6)
  128. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
  129. Overlapping sequence PCR primers - (reply: 1)
  130. BSP PCR primer design explained - (reply: 11)
  131. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
  132. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
  133. good 16S univ. primer for qPCR? - (reply: 3)
  134. I need help for designing a pair of primers for this sequence. - (reply: 1)
  135. long primers PCR - (reply: 4)
  136. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
  137. Sequencing Primers and Plasmids - (reply: 3)
  138. Primers to amplify Kan operon from pet28 - (reply: 2)
  139. Tagged primers and a second PCR targetting the tags - (reply: 5)
  140. Barcode generator for PCR primers - (reply: 2)
  141. Biotinylated primers - (reply: 4)
  142. Transcription of PCR products without a primer coupled with promotor sequence - (reply: 4)
  143. How to join two sequencing files taken form forward and reverse primer - (reply: 3)
  144. Control primers for bisulfite-converted DNA - (reply: 3)
  145. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
  146. Is 27F and Anti-Gamma Primer the same thing for 16s? - (reply: 9)
  147. Q regarding primer mix for MSP!! - (reply: 4)
  148. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  149. BSP Primers with M13 Tails - (reply: 1)
  150. issues when designing PCR primers using PRIMER3 - (reply: 8)
  151. Primer catalog/databasing - (reply: 1)
  152. Primer Blast - (reply: 6)
  153. Help designing primers for use in In-fushion cloning - (reply: 4)
  154. Primers Freeze-Thaw - (reply: 16)
  155. primer design using 16s rRNA - (reply: 1)
  156. Primer concentration for cDNA synthesis using GSP - (reply: 1)
  157. Primer Design HELP! - (reply: 3)
  158. Bisulfite PCR doesn't work and the primer seems abnormal - (reply: 6)
  159. How are Primers made? - (reply: 9)
  160. designing methylation specific primers - (reply: 3)
  161. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  162. Primer Annealing Temperatures - (reply: 24)
  163. same primers, different product in conventional PCR and qPCR - (reply: 6)
  164. Different primer optimalization for nested vs direct MSP? - (reply: 3)
  165. Primers amplify genomic DNA but not cDNA - (reply: 2)
  166. Primer BLAST - (reply: 1)
  167. PCR primer design - published primers trustable? - (reply: 8)
  168. question about universal and specific primers for miRNA real time pcr - (reply: 1)
  169. Can we use degenerate bases in BSP primers? - (reply: 1)
  170. How to check primer sequences using BLAST and other tools? - (reply: 2)
  171. Primer Check? - (reply: 2)
  172. Validation of PCR primers/ probes - (reply: 6)
  173. Two reverse primer sequences for a single forward primer - (reply: 1)
  174. PCR with more than one primer - (reply: 1)
  175. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  176. RT-PCR primer design - (reply: 1)
  177. No or very weak band using 16s primers - (reply: 1)
  178. No PCR amplification with b-actin primers - (reply: 1)
  179. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
  180. Primer Design Help: GFP primers for Arabidopsis - (reply: 1)
  181. No PCR amplified with long primers - (reply: 10)
  182. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  183. another primer dilution question - (reply: 2)
  184. Primer Efficiency - (reply: 2)
  185. oligo(dt) 15 vs random primers - (reply: 3)
  186. primer dilutions - (reply: 4)
  187. Genotyping PCR primers - (reply: 1)
  188. MSP Primers not working - (reply: 6)
  189. Primers too dilute - (reply: 1)
  190. Cloning HELP- Possible Primer issues - (reply: 6)
  191. PCR primer pairs - university exam - (reply: 7)
  192. Help with primer design through PRIMER3 - (reply: 4)
  193. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  194. Tm of primers - prediction and verification - (reply: 1)
  195. how to design primers for 16sr RNA - (reply: 24)
  196. Help with primer design - (reply: 3)
  197. Primer Tm calculation. - (reply: 1)
  198. Running Primers on Agarose Gel - (reply: 6)
  199. PCR product now not getting with same primers and other PCR conditions - (reply: 4)
  200. universal primers for PCR ribotyping - (reply: 6)
  201. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  202. Primer design: Tm above or below Ta? - (reply: 5)
  203. Oligo primers for shRNA construction - (reply: 1)
  204. Design primers for expression cloning - (reply: 11)
  205. primer dilution query - (reply: 2)
  206. Using BLAST to check primers - (reply: 1)
  207. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  208. What is the function of a third primer in a PCR mix?? - (reply: 2)
  209. How can we design a primer ? step by step method - (reply: 4)
  210. EPIGENETICS-NEWBIE-Weird/too close primers for pyrosequencing assay - (reply: 2)
  211. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
  212. Is it possible to amplify cDNA with hexamer primers or oligo dT primers to have - (reply: 1)
  213. annealing temperature for 3 primer pairs? - (reply: 4)
  214. Impact of primer sequence on TA cloning - (reply: 3)
  215. Pcr primers - (reply: 2)
  216. RT-Q PCR primer design question - (reply: 2)
  217. multiplex rtqPCR and custom primer/probe design - (reply: 2)
  218. big differnece in my primers Tm - (reply: 2)
  219. Designing primers for the ORFs - (reply: 9)
  220. PCR with phophorylated primers - (reply: 1)
  221. Problem with New qPCR primers - help! - (reply: 6)
  222. Designing primers for miRNA located on minus strand. - (reply: 2)
  223. Plasmid sequencing without primers - (reply: 1)
  224. RT-PCR primers and the poly(A) tail - (reply: 2)
  225. overlapping primer - (reply: 1)
  226. PCR primers help - (reply: 5)
  227. Primer dimers - (reply: 2)
  228. Primer validation and PCR efficiency - (reply: 4)
  229. should I design primers spanning both intron and exon - (reply: 2)
  230. PCR with a very long and a short primer - (reply: 5)
  231. Comparison between two primers pairs? Is it possible? - (reply: 3)
  232. Rt-PCR primer design - (reply: 7)
  233. Length of non-binding sequence in primers with RE site - (reply: 6)
  234. Orientation of primers - (reply: 2)
  235. methylation analysis with MS-HRM (primer design) - (reply: 1)
  236. primer resuspension - (reply: 2)
  237. Help designing primers with restriction sites - (reply: 3)
  238. Why primers shouldn't have Tm difference greater than 5C? - (reply: 5)
  239. PCR primer with no extra DNA end - (reply: 5)
  240. Tm calculation for primers with RE sites and overhangs - (reply: 4)
  241. Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
  242. checking msp primers - (reply: 2)
  243. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  244. Another primer dimers problem - (reply: 23)
  245. MSP U primers very difficult to amplify... - (reply: 4)
  246. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
  247. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  248. Primer design - (reply: 3)
  249. single primer PCR - (reply: 5)
  250. Primer concentration - (reply: 4)
  251. Primer 3' mismatch - Strange experience (reply: 9)
  252. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
  253. PRIMER DILUTION - STOCK SOLUTION TO A WORKING STANDARD (reply: 2)
  254. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
  255. Are my MSP primers good? - MSP primers design by Methyl Primer Express v1.0 (reply: 2)
  256. Primer dilution --> problem.. - (reply: 16)
  257. Sybr green RT-qPCR primer - (reply: 1)
  258. Sybr green RT-qPCR primer - (reply: 1)
  259. primer design - microalgae (reply: 2)
  260. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
  261. how to overcome primer contamination - need protocol (reply: 11)
  262. RT-PCR primer design - Primer design and evaluation (reply: 7)
  263. STANDARDISATION OF MULTIPLEX PCR - IN order to standardise a multiplex PCR for 3 set of primers (reply: 3)
  264. Primer,probes dilution - (reply: 3)
  265. PCR: Cloning Primers - (reply: 8)
  266. Exon Spanning PCR Primers - (reply: 1)
  267. Primer sequences - (reply: 1)
  268. PCR Primers - (reply: 2)
  269. How to blast methylation specific and unspecific primers - (reply: 1)
  270. How to check primers are of correct sequence or not? - (reply: 2)
  271. Primers... - (reply: 1)
  272. When are primer dimers a problem? - (reply: 1)
  273. primer with higher melting temperature - (reply: 2)
  274. Problems designing primers for BSP - (reply: 2)
  275. qPCR primer design - possible off-target (reply: 1)
  276. Primer dimer formation and real-time PCR - (reply: 7)
  277. Primer Design Urgent! - (reply: 1)
  278. how to know my primer's sense ??? - help ! (reply: 2)
  279. intron spanning primers for non model organisms - (reply: 2)
  280. Primer design - (reply: 2)
  281. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
  282. Primer seq - (reply: 2)
  283. oligo dt and random primer - (reply: 3)
  284. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
  285. Primers and self and hetero dimers - (reply: 1)
  286. Primers for qPCR - (reply: 3)
  287. RT-PCR primer - (reply: 3)
  288. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
  289. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  290. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  291. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  292. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  293. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  294. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
  295. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
  296. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
  297. ChIP primer design - (reply: 1)
  298. Bisulfite sequencing primer design query (probably simple) - (reply: 4)
  299. Question about Primers - (reply: 3)
  300. Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
  301. designing MSP primers (dimers NOOOOOOOO!) - (reply: 1)
  302. Primer design with a tag - (reply: 4)
  303. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
  304. Combining forward and reverse primer gives different size on gel - (reply: 2)
  305. primers for fungal identification - (reply: 9)
  306. difference between PCR primers and sequencing primers - (reply: 1)
  307. Sense and antisense DNA, and primer design - (reply: 6)
  308. Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
  309. Question about RealTime PCR Primer Design - (reply: 1)
  310. Designing a Primer with a RS that has a W - (reply: 1)
  311. Primers stopped working!? - PCR Primers (reply: 6)
  312. NFK beta primers - (reply: 4)
  313. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
  314. Primer design - Primer design (reply: 2)
  315. Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
  316. Real Time PCR Primers and Probes - (reply: 2)
  317. Need help - How we can design primer for miRNA (reply: 1)
  318. primer and promoter sequences - (reply: 1)
  319. designing of primers - (reply: 2)
  320. Primer Tm differences/ PCR - (reply: 1)
  321. Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
  322. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
  323. d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
  324. Designing primer to remove his-tag - (reply: 1)
  325. PCR Primer Dilution from F+R - (reply: 1)
  326. primer reconstitution - primer reconstitution (reply: 4)
  327. Primer design for insert amplification - Is this correct? (reply: 2)
  328. HRM primers - (reply: 1)
  329. primer design - (reply: 2)
  330. New to sequencing, primer design - (reply: 2)
  331. Quickchange mutagenesis primer - (reply: 3)
  332. pcr / primer theory in bisulfite sequencing - (reply: 4)
  333. Primer Design..need very basic advice. - Please help, I cant figure this out for the life of me. (reply: 2)
  334. Problems with the Specificity of the Primers - (reply: 2)
  335. confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
  336. Degenerate primers PCR problem, Please help! - (reply: 2)
  337. Primer design help needed - (reply: 1)
  338. Primers no longer work - (reply: 1)
  339. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
  340. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
  341. Calculating Primer concentrations for PCR - Is there an easy way to do this...help (reply: 3)
  342. primer Tm is too high, how tu get pcr product - help! (reply: 21)
  343. Find universal primers in vector - any online tool? (reply: 6)
  344. Picking primers to confirm Illumina meth27 results - (reply: 2)
  345. Colony Pcr - primers - (reply: 5)
  346. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
  347. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  348. Primer Dilution Problem - D'oh! (reply: 2)
  349. Is there any software that can be sued to design degenerate primers - (reply: 1)
  350. Primer Decontamination - (reply: 2)
  351. tools for checking non-specific binding of primers -
    (reply: 1)
  352. Primer designed for ARMS PCR - (reply: 2)
  353. methylation specific PCR - Primers - (reply: 1)
  354. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
  355. Primer Design - aligning sequences for probe/primer design (reply: 2)
  356. PCR with one primer - (reply: 1)
  357. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
  358. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
  359. High Tm on primers - cannot get a product - (reply: 8)
  360. NCBI primer blast problem - (reply: 1)
  361. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
  362. primer sequence problem - (reply: 2)
  363. Finding bisulfite PCR primer sequence - (reply: 3)
  364. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
  365. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
  366. How to design primers to check my candidates in a ChiP assay - (reply: 4)
  367. Primer Reconstitution--does temperature matter? - (reply: 5)
  368. Primer Design MSP, BSP, MS-HRM - (reply: 3)
  369. Primers that do not align at regions with SNPs. Where to find SNPs? - (reply: 2)
  370. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  371. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
  372. Primer design for qPCR - (reply: 2)
  373. Primer design for qPCR - (reply: 1)
  374. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
  375. primer design tips - (reply: 3)
  376. design pcr primer - (reply: 1)
  377. PCR with long and complex primers - (reply: 2)
  378. Phospholylation of primers - (reply: 1)
  379. LINE1 OFR2 Primer Design - (reply: 1)
  380. phosphorylation of primers using PNK - (reply: 1)
  381. Primers for yeast (18S) - (reply: 1)
  382. What's the longest overhanging primer seq. you have used? - (reply: 2)
  383. Primer Concentration to lower Ct - (reply: 1)
  384. Primer Concentration Help - (reply: 4)
  385. Primer Degradation - What exactly happens? (reply: 1)
  386. Primers in 2 exons - (reply: 2)
  387. Problems with designing a primer - (reply: 2)
  388. Probe or Primer info for bovine AQP-1 - (reply: 6)
  389. Different sized product for same BS primer! - (reply: 2)
  390. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  391. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
  392. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  393. primers with restriction site - (reply: 1)
  394. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
  395. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
  396. primer design by generunner DG - (reply: 1)
  397. BSP primers design, help please - (reply: 2)
  398. primers with fluoresent dye PCR cycle question - (reply: 4)
  399. Urgent! Sequencing a mixture of ssDNA fragments of the same gene - ssDNA different in size amplified from a single primer (reply: 2)
  400. Problems with validating primers and low expression genes - (reply: 3)
  401. intron/exon spanning primers - (reply: 1)
  402. MSP primer troubles - (reply: 3)
  403. Designing Primers for multiple Isoforms - (reply: 1)
  404. real time pcr melt curve and primer efficiency problem - (reply: 3)
  405. how to design primer for my n-terminal sequence - hot to design primer for my protein (reply: 2)
  406. Addition of Restriction sites into PCR primers - (reply: 4)
  407. Primer Design in 3non translated version vs. coding region - (reply: 2)
  408. different PCR primers for real-time and classic PCR - (reply: 3)
  409. primers deconamination??? - (reply: 6)
  410. primer check!!! - (reply: 6)
  411. Annealing Temperature of biotinylated primers - (reply: 2)
  412. primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
  413. Primer clean-up - ExoSAP-It isn't working (reply: 6)
  414. Primer dilution - PCR primer dilution (reply: 2)
  415. Long Primers for PCR - (reply: 2)
  416. Primers going "loopy" - (reply: 2)
  417. Conversion of primer unit - ug/ul to uM (reply: 4)
  418. Primer Trouble Shooting - (reply: 5)
  419. Proven Primers Stopped working & now leave smear! - (reply: 2)
  420. a simple primer problem - (reply: 5)
  421. Primers from 3'UTR - (reply: 2)
  422. Designing primers with ESTs - (reply: 2)
  423. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
  424. addition of BclI restriction site to PCR primer - (reply: 6)
  425. How does methprimer calculate primer Tm - (reply: 2)
  426. Phosphorylated primers - (reply: 5)
  427. QPCR - Primer and Probe question - QPCR (reply: 2)
  428. Easy primer question? - Primers (reply: 1)
  429. primer dimer - proplems (reply: 1)
  430. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
  431. Primer efficiency test - (reply: 1)
  432. primer software - (reply: 2)
  433. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
  434. How to know in which exon a primer match? - (reply: 1)
  435. primer annealing - higher annealing= less wrong bounds (reply: 4)
  436. sequencing with forward/reverse primers - (reply: 7)
  437. Adding A overhangs - primer design implications? - (reply: 1)
  438. primer RE over-hang nucleotides - common sequences? (reply: 5)
  439. Forward and reverse primers got very different Tm - what to do? (reply: 11)
  440. primer design problem - (reply: 1)
  441. Problem with primer efficiency analysis - (reply: 4)
  442. primer design - (reply: 1)
  443. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
  444. designing primers for genes not sequenced yet - (reply: 2)
  445. Primer desing - (reply: 2)
  446. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
  447. primer design - (reply: 9)
  448. primer contamination - primer contaminated with ice (reply: 3)
  449. Primer3 vs Primer BLAST - (reply: 3)
  450. Primer design: free energy - (reply: 2)
  451. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  452. Primer optimization for ChIP - (reply: 2)
  453. sequencing primer: T7 or T7promoter? - (reply: 4)
  454. primer tm - tm calculation for tagged primers (reply: 2)
  455. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
  456. Universal 16s rRNA primers needed - (reply: 3)
  457. PCR primers for histone mod ChIP - How to find the regions (reply: 1)
  458. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
  459. adding koazak sequence to primer - (reply: 1)
  460. Designing primers for cloning - After primer designing, how should I perform the PCR? (reply: 15)
  461. Primer design if sequence is unknown for organism - (reply: 5)
  462. adding C terminal tag to reverse primer - (reply: 1)
  463. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
  464. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
  465. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
  466. what primers should I use for DNA sequencing? please help - (reply: 2)
  467. what primers should I use for DNA sequencing?? - (reply: 3)
  468. Is my primer going to work for qRT-PCR? - (reply: 6)
  469. Primers annealing temperatures - (reply: 8)
  470. Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
  471. Synthesize own Oligo-dT primers - (reply: 6)
  472. Testing primer specificity... - ...without gDNA of target organisms (reply: 6)
  473. primer design - Primer design for PCR (reply: 1)
  474. How are primers designed? - (reply: 4)
  475. need help on primer calcuation soon - (reply: 1)
  476. primer design - (reply: 6)
  477. 23S rRNA Primer - need primer sequence (reply: 1)
  478. Freezing primers+SYBR green - (reply: 2)
  479. Tagged Primers - (reply: 5)
  480. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
  481. design primer for chip - (reply: 5)
  482. How to quantify oligonucleotide primers using nanodrop ND-1000 software - newbie help (reply: 2)
  483. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  484. reconstitution of primers - (reply: 2)
  485. primer dimer - (reply: 2)
  486. PCR with long primers - (reply: 3)
  487. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  488. Help me! How to design nested BSP primers? - (reply: 4)
  489. Using BLAST to check primers - (reply: 17)
  490. PCR primer - (reply: 2)
  491. Primer design - GC clamp - (reply: 8)
  492. primer dimer or what? - (reply: 2)
  493. Primers and Taqman Probes mixture question - (reply: 1)
  494. designing primers - (reply: 1)
  495. Negative Control Primers for ChIP Assay - (reply: 3)
  496. PCR Cloning-large primers - (reply: 4)
  497. forward and reverse primer - (reply: 4)
  498. Check the primers - (reply: 4)
  499. BSP primers - (reply: 2)
  500. Primer dimer issue in real time PCR - (reply: 21)
  501. RT-PCR primer dimers and cDNA degradation - (reply: 2)
  502. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  503. primer binding - (reply: 1)
  504. weird ChIP primers! - (reply: 1)
  505. PCR primers - (reply: 1)
  506. diluting to final primer concentration - please help correct (reply: 1)
  507. Smear problem with my new primer set - (reply: 2)
  508. Amplifying a gene using a degenerate primer - (reply: 4)
  509. Making Primer Dimers on Purpose - odd I know...but humor me (reply: 5)
  510. Primer Design - Design Primer with DNA Sequence (reply: 1)
  511. PCR arrays=Primers in a plate? - Can any PCR mix be used? (reply: 1)
  512. Primer tm - (reply: 1)
  513. primer design - really necessary? (reply: 2)
  514. Degenerative primers = multiple products? - (reply: 5)
  515. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
  516. Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
  517. primer dimers - (reply: 5)
  518. qPCR primer design - (reply: 5)
  519. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
  520. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
  521. mouse Gapdh chip-qPCR primers for control! - (reply: 7)
  522. Primer stock confusion - (reply: 6)
  523. primer design - primer design question (reply: 2)
  524. Determine annealing temperature of primers - (reply: 14)
  525. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
  526. Validating real time pcr primers - (reply: 2)
  527. BSP,MSP primer design? - (reply: 1)
  528. low gc primers - help with pcr using low gc primers (reply: 6)
  529. Primers and annealing temperature - (reply: 2)
  530. WHich primer to use for sequencing - (reply: 5)
  531. primer design for pBAD topo expression kit - (reply: 2)
  532. primers fpr sequencing - (reply: 4)
  533. PCR with pfu and degenerate primers (?) - (reply: 7)
  534. Draw PCR primer locations - PCR primer (reply: 4)
  535. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
  536. Program for mapping primers to gene - diagram maker, visualize - (reply: 4)
  537. long PCR primer - (reply: 7)
  538. Taqman rtPCR primer and probe design - (reply: 3)
  539. Amplifying plasmid - non specific binding of primer - (reply: 3)
  540. primer design - (reply: 3)
  541. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
  542. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  543. design primer - when I design a primer is it necessary to include the restriction (reply: 1)
  544. Difference in Primer Melting Temperatures - Maximum allowable Tm difference? (reply: 4)
  545. Primers Not working - Real time PCR using SYBR Green (reply: 3)
  546. PCR conditions with three primers - (reply: 1)
  547. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  548. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
  549. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
  550. using the meth primer - (reply: 2)
  551. Generating primers for MSP - (reply: 1)
  552. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  553. Primer design and need help - (reply: 11)
  554. Programme for degenerate primer design - Programme for primer design (reply: 9)
  555. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  556. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)