Protocol Online logo
Top : New Forum Archives (2009-): : Tissue and Cell Culture

culturing of freshly extracted PBMCs - (Mar/03/2013 )

Hi there,

Does anyone know how long freshly prepared PBMCs can be kept in cell culture?

Thank you



From my experience with PBMCs, they can be passaged for multiple times and frozen down. For freshly isolated PBMCs, its best to not keep it in RT for too long. You can put them into culture in RPMI-1640 in heat-inactivated serum.

This is a very useful reference to have a look at for PBMC culturing: http://www.ncbi.nlm....les/PMC3010910/

-science noob-