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Extraction Related Discussions
  1. multiple bands for genomic DNA extraction - (reply: 2)
  2. Phase separation during DNA extraction - (reply: 5)
  3. Protein extraction from dead cells - (reply: 1)
  4. Storage of tissue at -80 followed by a breif unthaw prior to extraction - (reply: 1)
  5. PLEASE HELP: phenol-chloroform extraction and EtOH precipitation problem - (reply: 4)
  6. Is it possible to extract RNA from Cells frozen in 10% DMSO ? - (reply: 2)
  7. Extracting DNA from fruits and Vegetables - (reply: 8)
  8. How to lysis cells for an RNA extraction - (reply: 1)
  9. protein solubilization and quantitation after extraction using Trizol - (reply: 2)
  10. I forgot treat my samples with proteinase K before the DNA extraction step - (reply: 1)
  11. mitochondrial DNA extraction from avian blood -urgent - (reply: 5)
  12. Urgent - RNA extraction / RNase contamination ? - (reply: 3)
  13. protein extraction from transwell inserts - (reply: 2)
  14. Weird results for gDNA extraction... - (reply: 5)
  15. 2-D electrophoresis of sperm membrane extracts - (reply: 9)
  16. result of plasmid extraction - (reply: 2)
  17. plasmid extraction - (reply: 5)
  18. agarose gel electrophoresis of plasmid extraction - (reply: 12)
  19. plasmid extraction - (reply: 3)
  20. RNA extraction protocol help - (reply: 1)
  21. histone proteins extraction and 2D - (reply: 1)
  22. plasmid extraction - (reply: 13)
  23. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
  24. Mortar and Pestle for RNA Extraction - (reply: 1)
  25. Extraction of Lactase from lactobacillus - (reply: 2)
  26. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
  27. Optimizing Trizol mRNA extraction - (reply: 3)
  28. dna extraction from plant tissue infected with fungus - (reply: 1)
  29. sample preservation for RNA extraction - (reply: 1)
  30. miRNA extraction from cell-conditioned media? - (reply: 1)
  31. RNA Buffer Extraction - (reply: 3)
  32. Quiagen DNA extraction kit problem - (reply: 3)
  33. Need help with extracting miRNA from human serum using mirVana kit - (reply: 2)
  34. No protein in cytoplasmic extract - (reply: 1)
  35. protein extraction from pirmary keratinocytes - (reply: 2)
  36. Viral RNA Extraction from Tissue Culture Supernatant - (reply: 5)
  37. DNA extraction from cerebrospinal fluid using trizol - (reply: 2)
  38. Nuclear extract problems - (reply: 3)
  39. nuclear extraction question - (reply: 6)
  40. DNA and RNA extraction - (reply: 6)
  41. RNA extraction of ethanol preserved matherial - (reply: 1)
  42. RNA extraction from paraffin embedded tissue - (reply: 2)
  43. RNA extraction from a 1mm brain tissue punch - uncertain about homogenisation - (reply: 2)
  44. total RNA Extraction gel control - (reply: 1)
  45. Chloroform/Isoamyl Alcohol extraction. Problem with phases separation. - (reply: 5)
  46. DNA extraction and paraffin embedded tissue - (reply: 5)
  47. sample storage method for extracting 16s rRNA - (reply: 4)
  48. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  49. How to determine the volume of lisis buffer and DNA extraction buffer when the a - (reply: 5)
  50. No band after extracting DNA from bacillus subtilis - (reply: 1)
  51. Bacillus subtilis DNA extraction protocol - (reply: 7)
  52. Ammonium acetate DNA extraction mistake - (reply: 1)
  53. no dna after extraction - (reply: 5)
  54. applying cell culture protocol of protein extraction for rat intact tissue is co - (reply: 1)
  55. Tenfold PBS in extraction - (reply: 3)
  56. RNA extraction and purity from Drosophila - (reply: 1)
  57. Extracting DNA from FACS sorted cells - (reply: 3)
  58. How much cytoplasmic and nuclear extracts load in a WB? - (reply: 2)
  59. How to extract total protein from gram negative bacteria for SDS page and BCA an - (reply: 2)
  60. extract plasmids from transfected eukaryotic cells - (reply: 6)
  61. biotinylated protein extracts and antigen-antibody stringency - (reply: 3)
  62. RNA extraction from human sperm - (reply: 3)
  63. Protein extraction efficiency - (reply: 2)
  64. Trizol and Protein extraction - (reply: 2)
  65. Protein Extraction SDS-Buffer Pellet not Dissolving and Laemlli Bufffer Green? - (reply: 3)
  66. RNA extraction and preparation - RTqPCR - (reply: 3)
  67. PAXgene blood RNA tube for RNA extraction - (reply: 1)
  68. Difference between plasmid DNA extraction and genomic DNA extraction - (reply: 3)
  69. DNA extraction and blue dextran as a DNA carrier - (reply: 4)
  70. cytoplasmic and nuclear extraction - problem with contamination! - (reply: 2)
  71. CTAB extraction problem - CTAB extraction problem (reply: 2)
  72. chloroform/Qiagen Dneasy plant mini kit - DNA extraction (reply: 1)
  73. DNA extraction of microalgae - microalgae (reply: 3)
  74. DNA extract quality - (reply: 2)
  75. confirm proteins interacting with histones using acid extraction - (reply: 4)
  76. DNA extraction from liver - (reply: 3)
  77. DNA extraction - we want to extract DNA from bone marrow smear (reply: 2)
  78. The Best Gel Extraction Kits - The Best Gel Extraction Kits (reply: 9)
  79. could i use sterile deionized water instead of DEPEC water in RNA extraction - (reply: 6)
  80. Dna after gel extraction, single or double stranded? - (reply: 1)
  81. Whole blood RNA and DNA extraction help - Expression analysis and epigenetics (reply: 2)
  82. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
  83. Extraction of 12 bp DNA from polyacrylamide gel - (reply: 2)
  84. mass spectra of methanolic extract of withania somnifera - (reply: 4)
  85. RNA extraction from PBMC layer - how to increase RNA Concentration during RNA extraction from BLOOd (reply: 1)
  86. Nuclear lysis followed by histone extraction - (reply: 3)
  87. dna extraction question - (reply: 2)
  88. Gel Extraction using buffer QG then phenol/chl - Can I? (reply: 1)
  89. Active protein extract from corn - (reply: 2)
  90. oocye extraction and RNA isolation - (reply: 1)
  91. Bacterial DNA Extraction - possible of contamination? - (reply: 7)
  92. 1-day old culture for DNA extraction - plausible? - (reply: 2)
  93. Lactobacillus crude protein extraction? - (reply: 8)
  94. paraffin from ffpe tissue samples refuse to dissolve in xylene - DNA extraction from ffpe tissue shavings (reply: 2)
  95. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
  96. Heterologous protein extraction in yeast for enzymatic activity test - (reply: 5)
  97. Extracting Plasma from frozen Blood samples - (reply: 4)
  98. DNA extraction from E. coli - solutions needed? (reply: 6)
  99. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
  100. RNA extraction - (reply: 3)
  101. Nuclear extract troubleshooting - (reply: 1)
  102. Lipid extraction from bacteria - Lipid extraction from bacteria (reply: 1)
  103. EMSA - probe disappears with nuclear extract - probe works without nuclear extract... (reply: 1)
  104. RNA extraction troubleshooting: low 260/230 - (reply: 1)
  105. cherry leaf extracts - how to avoid gummy stuff in leaf extract (reply: 2)
  106. RNA extraction from FTA classic - (reply: 1)
  107. how to extract proteins from HEK293 - I need your experience (reply: 3)
  108. nuclear extraction of blood cells - (reply: 2)
  109. RNA extraction Question - (reply: 2)
  110. Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
  111. Homogenization of articular cartilage prior to protein extraction - (reply: 1)
  112. PCR, using gel extracted band as a template - (reply: 4)
  113. Question of nucleic acid extraction - (reply: 1)
  114. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  115. How to isolate serum from blood for RNA extraction - (reply: 2)
  116. DNA extraction from plated colonies - (reply: 3)
  117. nuclear extraction insoluble pellet problem - here it is again! (reply: 2)
  118. Difficult to extract DNA from vulcanic coastal sediments - (reply: 2)
  119. Nuclear Extraction headache - persistent pellet glob - (reply: 9)
  120. Calculating - RNA extraction (reply: 3)
  121. Protein extraction from perfusion fixed brain tissue - (reply: 5)
  122. Prepare p:C:I - for RNA extraction (reply: 5)
  123. Nuclear extraction on non-primary culture cells - Western blot (reply: 2)
  124. genomic DNA extraction: up to 50kb? - genomic DNA isolation (reply: 1)
  125. Homogenization for Plasma Membrane Protein Extraction - (reply: 1)
  126. Isoamyl Alcohol in DNA extraction - (reply: 3)
  127. cytoplasmic and nuclear extraction for BCA - (reply: 6)
  128. Smears above ribosomal RNA bands - RNA extraction from microalgae (reply: 2)
  129. Trizol Extraction of PBMCs, low 260/230 - (reply: 5)
  130. TRIzol phase extraction with Lock-Phase tubes - Does anyone use Lock-Phase? (reply: 1)
  131. Gel Extracting a 15kb Band? - Quick and Easy method (reply: 3)
  132. DNA extraction from paraffin embbed tissue - How can extract DNA form tissue (reply: 2)
  133. Saliva DNA extraction help - (reply: 3)
  134. DNA Extraction from bacterial cultures - (reply: 3)
  135. Nucleic Acid interference in Western Blot / Optimal Nuclear Extract Protocol - Nuclear Extract Optimization (reply: 4)
  136. Extraction of bacterial DNA - (reply: 1)
  137. Protein extraction from mouse prostate - (reply: 3)
  138. Qiagen Extraction Kits - (reply: 1)
  139. Molecular Biology methods: Nucleic Acid extraction - RNA extraction (reply: 2)
  140. gDNA had smear band - problem in extraction human genomic DNA (reply: 2)
  141. midiprep - plasmid extraction (reply: 1)
  142. DNA extraction from induced phage - (reply: 1)
  143. Large DNA extraction from an agarose gel - (reply: 8)
  144. DNA isolation from plant nuclei by plasmid extraction kit - (reply: 1)
  145. Viral DNA extraction - (reply: 1)
  146. Extraction protein from paraffin-embedded tissues - (reply: 1)
  147. nuclear extraction problem - nuclei gets too clumpy - (reply: 3)
  148. mite DNA extraction - (reply: 7)
  149. how to do histone extraction - (reply: 1)
  150. mite DNA extraction - (reply: 1)
  151. Looking for recommendation for total RNA extraction kit - (reply: 4)
  152. how to extract enzyme allinase from garlic - (reply: 1)
  153. Lipid extraction with ORO - (reply: 2)
  154. Is Histone H1 suitable to check the purity of cytoplasm extracts? - (reply: 1)
  155. RNA extraction protocol for small amounts of cells - (reply: 2)
  156. Problem with gel extraction of DNA. - (reply: 1)
  157. gDNA extraction with pestle and mortar without liquid nitrogen? - (reply: 5)
  158. how to prepare garlic extract for GC? - (reply: 1)
  159. How to extract restriction enzyme (or restriction endonuclease) from bacteria? - (reply: 1)
  160. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
  161. RNA extraction from stained cells - (reply: 2)
  162. Harvesting Mouse Organs for Mouse Tissue Extracts - (reply: 2)
  163. problem with Trizol RNA extraction - (reply: 7)
  164. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
  165. Phenol extraction - (reply: 21)
  166. nucleic acid extraction from silenced cells - (reply: 6)
  167. DNA Extraction - (reply: 1)
  168. crude or extract which is better - (reply: 6)
  169. RNA extraction from trout embryos - (reply: 3)
  170. Lipid raft extraction HELP! - (reply: 2)
  171. DNA extraction - (reply: 2)
  172. need lysis buffer for histone extraction - (reply: 1)
  173. DNA extraction form endoscopy samples - (reply: 4)
  174. Functions of this reagents!!! - Please help me with DNA extraction!!! (reply: 1)
  175. RNA extraction expertise needed! - (reply: 2)
  176. Protein extraction buffer compatible with 2D PAGE - (reply: 4)
  177. Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
  178. IP from nuclear extract - (reply: 3)
  179. PCR/DNA Extraction Problem - (reply: 5)
  180. Extraction of DNA smaller than 100 bp from Agarose Gel - (reply: 6)
  181. DNA extraction - (reply: 1)
  182. question about plasmid extraction - (reply: 2)
  183. Sample handling for RNA extraction - (reply: 1)
  184. Isobutanol extraction of DNA - (reply: 2)
  185. peptide extraction tricine gel - (reply: 1)
  186. Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
  187. Nuclear extraction - (reply: 2)
  188. DNA extraction from very small volumes - (reply: 5)
  189. dna extraction from soil samples - (reply: 4)
  190. Can I clean up Phenol:Chloroform extraction with a column? - (reply: 3)
  191. cytoplasmic and nuclear extraction - (reply: 2)
  192. RNA extraction - (reply: 2)
  193. Help with protein extraction buffer problem!? - (reply: 1)
  194. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
  195. Purification of Protein Extract for ELISA - (reply: 1)
  196. Trizol RNA extraction - (reply: 2)
  197. Extraction of RNA from blood samples - Problems (Trizol method) (reply: 1)
  198. Fungal DNA extraction from wheat - (reply: 5)
  199. which is the best lysis buffer for whole cell extracts? - (reply: 1)
  200. Freeze drying prior to DNA extraction - (reply: 1)
  201. DNA extraction from mouse tail - (reply: 2)
  202. extraction of viral rna from plant tissue - (reply: 1)
  203. plasmid extraction kit wash buffer solution - (reply: 3)
  204. plasmid extraction kit solution composition - wash buffer (reply: 2)
  205. DNA Extraction - (reply: 2)
  206. protein extraction from tissue, help - (reply: 1)
  207. Protein extraction from tissue - (reply: 1)
  208. Transport & storage of protein extracts - (reply: 1)
  209. Mitochondria extraction from human liver cells - (reply: 1)
  210. problems with qiagen gel extraction kit - (reply: 7)
  211. How does high salt extraction of nuclear proteins work - (reply: 9)
  212. Nuclear Protein Extraction - Nuclear Protein Extraction (reply: 1)
  213. Can you store cell pellets for future nuclear extraction? - (reply: 3)
  214. Storage of gel extracted DNA before ligation - (reply: 3)
  215. total RNA (for miRNA) extraction from human serum - (reply: 11)
  216. how to extract protein from agarose? - (reply: 1)
  217. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
  218. Low RNA yield after extraction by RNA blood mini kit. - (reply: 2)
  219. Problems with nuclear extraction - (reply: 1)
  220. Help for some general DNA Extraction Questions - (reply: 5)
  221. Extracting precious tissue from too large of volumes of RNAlater - stored tissue in too large of volume of RNAlater, can't recover (reply: 5)
  222. PLASMA RNA extraction - (reply: 1)
  223. DNA contamination after RNA extraction, which kit works best? - (reply: 6)
  224. Optimal growth of Lactobacillus using yeast extract - (reply: 2)
  225. Sterilization - Sterilization of herbal extract (reply: 5)
  226. DNA extraction from FFPE? - (reply: 2)
  227. Phenol chloroform extraction - (reply: 2)
  228. Is there a way to "rescue" an already-completed extraction from PCR in - (reply: 1)
  229. MYCOPLASMALOGY - MYCOPLASMA DNA EXTRACTION (reply: 7)
  230. EXtraction of DNA bands from acrylamide gel - (reply: 4)
  231. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
  232. best method for DNA extraction - (reply: 3)
  233. Protein extraction from plant leaves, bioactivity - (reply: 1)
  234. Extract collagen from rat tail (in lab) - (reply: 1)
  235. collagen extraction - (reply: 1)
  236. Rescue my DNA extraction - (reply: 1)
  237. membrane protein extraction buffer - (reply: 1)
  238. bacteria lysis - E coli lysis for recombinant protein extraction (reply: 1)
  239. Intestine histology slide extraction and storage - (reply: 1)
  240. Using analytical grade reagents for DNA extraction - (reply: 6)
  241. Evaluate My Protocol: RNA Extraction w/ Fat. - (reply: 1)
  242. Using DNA in gel extract kept at -20ºC - (reply: 2)
  243. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
  244. Help with Phenol-Chloroform DNA Extraction - (reply: 3)
  245. RNA Extraction Mice Adipose Tissue - (reply: 2)
  246. Direct PCR (PCR without DNA extraction) - (reply: 2)
  247. RNA extraction 260/230 ratio - (reply: 6)
  248. Nucleospin Extract II kit also for plasmids? - (reply: 5)
  249. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
  250. DNA extraction with expired kit? - (reply: 1)
  251. nuclear extract -- HEPES pH?! - (reply: 1)
  252. extracting protein from trizol - (reply: 2)
  253. [vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)
  254. How much sonication power needed? - protein extraction from cells (reply: 2)
  255. Blue Juice loading dye & Gel extraction kit - HELP :( (reply: 6)
  256. protein extraction - from cell line in T25 flask (reply: 5)
  257. help-how to extract fluo-labeled DNA from agarose gel - (reply: 1)
  258. protein extraction using trizol - can we replace guanidine cholride with other denaturants? (reply: 1)
  259. protein precipitation with TCA for dna extraction? - (reply: 1)
  260. A260/230 readings post gel extraction - (reply: 19)
  261. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
  262. Extraction of protein from sample buffer (+DTT) - (reply: 1)
  263. DNA extraction fron HSV-1 infected Vero cell - (reply: 2)
  264. low DNA yield after extraction - (reply: 1)
  265. protein precipitation without phenol/chloroform for dna extraction? - (reply: 1)
  266. Help: Advices on extracting RNA from pellets? - (reply: 3)
  267. How to extract protein from SDS-PAGE gel? - (reply: 7)
  268. Best & Most Popular Kits - for lysis, extraction, etc... (reply: 2)
  269. protein extraction from primary cells - (reply: 1)
  270. Extracting proteins from cells - (reply: 1)
  271. Minimal amounts of whole cell extracts for Co-IP - (reply: 1)
  272. Gas Chromatography - Lipid extraction and derivitization (reply: 1)
  273. Trizol RNA/DNA extraction - (reply: 3)
  274. methanolic extract of withania somnifera (ashwagandha) - (reply: 7)
  275. Plant RNA extraction problem - A cry for help. (reply: 2)
  276. failed PCR on DNA extract from blood - (reply: 6)
  277. Phenol - why different for DNA and RNA extraction? (reply: 9)
  278. Gel extraction help.... desperate! - (reply: 6)
  279. Protein Extraction from Human Tissue/Tumor Samples - (reply: 1)
  280. DNA extraction and PCR advice requested - PCR, Contamination, Low abundance targets (reply: 7)
  281. Problem with DNA extraction from 1% agarose Gel - No DNA using Qiagen Qiaek 2 and Spin column kits (reply: 9)
  282. protein extraction from tumor samples containing matrigel? - (reply: 2)
  283. Extracting Proteins From Soil - (reply: 3)
  284. Genomic material extraction from a vaccine - (reply: 1)
  285. Dry Plant leaf samples for DNA extraction - (reply: 1)
  286. PCR without DNA extraction! - (reply: 2)
  287. RNA extraction using TRIzol - (reply: 19)
  288. Nuclear Extracts (Golgi contamination) - (reply: 3)
  289. Trizol - RNA extraction (reply: 5)
  290. protein extraction after cell lysing - (reply: 2)
  291. Protein extraction from rat aorta - (reply: 3)
  292. RNA extraction for microarray - (reply: 25)
  293. problem with plasmid extraction - (reply: 2)
  294. DNA extraction from saliva/buccal swabs - (reply: 5)
  295. simultaneous extraction of nucleic acids and proteins - (reply: 3)
  296. Could You Suggest me A good KIt for DNA extraction from Yeast? - (reply: 1)
  297. DNA extraction trouble - (reply: 7)
  298. gel extraction protocol for small amount of starting material - (reply: 3)
  299. Extraction of large size genomic DNA - (reply: 3)
  300. Phenol chloroform extraction of DNA - (reply: 3)
  301. ELISA Extraction Buffer for Plants? - (reply: 4)
  302. RNA extraction - (reply: 1)
  303. Proteinase K to improve RNA extraction yield - (reply: 2)
  304. cloudy phase during RNA extraction with Trizol - (reply: 10)
  305. Plasmid extraction sans commercial kits - (reply: 3)
  306. Tips for genomic DNA extraction from gram -ve bacteria - (reply: 2)
  307. DNA extraction methods - PCI extrction (reply: 3)
  308. Plant DNA extraction - (reply: 5)
  309. Protein extraction from cell culture media - (reply: 9)
  310. RNA Extraction & EMSA - (reply: 1)
  311. Protein extraction from mouse organs - (reply: 6)
  312. Fast, cheap and dummy proof large scale DNA extraction? - (reply: 6)
  313. Does phenol extraction have effect on DNA methylation? - (reply: 4)
  314. RNA extraction in -80 - (reply: 1)
  315. Selection during Extraction: Plasmid or Genomic DNA? - (reply: 1)
  316. Loading control for nuclear extracts - (reply: 6)